TRNA pseudouridine32 synthase

{{Infobox enzyme

| Name = tRNA pseudouridine³² synthase

| EC_number = 5.4.99.28

| CAS_number = 430429-15-5

| GO_code =

| image =

| width =

| caption =

}}

tRNA pseudouridine³² synthase ({{EnzExplorer|5.4.99.28}}, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine³² uracil mutase.{{cite journal | vauthors = Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferré-D'Amaré AR | title = Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure | journal = Molecular Cell | volume = 24 | issue = 4 | pages = 535–45 | date = November 2006 | pmid = 17188032 | doi = 10.1016/j.molcel.2006.09.017 | doi-access = free }}{{cite journal | vauthors = Spedaliere CJ, Hamilton CS, Mueller EG | title = Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues | journal = Biochemistry | volume = 39 | issue = 31 | pages = 9459–65 | date = August 2000 | pmid = 10924141 | doi = 10.1021/bi001079n }}{{cite journal | vauthors = Raychaudhuri S, Niu L, Conrad J, Lane BG, Ofengand J | title = Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA | journal = The Journal of Biological Chemistry | volume = 274 | issue = 27 | pages = 18880–6 | date = July 1999 | pmid = 10383384 | doi = 10.1074/jbc.274.27.18880 | doi-access = free }}{{cite journal | vauthors = Ramamurthy V, Swann SL, Spedaliere CJ, Mueller EG | title = Role of cysteine residues in pseudouridine synthases of different families | journal = Biochemistry | volume = 38 | issue = 40 | pages = 13106–11 | date = October 1999 | pmid = 10529181 | doi = 10.1021/bi9913911 }}{{cite journal | vauthors = Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J | title = A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe) | journal = RNA | volume = 1 | issue = 4 | pages = 437–48 | date = June 1995 | pmid = 7493321 | pmc = 1482406 }}{{cite journal | vauthors = Behm-Ansmant I, Grosjean H, Massenet S, Motorin Y, Branlant C | title = Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae | journal = The Journal of Biological Chemistry | volume = 279 | issue = 51 | pages = 52998–3006 | date = December 2004 | pmid = 15466869 | doi = 10.1074/jbc.m409581200 | s2cid = 13197875 | url = https://hal.univ-lorraine.fr/hal-01738505/file/Behm-Ansmant%20et%20al.%2C%202004%2C%20JBC%2C%20Pus8_9.pdf | doi-access = free }} This enzyme catalyses the following chemical reaction

: tRNA uridine³² $\backslash rightleftharpoons$ tRNA pseudouridine³²

The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine⁷⁴⁶ in 23S rRNA.