RNASEH1
{{Short description|Protein-coding gene in the species Homo sapiens}}
{{Infobox_gene}}
Ribonuclease H1 also known as RNase H1 is an enzyme that in humans is encoded by the RNASEH1 gene.{{cite web | title = Entrez Gene: ribonuclease H1| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=246243}}{{cite journal |vauthors=ten Asbroek AL, van Groenigen M, Jakobs ME, Koevoets C, Janssen B, Baas F | title = Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome | journal = Genomics | volume = 79 | issue = 6 | pages = 818–23 |date=June 2002 | pmid = 12036296 | doi = 10.1006/geno.2002.6776 }}{{cite journal |vauthors=Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W | title = Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription | journal = Mol. Cell | volume = 28 | issue = 2 | pages = 264–76 |date=October 2007 | pmid = 17964265 | doi = 10.1016/j.molcel.2007.08.015 | doi-access = free }} The RNase H1 is a non-specific endonuclease and catalyzes the cleavage of RNA via a hydrolytic mechanism.
References
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Further reading
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- {{cite journal |vauthors=Nowotny M, Cerritelli SM, Ghirlando R, etal |title=Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD. |journal=EMBO J. |volume=27 |issue= 7 |pages= 1172–81 |year= 2008 |pmid= 18337749 |doi= 10.1038/emboj.2008.44 |pmc=2323259 }}
- {{cite journal |vauthors=Lima WF, Wu H, Nichols JG, etal |title=Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate. |journal=J. Biol. Chem. |volume=278 |issue= 50 |pages= 49860–7 |year= 2003 |pmid= 14506260 |doi= 10.1074/jbc.M306543200 |doi-access= free }}
- {{cite journal |vauthors=Lima WF, Rose JB, Nichols JG, etal |title=The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis. |journal=Mol. Pharmacol. |volume=71 |issue= 1 |pages= 73–82 |year= 2007 |pmid= 17028157 |doi= 10.1124/mol.106.025429 |s2cid=27765676 }}
- {{cite journal |vauthors=Lima WF, Nichols JG, Wu H, etal |title=Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis. |journal=J. Biol. Chem. |volume=279 |issue= 35 |pages= 36317–26 |year= 2004 |pmid= 15205459 |doi= 10.1074/jbc.M405035200 |doi-access= free }}
- {{cite journal |vauthors=Otsuki T, Ota T, Nishikawa T, etal |title=Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. |journal=DNA Res. |volume=12 |issue= 2 |pages= 117–26 |year= 2005 |pmid= 16303743 |doi= 10.1093/dnares/12.2.117 |doi-access= free }}
- {{cite journal |vauthors=Wu H, Lima WF, Crooke ST |title=Properties of cloned and expressed human RNase H1. |journal=J. Biol. Chem. |volume=274 |issue= 40 |pages= 28270–8 |year= 1999 |pmid= 10497183 |doi=10.1074/jbc.274.40.28270 |doi-access=free }}
- {{cite journal |vauthors=Frank P, Braunshofer-Reiter C, Pöltl A, Holzmann K |title=Cloning, subcellular localization and functional expression of human RNase HII. |journal=Biol. Chem. |volume=379 |issue= 12 |pages= 1407–12 |year= 1998 |pmid= 9894807 |doi=10.1515/bchm.1998.379.12.1407 |s2cid=19692186 }}
- {{cite journal |vauthors=Wu H, Lima WF, Zhang H, etal |title=Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs. |journal=J. Biol. Chem. |volume=279 |issue= 17 |pages= 17181–9 |year= 2004 |pmid= 14960586 |doi= 10.1074/jbc.M311683200 |doi-access= free }}
- {{cite journal |vauthors=Hillier LW, Graves TA, Fulton RS, etal |title=Generation and annotation of the DNA sequences of human chromosomes 2 and 4. |journal=Nature |volume=434 |issue= 7034 |pages= 724–31 |year= 2005 |pmid= 15815621 |doi= 10.1038/nature03466 |bibcode=2005Natur.434..724H |doi-access= free }}
- {{cite journal |vauthors=Lima WF, Wu H, Nichols JG, etal |title=Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines. |journal=J. Biol. Chem. |volume=278 |issue= 17 |pages= 14906–12 |year= 2003 |pmid= 12473655 |doi= 10.1074/jbc.M211279200 |doi-access= free }}
- {{cite journal |vauthors=ten Asbroek AL, van Groenigen M, Nooij M, Baas F |title=The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides. |journal=Eur. J. Biochem. |volume=269 |issue= 2 |pages= 583–92 |year= 2002 |pmid= 11856317 |doi=10.1046/j.0014-2956.2001.02686.x |doi-access=free }}
- {{cite journal |vauthors=Gerhard DS, Wagner L, Feingold EA, etal |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 |pmc=528928 }}
- {{cite journal |vauthors=Ota T, Suzuki Y, Nishikawa T, etal |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40–5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 |doi-access= free }}
- {{cite journal |vauthors=Cerritelli SM, Crouch RJ |title=Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI. |journal=Genomics |volume=53 |issue= 3 |pages= 300–7 |year= 1998 |pmid= 9799596 |doi= 10.1006/geno.1998.5497 |url= https://zenodo.org/record/1229778 }}
- {{cite journal |vauthors=Strausberg RL, Feingold EA, Grouse LH, etal |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2002 |pmid= 12477932 |doi= 10.1073/pnas.242603899 |pmc=139241 |bibcode=2002PNAS...9916899M |doi-access=free }}
- {{cite journal |vauthors=Cerritelli SM, Frolova EG, Feng C, etal |title=Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice. |journal=Mol. Cell |volume=11 |issue= 3 |pages= 807–15 |year= 2003 |pmid= 12667461 |doi=10.1016/S1097-2765(03)00088-1 |doi-access=free }}
- {{cite journal |vauthors=Lima WF, Rose JB, Nichols JG, etal |title=Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate. |journal=Mol. Pharmacol. |volume=71 |issue= 1 |pages= 83–91 |year= 2007 |pmid= 17028158 |doi= 10.1124/mol.106.025015 |s2cid=24995048 }}
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External links
- {{PDBe-KB2|O60930|Human Ribonuclease H1 (RNASEH1)}}
{{Esterases}}
{{DNA replication}}
{{gene-2-stub}}