Two Brothers

• Two Brothers (1929 film), a German silent film directed by Mikhail Dubson
• Two Brothers (2004 film), a French-British film directed by Jean-Jacques Annaud
• The Two Brothers (film), a 1910 American short Western film directed by D. W. Griffith
• Do Bhai (disambiguation) ({{lit|Two Brothers}}), various Indian films
• Two Brothers Pictures, a British television production company founded in 2014

• Tale of Two Brothers, an ancient Egyptian story
• The Two Brothers, a German fairy tale
• The Two Brothers (novel), or La Rabouilleuse, an 1842 novel by Honoré de Balzac
• Dois Irmãos (Two Brothers), a 2000 novel by Milton Hatoum

• "The Twa Brothers", a traditional ballad
• Two Brothers (album), a 2001 album by Boxhead Ensemble

• Two Brothers, U.S. Virgin Islands, a settlement on the island of Saint Croix
• Two Brothers Rocks, a rock formation in the Ionian Sea near Syracuse, Sicily

• Two Brothers (ship), a 19th-century Nantucket whaleship
• Two Brothers (video game), a 2013 action role-playing game
• Two Brothers Brewing, an American microbrewery
• Two Brothers Roundhouse, on the former Chicago & Aurora Railroad, in Aurora, Illinois, U.S.

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