:CHES (buffer)
{{chembox
| verifiedrevid = 444020887
| Name=CHES
| ImageFile=N-Cyclohexyl-2-aminoethanesulfonic acid.svg
| ImageSize=
| PIN=2-(Cyclohexylamino)ethane-1-sulfonic acid
| OtherNames= N-Cyclohexyl-2-aminoethanesulfonic acid; N-Cyclohexyltaurine
|Section1={{Chembox Identifiers
| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID = 60260
| ChEBI = 44302
| InChI = 1/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)
| InChIKey = MKWKNSIESPFAQN-UHFFFAOYAH
| StdInChI_Ref = {{stdinchicite|correct|chemspider}}
| StdInChI = 1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)
| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}}
| StdInChIKey = MKWKNSIESPFAQN-UHFFFAOYSA-N
| CASNo_Ref = {{cascite|correct|??}}
| CASNo=103-47-9
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = 71X53V3RZ1
| PubChem=66898
| DrugBank_Ref = {{drugbankcite|correct|drugbank}}
| DrugBank = DB03309
| SMILES = O=S(=O)(O)CCNC1CCCCC1
| MeSHName=2-(N-cyclohexylamino)ethanesulfonic+acid
}}
|Section2={{Chembox Properties
| C = 8 | H = 17 | N = 1 | O = 3 | S = 1
| Appearance=
| Density=
| MeltingPt=
| BoilingPt=
| Solubility=
| pKa = 9.3
}}
|Section3={{Chembox Hazards
| MainHazards=
| FlashPt=
| AutoignitionPt =
}}
}}
CHES (N-cyclohexyl-2-aminoethanesulfonic acid) is a buffering agent. CHES buffers have a useful range of pH 8.6–10.{{cite web |title=Good's buffers (biological buffers) |url=https://www.interchim.fr/ft/0/062000.pdf}}
It typically appears as a white crystalline powder.
Effect of impurities
Commercial prep of CHES (and other sulfonylethyl buffers like MES, BES, and PIPES) can contain a contaminant oligo(vinylsulfonic acid) (OVS), which is a polyanionic mimic of RNA, and can be a potent (pM) inhibitor of RNA binding proteins and enzymes.{{cite journal |last=Smith |first=Bryan D. |last2=Soellner |first2=Matthew B. |last3=Raines |first3=Ronald T. |title=Potent Inhibition of Ribonuclease A by Oligo(vinylsulfonic Acid) |journal=Journal of Biological Chemistry |publisher=Elsevier BV |volume=278 |issue=23 |year=2003 |issn=0021-9258 |pages=20934–20938 |doi=10.1074/jbc.m301852200 |doi-access=free}}
References
{{Reflist}}
External links
- {{DrugBank|EXPT02344}}
{{DEFAULTSORT:Cyclohexyl-2-aminoethanesulfonic acid, N-}}
{{Organic-compound-stub}}