transposon tagging
In genetic engineering, transposon tagging is a process where transposons (transposable elements) are amplified inside a biological cell by a tagging technique. Transposon tagging has been used with several species to isolate genes.{{cite web |first=Paaras V. |last=Shah |year=2007 |title=Transposable Elements |url=http://arabidopsis.info/students/paaras/transposable_elements.htm}}{{Self-published inline|date=March 2011}} Even without knowing the nature of the specific genes, the process can still be used.
In plants
By molecular separation of transposons, from a cell nucleus, the cloning is enabled for genes which contain the transposons.{{cite web |title=Transposon Tagging |first=Phillip |last=McClean |year=1998 |url=http://www.ndsu.edu/pubweb/~mcclean/plsc731/transposon/tag4.htm}}{{Self-published inline|date=March 2011}}
By using transposon tagging, researchers have been able to add genetic elements from maize (corn){{cite journal |last1=Brutnell |first1=Thomas |title=Transposon tagging in maize |journal=Functional & Integrative Genomics |volume=2 |issue=1–2 |pages=4–12 |year=2002 |pmid=12021846 |doi=10.1007/s10142-001-0044-0|s2cid=23266030 }} and Antirrhinum into some other species (such as tobacco,{{cite journal |doi=10.1073/pnas.92.10.4175 |last1=Dinesh-Kumar |first1=S. P. |last2=Whitham |first2=S. |last3=Choi |first3=D. |last4=Hehl |first4=R. |last5=Corr |first5=C. |last6=Baker |first6=B. |title=Transposon Tagging of Tobacco Mosaic Virus Resistance Gene N: Its Possible Role in the TMV-N-Mediated Signal Transduction Pathway |journal=Proceedings of the National Academy of Sciences of the United States of America |volume=92 |issue=10 |pages=4175–4180 |year=1995 |pmid=7753780 |pmc=41906 |jstor=2367289|bibcode=1995PNAS...92.4175D |doi-access=free }} aspen{{cite journal |last1=Fladung |first1=M |last2=Deutsch |first2=F |last3=Hönicka |first3=H |last4=Kumar |first4=S |title=T-DNA and Transposon Tagging in Aspen |journal=Plant Biology |volume=6 |issue=1 |pages=5–11 |year=2004 |pmid=15095129 |doi=10.1055/s-2003-44745 |s2cid=260252516 }} and others). A gene responsible for a particular phenotype can be cloned within a given species, when movement is accompanied by the presence of a mutant phenotype.
References
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Further reading
- [http://www.ndsu.edu/pubweb/~mcclean/plsc731/transposon/tag4.htm Transposon Tagging], North Dakota State University
- A.M. Settles, [http://hos.ufl.edu/sites/default/files/faculty/amsettles/publications/2009.Kriz_CH-11_Final.pdf Transposon Tagging and Reverse Genetics], University of Florida
Category:Biochemistry detection methods
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