Baby hamster kidney cell
Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology.
The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.
BHK and virus infection
BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain).{{cite journal | vauthors = Timm C, Gupta A, Yin J | title = Robust kinetics of an RNA virus: Transcription rates are set by genome levels | journal = Biotechnology and Bioengineering | volume = 112 | issue = 8 | pages = 1655–62 | date = August 2015 | pmid = 25726926 | pmc = 5653219 | doi = 10.1002/bit.25578 }}
BHK-21 cells are resistant to poliovirus 2 and Rabbit vesivirus (RaV). The cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes.{{cite web|title=BHK-21 Cells|url=https://altogen.com/product/bhk-21-transfection-reagent-kidney-cells-ccl-10/|access-date=24 April 2018}}
Utilization
The BHK-21 cells are useful for transformations and for stable and temporary transfections. BHK cells are also used to study viral infections.{{cite journal | vauthors = Lim KI, Lang T, Lam V, Yin J | title = Model-based design of growth-attenuated viruses | journal = PLOS Computational Biology | volume = 2 | issue = 9 | pages = e116 | date = September 2006 | pmid = 16948530 | pmc = 1557587 | doi = 10.1371/journal.pcbi.0020116 | bibcode = 2006PLSCB...2..116L | doi-access = free }}
Recommended growth medium
- High-glucose DMEM
- FBS fetal bovine serum: 5% (15% when freeze)
- GLU glutamine: 1%
- PSA regular antibiotics: 1%
- Splitting: 10 by trypsin{{cite web |title=Microscopy Resource Center {{!}} Olympus Life Science |url=http://www.olympusmicro.com/primer/techniques/fluorescence/gallery/cells/bhk/bhkcells.html |website=www.olympusmicro.com |access-date=2009-10-31 |archive-date=2009-12-27 |archive-url=https://web.archive.org/web/20091227190844/http://www.olympusmicro.com/primer/techniques/fluorescence/gallery/cells/bhk/bhkcells.html |url-status=dead }}
References
{{Reflist}}
External links
- [http://www.cellosaurus.org/CVCL_1914 Cellosaurus entry for BHK-21]
- [http://www.cellosaurus.org/CVCL_1915 Cellosaurus entry for BHK-21 clone 13 (most used derivative)]