random chimeragenesis on transient templates

Random chimeragenesis on transient templates (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate.{{cite journal|last=Coco|first=Wayne M.|author2=Levinson, William E. |author3=Crist, Michael J. |author4=Hektor, Harm J. |author5=Darzins, Aldis |author6=Pienkos, Philip T. |author7=Squires, Charles H. |author8= Monticello, Daniel J. |title=DNA shuffling method for generating highly recombined genes and evolved enzymes|journal=Nature Biotechnology|date=31 March 2001|volume=19|issue=4|pages=354–359|doi=10.1038/86744|pmid=11283594|s2cid=35360374 }} For example, RACHITT can be used to generate increased rate and extent of biodesulfurization of diesel by modification of dibenzothiophene mono-oxygenase. DNA shuffling is a similar but less powerful method used in directed evolution experiments.