synthetic rescue

Overexpression of a gene compensates for a loss-of-function mutation, for example, extra HIS4 copies rescuing his4 auxotrophy in yeast.

A mutation in one gene compensates for another, for example; SRS2 deletion rescuing rad54Δ lethality in yeast.

A suppressor mutation activates an alternative pathway to bypass a defect, for example; EXO1 deletion rescuing cdc13-1 by halting telomere degradation in mutant yeast.{{Cite journal |last1=Wan |first1=Yue |last2=Qu |first2=Kun |last3=Ouyang |first3=Zhengqing |last4=Kertesz |first4=Michael |last5=Li |first5=Jun |last6=Tibshirani |first6=Robert |last7=Makino |first7=Debora L. |last8=Nutter |first8=Robert C. |last9=Segal |first9=Eran |last10=Chang |first10=Howard Y. |date=2012-10-26 |title=Genome-wide measurement of RNA folding energies |journal=Molecular Cell |volume=48 |issue=2 |pages=169–181 |doi=10.1016/j.molcel.2012.08.008 |issn=1097-4164 |pmc=3483374 |pmid=22981864}}

Synthetic rescue principles underpin PARP inhibitor treatments in BRCA-deficient cancers. While PARP inhibition is synthetic lethal with BRCA loss, synthetic rescue interactions such as 53BP1 deletion restoring viability reveal resistance mechanisms and alternative targets.{{Cite journal |last=Zlotorynski |first=Eytan |date=August 2016 |title=The dark side of p21 |url=https://www.nature.com/articles/nrc.2016.78 |journal=Nature Reviews Cancer |language=en |volume=16 |issue=8 |pages=481 |doi=10.1038/nrc.2016.78 |pmid=27417653 |issn=1474-1768|url-access=subscription }}

• Engineered Redundancy: One study introduced an engineered mutation in an industrial yeast strain with redundant pathways ADH2 rescue of thermal sensitive yeast with defect in sterol metabolism to improve metabolic engineering robustness.{{Cite journal |last1=Nelson |first1=Shane R. |last2=Dunn |first2=Andrew R. |last3=Kathe |first3=Scott D. |last4=Warshaw |first4=David M. |last5=Wallace |first5=Susan S. |date=2014-05-20 |title=Two glycosylase families diffusively scan DNA using a wedge residue to probe for and identify oxidatively damaged bases |journal=Proceedings of the National Academy of Sciences |volume=111 |issue=20 |pages=E2091–E2099 |doi=10.1073/pnas.1400386111 |doi-access=free |pmc=4034194 |pmid=24799677|bibcode=2014PNAS..111E2091N }}
• Targeting Antibiotic Resistance: Another study found E. coli with recA deletions are rescued by lexA mutations, informing strategies to combat resistance by targeting compensatory networks like lexA mutations.{{Cite journal |last1=Cirz |first1=Ryan T. |last2=Chin |first2=Jodie K. |last3=Andes |first3=David R. |last4=Crécy-Lagard |first4=Valérie de |last5=Craig |first5=William A. |last6=Romesberg |first6=Floyd E. |date=2005-05-10 |title=Inhibition of Mutation and Combating the Evolution of Antibiotic Resistance |journal=PLOS Biology |language=en |volume=3 |issue=6 |pages=e176 |doi=10.1371/journal.pbio.0030176 |doi-access=free |issn=1545-7885 |pmc=1088971 |pmid=15869329}}

The Systems Metabolic Engineering Group of KAIST engineered synthetic rescue in E.coli by deleting sdhA and compensating with mutations in icd for the purpose of rescuing lethal metabolic pathways with the goal of expanding the scope of genome-scale engineering and developing platform technologies for sustainable biochemical production.{{Cite journal |last1=Ni |first1=Bin |last2=Colin |first2=Remy |last3=Sourjik |first3=Victor |date=2021-06-18 |title=Production and Characterization of Motile and Chemotactic Bacterial Minicells |journal=ACS Synthetic Biology |language=en |volume=10 |issue=6 |pages=1284–1291 |doi=10.1021/acssynbio.1c00012 |issn=2161-5063 |pmc=8218304 |pmid=34081866}}

• Complex networks
• Gene therapy
• Suppressor mutation
• Synthetic lethality

{{reflist}}

Category:Gene therapy