Whey protein

{{Main article|Whey}}

Whey is left over when milk is coagulated during the process of cheese production. Coagulation can happen by adding acid or rennet. It is a 5% solution of lactose in water and contains the water-soluble proteins of milk as well as some lipid content."Whey." The Encyclopædia Britannica. 15th ed. 1994 Processing can be done by simple drying, or the relative protein content can be increased by removing the lactose, lipids and other non-protein materials.{{Cite journal | title = Advances in modifying and understanding whey protein functionality | year = 2002 | journal = Trends in Food Science & Technology | pages = 151–159

| volume = 13 | issue = 5 | last1 =Foegeding | first1 =EA | last2 =Davis | first2 =JP | last3 =Doucet | first3 =D | last4 =McGuffey | first4 =MK | doi=10.1016/S0924-2244(02)00111-5

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The main method to extract protein from whey is membrane filtration. A variety of membrane pore sizes can be used to selectively let different components of whey go through or be retained. Whey can be passed through "microfiltration" which blocks bacteria, casein micelles, and fat, then passed through "ultrafiltration" (UF) which blocks proteins. The part that does not go through UF is spray-dried into a concentrated whey protein.{{cite book |author=Tunick MH |year=2008 |chapter=Whey Protein Production and Utilization. |veditors=Onwulata CI, Huth PJ |title=Whey processing, functionality and health benefits |place=Ames, Iowa |publisher=Blackwell Publishing; IFT Press |pages=1–13 |chapter-url=http://www.ars.usda.gov/research/publications/Publications.htm?seq_no_115=209388 |chapter-format=abstract}} There are also other ways to concentrate protein using filtration membranes.{{cite journal |last1=Buchanan |first1=Dominic |last2=Martindale |first2=Wayne |last3=Romeih |first3=Ehab |last4=Hebishy |first4=Essam |title=Recent advances in whey processing and valorisation: Technological and environmental perspectives |journal=International Journal of Dairy Technology |date=May 2023 |volume=76 |issue=2 |pages=291–312 |doi=10.1111/1471-0307.12935|doi-access=free }}

Ion exchange chromatography is another major method for whey protein extraction. Methods in development include aqueous two-phase extraction and magnetic fishing.

File:Modern Kitchen strawberry cream cheese 2.jpg, made with non-animal whey protein produced by microbes]]

Microbes have been engineered to produce proteins similar or even "bioidentical" to whey.{{cite news | newspaper=LA Times | author = Lerissa Zimberoff | date=July 11, 2019 | title=Here comes lab-grown dairy: milk proteins made without animals | url=https://www.latimes.com/business/la-fi-lab-dairy-synthetic-whey-protein-gmo-20190711-story.html}} Various companies offer microbe-produced whey and cheese; however, none of these companies stipulate the protein composition of their products, but they do contain some of the genes needed to make whey proteins.{{cite web |title=California Performance vegan whey |url=https://californiaperformance.co/products/v-whey-protein-powder-vanilla-sundae-flavor |access-date=May 3, 2022}}{{cite patent | title=Food compositions comprising one or both of recombinant beta-lactoglobulin protein and recombinant alpha-lactalbumin protein | number=US9924728B2 | pridate= August 21, 2014 |pubdate= March 27, 2018 | inventor= Ryan Pandya, Perumal Gandhi, Shaowen Ji, Derek Beauchamp, Louis Hom| assign1=Perfect Day Inc. }}

{{Main article|Protein quality}}

The protein in cow's milk is approximately 20% whey and 80% casein.{{Cite journal|author1=Jay R. Hoffman |author2=Michael J. Falvo |name-list-style=amp |journal= Journal of Sports Science and Medicine |year=2004 |issue=3 |pages=118–130 |title=Protein - Which is best?}} The protein in human milk is approximately 60% whey and 40% casein.{{cite journal |vauthors=Luhovyy BL, Akhavan T, Anderson GH | title=Whey proteins in the regulation of food intake and satiety | journal=Journal of the American College of Nutrition | volume=26 | issue=6 | year=2007 | pages=704S–712S | url = http://www.jacn.org/cgi/content/full/26/6/704S | pmid=18187437 | doi=10.1080/07315724.2007.10719651| s2cid=25573353 | url-access=subscription }} The protein fraction in whey constitutes approximately 10% of the total dry solids in whey. This protein is typically a mixture of beta-lactoglobulin (~65%), alpha-lactalbumin (~25%), bovine serum albumin (~8%) (see also serum albumin), and immunoglobulins.{{cite journal |last1=Haug |first1=A |last2=Høstmark |first2=AT |last3=Harstad |first3=OM|date=2007|title=Bovine milk in human nutrition – a review |journal=Lipids Health Dis |volume=6 |page=25 | doi=10.1186/1476-511X-6-25 |pmid=17894873 |pmc=2039733 |doi-access=free }} The third largest fragment of whey protein isolate derived from sweet whey is glycomacropeptide or GMP. However, GMP lacks the secondary structure necessary for it to be classified as a protein and is considered a long amino acid chain. These peptides are all soluble in water in their native forms.

Four major types of whey protein are produced commercially:{{cite web |last1=Rawat |first1=Manas |title=Types Of Whey Protein And Which One Is The Best For You |url=https://shreddedyouth.com/types-of-whey-protein/ |website=shreddedyouth |publisher=Manas Rawat |access-date=17 September 2022}}

• Whey Protein Concentrates (WPC) have typically low - though not absent - levels of fat and cholesterol. They also contain carbohydrates in the form of lactose. Powders are typically between 29%–89% protein by dry weight.
• Whey Protein Isolates (WPI) are processed to remove fat and lactose, and as a result, WPI powders are typically over 90% protein by dry weight. Like WPC, WPI are mild and slightly milky in taste.
• Whey Protein Hydrolysates (WPH) are whey proteins that are predigested and partially hydrolyzed for the purpose of easier metabolizing. Their cost is generally higher than WPC and WPI. Highly hydrolysed whey may be less allergenic than other forms of whey, due to its smaller peptide chains. For this reason it is a common constituent in hypoallergenic baby milk formulas and medical foods.{{cite journal |author=Lee YH |title=Food-processing approaches to altering allergenic potential of milk-based formula. |journal=J. Pediatr. |volume=121 |issue=5 Pt 2 |pages=S47–50 |date=November 1992 |pmid=1447634 |doi=10.1016/S0022-3476(05)81406-4}}
• Native whey protein is extracted from skim milk, rather than being collected as a byproduct of cheese production. This type of whey does not contain glycomacropeptide, which is formed only after the addition of rennet.{{cite web |url=http://www.dairyaustralia.com.au/~/media/341F6B4315484280B47F88504BCFB42F.pdf |title=Technologies for whey processing: "Is there a better whey?" |author1=Alan L. Kelly |author2=Seamus A. O'Mahony |website=Dairyaustralia.com.au |access-date=2016-05-19 |archive-date=30 March 2015 |archive-url=https://web.archive.org/web/20150330025602/http://www.dairyaustralia.com.au/~/media/341F6B4315484280B47F88504BCFB42F.pdf |url-status=dead }}{{cite web|url=http://www.usdairy.com/~/media/usd/public/technicalreportmilkfractionationtechnology.pdf.pdf|title=Technical Report: Milk Fractionation Technology and Emerging Milk Protein Opportunities|last=Burrington|first=Kimberlee|website=USDairy|publisher=U.S. Dairy Export Council|access-date=23 May 2016}}{{Cite web|url=http://www.denverpost.com/2016/05/23/leprino-foods-enters-direct-to-consumer-whey-protein-market-with-ascent-protein/|title=Leprino Foods enters direct-to-consumer whey protein market with Ascent Protein|access-date=2016-06-01|date=23 May 2016}}

There is evidence that whey protein is more bio-available than casein or soy protein.Morifuji, Masashi (2010). "Comparison of Different Sources and Degrees of Hydrolysis of Dietary Protein: Effect on Plasma Amino Acids, Dipeptides, and Insulin Responses in Human Subjects". J. Agric. Food Chem. 58 (15): 8788–8797.Calbet, JA (2002). "Plasma glucagon and insulin responses depend on the rate of appearance of amino acids after ingestion of different protein solutions in humans".. J Nutr. 132 (8): 2174–82.

Whey protein is commonly marketed as a dietary supplement, typically sold in powdered form for mixing into beverages. Whey protein is also often used as a thickener to improve texture and decrease syneresis in yogurt. Greek Yogurt, which increased in popularity in the early twenty‐first century, is typically high in protein.{{Cite book|title=Whey Protein Production, Chemistry, Functionality, and Applications|last=Guo|first=Mingruo|publisher=John Wiley & Sons Ltd.|year=2019|isbn=9781119256052|location=Temple University|pages=119–122}}

The primary usage of whey protein supplements is for muscle growth and development. Eating whey protein supplements before exercise will not assist athletic performance, but it will enhance the body's protein recovery and synthesis after exercise because it increases the free amino acids in the body's free amino acid pool.

In 2010, a panel of the European Food Safety Authority (EFSA) panel examined the effects of whey protein on weight loss (via both fat loss and increased satiety) and strength and muscle building. The panel concluded that there's no evidence supporting any weight loss claims and that whey protein is roughly as effective for building strength, muscle and lean body mass as other protein sources.{{cite journal|last1=EFSA Panel on Dietetic Products, Nutrition and Allergies|title=Scientific Opinion on the substantiation of health claims related to whey protein |journal=EFSA Journal |date=2010 |volume=8 |issue=10 |pages=1818 |doi=10.2903/j.efsa.2010.1818 |doi-access=free }}

Although whey proteins are responsible for some milk allergies, the major allergens in milk are the caseins.{{cite journal |author=Wal JM |title=Bovine milk allergenicity |journal=Ann. Allergy Asthma Immunol. |volume=93 |issue=5 Suppl 3 |pages=S2–11 |date=2004 |pmid=15562868 |doi= 10.1016/S1081-1206(10)61726-7}}{{cite journal |vauthors=Burks W, Helm R, Stanley S, Bannon GA |title=Food allergens |journal=Curr Opin Allergy Clin Immunol |volume=1 |issue=3 |pages=243–248 |date=2001 |pmid=11964696 |doi=10.1097/01.all.0000011021.73682.01|s2cid=26812470 }}

{{main|Whey cheese}}

Whey cheese, such as ricotta, is produced from whey and is rich in whey protein (except for brunost). The whey protein accounts for about 40–45% of the solids content of ricotta.

{{reflist}}

• [http://myhealth.ucsd.edu/library/healthguide/en-us/Cam/topic.asp?hwid=hn-2933008 Whey Protein Healthnotes] {{Webarchive|url=https://web.archive.org/web/20091201180839/http://myhealth.ucsd.edu/library/healthguide/en-us/Cam/topic.asp?hwid=hn-2933008 |date=1 December 2009 }}, University of California, San Diego

Category:Bodybuilding supplements

Category:Dietary supplements

Category:Mammalian proteins

Category:Cheese