water blue
{{For|the butterfly with this common name|Cacyreus tespis{{!}}Cacyreus tespis}}
{{refimprove|date=June 2013}}
{{chembox
| Watchedfields = changed
| verifiedrevid = 417216787
| ImageFile = Water blue2.png
| ImageSize =
| IUPACName =
| OtherNames =
|Section1={{Chembox Identifiers
| CASNo_Ref = {{cascite|correct|??}}
| CASNo = 28631-66-5
| ChemSpiderID = 21173243
| EINECS = 249-113-9
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = 44D829LK4D
| PubChem = 139030972
| SMILES = [H+].c1ccc(c(c1)Nc2ccc(cc2)C(=C3C=CC(=Nc4ccccc4S(=O)(=O)[O-])C=C3)c5cccc(c5CN)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
| StdInChI=1S/C32H27N3O9S3.2Na/c33-20-26-25(6-5-11-29(26)45(36,37)38)32(21-12-16-23(17-13-21)34-27-7-1-3-9-30(27)46(39,40)41)22-14-18-24(19-15-22)35-28-8-2-4-10-31(28)47(42,43)44;;/h1-19,34H,20,33H2,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2
| StdInChIKey = WJHQBPPQVMBLSW-UHFFFAOYSA-L
}}
|Section2={{Chembox Properties
| Formula = C32H25N3O9S3Na2
| MolarMass = 737.72374 g/mol
| Appearance =
| Density =
| MeltingPt =
| BoilingPt =
| Solubility =
}}
|Section3={{Chembox Hazards
| GHSPictograms = {{GHS07}}
| GHSSignalWord = Warning
| HPhrases = {{H-phrases|315|317|319|335}}
| PPhrases = {{P-phrases|}}
| MainHazards =
| FlashPt =
| AutoignitionPt =
}}
}}
Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol.
Water blue is also available in mixture with methyl blue, under the names Aniline Blue WS, Aniline blue, China blue, or Soluble blue.{{cite web |year= 1969 |title= H. J. Conn's Biological stains : a handbook on the nature and uses of the dyes employed in the biological laboratory |author= Lillie, R. D. |url= https://trove.nla.gov.au/work/21134552?selectedversion=NBD35338 |access-date= February 8, 2019 |website= Trove (National Library of Australia)}}{{cite web |title= Aniline Blue WS |website= StainsFile |url= http://stainsfile.info/StainsFile/dyes/707.htm }}
It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining.{{Citation|title= A simple dual stain for detailed investigations of plant-fungal pathogen interactions |last=Nowicki|first=Marcin|journal= Vegetable Crops Research Bulletin |date=15 May 2013|volume= 77 |pages= 61–74 |publisher=Vegetable Crops Research bulleting, InHort & Versita|doi= 10.2478/v10032-012-0016-z|display-authors=etal|doi-access=free}}