ZebraBox

{{Orphan|date=June 2024}}

The ZebraBox{{cite web | title = ZebraBox | url = http://www.viewpoint.fr/en/p/equipment/zebrabox | work = ViewPoint Life Sciences }} is an automated analysis chamber for the non-intrusive video observation of different types of freshwater marine indicator species, such as Dania rerio and Pimephales promelas. It is a type of Larval Photomoter Response (LPR) assay, which is used to monitor the swimming behaviour of larvae.{{cite book | vauthors = Dasgupta S, Simonich MT, Tanguay RL | title = High-Throughput Screening Assays in Toxicology | chapter = Zebrafish Behavioral Assays in Toxicology | volume = 2474 | pages = 109–122 | date = 2022 | pmid = 35294760 | doi = 10.1007/978-1-0716-2213-1_11 | isbn = 978-1-0716-2213-1 | series = Methods in Molecular Biology | veditors = Zhu H, Xia M | publisher = Springer US | place = New York, NY }}

The ZebraBox contains a controlled enclosed system of 96-well plates containing a high-resolution camera fitted with an infrared light and a fixed-angle lens.{{cite journal | vauthors = Lee DA, Oikonomou G, Prober DA | title = Large-scale Analysis of Sleep in Zebrafish | journal = Bio-Protocol | volume = 12 | issue = 3 | pages = e4313 | date = February 2022 | pmid = 35284597 | pmc = 8855086 | doi = 10.21769/BioProtoc.4313 }} The lighting conditions and illumination patterns can be manually controlled for fish acclimation and the simulation of circadian rhythms.{{cite journal | vauthors = Wang W, Ma Q, Ding X, Xu Y, He M, Xu J, Liu J, Ji C, Zhang J | display-authors = 6 | title = Developmental toxicity of bromoacetamide via the thyroid hormone receptors-mediated disruption of thyroid hormone homeostasis in zebrafish embryos | journal = Ecotoxicology and Environmental Safety | volume = 233 | pages = 113334 | date = March 2022 | pmid = 35203007 | doi = 10.1016/j.ecoenv.2022.113334 | s2cid = 247090575 | doi-access = free }} The apparatus allows for the analysis of zebrafish locomotion and activity, thus it is used in the fields of drug discovery and toxicological studies.{{cite journal | vauthors = Nasri A, Lafon PA, Mezni A, Clair P, Cubedo N, Mahmoudi E, Beyrem H, Rossel M, Perrier V | display-authors = 6 | title = Developmental exposure to the A6-pesticide causes changes in tyrosine hydroxylase gene expression, neurochemistry, and locomotors behavior in larval zebrafish | journal = Toxicology Mechanisms and Methods | pages = 569–579 | date = April 2022 | volume = 32 | issue = 8 | pmid = 35313786 | doi = 10.1080/15376516.2022.2056100 | s2cid = 247599353 }}{{cite journal | vauthors = Liu J, Xu Y, Liao G, Tu H, Huang Y, Peng T, Chen X, Huang Z, Zhang Y, Meng X, Zou F | display-authors = 6 | title = The role of ambra1 in Pb-induced developmental neurotoxicity in zebrafish | journal = Biochemical and Biophysical Research Communications | volume = 594 | pages = 139–145 | date = February 2022 | pmid = 35085890 | doi = 10.1016/j.bbrc.2021.12.084 | s2cid = 245496902 }}