Sf9 (cells)
{{short description|Insect cell line}}
File:SF9 Cells phase contrast Lara Rudman.jpg
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue.{{cite journal |author1=Vaughn JL |author2=Goodwin RH |author3=Tompkins GJ |author4=McCawley P | title=The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae) | journal=In Vitro | year=1977 | volume=13 | issue=4 | pages=213–217 | pmid= 68913 | doi=10.1007/BF02615077|s2cid=13529129 }} They can be grown in the absence of serum, and can be cultured attached or in suspension.{{cite book | author=Invitrogen| title=Growth and Maintenance of Insect cell lines: User Guide | section=Cell Lines | version=Rev. Date - 8 March 2010 | publisher=Invitrogen | date=14 Mar 2017 | url=https://tools.thermofisher.com/content/sfs/manuals/Insect_Cell_Lines_UG.pdf | id=Manual part no. 25-0127, MAN0000030 |accessdate=12 May 2017 }}
Sf9 Rhabdovirus
It has previously been shown that some Sf9 cell lines harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV).{{cite journal| author=Ma H, Galvin TA, Glasner DR, Shaheduzzaman S, Khan AS| title=Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines. | journal=J Virol | year= 2014 | volume= 88 | issue= 12 | pages= 6576–85 | pmid=24672045 | doi=10.1128/JVI.00780-14 | pmc=4054387 }} {{cite journal| author=Parry R, de Malmanche H, Asgari S| title=Persistent Spodoptera frugiperda rhabdovirus infection in Sf9 cells is not restricted by Wolbachia wMelPop-CLA and wAlbB strains and is targeted by the RNAi machinery. | journal=Virology | year= 2021 | volume= 563 | issue= | pages= 82–87 | pmid=34492433 | doi=10.1016/j.virol.2021.08.013 | pmc= | doi-access=free }} However, not all tested Sf9 cells appear to be infected with this virus.{{cite journal| author=Hashimoto Y, Macri D, Srivastava I, McPherson C, Felberbaum R, Post P | display-authors=etal| title=Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line. | journal=PLOS ONE | year= 2017 | volume= 12 | issue= 4 | pages= e0175633 | pmid=28423032 | doi=10.1371/journal.pone.0175633 | pmc=5397025 | bibcode=2017PLoSO..1275633H| doi-access=free}} SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.{{cite journal| author=Maghodia AB, Jarvis DL| title=Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines. | journal=Virology | year= 2017 | volume= 512 | issue= | pages= 234–245 | pmid=29024851 | doi=10.1016/j.virol.2017.09.025 | pmc=5651996 }}
References
External links
- [https://www.cellosaurus.org/CVCL_0549 Cellosaurus entry for Sf9]