Solid phase sequencing

The principle of solid phase DNA sequencing was described in 1989 based on binding of biotinylated DNA to streptavidin-coated magnetic beads and elution of single DNA strands selectively using alkali.{{cite journal |last1=Hultman |first1=T |last2=Ståhl |first2=S |last3=Hornes |first3=E |last4=Uhlén |first4=M |title=Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. |journal=Nucleic Acids Research |date=11 July 1989 |volume=17 |issue=13 |pages=4937–4946 |pmc=318085 |pmid=2668874 |doi=10.1093/nar/17.13.4937}} The method allowed robotic applications suitable for clinical sequencing, but the magnetic handling has also found frequent use in many molecular applications, including sample handling for DNA diagnostics.{{cite journal |last1=Uhlen |first1=M. |title=Magnetic separation of DNA |journal=Nature |date=August 1989 |volume=340 |issue=6236 |pages=733–734 |doi=10.1038/340733a0 |pmid=2770876 |bibcode=1989Natur.340..733U |s2cid=4319266 }} The use of solid phase methods for DNA handling is now frequently used as an integrated part of many of the next generation DNA sequencing methods, as well as numerous molecular diagnostics applications.{{fact|date=February 2020}}