:CHES (buffer)

{{chembox

| verifiedrevid = 444020887

| Name=CHES

| ImageFile=N-Cyclohexyl-2-aminoethanesulfonic acid.svg

| ImageSize=

| PIN=2-(Cyclohexylamino)ethane-1-sulfonic acid

| OtherNames= N-Cyclohexyl-2-aminoethanesulfonic acid; N-Cyclohexyltaurine

|Section1={{Chembox Identifiers

| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}

| ChemSpiderID = 60260

| ChEBI = 44302

| InChI = 1/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

| InChIKey = MKWKNSIESPFAQN-UHFFFAOYAH

| StdInChI_Ref = {{stdinchicite|correct|chemspider}}

| StdInChI = 1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}}

| StdInChIKey = MKWKNSIESPFAQN-UHFFFAOYSA-N

| CASNo_Ref = {{cascite|correct|??}}

| CASNo=103-47-9

| UNII_Ref = {{fdacite|correct|FDA}}

| UNII = 71X53V3RZ1

| PubChem=66898

| DrugBank_Ref = {{drugbankcite|correct|drugbank}}

| DrugBank = DB03309

| SMILES = O=S(=O)(O)CCNC1CCCCC1

| MeSHName=2-(N-cyclohexylamino)ethanesulfonic+acid

}}

|Section2={{Chembox Properties

| C = 8 | H = 17 | N = 1 | O = 3 | S = 1

| Appearance=

| Density=

| MeltingPt=

| BoilingPt=

| Solubility=

| pKa = 9.3

}}

|Section3={{Chembox Hazards

| MainHazards=

| FlashPt=

| AutoignitionPt =

}}

}}

CHES (N-cyclohexyl-2-aminoethanesulfonic acid) is a buffering agent. CHES buffers have a useful range of pH 8.6–10.{{cite web |title=Good's buffers (biological buffers) |url=https://www.interchim.fr/ft/0/062000.pdf}}

It typically appears as a white crystalline powder.

Effect of impurities

Commercial prep of CHES (and other sulfonylethyl buffers like MES, BES, and PIPES) can contain a contaminant oligo(vinylsulfonic acid) (OVS), which is a polyanionic mimic of RNA, and can be a potent (pM) inhibitor of RNA binding proteins and enzymes.{{cite journal |last=Smith |first=Bryan D. |last2=Soellner |first2=Matthew B. |last3=Raines |first3=Ronald T. |title=Potent Inhibition of Ribonuclease A by Oligo(vinylsulfonic Acid) |journal=Journal of Biological Chemistry |publisher=Elsevier BV |volume=278 |issue=23 |year=2003 |issn=0021-9258 |pages=20934–20938 |doi=10.1074/jbc.m301852200 |doi-access=free}}

References

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