Repression of heat shock gene expression (ROSE) element#ROSE1 and ROSEAT2

{{Infobox rfam

| Name = Repression of heat shock gene expression (ROSE) element

| image = RF00435.jpg

| width =

| caption = Predicted secondary structure and sequence conservation of ROSE

| Symbol = ROSE

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| Rfam = RF00435

| miRBase =

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| RNA_type = Cis-reg; thermoregulator

| Tax_domain = Bacteria

| GO =

| SO = {{SO|0000204}}

| CAS_number =

| EntrezGene =

| HGNCid =

| OMIM =

| PDB =

| RefSeq =

| Chromosome =

| Arm =

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}}

File:PDB 2gio EBI.png structure of the microROSE element.{{cite journal |doi=10.1038/sj.emboj.7601128 |vauthors=Chowdhury S, Maris C, Allain FH, Narberhaus F |title=Molecular basis for temperature sensing by an RNA thermometer |journal=EMBO J |volume=25 |issue=11 |pages=2487–2497 |year=2006 |pmid=16710302 |pmc=1478195}}]]

The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.{{cite journal | last = Nocker | first = A |author2=Hausherr T |author3=Balsiger S |author4=Krstulovic NP |author5=Hennecke H |author6=Narberhaus F | year = 2001 | title = A mRNA-based thermosensor controls expression of rhizobial heat shock genes | journal = Nucleic Acids Res | volume = 29 | pages = 4800–4807 | pmid = 11726689 | doi = 10.1093/nar/29.23.4800 | issue = 23 | pmc = 96696}}{{cite journal | last = Balsiger | first = S |author2=Ragaz C |author3=Baron C |author4=Narberhaus F | year = 2004 | title = Replicon-Specific Regulation of Small Heat Shock Genes in Agrobacterium tumefaciens | journal = J Bacteriol | volume = 186 | pages = 6824–6829 | pmid = 15466035 | doi = 10.1128/JB.186.20.6824-6829.2004 | issue = 20 | pmc = 522190}}

A partial structure of this RNA element has been determined using NMR.

ROSE<sub>1</sub> and ROSE<sub>AT2</sub>

ROSE₁ and ROSE_AT2 are specific examples of ROSE elements.{{cite journal |vauthors=Narberhaus F, Waldminghaus T, Chowdhury S |title=RNA thermometers |journal=FEMS Microbiol. Rev. |volume=30 |issue=1 |pages=3–16 |date=January 2006 |pmid=16438677 |doi=10.1111/j.1574-6976.2005.004.x |doi-access= }} ROSE₁ is found in Bradyrhizobium japonicum whereas ROSE_AT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE₁ having an extra hairpin. All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.{{cite journal |vauthors=Nocker A, Hausherr T, Balsiger S, Krstulovic NP, Hennecke H, Narberhaus F |title=A mRNA-based thermosensor controls expression of rhizobial heat shock genes |journal=Nucleic Acids Res. |volume=29 |issue=23 |pages=4800–4807 |date=December 2001 |pmid=11726689 |pmc=96696 |doi= 10.1093/nar/29.23.4800|url=}}

IpbA

Expression of the heat shock protein IpbA in species of Pseudomonas is controlled by a ROSE-type RNA thermometer. This thermometer only consists of two hairpins. It inhibits translation of IpbA at low temperatures and permits translation when temperature increases.{{cite journal|last=Krajewski|first=SS|author2=Nagel, M |author3=Narberhaus, F |title=Short ROSE-Like RNA Thermometers Control IbpA Synthesis in Pseudomonas Species.|journal=PLOS ONE|date=2013|volume=8|issue=5|pages=e65168|pmid=23741480|doi=10.1371/journal.pone.0065168|pmc=3669281|bibcode=2013PLoSO...865168K|doi-access=free}}

''rhlA'' and ''lsdI'' ROSE-like elements

Pseudomonas aeruginosa quorum sensing-dependent virulence factors can be thermoregulated. ROSE thermometer present in the 5'UTR of rhlA and ROSE-like thermometer in the 5'UTR of lasI block the translation at lower temperatures.{{Cite journal|last1=Grosso-Becerra|first1=María Victoria|last2=Croda-García|first2=Gerardo|last3=Merino|first3=Enrique|last4=Servín-González|first4=Luis|last5=Mojica-Espinosa|first5=Raúl|last6=Soberón-Chávez|first6=Gloria|date=2014-10-28|title=Regulation of Pseudomonas aeruginosa virulence factors by two novel RNA thermometers|journal=Proceedings of the National Academy of Sciences of the United States of America|volume=111|issue=43|pages=15562–15567|doi=10.1073/pnas.1402536111|issn=1091-6490|pmc=4217398|pmid=25313031|bibcode=2014PNAS..11115562G|doi-access=free}}

References

External links

{{Rfam|id=RF00435|name=Repression of heat shock gene expression (ROSE) element}}

{{DEFAULTSORT:Repression Of Heat Shock Gene Expression (Rose) Element}}

Category:Cis-regulatory RNA elements