Sabouraud agar

{{Short description|Agar media containing peptones}}

Image:Trichophyton rubrum var. rodhaini PHIL 4248 lores.jpg var. rodhaini]]

Image:Sporothrix schenckii PHIL 3943 lores.jpg in Sabouraud agar]]

Image:Candida albicans PHIL 3192 lores.jpg in Sabouraud agar]]

Image:Trichophyton terrestre PHIL 4300 lores.jpg terrestre in Sabouraud agar]]

Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones.{{cite web |url=http://www.waksmanfoundation.org/labs/lsu/omni.html |title=Omnipresence of Microorganisms in the Environment | work = Waksman Foundation For Microbiology |access-date=2008-10-24 |url-status=dead |archive-url=https://web.archive.org/web/20081006012923/http://waksmanfoundation.org/labs/lsu/omni.html |archive-date=2008-10-06 }} It is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as Nocardia.{{cite journal | vauthors = Sandven P, Lassen J | title = Importance of selective media for recovery of yeasts from clinical specimens | journal = Journal of Clinical Microbiology | volume = 37 | issue = 11 | pages = 3731–3732 | date = November 1999 | pmid = 10523586 | pmc = 85742 | doi = 10.1128/JCM.37.11.3731-3732.1999 }}{{cite journal | vauthors = Guinea J, Peláez T, Alcalá L, Bouza E | title = Evaluation of Czapeck agar and Sabouraud dextrose agar for the culture of airborne Aspergillus conidia | journal = Diagnostic Microbiology and Infectious Disease | volume = 53 | issue = 4 | pages = 333–334 | date = December 2005 | pmid = 16263232 | doi = 10.1016/j.diagmicrobio.2005.07.002 }}{{Cite web|url= http://www.bd.com/ds/technicalCenter/inserts/Sabouraud_Agar_Modified.pdf | archive-url = https://web.archive.org/web/20090419083512/http://www.bd.com/ds/technicalCenter/inserts/Sabouraud_Agar_Modified.pdf | archive-date = 19 April 2009 |title= About Modified Sabouraud Agar |website=www.bd.com}} It has utility for research and clinical care.

It was created by, and is named after, Raymond Sabouraud in 1892. In 1977 the formulation was adjusted by Chester W. Emmons when the pH level was brought closer to the neutral range and the dextrose concentration lowered to support the growth of other microorganisms. The acidic pH (5.6) of traditional Sabouraud agar inhibits bacterial growth.{{cite book | vauthors = Hare JM | veditors = Gupta VK, Tuohy MG, Ayyachamy M, Turner KM, O'Donovan A |title=Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology |date=9 December 2012 |publisher=Springer |isbn=978-1-4614-2355-3 |page=212 |chapter-url=https://books.google.com/books?id=IQOwjVXwEQIC&pg=PA212 |language=en |chapter=15. Sabouraud agar for fungal growth}} Peptones are complex digests and can be a source of variability in Sabouraud agar.{{cite journal | vauthors = Odds FC | title = Sabouraud('s) agar | journal = Journal of Medical and Veterinary Mycology | volume = 29 | issue = 6 | pages = 355–359 | date = January 1991 | pmid = 1815027 | doi = 10.1080/02681219180000581 }}

Typical composition

Sabouraud agar is commercially available and typically contains:{{cite web | work = University of Sydney | url = http://bugs.bio.usyd.edu.au/learning/resources/Mycology/Growth_Dev/recipes.shtml#sabouraud | archive-url = https://web.archive.org/web/20121214202207/http://bugs.bio.usyd.edu.au/learning/resources/Mycology/Growth_Dev/recipes.shtml | archive-date = 14 December 2012 | date = 2004 | title = Recipes }}

Medical use

Clinical laboratories can use this growth medium to diagnose and further speciate fungal infections, allowing medical professionals to provide appropriate treatment with antifungal medications. Histoplasma and other fungal causes of atypical pneumonia can be grown on this medium. Sabouraud agar used in combination with additional media, such as Inhibitory Mold Agar (IMA), improves identification of fungal clinical isolates.{{cite journal | vauthors = Scognamiglio T, Zinchuk R, Gumpeni P, Larone DH | title = Comparison of inhibitory mold agar to Sabouraud dextrose agar as a primary medium for isolation of fungi | journal = Journal of Clinical Microbiology | volume = 48 | issue = 5 | pages = 1924–1925 | date = May 2010 | pmid = 20220162 | pmc = 2863941 | doi = 10.1128/JCM.01814-09 }}

References

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{{Growth media}}

Category:Microbiological media

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