ROSA26

ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice.{{cite web|title=rosa26|url=http://research.mssm.edu/soriano/lab/rosa26.html|accessdate=8 January 2013}} It was first isolated in 1991{{cite journal|pmid=1653172|year=1991|last1=Friedrich|first1=G|last2=Soriano|first2=P|title=Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice|volume=5|issue=9|pages=1513–23|journal=Genes & Development|doi=10.1101/gad.5.9.1513|doi-access=free}} by the group of Philippe Soriano in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 800 knock-in lines have been created based on the ROSA26 locus according to the MGI database.{{Cite web |title=Gt(ROSA)26Sor MGI Mouse Gene Detail - MGI:104735 - gene trap ROSA 26, Philippe Soriano |url=https://www.informatics.jax.org/marker/MGI:104735 |access-date=2023-09-30 |website=www.informatics.jax.org}} The human homolog of the ROSA26 locus has been identified.{{cite journal|doi=10.1038/nbt1362|title=Identification and targeting of the ROSA26 locus in human embryonic stem cells|year=2007|last1=Irion|first1=Stefan|last2=Luche|first2=Hervé|last3=Gadue|first3=Paul|last4=Fehling|first4=Hans Joerg|last5=Kennedy|first5=Marion|last6=Keller|first6=Gordon|journal=Nature Biotechnology|volume=25|issue=12|pages=1477–82|pmid=18037879|s2cid=10292785 }} ROSA stands for Reverse Orientation Splice Acceptor, named after the lentivirus genetrap vector.{{Cite journal|last1=Friedrich|first1=G.|last2=Soriano|first2=P.|date=September 1991|title=Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice|journal=Genes & Development|volume=5|issue=9|pages=1513–1523|doi=10.1101/gad.5.9.1513|issn=0890-9369|pmid=1653172|doi-access=free}}