Thiocyanate hydrolase
{{infobox enzyme
| Name = thiocyanate hydrolase
| EC_number = 3.5.5.8
| CAS_number =
| GO_code = 0018760
| image =
| width =
| caption =
}}
A thiocyanate hydrolase ({{EC number|3.5.5.8}}) is an enzyme belonging to the family of hydrolases. The systematic name of this enzyme class is thiocyanate aminohydrolase.
This enzyme catalyzes the chemical reaction:
:{{chem2|SCN- + 2 H2O + H+ <-> SCO + NH3}}
The mechanism is proposed to involve a metal thiocyanate complex.
Structural studies
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes {{PDB link|2DD4}}, {{PDB link|2DD5}}, {{PDB link|2DXB}}, and {{PDB link|2DXC}}.
A second thiocyanate hydrolase with copper at its active site catalyzes its conversion to cyanate:{{cite journal|doi=10.1073/pnas.1922133117 |title=Trinuclear Copper Biocatalytic Center Forms an Active Site of Thiocyanate Dehydrogenase |date=2020 |last1=Tikhonova |first1=Tamara V. |last2=Sorokin |first2=Dimitry Y. |last3=Hagen |first3=Wilfred R. |last4=Khrenova |first4=Maria G. |last5=Muyzer |first5=Gerard |last6=Rakitina |first6=Tatiana V. |last7=Shabalin |first7=Ivan G. |last8=Trofimov |first8=Anton A. |last9=Tsallagov |first9=Stanislav I. |last10=Popov |first10=Vladimir O. |journal=Proceedings of the National Academy of Sciences |volume=117 |issue=10 |pages=5280–5290 |doi-access=free |pmid=32094184 |bibcode=2020PNAS..117.5280T |pmc=7071890 }}
:{{chem2|SCN- + H2O -> OCN- + H2S}}
References
{{reflist|1}}
- {{cite journal | vauthors = Katayama Y, Matsushita Y, Kaneko M, Kondo M, Mizuno T, Nyunoya H | date = 1998 | title = Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase | journal = J. Bacteriol. | volume = 180 | pages = 2583–9 | pmid = 9573140 | issue = 10 | doi = 10.1128/JB.180.10.2583-2589.1998 | pmc = 107207 }}
- {{cite journal | vauthors = Katayama Y, Narahara Y, Inoue Y, Amano F, Kanagawa T, Kuraishi H | date = 1992 | title = A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate | journal = J. Biol. Chem. | volume = 267 | pages = 9170–5 | pmid = 1577754 | issue = 13 | doi = 10.1016/S0021-9258(19)50404-5 | doi-access = free }}
{{Carbon-nitrogen non-peptide hydrolases}}
{{Enzymes}}
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Category:Enzymes of known structure
{{3.5-enzyme-stub}}