Vts1
{{technical|date=August 2016}}
{{Infobox protein family
| Symbol = Vts1
| Name = Vts1
| image = Vts1P_SRE_Recognition_SAM_domain.jpg
| width =
| caption = Crystal structure of Vts1p–SRE complex.{{cite journal | vauthors = Johnson PE, Donaldson LW | title = RNA recognition by the Vts1p SAM domain | journal = Nature Structural & Molecular Biology | volume = 13 | issue = 2 | pages = 177–178 | date = February 2006 | pmid = 16429155 | doi = 10.1038/nsmb1039 | s2cid = 9561521 }}
| Pfam = PF07647
| Pfam_clan =
| InterPro =
| SMART =
| PROSITE =
| MEROPS =
| SCOP = 1b0x
| TCDB =
| OPM family =
| OPM protein =
| CAZy =
| CDD =
}}
Vts1 is a post-transcriptional regulator that has RNA-binding Sterile alpha motif (SAM) domain.{{cite journal | vauthors = Schultz J, Ponting CP, Hofmann K, Bork P | title = SAM as a protein interaction domain involved in developmental regulation | journal = Protein Science | volume = 6 | issue = 1 | pages = 249–253 | date = January 1997 | pmid = 9007998 | pmc = 2143507 | doi = 10.1002/pro.5560060128 }}{{cite journal | vauthors = Oberstrass FC, Lee A, Stefl R, Janis M, Chanfreau G, Allain FH | title = Shape-specific recognition in the structure of the Vts1p SAM domain with RNA | journal = Nature Structural & Molecular Biology | volume = 13 | issue = 2 | pages = 160–167 | date = February 2006 | pmid = 16429156 | doi = 10.1038/nsmb1038 | s2cid = 15416866 }} The protein is found in Saccharomyces cerevisiae and several eukaryotes. In Saccharomyces the Vts1 impacts vesicular transport and sporulation.{{cite journal | vauthors = Deutschbauer AM, Williams RM, Chu AM, Davis RW | title = Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 99 | issue = 24 | pages = 15530–15535 | date = November 2002 | pmid = 12432101 | pmc = 137751 | doi = 10.1073/pnas.202604399 | doi-access = free | bibcode = 2002PNAS...9915530D }}{{cite journal | vauthors = Dilcher M, Köhler B, von Mollard GF | title = Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6 | journal = The Journal of Biological Chemistry | volume = 276 | issue = 37 | pages = 34537–34544 | date = September 2001 | pmid = 11445562 | doi = 10.1074/jbc.M101551200 | doi-access = free }}
Interactions
Protein-protein interactions through SAM domains participate in different regulatory activities such as signal transduction. Proteins having such domains were also shown to recognize and interact with RNA structures of similar shape to the Smaug response element (SRE).{{cite journal | vauthors = Hall TM | title = SAM breaks its stereotype | journal = Nature Structural Biology | volume = 10 | issue = 9 | pages = 677–679 | date = September 2003 | pmid = 12942139 | doi = 10.1038/nsb0903-677 | s2cid = 9072100 | url = https://zenodo.org/record/1233554 }} Vts1 binds to RNA targets that have CUGGC on hairpin loops.{{cite journal | vauthors = Oberstrass FC, Lee A, Stefl R, Janis M, Chanfreau G, Allain FH | title = Shape-specific recognition in the structure of the Vts1p SAM domain with RNA | journal = Nature Structural & Molecular Biology | volume = 13 | issue = 2 | pages = 160–167 | date = February 2006 | pmid = 16429156 | doi = 10.1038/nsmb1038 | s2cid = 15416866 }}