SF3A2

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

SF3A2 has been shown to interact with DDX46.{{cite journal | vauthors = Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R | title = Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein | journal = The EMBO Journal | volume = 21 | issue = 18 | pages = 4978–88 | date = September 2002 | pmid = 12234937 | pmc = 126279 | doi = 10.1093/emboj/cdf480 }}

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• {{cite journal | vauthors = Chiara MD, Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R | title = Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114 | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 91 | issue = 14 | pages = 6403–7 | date = July 1994 | pmid = 8022796 | pmc = 44210 | doi = 10.1073/pnas.91.14.6403 | bibcode = 1994PNAS...91.6403C | doi-access = free }}
• {{cite journal | vauthors = Rain JC, Tartakoff AM, Krämer A, Legrain P | title = Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution | journal = RNA | volume = 2 | issue = 6 | pages = 535–50 | date = June 1996 | pmid = 8718683 | pmc = 1369393 }}
• {{cite journal | vauthors = Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R | title = Association of U2 snRNP with the spliceosomal complex E | journal = Nucleic Acids Research | volume = 25 | issue = 2 | pages = 354–61 | date = January 1997 | pmid = 9016565 | pmc = 146436 | doi = 10.1093/nar/25.2.354 }}
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• {{cite journal | vauthors = Yan D, Perriman R, Igel H, Howe KJ, Neville M, Ares M | title = CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif | journal = Molecular and Cellular Biology | volume = 18 | issue = 9 | pages = 5000–9 | date = September 1998 | pmid = 9710584 | pmc = 109085 | doi = 10.1128/mcb.18.9.5000}}
• {{cite journal | vauthors = Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M | title = Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex | journal = Nature Genetics | volume = 20 | issue = 1 | pages = 46–50 | date = September 1998 | pmid = 9731529 | doi = 10.1038/1700 | s2cid = 585778 }}
• {{cite journal | vauthors = Krämer A, Grüter P, Gröning K, Kastner B | title = Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP | journal = The Journal of Cell Biology | volume = 145 | issue = 7 | pages = 1355–68 | date = June 1999 | pmid = 10385517 | pmc = 2133165 | doi = 10.1083/jcb.145.7.1355 }}
• {{cite journal | vauthors = Das R, Zhou Z, Reed R | title = Functional association of U2 snRNP with the ATP-independent spliceosomal complex E | journal = Molecular Cell | volume = 5 | issue = 5 | pages = 779–87 | date = May 2000 | pmid = 10882114 | doi = 10.1016/S1097-2765(00)80318-4 | doi-access = free }}
• {{cite journal | vauthors = Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC | title = A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site | journal = The EMBO Journal | volume = 20 | issue = 16 | pages = 4536–46 | date = August 2001 | pmid = 11500380 | pmc = 125580 | doi = 10.1093/emboj/20.16.4536 }}
• {{cite journal | vauthors = Nesic D, Krämer A | title = Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation | journal = Molecular and Cellular Biology | volume = 21 | issue = 19 | pages = 6406–17 | date = October 2001 | pmid = 11533230 | pmc = 99788 | doi = 10.1128/MCB.21.19.6406-6417.2001 }}
• {{cite journal | vauthors = Dresser DW, Jamin SP, Atkins CJ, Guerrier D | title = An expressed GNRP-like gene shares a bi-directional promoter with SF3A2 (SAP62) immediately upstream of AMH | journal = Gene | volume = 277 | issue = 1–2 | pages = 163–73 | date = October 2001 | pmid = 11602354 | doi = 10.1016/S0378-1119(01)00690-4 }}
• {{cite journal | vauthors = Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ | title = Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis | journal = RNA | volume = 8 | issue = 4 | pages = 426–39 | date = April 2002 | pmid = 11991638 | pmc = 1370266 | doi = 10.1017/S1355838202021088 }}
• {{cite journal | vauthors = Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R | title = Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein | journal = The EMBO Journal | volume = 21 | issue = 18 | pages = 4978–88 | date = September 2002 | pmid = 12234937 | pmc = 126279 | doi = 10.1093/emboj/cdf480 }}
• {{cite journal | vauthors = Takenaka K, Nakagawa H, Miyamoto S, Miki H | title = The pre-mRNA-splicing factor SF3a66 functions as a microtubule-binding and -bundling protein | journal = The Biochemical Journal | volume = 382 | issue = Pt 1 | pages = 223–30 | date = August 2004 | pmid = 15142036 | pmc = 1133934 | doi = 10.1042/BJ20040521 }}
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• {{cite journal | vauthors = Nesic D, Tanackovic G, Krämer A | title = A role for Cajal bodies in the final steps of U2 snRNP biogenesis | journal = Journal of Cell Science | volume = 117 | issue = Pt 19 | pages = 4423–33 | date = September 2004 | pmid = 15316075 | doi = 10.1242/jcs.01308 | url = https://archive-ouverte.unige.ch/unige:17542/ATTACHMENT01 | doi-access = free }}

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