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RTCB

{{Short description|Protein-coding gene in the species Homo sapiens}}

{{Infobox_gene}}

RNA 2',3'-cyclic phosphate and 5'-OH ligase is a protein that in humans is encoded by the RTCB gene.

{{cite web

| title = Entrez Gene: RNA 2',3'-cyclic phosphate and 5'-OH ligase

| url = http://www.ncbi.nlm.nih.gov/gene/51493

| access-date = 2019-06-06

}} It is found in the stress granule of cells.{{cite journal | vauthors = Jain S, Wheeler JR, Walters RW, Agrawal A, Barsic A, Parker R | title = ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure | journal = Cell | volume = 164 | issue = 3 | pages = 487–98 | date = January 2016 | pmid = 26777405 | pmc = 4733397 | doi = 10.1016/j.cell.2015.12.038 }}

Structure

{{As of|2019|06}}, no crystal structure of the human RTCB is known, but homology models built from other RtcB-family ligases are available (Swiss-model: {{Swiss-model|Q9Y3I0}}). The structure of Pyrococcus horikoshii RtcB, which uses GTP instead of ATP, shows two manganese (Mn2+) cofactors, and a mechanism involving a covalently linked GTP-histidine-RtcB intermediate. The residue involved, H404, is conserved in human RTCB as H428.{{cite journal | vauthors = Desai KK, Bingman CA, Phillips GN, Raines RT | title = Structures of the noncanonical RNA ligase RtcB reveal the mechanism of histidine guanylylation | journal = Biochemistry | volume = 52 | issue = 15 | pages = 2518–25 | date = April 2013 | pmid = 23560983 | pmc = 3670941 | doi = 10.1021/bi4002375 }}

Function

{{Empty section|date=June 2019}}

Protein family

{{Infobox protein family

|Symbol=RtcB

|Name=tRNA splicing ligase RtcB

|InterPro=IPR001233

|Pfam=PF01139

|PROSITE=PS01288

|SCOP=1uc2

|CATH=1uc2

}}

RTCB belongs to the RtcB family of ATP-dependent RNA ligases, named after the eponymous protein in E. coli. The bacterial RtcB acts as a tRNA ligase, rejoining broken stem-loops in case of damage.{{cite journal | vauthors = Tanaka N, Shuman S | title = RtcB is the RNA ligase component of an Escherichia coli RNA repair operon | journal = The Journal of Biological Chemistry | volume = 286 | issue = 10 | pages = 7727–31 | date = March 2011 | pmid = 21224389 | doi = 10.1074/jbc.C111.219022 | pmc = 3048659 | doi-access = free }} It is also able to catalyse RNA splicing.{{cite journal | vauthors = Tanaka N, Meineke B, Shuman S | title = RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo | journal = The Journal of Biological Chemistry | volume = 286 | issue = 35 | pages = 30253–7 | date = September 2011 | pmid = 21757685 | pmc = 3162383 | doi = 10.1074/jbc.C111.274597 | doi-access = free }}

The eukaryotic homologs of RtcB, including the human RTCB protein, participates in the tRNA-splicing ligase complex.{{cite journal | vauthors = Popow J, Englert M, Weitzer S, Schleiffer A, Mierzwa B, Mechtler K, Trowitzsch S, Will CL, Lührmann R, Söll D, Martinez J | display-authors = 6 | title = HSPC117 is the essential subunit of a human tRNA splicing ligase complex | journal = Science | volume = 331 | issue = 6018 | pages = 760–4 | date = February 2011 | pmid = 21311021 | doi = 10.1126/science.1197847 | bibcode = 2011Sci...331..760P | hdl = 11858/00-001M-0000-0011-5682-B | s2cid = 1881494 | hdl-access = free }}

References

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Further reading

{{refbegin | 2}}

  • {{cite journal | vauthors = Pérez-González A, Pazo A, Navajas R, Ciordia S, Rodriguez-Frandsen A, Nieto A | title = hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex | journal = PLOS ONE | volume = 9 | issue = 3 | pages = e90957 | date = 2014 | pmid = 24608264 | pmc = 3946611 | doi = 10.1371/journal.pone.0090957 | bibcode = 2014PLoSO...990957P | doi-access = free }}
  • {{cite journal | vauthors = Ma H, Qi MY, Zhang X, Zhang YL, Wang L, Li ZQ, Fu B, Wang WT, Liu D | display-authors = 6 | title = HSPC117 is regulated by epigenetic modification and is involved in the migration of JEG-3 cells | journal = International Journal of Molecular Sciences | volume = 15 | issue = 6 | pages = 10936–49 | date = June 2014 | pmid = 24941254 | pmc = 4100190 | doi = 10.3390/ijms150610936 | doi-access = free }}
  • {{cite journal | vauthors = Jurkin J, Henkel T, Nielsen AF, Minnich M, Popow J, Kaufmann T, Heindl K, Hoffmann T, Busslinger M, Martinez J | display-authors = 6 | title = The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells | journal = The EMBO Journal | volume = 33 | issue = 24 | pages = 2922–36 | date = December 2014 | pmid = 25378478 | pmc = 4282640 | doi = 10.15252/embj.201490332 }}

{{refend}}

External links

  • MetaCyc: [https://biocyc.org/HUMAN/NEW-IMAGE?type=ENZYME&object=CPLX-9136 CPLX-9136]: Homo sapiens tRNA-splicing ligase complex ([http://amigo.geneontology.org/amigo/term/GO:0072669 GO:0072669])

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