PIPES

{{about|the biochemical buffer PIPES|other uses|Pipe (disambiguation)}}

{{chembox

| Verifiedfields = changed

| Watchedfields = changed

| verifiedrevid = 450879654

| Name = PIPES

| ImageFile = PIPES.svg

| ImageName = Chemical structure of PIPES

| PIN = 2,2{{prime}}-(Piperazine-1,4-diyl)di(ethane-1-sulfonic acid)

| OtherNames = PIPES

|Section1={{Chembox Identifiers

| CASNo_Ref = {{cascite|correct|??}}

| CASNo = 5625-37-6

| UNII_Ref = {{fdacite|correct|FDA}}

| UNII = G502H79V6L

| PubChem = 6992709

| ChemSpiderID_Ref = {{chemspidercite|changed|chemspider}}

| ChemSpiderID = 72022

| SMILES = C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

| InChI = 1/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

| InChIKey = IHPYMWDTONKSCO-UHFFFAOYAG

| StdInChI_Ref = {{stdinchicite|changed|chemspider}}

| StdInChI = 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

| StdInChIKey_Ref = {{stdinchicite|changed|chemspider}}

| StdInChIKey = IHPYMWDTONKSCO-UHFFFAOYSA-N

}}

|Section2={{Chembox Properties

| Formula = C₈H₁₈N₂O₆S₂

| MolarMass = 302.37

| Appearance = White powder

| Solubility = 1 g/L (100 °C)

| MeltingPt = Decomposes above 300 °C

| BoilingPt = Decomposes

}}

|Section7={{Chembox Hazards

| ExternalSDS = [http://hazard.com/msds/mf/baker/baker/files/p4789.htm External MSDS]

| MainHazards = Irritant

| NFPA-H = 1

| NFPA-R = 0

| NFPA-F = 0

}}

}}

PIPES (piperazine-N,N{{prime}}-bis(2-ethanesulfonic acid)) is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.{{cite journal|doi=10.1021/bi00866a011|title=Hydrogen Ion Buffers for Biological Research|year=1966|last1=Good|first1=Norman E.|last2=Winget|first2=G. Douglas|last3=Winter|first3=Wilhelmina|last4=Connolly|first4=Thomas N.|last5=Izawa|first5=Seikichi|last6=Singh|first6=Raizada M. M.|journal=Biochemistry|volume=5|pages=467–77|pmid=5942950|issue=2}}