AFO

A Funk Odyssey
Academia Film Olomouc, a Czech film festival
Advanced Force Operations, a US military term
Aero Force One, a fan club for the rock band Aerosmith
Afghan Film Organization, a film production company and archive.
Afo Dodoo (born 1973), Ghanaian footballer
AFO Records, the first African-American owned record label in the US
Air Force One (disambiguation)
Alien Front Online, a video game
Animal Face-Off, a television show
Animal feeding operation, as defined by the Environmental Protection Agency
Anime Festival Orlando, a yearly anime convention in Orlando, Florida
Ankle-foot orthosis, a brace for the ankle and foot
Anti-Fascist Organisation, a provisional organization in Burma during World War II
Arellano-Felix Organization, a drug trafficking cartel in Mexico, also known as the Tijuana Cartel
Assemblée de la francophonie de l'Ontario, a Canadian cultural organization
Association of Field Ornithologists, an ornithological organization, publishers of Journal of Field Ornithology
Australian Field Ornithology, a journal of BirdLife Australia
Authorised Firearms Officer, a police officer in the UK who has received training and authorization to carry and use firearms
Eloyi language (ISO-639: afo)
Eloyi people, also called Afo
Afo, thought to be the world's oldest Clove Tree