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User:Rockpocket/MGP/NFKB1

==Model organisms==

Model organisms have been used in the study of NFKB1 function. A conditional knockout mouse line, called Nfkb1tm1a(KOMP)Wtsi{{cite web |url=http://www.knockoutmouse.org/martsearch/search?query=Nfkb1 |title=International Knockout Mouse Consortium}}{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4363738 |title=Mouse Genome Informatics}} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.{{Cite journal| last1 = Skarnes |first1 =W. C.| doi = 10.1038/nature10163 | last2 = Rosen | first2 = B.| last3 = West | first3 = A. P.| last4 = Koutsourakis | first4 = M.| last5 = Bushell | first5 = W.| last6 = Iyer | first6 = V.| last7 = Mujica | first7 = A. O.| last8 = Thomas | first8 = M.| last9 = Harrow | first9 = J.| last10 = Cox | first10 = T.| last11 = Jackson | first11 = D.| last12 = Severin | first12 = J.| last13 = Biggs | first13 = P.| last14 = Fu | first14 = J.| last15 = Nefedov | first15 = M.| last16 = De Jong | first16 = P. J.| last17 = Stewart | first17 = A. F.| last18 = Bradley | first18 = A. | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–342 | year = 2011 | pmid = 21677750 | pmc =3572410 }}{{cite web |url=http://www.nature.com/news/2011/110615/full/474262a.html |title=Mouse library set to be knockout |author=Dolgin E |year=June 2011 |location=Nature 474: 262-263. doi:10.1038/474262a}}{{cite book |title=A mouse for all reasons |author=Collins FS, Rossant J, Wurst W |year=January 2007 |location=Cell 128(1): 9-13. doi:10.1016/j.cell.2006.12.018 PMID 17218247}}

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|+ Nfkb1 knockout mouse phenotype

CharacteristicPhenotype
Homozygote viabilitybgcolor="#488ED3"|Normal
Fertilitybgcolor="#488ED3"|Normal
Body weightbgcolor="#488ED3"|Normal
Anxietybgcolor="#488ED3"|Normal
Neurological assessmentbgcolor="#488ED3"|Normal
Grip strengthbgcolor="#488ED3"|Normal
Hot platebgcolor="#488ED3"|Normal
Dysmorphologybgcolor="#488ED3"|Normal
Indirect calorimetrybgcolor="#C40000"|Abnormal
Glucose tolerance testbgcolor="#488ED3"|Normal
Auditory brainstem responsebgcolor="#488ED3"|Normal
DEXAbgcolor="#488ED3"|Normal
Radiographybgcolor="#488ED3"|Normal
Body temperaturebgcolor="#488ED3"|Normal
Eye morphologybgcolor="#488ED3"|Normal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAHS/eye-morphology/ |title=Eye morphology data for Nfkb1 |publisher=Wellcome Trust Sanger Institute}}
Clinical chemistrybgcolor="#C40000"|Abnormal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAHS/plasma-chemistry/ |title=Clinical chemistry data for Nfkb1 |publisher=Wellcome Trust Sanger Institute}}
Plasma immunoglobulinsbgcolor="#C40000"|Abnormal
Haematologybgcolor="#C40000"|Abnormal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAHS/haematology-cbc/ |title=Haematology data for Nfkb1 |publisher=Wellcome Trust Sanger Institute}}
Peripheral blood lymphocytesbgcolor="#C40000"|Abnormal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAHS/peripheral-blood-lymphocytes/ |title=Peripheral blood lymphocytes data for Nfkb1 |publisher=Wellcome Trust Sanger Institute}}
Micronucleus testbgcolor="#488ED3"|Normal
Heart weightbgcolor="#488ED3"|Normal
Tail epidermis wholemountbgcolor="#488ED3"|Normal
Skin Histopathologybgcolor="#488ED3"|Normal
Brain histopathologybgcolor="#488ED3"|Normal
Salmonella infectionbgcolor="#C40000"|Abnormal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAHS/salmonella-challenge/ |title=Salmonella infection data for Nfkb1 |publisher=Wellcome Trust Sanger Institute}}
colspan=2; style="text-align: center;" | All tests and analysis from{{cite web |url=http://onlinelibrary.wiley.com/doi/10.1111/j.1755-3768.2010.4142.x/abstract |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |location=Acta Opthalmologica 88: 925-7.doi:10.1111/j.1755-3768.2010.4142.x |publisher=Wiley}}

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.{{cite journal| author=van der Weyden L, White JK, Adams DJ, Logan DW| title=The mouse genetics toolkit: revealing function and mechanism. | journal=Genome Biol | year= 2011 | volume= 12 | issue= 6 | pages= 224 | pmid=21722353 | doi=10.1186/gb-2011-12-6-224 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=21722353 }} Twenty five tests were carried out on mutant mice and six significant abnormalities were observed. Female homozygotes had a decreased respiratory quotient, increased circulating alkaline phosphatase level and increased leukocyte cell number. Male homozygotes showed an increased susceptibility to Salmonella infection, while homozygotes of both sex had decreased IgG1 and decreased regulatory T cell and NK cell numbers.

References

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