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snRNP70

{{Short description|Protein-coding gene in the species Homo sapiens}}

{{lowercase title}}

{{Infobox_gene}}

snRNP70 also known as U1 small nuclear ribonucleoprotein 70 kDa is a protein{{cite journal | vauthors = Spritz RA, Strunk K, Surowy CS, Hoch SO, Barton DE, Francke U | title = The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding | journal = Nucleic Acids Research | volume = 15 | issue = 24 | pages = 10373–91 | date = December 1987 | pmid = 2447561 | pmc = 339950 | doi = 10.1093/nar/15.24.10373 }} that in humans is encoded by the SNRNP70 gene.{{cite web | title = Entrez Gene: SNRP70 small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=retrieve&dopt=default&list_uids=6625&rn=1}}{{cite journal | vauthors = Spritz RA, Strunk K, Surowy CS, Mohrenweiser HW | title = Human U1-70K ribonucleoprotein antigen gene: organization, nucleotide sequence, and mapping to locus 19q13.3 | journal = Genomics | volume = 8 | issue = 2 | pages = 371–9 | date = October 1990 | pmid = 2147422 | doi = 10.1016/0888-7543(90)90295-6 }} snRNP70 is a small nuclear ribonucleoprotein that associates with U1 spliceosomal RNA, forming the U1snRNP a core component of the spliceosome. The U1-70K protein and other components of the spliceosome complex form detergent-insoluble aggregates in both sporadic and familial human cases of Alzheimer's disease.{{cite journal | vauthors = Diner I, Hales CM, Bishof I, Rabenold L, Duong DM, Yi H, Laur O, Gearing M, Troncoso J, Thambisetty M, Lah JJ, Levey AI, Seyfried NT | title = Aggregation properties of the small nuclear ribonucleoprotein U1-70K in Alzheimer disease | journal = The Journal of Biological Chemistry | volume = 289 | issue = 51 | pages = 35296–313 | date = December 2014 | pmid = 25355317 | pmc = 4271217 | doi = 10.1074/jbc.M114.562959 | doi-access = free }}{{cite journal | vauthors = Hales CM, Seyfried NT, Dammer EB, Duong D, Yi H, Gearing M, Troncoso JC, Mufson EJ, Thambisetty M, Levey AI, Lah JJ | title = U1 small nuclear ribonucleoproteins (snRNPs) aggregate in Alzheimer's disease due to autosomal dominant genetic mutations and trisomy 21 | journal = Molecular Neurodegeneration | volume = 9 | pages = 15 | date = April 2014 | pmid = 24773620 | pmc = 4022210 | doi = 10.1186/1750-1326-9-15 | doi-access = free }} U1-70K co-localizes with Tau in neurofibrillary tangles in Alzheimer's disease.

Interactions

snRNP70 has been shown to interact with ASF/SF2,{{cite journal | vauthors = Xiao SH, Manley JL | title = Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2 | journal = The EMBO Journal | volume = 17 | issue = 21 | pages = 6359–67 | date = November 1998 | pmid = 9799243 | pmc = 1170960 | doi = 10.1093/emboj/17.21.6359 }}{{cite journal | vauthors = Cao W, Garcia-Blanco MA | title = A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding | journal = The Journal of Biological Chemistry | volume = 273 | issue = 32 | pages = 20629–35 | date = August 1998 | pmid = 9685421 | doi = 10.1074/jbc.273.32.20629 | doi-access = free }} SRPK1,{{cite journal | vauthors = Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC, Fu XD | title = SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells | journal = The Journal of Cell Biology | volume = 140 | issue = 4 | pages = 737–50 | date = February 1998 | pmid = 9472028 | pmc = 2141757 | doi = 10.1083/jcb.140.4.737 }}{{cite journal | vauthors = Kamachi M, Le TM, Kim SJ, Geiger ME, Anderson P, Utz PJ | title = Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway | journal = The Journal of Experimental Medicine | volume = 196 | issue = 9 | pages = 1213–25 | date = November 2002 | pmid = 12417631 | pmc = 2194102 | doi = 10.1084/jem.20021167 }} and ZRANB2.{{cite journal | vauthors = Adams DJ, van der Weyden L, Mayeda A, Stamm S, Morris BJ, Rasko JE | title = ZNF265--a novel spliceosomal protein able to induce alternative splicing | journal = The Journal of Cell Biology | volume = 154 | issue = 1 | pages = 25–32 | date = July 2001 | pmid = 11448987 | pmc = 2196870 | doi = 10.1083/jcb.200010059 }}

Role in autoimmunity

Antibodies towards snRNP70 are associated with mixed connective tissue disease.{{cite journal | vauthors = Sato T, Fujii T, Yokoyama T, Fujita Y, Imura Y, Yukawa N, Kawabata D, Nojima T, Ohmura K, Usui T, Mimori T | title = Anti-U1 RNP antibodies in cerebrospinal fluid are associated with central neuropsychiatric manifestations in systemic lupus erythematosus and mixed connective tissue disease | journal = Arthritis and Rheumatism | volume = 62 | issue = 12 | pages = 3730–40 | date = December 2010 | pmid = 20722023 | doi = 10.1002/art.27700 | hdl = 2433/142082 | hdl-access = free }}

References

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Further reading

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  • {{cite journal | vauthors = Wilk HE, Schaefer KP, Agris PF, Boak AM, Kovacs SA | title = U1 SnRNP association with HnRNP involves an initial non-specific splice-site independent interaction of U1 SnRNP protein with HnRNA | journal = Molecular and Cellular Biochemistry | volume = 106 | issue = 1 | pages = 55–66 | date = July 1991 | pmid = 1833625 | doi = 10.1007/BF00231189 | s2cid = 22696966 }}
  • {{cite journal | vauthors = Query CC, Bentley RC, Keene JD | title = A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein | journal = Cell | volume = 57 | issue = 1 | pages = 89–101 | date = April 1989 | pmid = 2467746 | doi = 10.1016/0092-8674(89)90175-X | s2cid = 22127152 }}
  • {{cite journal | vauthors = Du Clos TW | title = C-reactive protein reacts with the U1 small nuclear ribonucleoprotein | journal = Journal of Immunology | volume = 143 | issue = 8 | pages = 2553–9 | date = October 1989 | doi = 10.4049/jimmunol.143.8.2553 | pmid = 2477447 | s2cid = 84070596 | doi-access = free }}
  • {{cite journal | vauthors = Theissen H, Etzerodt M, Reuter R, Schneider C, Lottspeich F, Argos P, Lührmann R, Philipson L | title = Cloning of the human cDNA for the U1 RNA-associated 70K protein | journal = The EMBO Journal | volume = 5 | issue = 12 | pages = 3209–17 | date = December 1986 | pmid = 3028775 | pmc = 1167314 | doi = 10.1002/j.1460-2075.1986.tb04631.x}}
  • {{cite journal | vauthors = Romac JM, Graff DH, Keene JD | title = The U1 small nuclear ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain | journal = Molecular and Cellular Biology | volume = 14 | issue = 7 | pages = 4662–70 | date = July 1994 | pmid = 7516470 | pmc = 358839 | doi = 10.1128/MCB.14.7.4662}}
  • {{cite journal | vauthors = Wu JY, Maniatis T | title = Specific interactions between proteins implicated in splice site selection and regulated alternative splicing | journal = Cell | volume = 75 | issue = 6 | pages = 1061–70 | date = December 1993 | pmid = 8261509 | doi = 10.1016/0092-8674(93)90316-I | s2cid = 22951690 }}
  • {{cite journal | vauthors = Woppmann A, Will CL, Kornstädt U, Zuo P, Manley JL, Lührmann R | title = Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors | journal = Nucleic Acids Research | volume = 21 | issue = 12 | pages = 2815–22 | date = June 1993 | pmid = 8332490 | pmc = 309659 | doi = 10.1093/nar/21.12.2815 }}
  • {{cite journal | vauthors = Northemann W, Berg H, Stahnke G, Walter M, Hunt N, Fenning S | title = Identification of an inhibitory element within the human 68-kDa (U1) ribonucleoprotein antigen | journal = Protein Expression and Purification | volume = 6 | issue = 6 | pages = 748–56 | date = December 1995 | pmid = 8746626 | doi = 10.1006/prep.1995.0005 }}
  • {{cite journal | vauthors = Zhang WJ, Wu JY | title = Functional properties of p54, a novel SR protein active in constitutive and alternative splicing | journal = Molecular and Cellular Biology | volume = 16 | issue = 10 | pages = 5400–8 | date = October 1996 | pmid = 8816452 | pmc = 231539 | doi = 10.1128/MCB.16.10.5400}}
  • {{cite journal | vauthors = Bonaldo MF, Lennon G, Soares MB | title = Normalization and subtraction: two approaches to facilitate gene discovery | journal = Genome Research | volume = 6 | issue = 9 | pages = 791–806 | date = September 1996 | pmid = 8889548 | doi = 10.1101/gr.6.9.791 | doi-access = free }}
  • {{cite journal | vauthors = Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R | title = Association of U2 snRNP with the spliceosomal complex E | journal = Nucleic Acids Research | volume = 25 | issue = 2 | pages = 354–61 | date = January 1997 | pmid = 9016565 | pmc = 146436 | doi = 10.1093/nar/25.2.354 }}
  • {{cite journal | vauthors = Klein Gunnewiek JM, van Aarssen Y, van der Kemp A, Nelissen R, Pruijn GJ, van Venrooij WJ | title = Nuclear accumulation of the U1 snRNP-specific protein C is due to diffusion and retention in the nucleus | journal = Experimental Cell Research | volume = 235 | issue = 1 | pages = 265–73 | date = August 1997 | pmid = 9281376 | doi = 10.1006/excr.1997.3663 }}
  • {{cite journal | vauthors = Zhang WJ, Wu JY | title = Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing | journal = Molecular and Cellular Biology | volume = 18 | issue = 2 | pages = 676–84 | date = February 1998 | pmid = 9447963 | pmc = 108778 | doi = 10.1128/MCB.18.2.676}}
  • {{cite journal | vauthors = Blencowe BJ, Issner R, Nickerson JA, Sharp PA | title = A coactivator of pre-mRNA splicing | journal = Genes & Development | volume = 12 | issue = 7 | pages = 996–1009 | date = April 1998 | pmid = 9531537 | pmc = 316672 | doi = 10.1101/gad.12.7.996 }}
  • {{cite journal | vauthors = Cao W, Garcia-Blanco MA | title = A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding | journal = The Journal of Biological Chemistry | volume = 273 | issue = 32 | pages = 20629–35 | date = August 1998 | pmid = 9685421 | doi = 10.1074/jbc.273.32.20629 | doi-access = free }}
  • {{cite journal | vauthors = Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M | title = Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex | journal = Nature Genetics | volume = 20 | issue = 1 | pages = 46–50 | date = September 1998 | pmid = 9731529 | doi = 10.1038/1700 | s2cid = 585778 }}
  • {{cite journal | vauthors = Xiao SH, Manley JL | title = Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2 | journal = The EMBO Journal | volume = 17 | issue = 21 | pages = 6359–67 | date = November 1998 | pmid = 9799243 | pmc = 1170960 | doi = 10.1093/emboj/17.21.6359 }}
  • {{cite journal | vauthors = Dumortier H, Klein Gunnewiek J, Roussel JP, van Aarssen Y, Briand JP, van Venrooij WJ, Muller S | title = At least three linear regions but not the zinc-finger domain of U1C protein are exposed at the surface of the protein in solution and on the human spliceosomal U1 snRNP particle | journal = Nucleic Acids Research | volume = 26 | issue = 23 | pages = 5486–91 | date = December 1998 | pmid = 9826776 | pmc = 147990 | doi = 10.1093/nar/26.23.5486 }}

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{{Ribonucleoproteins}}

Category:Protein complexes

Category:Spliceosome

Category:RNA splicing