Bioarchaeology

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Paleodemography studies demographic characteristics of past populations.{{Cite book|date=2002-01-03|editor-last=Hoppa|editor-first=Robert D.|editor2-last=Vaupel|editor2-first=James W.|title=Paleodemography|url=http://dx.doi.org/10.1017/cbo9780511542428|doi=10.1017/cbo9780511542428|isbn=9780521800631}} Bioarchaeologists use paleodemography to create life tables, a type of cohort analysis, to understand zdemographic characteristics (such as risk of death or sex ratio) of a given age cohort within a population. It is often necessary to estimate the age and sex of individuals based on specific morphological characteristics of the skeleton.

Age estimation attempts to determine the skeletal/biological age-at-death. The primary assumption is that an individual's skeletal age is closely associated with their chronological age. Age estimation can be based on patterns of growth and development or degenerative changes in the skeleton.{{Citation|last1=Robling|first1=Alexander G.|title=Histomorphometry of Human Cortical Bone: Applications to Age Estimation|url=http://dx.doi.org/10.1002/9780470245842.ch5|work=Biological Anthropology of the Human Skeleton|pages=149–182|place=Hoboken, NJ, USA|publisher=John Wiley & Sons, Inc.|isbn=978-0-470-24584-2|access-date=2020-12-05|last2=Stout|first2=Sam D.|year=2008|doi=10.1002/9780470245842.ch5|url-access=subscription}} A variety of skeletal series methods to assess these types of changes have been developed. For instance, in children age is typically estimated by assessing dental development, ossification and fusion of specific skeletal elements, or long bone length.{{cite book|title=Human skeletal remains: excavation, analysis, interpretation (3rd ed.)|last=Ubelaker|first=D.H.|publisher=Taraxacum|year=1999|location=Washington, DC}} For children, different teeth erupt from the gums serially are the most reliable for telling a child's age. However, fully developed teeth are less indicative.{{Cite web|url=https://www.nhm.ac.uk/discover/analysing-the-bones-what-can-a-skeleton-tell-you.html|title=Analysing the bones: what can a skeleton tell you?|last=Dunning|first=Haley|publisher=Natural History Museum, London|language=en|access-date=2020-12-05}} In adults, degenerative changes to the pubic symphysis, the auricular surface of the ilium, the sternal end of the 4th rib, and dental attrition are commonly used to estimate skeletal age.{{cite book|title=Standards for data collection from human skeletal remains|last=Buikstra|first=J.E.|author2=D.H. Ubelaker|publisher=Arkansas Archaeological Survey|year=1994|page=208}}{{cite journal|last=Lovejoy|first=C.O.|author2=Meindl, R.S.|author3=Pryzbeck, T.R.|author4=Mensforth R.P.|year=1985|title=Chronological metamorphosis of the auricular surface of the ilium: a new method for the determination of adult skeletal age at death|journal=American Journal of Physical Anthropology|volume=68|issue=1|pages=15–28|doi=10.1002/ajpa.1330680103|pmid=4061599}}{{cite journal|last=Lovejoy|first=C.O.|year=1985|title=Dental wear in the Libben population: its functional pattern and role in the determination of adult skeletal age at death|journal=American Journal of Physical Anthropology|volume=68|issue=1|pages=47–56|doi=10.1002/ajpa.1330680105|pmid=4061601}}

Until the age of about 30, human bones keep growing. Different bones fuse at different points of growth.{{Cite web|title=How Bones Develop|url=https://whenithurtstomove.org/about-orthopaedics/how-bones-develop/|access-date=2020-12-05|website=Canadian Orthopaedic Foundation|language=en-CA|archive-url=https://web.archive.org/web/20201202224343/https://whenithurtstomove.org/about-orthopaedics/how-bones-develop/|archive-date=2020-12-02}} This development can vary acros individuals. Wear and tear on bones further complicates age estimates. Often, estimates are limited to 'young' (20–35 years), 'middle' (35–50 years), or 'old' (50+ years).

Differences in male and female skeletal anatomy are used by bioarchaeologists to determine the biological sex of human skeletons. Humans are sexually dimorphic, although overlap in body shape and sexual characteristics is possible. Not all skeletons can be assigned a sex, and some may be wrongly identified. Biological males and biological females differ most in the skull and pelvis; bioarchaeologists focus on these body parts, although other body parts can be used. The female pelvis is generally broader than the male pelvis, and the angle between the two inferior pubic rami (the sub-pubic angle) is wider and more U-shaped, while the sub-pubic angle of the male is more V-shaped and less than 90 degrees.{{cite journal |last1=Phenice |first1=T.W. |year=1969 |title=A Newly Developed Visual Method of Sexing the Os Pubis |journal=American Journal of Physical Anthropology |volume=30 |issue=2 |pages=297–302 |doi=10.1002/ajpa.1330300214 |pmid=5772048}}

In general, the male skeleton is more robust than the female skeleton because of male's greater muscles mass. Male skeletons generally have more pronounced brow ridges, nuchal crests, and mastoid processes. Skeletal size and robustness are influenced by nutrition and activity levels. Pelvic and cranial features are considered to be more reliable indicators of biological sex. Sexing skeletons of young people who have not completed puberty is more difficult and problematic, because the body has not fully developed.

Bioarchaeological sexing of skeletons is not error-proof. Recording errors and re-arranging of human remains may play a part in such misidentification.

Direct testing of bioarchaeological methods for sexing skeletons by comparing gendered names on coffin plates from the crypt at Christ Church, Spitalfields, London to the associated remains achieved a 98 percent success rate.Molleson, Theya, and M. Cox. The Spitalfields Project, Volume 2: The Anthropology: Council For British Archaeology, York, 1993.

Gendered work patterns may leave marks on bones and be identifiable in the archaeological record. One study found extremely arthritic big toes, a collapse of the last dorsal vertebrae, and muscular arms and legs among female skeletons at Abu Hureyra, interpreting this as indicative of gendered work patterns.{{cite journal |last1=Molleson |first1=Theya |year=1994 |title=The Eloquent Bones of Abu Hureyra |journal=Scientific American |volume=271 |issue=2 |pages=70–75 |bibcode=1994SciAm.271b..70M |doi=10.1038/scientificamerican0894-70 |pmid=8066433}} Such skeletal changes could have resulted from women spending long periods kneeling while grinding grain with the toes curled forward. Investigation of gender from mortuary remains is of growing interest to archaeologists.{{Cite book |last1=Arnold |first1=Bettina |url={{google books|plainurl=y|id=6hVz6RzCkJcC}} |title=Gender and the Archaeology of Death |last2=Wicker |first2=Nancy L. |date=2001 |publisher=Rowman Altamira |isbn=978-0-7591-0137-1 |language=en}}

Recent developments in bioarchaeological methods have introduced more accurate and standardized techniques for sex estimation, especially when skeletal preservation is poor. Metric analyses of pelvic morphology using tools such as the Diagnose Sexuelle Probabiliste (DSP) method have achieved over 95% accuracy in adult individuals when analyzing the os coxae, using discriminant functions based on population-specific reference data.{{Cite journal |last1=Brůžek |first1=Jaroslav |last2=Santos |first2=Frédéric |last3=Dutailly |first3=Bruno |last4=Murail |first4=Pascal |last5=Cunha |first5=Eugenia |date=2017 |title=Validation and reliability of the sex estimation of the human os coxae using freely available DSP2 software for bioarchaeology and forensic anthropology |url=https://pubmed.ncbi.nlm.nih.gov/28714560 |journal=American Journal of Physical Anthropology |volume=164 |issue=2 |pages=440–449 |doi=10.1002/ajpa.23282 |issn=1096-8644 |pmid=28714560}} Geometric morphometric analyses of cranial and pelvic landmarks, particularly when paired with statistical classifiers or machine learning algorithms, have also shown high success rates in identifying sex across both forensic and archaeological samples.{{Cite journal |last1=Imaizumi |first1=Kazuhiko |last2=Bermejo |first2=Enrique |last3=Taniguchi |first3=Kei |last4=Ogawa |first4=Yoshinori |last5=Nagata |first5=Takeshi |last6=Kaga |first6=Kazunori |last7=Hayakawa |first7=Hideyuki |last8=Shiotani |first8=Seiji |date=2020-09-01 |title=Development of a sex estimation method for skulls using machine learning on three-dimensional shapes of skulls and skull parts |url=https://www.sciencedirect.com/science/article/pii/S2666225620300427 |journal=Forensic Imaging |volume=22 |article-number=200393 |doi=10.1016/j.fri.2020.200393 |issn=2666-2256|url-access=subscription }} Molecular techniques have also become integrated into bioarchaeological practice. Ancient DNA (aDNA) shotgun sequencing enables near-perfect sex determination by quantifying X- and Y-chromosome reads, proving especially valuable when osteological indicators are absent or ambiguous.{{Cite journal |last1=Skoglund |first1=Pontus |last2=Storå |first2=Jan |last3=Götherström |first3=Anders |last4=Jakobsson |first4=Mattias |date=2013-12-01 |title=Accurate sex identification of ancient human remains using DNA shotgun sequencing |url=https://www.sciencedirect.com/science/article/pii/S0305440313002495 |journal=Journal of Archaeological Science |volume=40 |issue=12 |pages=4477–4482 |doi=10.1016/j.jas.2013.07.004 |bibcode=2013JArSc..40.4477S |issn=0305-4403|url-access=subscription }} Where DNA preservation is insufficient, dental proteomics as detecting amelogenin peptides in tooth enamel provides a minimally destructive and highly reliable alternative for sex estimation.{{Cite journal |last1=Stewart |first1=Nicolas Andre |last2=Gerlach |first2=Raquel Fernanda |last3=Gowland |first3=Rebecca L. |last4=Gron |first4=Kurt J. |last5=Montgomery |first5=Janet |date=2017-12-26 |title=Sex determination of human remains from peptides in tooth enamel |journal=Proceedings of the National Academy of Sciences |volume=114 |issue=52 |pages=13649–13654 |doi=10.1073/pnas.1714926115 |doi-access=free |pmc=5748210 |pmid=29229823|bibcode=2017PNAS..11413649S }}

Dental non-specific stress indicators are features found on teeth that reflect episodes of physiological stress experienced during childhood, particularly during the period of enamel formation. They are described as "non-specific" because, while they signal that a stress event occurred, they do not identify the exact cause, such as whether it resulted from malnutrition, illness, or infection.

Enamel forms through a process called amelogenesis, carried out by specialized cells known as ameloblasts, which produce enamel in sequential layers. When these cells are affected by systemic stress, the enamel formation process can be interrupted or altered, resulting in visible developmental defects.{{Cite web |title=Complexity of human tooth enamel revealed at atomic level in NIH-funded study {{!}} National Institutes of Health (NIH) |url=https://www.nih.gov/news-events/news-releases/complexity-human-tooth-enamel-revealed-atomic-level-nih-funded-study |access-date=2025-06-18 |website=www.nih.gov |language=en}}{{Cite journal |last1=Davis |first1=Kathryn A. |last2=Mountain |first2=Rebecca V. |last3=Pickett |first3=Olivia R. |last4=Den Besten |first4=Pamela K. |last5=Bidlack |first5=Felicitas B. |last6=Dunn |first6=Erin C. |date=2020-03-15 |title=Teeth as Potential New Tools to Measure Early-Life Adversity and Subsequent Mental Health Risk: An Interdisciplinary Review and Conceptual Model |journal=Biological Psychiatry |volume=87 |issue=6 |pages=502–513 |doi=10.1016/j.biopsych.2019.09.030 |issn=1873-2402 |pmc=7822497 |pmid=31858984}}{{Cite journal |last1=Costiniti |first1=Veronica |last2=Bomfim |first2=Guilherme H. |last3=Mitaishvili |first3=Erna |last4=Son |first4=Ga-Yeon |last5=Li |first5=Yi |last6=Lacruz |first6=Rodrigo S. |date=2021 |title=Calcium Transport in Specialized Dental Epithelia and Its Modulation by Fluoride |journal=Frontiers in Endocrinology |volume=12 |pages=730913 |doi=10.3389/fendo.2021.730913 |doi-access=free |issn=1664-2392 |pmc=8385142 |pmid=34456880}}

Enamel hypoplasia refers to transverse furrows or pits that form in the enamel surface of teeth when the normal process of tooth growth stops, leaving a deficit. Enamel hypoplasias generally form due to disease and/or poor nutrition.Mays, Simon. The Archaeology of Human Bones. 1998. Second ed. New York: Routledge, 2010. 2010. Linear furrows are commonly referred to as linear enamel hypoplasias (LEHs); LEHs can range in size from microscopic to visible to the naked eye. By examining the spacing of perikymata grooves (horizontal growth lines), the duration of the stressor can be estimated,Humphrey, Louise T. Enamel Traces of Early Lifetime Events. Cambridge Studies in Biological and Evolutionary Anthropology: Cambridge University Press, 2008. although Mays argued that the width of the hypoplasia bears only an indirect relationship to the duration of the stressor.

Studies of dental enamel hypoplasia are used to study child health. Unlike bone, teeth are not remodeled, so intact enamel can provide a more reliable indicator of past health events. Dental hypoplasias provide an indicator of health status during the time in childhood when the enamel of the tooth crown is forming. The presence, frequency, and severity of enamel hypoplasia (EH) offer valuable information about general health conditions and the occurrence of disease or malnutrition. Elevated rates of EH are often interpreted as evidence of widespread physiological stress, such as famine, infectious disease outbreaks, or prolonged nutritional deficiencies.{{Cite journal |last1=Disha |first1=Valbona |last2=Zaimi |first2=Marin |last3=Petrela |first3=Elizana |last4=Aliaj |first4=Fatbardha |date=2024-04-15 |title=An Investigation into the Prevalence of Enamel Hypoplasia in an Urban Area Based on the Types and Affected Teeth |journal=Children (Basel, Switzerland) |volume=11 |issue=4 |pages=474 |doi=10.3390/children11040474 |doi-access=free |issn=2227-9067 |pmc=11049504 |pmid=38671691}}{{Cite journal |last1=Armelagos |first1=George J. |last2=Goodman |first2=Alan |last3=Harper |first3=Kristin |last4=Blakey |first4=Michael |date=2009 |title=Enamel hypoplasias and early mortality: Bioarchaeological support for the Barker Hypothesis |url=https://www.researchgate.net/publication/305430518 |journal=Evolutionary Anthropology Issues News and Reviews |volume=18 |issue=6 |pages=261–271|doi=10.1002/evan.20239 }} By comparing the prevalence of dental stress indicators across various groups such as different social classes, geographic regions, or time periods bioarcheologists can also infer disparities in living conditions, access to resources, and overall health.{{Cite journal |last1=Folayan |first1=Morenike Oluwatoyin |last2=Chukwumah |first2=Nneka Maureen |last3=Popoola |first3=Bamidele Olubukola |last4=Temilola |first4=Dada Oluwaseyi |last5=Onyejaka |first5=Nneka Kate |last6=Oyedele |first6=Titus Ayo |last7=Lawal |first7=Folake Barakat |date=2018-09-27 |title=Developmental defects of the enamel and its impact on the oral health quality of life of children resident in Southwest Nigeria |journal=BMC Oral Health |volume=18 |issue=1 |pages=160 |doi=10.1186/s12903-018-0622-3 |doi-access=free |issn=1472-6831 |pmc=6161335 |pmid=30261858}}{{Cite journal |last1=Kanchan |first1=Tanuj |last2=Machado |first2=Meghna |last3=Rao |first3=Ashwin |last4=Krishan |first4=Kewal |last5=Garg |first5=Arun K. |date=2015 |title=Enamel hypoplasia and its role in identification of individuals: A review of literature |journal=Indian Journal of Dentistry |volume=6 |issue=2 |pages=99–102 |doi=10.4103/0975-962X.155887 |doi-access=free |issn=0975-962X |pmc=4455163 |pmid=26097340}}

Not all enamel layers are visible on the tooth surface because enamel layers that are formed early in crown development are buried by later layers. Hypoplasias on this part of the tooth do not show on the tooth surface. Because of this buried enamel, teeth record stressors form a few months after the start of the event. The proportion of enamel crown formation time represented by this buried enamel varies from up to 50 percent in molars to 15-20 percent in anterior teeth. Surface hypoplasias record stressors occur from about one to seven years, or up to 13 years if the third molar is included.Mays, Simon. The Archaeology of Human Bones. 1998. Second ed. New York: Routledge, 2010.

It was long assumed that iron deficiency anemia has marked effects on the flat bones of the cranium of infants and young children. That as the body attempts to compensate for low iron levels by increasing red blood cell production in the young, sieve-like lesions develop in the cranial vaults (termed porotic hyperostosis) and/or the orbits (termed cribra orbitalia). This bone is spongy and soft.Martin, Debra L., Ryan P. Harrod, and Ventura R. Pérez. Bioarchaeology: An Integrated Approach to Working with Human Remains. New York: Springer, 2013.

It is however, unlikely that iron deficiency anemia is a cause of either porotic hyperostosis or cribra orbitalia.Walker et al. 2009 "The Causes of Porotic Hyperostosis and Cribra Orbitalia: A Reappraisal of the Iron-Deficiency-Anemia Hypothesis" American Journal of Physical Anthropology. These are more likely the result of vascular activity in these areas and are unlikely to be pathological. The development of cribra orbitalia and porotic hyperostosis could also be attributed to other causes besides a dietary iron deficiency, such as nutrients lost to intestinal parasites. However, dietary deficiencies are the most probable cause.Schutkowski, Holger. "Thoughts for Food: Evidence and Meaning of Past Dietary Habits." Between Biology and Culture. Ed. Holger Schutkowski. Cambridge Studies in Biological and Evolutionary Anthropology: Cambridge University Press, 2008. 141–64.

Anemia incidence may be a result of inequalities within society, and/or indicative of different work patterns and activities among different groups within society. A study of iron-deficiency among early Mongolian nomads showed that although overall rates of cribra orbitalia declined from 28.7 percent (27.8 percent of the total female population, 28.4 percent of the total male population, 75 percent of the total juvenile population) during the Bronze and Iron Ages, to 15.5 percent during the Hunnu (2209–1907 BP) period, the rate of females with cribra orbitalia remained roughly the same, while incidence among males and children declined (29.4 percent of the total female population, 5.3 percent of the total male population, and 25 percent of the juvenile population had cribra orbitalia). This study hypothesized that adults may have lower rates of cribra orbitalia than juveniles because lesions either heal with age or lead to death. Higher rates of cribia orbitalia among females may indicate lesser health status, or greater survival of young females with cribia orbitalia into adulthood.Bazarsad, Naran. "Iron-Deficiency Anemia in Early Mongolian Nomads." Ancient Health: Skeletal Indicators of Agricultural and Economic Intensification. Eds. Mark Nathan Cohen and Gillian M.M. Crane-Kramer. Gainesville/Tallahassee/Tampa/Boca Raton: University Press of Florida, 2007. 250–54.

Harris lines form before adulthood, when bone growth is temporarily halted or slowed down due to some sort of stress (typically disease or malnutrition).{{Cite journal|last1=Beom|first1=Jaewon|last2=Woo|first2=Eun Jin|last3=Lee|first3=In Sun|last4=Kim|first4=Myeung Ju|last5=Kim|first5=Yi-Suk|last6=Oh|first6=Chang Seok|last7=Lee|first7=Sang-Seob|last8=Lim|first8=Sang Beom|last9=Shin|first9=Dong Hoon|date=2014|title=Harris lines observed in human skeletons of Joseon Dynasty, Korea|journal=Anatomy & Cell Biology|language=en|volume=47|issue=1|pages=66–72|doi=10.5115/acb.2014.47.1.66|issn=2093-3665|pmc=3968268|pmid=24693484}} During this time, bone mineralization continues, but growth does not, or does so at reduced levels. If and when the stressor is overcome, bone growth resumes, resulting in a line of increased mineral density visible in a radiograph. Absent removal of the stressor, no line forms.{{cite journal|last1=Danforth|first1=Marie Elaine|year=1999|title=Nutrition and Politics in Prehistory|journal=Annual Review of Anthropology|volume=28|pages=1–25|doi=10.1146/annurev.anthro.28.1.1}}

Particularly, deficiencies in protein and vitamins, which lead to delayed longitudinal bone growth, can result in the formation of Harris lines.{{Cite journal |last1=Robb |first1=J. |last2=Bigazzi |first2=R. |last3=Lazzarini |first3=L. |last4=Scarsini |first4=C. |last5=Sonego |first5=F. |date=2001 |title=Social "status" and biological "status": a comparison of grave goods and skeletal indicators from Pontecagnano |url=https://pubmed.ncbi.nlm.nih.gov/11424073/ |journal=American Journal of Physical Anthropology |volume=115 |issue=3 |pages=213–222 |doi=10.1002/ajpa.1076 |issn=0002-9483 |pmid=11424073}} During the process of endochondral bone growth, the cessation of osteoblastic activity results in the deposition of a thin layer of bone beneath the cartilage cap, potentially forming Harris lines.{{Cite journal |last=Wolbach |first=S. B. |date=1947 |title=Vitamin-A deficiency and excess in relation to skeletal growth |url=https://pubmed.ncbi.nlm.nih.gov/20284696/ |journal=The Journal of Bone and Joint Surgery. American Volume |volume=29 |issue=1 |pages=171–192 |issn=0021-9355 |pmid=20284696}}{{Cite journal |last1=Platt |first1=B. S. |last2=Stewart |first2=R. J. |date=1962 |title=Transverse trabeculae and osteoporosis in bones in experimental protein-calorie deficiency |url=https://pubmed.ncbi.nlm.nih.gov/13943979/ |journal=The British Journal of Nutrition |volume=16 |pages=483–495 |doi=10.1079/bjn19620047 |issn=0007-1145 |pmid=13943979}} Subsequent recovery, necessary for the restoration of osteoblastic activity, is also implicated in Harris line formation.{{Cite journal |last=Seres |first=David S. |date=2005 |title=Surrogate nutrition markers, malnutrition, and adequacy of nutrition support |url=https://pubmed.ncbi.nlm.nih.gov/16207668/ |journal=Nutrition in Clinical Practice|volume=20 |issue=3 |pages=308–313 |doi=10.1177/0115426505020003308 |issn=0884-5336 |pmid=16207668}} When matured cartilage cells reactivate, bone growth resumes, thickening the bony stratum. Therefore, complete recovery from periods of chronic illness or malnutrition manifests as transverse lines on radiographs. Lines tend to be thicker with prolonged and severe malnutrition. Harris line formation typically peaks in long bones around 2–3 years after birth and becomes rare after the age of 5 until adulthood. Harris lines occur more frequently in boys than in girls.{{Cite journal |last1=Hughes |first1=C. |last2=Heylings |first2=D. J. |last3=Power |first3=C. |date=1996 |title=Transverse (Harris) lines in Irish archaeological remains |url=https://pubmed.ncbi.nlm.nih.gov/8876817/ |journal=American Journal of Physical Anthropology |volume=101 |issue=1 |pages=115–131 |doi=10.1002/(SICI)1096-8644(199609)101:1<115::AID-AJPA8>3.0.CO;2-U |issn=0002-9483 |pmid=8876817}}

The stress hormone cortisol is deposited in hair as it grows. This has been used successfully to detect fluctuating levels of stress in the later lifespan of mummies.{{cite web|url=http://www.schulich.uwo.ca/moleculartoxicology/files/Linked/Hair%20cortisol%20as%20a%20biological%20marker%20of%20chronic%20stress%20Current%20status%20future%20directions%20and%20unanswered%20questions.pdf|title=Hair cortisol as a biological marker of chronic stress: Current status, future directions and unanswered questions|date=2011-09-12|access-date=2012-05-25}}

Examining the effects that activities has upon the skeleton allows the archaeologist to examine who was doing what kinds of labor, and how activities were structured within society. Labor within the household may be divided according to gender and age, or be based on other social structures. Human remains can allow archaeologists to uncover these patterns.

Living bones are subject to Wolff's law, which states that bones are physically affected and remodeled by physical activity or inactivity.{{cite journal|last1=Wolff|first1=Julius|year=1893|title=Review: Das Gesetz Der Transformation Der Knochen (the Law of the Transformation of Bones)|journal=The British Medical Journal|volume=1|issue=1673|page=124}} Increases in mechanical stress tend to produce thicker and stronger bones. Disruptions in homeostasis caused by nutritional deficiency or disease{{cite journal|last1=Robbins Schug|first1=Gwen|last2=Goldman|first2=Haviva M.|title=Birth is but our death begun: a bioarchaeological assessment of skeletal emaciation in immature human skeletons in the context of environmental, social, and subsistence transition|journal=American Journal of Physical Anthropology|volume=155|issue=2|pages=243–259|doi=10.1002/ajpa.22536|pmid=24839102|year=2014|s2cid=39512115 |url=http://libres.uncg.edu/ir/uncg/f/G_Robbins_Schug_Birth_2014.pdf}} or profound inactivity/disuse/disability can lead to bone loss.{{cite journal|last1=Scott|first1=J.H.|year=1957|title=Muscle Growth and Function in Relation to Skeletal Morphology|journal=American Journal of Physical Anthropology|volume=15|issue=2|pages=197–234|doi=10.1002/ajpa.1330150210|pmid=13470043}} While the acquisition of bipedal locomotion and body mass appear to determine the size and shape of children's bones,{{cite journal|last1=Ruff|first1=C.B.|year=2007|title=Body Size Prediction from Juvenile Skeletal Remains|journal=American Journal of Physical Anthropology|volume=133|issue=1|pages=698–716|doi=10.1002/ajpa.20568|pmid=17295297}}{{cite journal|last1=Robbins|first1=Gwen|last2=Sciulli|first2=Paul|last3=Blatt|first3=Samantha|year=2010|title=Estimating Body Mass in Subadult Human Skeletons|journal=American Journal of Physical Anthropology|volume=143|issue=1|pages=146–150|doi=10.1002/ajpa.21320|pmid=20734440|url=http://libres.uncg.edu/ir/uncg/f/G_Robbins_Schug_Estimating_2010.pdf}}{{cite journal|last1=Schug|first1=Robbins|last2=Gwen|last3=Gupta|first3=Sat|last4=Cowgill|first4=Libby W.|last5=Sciulli|first5=Paul|last6=Blatt|first6=Samantha|year=2013|title=Panel regression formulas for stature and body mass estimation in immature human skeletons, without reference to specific age estimates|journal=Journal of Archaeological Science|volume=40|issue=7|pages=3076–3086|doi=10.1016/j.jas.2013.02.025}} activity during the adolescent growth period seems to exert a greater influence on the size and shape of adult bones than exercise later in life.{{cite journal|last1=Pearson|first1=Osbjorn M.|last2=Lieberman|first2=Daniel E.|year=2004|title=The Aging of Wolff's "Law:" Ontogeny and Response to Mechanical Loading in Cortical Bone|journal=Yearbook of Physical Anthropology|volume=47|pages=63–99|doi=10.1002/ajpa.20155|pmid=15605390|doi-access=free}}

Muscle attachment sites (entheses) have been thought to be impacted in the same way, causing entheseal changes.{{Cite web|url=http://www.uc.pt/en/cia/msm/MSM_terminology3.pdf|title=Terminology. Entheses in medical literature and physical anthropology: a brief review|last1=Jurmain|first1=R|last2=Villotte|first2=S}}{{Cite journal|last1=Villotte|first1=Sébastien|last2=Assis|first2=Sandra|last3=Cardoso|first3=Francisca Alves|last4=Henderson|first4=Charlotte Yvette|last5=Mariotti|first5=Valentina|last6=Milella|first6=Marco|last7=Pany-Kucera|first7=Doris|last8=Speith|first8=Nivien|last9=Wilczak|first9=Cynthia A.|date=2016-06-01|title=In search of consensus: Terminology for entheseal changes (EC)|journal=International Journal of Paleopathology|volume=13|pages=49–55|doi=10.1016/j.ijpp.2016.01.003|pmid=29539508|bibcode=2016IJPal..13...49V |hdl=10316/44443 |s2cid=3902457 |url=http://eprints.bournemouth.ac.uk/24390/1/IJPP-D-15-00084R2.pdf}} These changes were widely used to study activity-patterns,{{Cite book|title=A Companion to Paleopathology|last1=Jurmain|first1=Robert|last2=Cardoso|first2=Francisca Alves|last3=Henderson|first3=Charlotte|last4=Villotte|first4=Sébastien|date=2011-01-01|publisher=Wiley-Blackwell|isbn=9781444345940|editor-last=Grauer|editor-first=Anne L.|pages=531–552|language=en|doi=10.1002/9781444345940.ch29}} but research has shown that processes associated with aging have a greater impact than occupational stresses.{{Cite journal|title=What can bones tell about labour and occupation : the analysis of skeletal markers of occupational stress in the Identified Skeletal Collection of the Anthropological Museum of the University of Coimbra (preliminary results)|url=http://cat.inist.fr/?aModele=afficheN&cpsidt=3293764|journal=Antropologia Portuguesa|volume=13|issn=0870-0990|access-date=2016-09-05|archive-date=2017-09-15|archive-url=https://web.archive.org/web/20170915204924/http://cat.inist.fr/?aModele=afficheN&cpsidt=3293764|url-status=dead}}{{Cite journal|last1=Alves Cardoso|first1=F.|last2=Henderson|first2=C. Y.|date=2010-04-01|title=Enthesopathy formation in the humerus: Data from known age-at-death and known occupation skeletal collections|journal=American Journal of Physical Anthropology|volume=141|issue=4|pages=550–560|doi=10.1002/ajpa.21171|issn=1096-8644|pmid=19927279}}{{Cite journal|last1=Alves Cardoso|first1=F.|last2=Henderson|first2=C.|date=2013-03-01|title=The Categorisation of Occupation in Identified Skeletal Collections: A Source of Bias?|journal=International Journal of Osteoarchaeology|language=en|volume=23|issue=2|pages=186–196|doi=10.1002/oa.2285|issn=1099-1212|hdl=10316/21142|url=https://estudogeral.sib.uc.pt/bitstream/10316/21142/1/OA-12-0105.R1%20Alves%20Cardoso%20%26%20Hend.pdf|hdl-access=free}}{{Cite journal|last1=Milella|first1=Marco|last2=Giovanna Belcastro|first2=Maria|last3=Zollikofer|first3=Christoph P. E.|last4=Mariotti|first4=Valentina|date=2012-07-01|title=The effect of age, sex, and physical activity on entheseal morphology in a contemporary Italian skeletal collection|journal=American Journal of Physical Anthropology|volume=148|issue=3|pages=379–388|doi=10.1002/ajpa.22060|issn=1096-8644|pmid=22460619}}{{Cite journal|last1=Henderson|first1=C. Y.|last2=Nikita|first2=E.|date=2015-06-30|title=Accounting for multiple effects and the problem of small sample sizes in osteology: a case study focussing on entheseal changes|journal=Archaeological and Anthropological Sciences|volume=8|issue=4|language=en|pages=805–817|doi=10.1007/s12520-015-0256-1|issn=1866-9557|hdl=10316/44428|s2cid=83293108|hdl-access=free}}{{Cite journal|last1=Henderson|first1=C. Y.|last2=Mariotti|first2=V.|last3=Santos|first3=F.|last4=Villotte|first4=S.|last5=Wilczak|first5=C. A.|date=2017-06-20|title=The new Coimbra method for recording entheseal changes and the effect of age-at-death|journal=BMSAP|volume=29|issue=3–4|language=en|pages=140–149|doi=10.1007/s13219-017-0185-x|doi-broken-date=16 January 2025 |issn=0037-8984|hdl=10316/44430|s2cid=29420179|url=https://estudogeral.sib.uc.pt//bitstream/10316/44430/1/Age%20effects%20Methodology%20group%20paper%20accepted%20version.pdf|hdl-access=free}} It has also been shown that geometric changes to bone structure (described above) and entheseal changes differ in their underlying cause with the latter little affected by occupation.{{Cite journal|last1=Michopoulou|first1=E.|last2=Nikita|first2=E.|last3=Henderson|first3=C. Y.|date=2016-01-01|title=A test of the effectiveness of the Coimbra method in capturing activity-induced entheseal changes|journal=International Journal of Osteoarchaeology|volume=27|issue=3|language=en|pages=409–417|doi=10.1002/oa.2564|issn=1099-1212}}{{Cite journal|last1=Michopoulou|first1=Efrossyni|last2=Nikita|first2=Efthymia|last3=Valakos|first3=Efstratios D.|date=2015-12-01|title=Evaluating the efficiency of different recording protocols for entheseal changes in regards to expressing activity patterns using archival data and cross-sectional geometric properties|journal=American Journal of Physical Anthropology|language=en|volume=158|issue=4|pages=557–568|doi=10.1002/ajpa.22822|issn=1096-8644|pmid=26239396|doi-access=free}} Joint changes, including osteoarthritis, have been used to infer occupations, but in general these are also manifestations of the aging process.

Markers of occupational stress, which include morphological changes to the skeleton and dentition as well as joint changes at specific locations have been widely used to infer specific (rather than general) activities.{{Cite book|title=Atlas of occupational markers on human remains|last1=Capasso|first1=L|last2=Kennedy|first2=K.A.R.|last3=Wilczak|first3=C|publisher=Edigrafital|year=1999}} Such markers are often based on single cases described in late nineteenth century clinical literature.{{Cite journal|last=Lane|first=W. Arbuthnot|date=1888-07-01|title=Anatomy and Physiology of the Shoemaker|journal=Journal of Anatomy and Physiology|volume=22|issue=Pt 4|pages=592.1–628|pmc=1288729|pmid=17231765}} One such marker has been found to be a reliable indicator of lifestyle: the external auditory exostosis also called surfer's ear, which is a small bony protuberance in the ear canal that occurs in those working in proximity to cold water.{{Cite journal|last=Kennedy|first=G. E.|date=1986-12-01|title=The relationship between auditory exostoses and cold water: A latitudinal analysis|journal=American Journal of Physical Anthropology|language=en|volume=71|issue=4|pages=401–415|doi=10.1002/ajpa.1330710403|issn=1096-8644|pmid=3812656}}{{Cite journal|last1=Villotte|first1=Sébastien|last2=Knüsel|first2=Christopher J.|date=2016-04-01|title=External auditory exostoses and prehistoric aquatic resource procurement|journal=Journal of Archaeological Science: Reports|volume=6|pages=633–636|doi=10.1016/j.jasrep.2015.05.013|bibcode=2016JArSR...6..633V |s2cid=127016744 |url=https://hal.archives-ouvertes.fr/hal-03146975/file/2016%20Villotte%20Knusel%20EAE%20auteur.pdf}}

One example of how these changes have been used to study activities is the New York African Burial Ground in New York. This provides evidence of the brutal working conditions under which the enslaved labored;Wilczak, C., R.C. Watkins, and M.L. Blakey. Skeletal Indicators of Work: Musculoskeletal, Arthritic, and Traumatic Events: US Department of the Interior, National Park Service, 2004. osteoarthritis of the vertebrae was common even among the young. The pattern of osteoarthritis combined with the early age of onset provides evidence of labor that resulted in mechanical strain to the neck. One male skeleton shows stress lesions at 37 percent of 33 muscle or ligament attachments, showing he experienced significant musculoskeletal stress. Overall, the interred show signs of significant musculoskeletal stress and heavy workloads, although workload and activities varied by individual. Some show high levels of stress, while others do not. This indicates the variety of types of labor (e.g., domestic vs. carrying heavy loads) labor.

Fractures to bones during or after excavation appear relatively fresh, with broken surfaces appearing white and unweathered. Distinguishing between fractures around the time of death and post-depositional fractures in bone is difficult, as both types of fractures show signs of weathering. Unless evidence of bone healing or other factors are present, researchers may choose to regard all weathered fractures as post-depositional.

Evidence of perimortal fractures (or fractures inflicted on a fresh corpse) can be distinguished in unhealed metal blade injuries to the bones. Living or freshly dead bones are somewhat resilient, so metal blade injuries to bone generate a linear cut with relatively clean edges rather than irregular shattering. Archaeologists have attempted to use the microscopic parallel scratch marks on cut bones in order to estimate the trajectory of the blade that caused the injury.Wenham, S.J., and J. Wakely. Features of Blade-Injuries to Bone Surfaces in Six Anglo-Saxon Skeletons from Eccles, Kent: BAR 211 Oxford, 1989.

Dental caries are caused by localized destruction of tooth enamel, as a result of acids produced by bacteria feeding upon and fermenting carbohydrates in the mouth.{{Cite journal|last=Loesche|first=W.J.|date=November 1988|title=The Role of Spirochetes in Periodontal Disease|url=http://dx.doi.org/10.1177/08959374880020021201|journal=Advances in Dental Research|volume=2|issue=2|pages=275–283|doi=10.1177/08959374880020021201|pmid=3271022|issn=0895-9374|hdl=2027.42/68092|s2cid=13175233|hdl-access=free}} Agriculture is strongly associated with a higher rate of caries than foraging, because of the associated higher levels of carbohydrates produced by agriculture. For example, bioarchaeologists have used caries in skeletons to correlate a diet of rice with disease.{{cite journal|last1=Tayles|first1=N.|last2=Domett|first2=K.|last3=Nelsen|first3=K.|year=2000|title=Agriculture and dental caries? The case of rice in prehistoric Southeast Asia|journal=World Archaeology|volume=32|issue=1|pages=68–83|doi=10.1080/004382400409899|pmid=16475298|s2cid=43087099}} Women may be more vulnerable to caries compared to men due to having lower saliva flow, the positive correlation of estrogen with increased caries rates, and because of pregnancy-associated physiological changes, such as suppression of the immune system and a possible concomitant decrease in antimicrobial activity in the oral cavity.{{cite journal|last1=Lukacs|first1=John R|year=2008|title=Fertility and Agriculture Accentuate Sex Differences in Dental Caries Rates|journal=Current Anthropology|volume=49|issue=5|pages=901–14|doi=10.1086/592111|s2cid=146568976|url=https://zenodo.org/record/995655}}

File:Isotopesandbioarch bargraph.pngStable isotope biogeochemistry uses variations in isotopic signatures and relates them to biogeochemical processes. The science is based on the preferential fractionation of lighter or heavier isotopes, which results in enriched and depleted isotopic signatures compared to a standard value. Essential elements for life such as carbon, nitrogen, oxygen, and sulfur are the primary stable isotope systems used to interrogate archeological discoveries. Isotopic signatures from multiple systems are typically used in tandem to create a comprehensive understanding of the analyzed material. These systems are most commonly used to trace the geographic origin of archaeological remains and investigate the diets, mobility, and cultural practices of ancient humans.{{Cite book |title=Isoscapes : understanding movement, pattern, and process on earth through isotope mapping |date=2010 |publisher=Springer |others=Jason B. West |isbn=978-90-481-3354-3 |location=Dordrecht |oclc=567359262}}{{Cite journal |last=Britton |first=Kate |date=August 2017 |title=A stable relationship: isotopes and bioarchaeology are in it for the long haul |url=https://www.cambridge.org/core/journals/antiquity/article/abs/stable-relationship-isotopes-and-bioarchaeology-are-in-it-for-the-long-haul/4EF9D9B756516509C59CE52C086BFF64 |journal=Antiquity |language=en |volume=91 |issue=358 |pages=853–864 |doi=10.15184/aqy.2017.98 |hdl=2164/8892 |s2cid=164265353 |issn=0003-598X|hdl-access=free |url-access=subscription }}

Stable isotope analysis of carbon in human bone collagen allows bioarchaeologists to carry out dietary reconstruction and to make nutritional inferences. These chemical signatures reflect long-term dietary patterns, rather than a single meal or feast. Isotope ratios in food, especially plant food, are directly and predictably reflected in bone chemistry,{{Citation |last1=Burton |first1=James H. |title=The Use and Abuse of Trace Elements for Paleodietary Research |url=http://dx.doi.org/10.1007/0-306-47194-9_8 |pages=159–171 |place=Boston |publisher=Kluwer Academic Publishers |isbn=0-306-46457-8 |access-date=2022-05-25 |last2=Price |first2=T. Douglas|series=Advances in Archaeological and Museum Science |year=2002 |volume=5 |doi=10.1007/0-306-47194-9_8 |url-access=subscription }} allowing researchers to partially reconstruct recent diet using stable isotopes as tracers.{{Cite journal |last=Klepinger |first=L L |date=1984-10-01 |title=Nutritional Assessment From Bone |url=https://www.annualreviews.org/doi/10.1146/annurev.an.13.100184.000451 |journal=Annual Review of Anthropology |volume=13 |issue=1 |pages=75–96 |doi=10.1146/annurev.an.13.100184.000451 |issn=0084-6570|url-access=subscription }}{{Cite journal |last=Storey |first=Rebecca |date=July 1986 |title=Paleopathology at the Origins of Agriculture. Mark Nathan Cohen and George J. Armelagos, editors. Academic Press, Inc., Orlando, 1984. xx + 615 pp., figures, tables, references, index. $59.00 (cloth). |url=http://dx.doi.org/10.2307/281762 |journal=American Antiquity |volume=51 |issue=3 |pages=661–662 |doi=10.2307/281762 |jstor=281762 |s2cid=165005577 |issn=0002-7316|url-access=subscription }} Stable isotope analysis monitors the ratio of carbon 13 to carbon 12 (¹³C/¹²C), which is expressed as parts per thousand using delta notation (δ¹³C).{{Cite web |title=Bone Chemistry |url=http://luna.cas.usf.edu/~rtykot/Bone.html |access-date=2022-05-25 |website=luna.cas.usf.edu}} The ¹³C and ¹²C ratio is either depleted (more negative) or enriched (more positive) relative to a standard.{{Cite journal |last1=Rounick |first1=J. S. |last2=Winterbourn |first2=M. J. |date=1986-03-01 |title=Stable Carbon Isotopes and Carbon Flow in Ecosystems: Measuring 13C to 12C ratios can help trace carbon pathways |url=https://doi.org/10.2307/1310304 |journal=BioScience |volume=36 |issue=3 |pages=171–177 |doi=10.2307/1310304 |jstor=1310304 |issn=0006-3568|url-access=subscription }} ¹²C and ¹³C occur in a ratio of approximately 98.9 to 1.1.

File:JMueller CarbonDiagramV2.jpg

The ratio of carbon isotopes in humans varies according to the types of plants digested with different photosynthesis pathways. The three photosynthesis pathways are C3 carbon fixation, C4 carbon fixation and Crassulacean acid metabolism. C4 plants are mainly grasses from tropical and subtropical regions, and are adapted to higher levels of radiation than C3 plants. Corn, millet{{Cite journal |last=Larsen |first=Clark Spencer |date=1995-10-01 |title=Biological Changes in Human Populations with Agriculture |url=https://www.annualreviews.org/doi/10.1146/annurev.an.24.100195.001153 |journal=Annual Review of Anthropology |volume=24 |issue=1 |pages=185–213 |doi=10.1146/annurev.an.24.100195.001153 |issn=0084-6570|url-access=subscription }} and sugar cane are some well-known C4 crops, while trees and shrubs use the C3 pathway.{{Citation |last=Bocherens |first=Hervé |title=Biogeochemical Approaches to Paleodietary Analysis |chapter=Preservation of Isotopic Signals (13C, 15N)_in Pleistocene Mammals |chapter-url=http://dx.doi.org/10.1007/0-306-47194-9_4 |series=Advances in Archaeological and Museum Science |year=2002 |volume=5 |pages=65–88 |place=Boston |publisher=Kluwer Academic Publishers |doi=10.1007/0-306-47194-9_4 |isbn=0-306-46457-8 |access-date=2022-05-25}} C4 carbon fixation is more efficient when temperatures are high and atmospheric CO₂ concentrations are low.{{Citation |last1=Ehleringer |first1=James R. |title=Photosynthetic Pathways and Climate |date=2001 |url=http://dx.doi.org/10.1016/b978-012631260-7/50023-6 |work=Global Biogeochemical Cycles in the Climate System |pages=267–277 |publisher=Elsevier |access-date=2022-05-24 |last2=Cerling |first2=Thure E.|doi=10.1016/b978-012631260-7/50023-6 |isbn=9780126312607 |url-access=subscription }} C3 plants are more common and numerous than C4 plants as C3 carbon fixation is more efficient in a wider range of temperatures and atmospheric CO₂ concentrations.

The different photosynthesis pathways used by C3 and C4 plants cause them to discriminate differently towards ¹³C leading to distinctly different ranges of δ¹³C. C4 plants range between -9 and -16‰, and C3 plants range between -22 and -34‰. The isotopic signature of consumer collagen is close the δ¹³C of dietary plants, while apatite, a mineral component of bones and teeth, has an ~14‰ offset from dietary plants due fractionation associated with mineral formation. Stable carbon isotopes have been used as tracers of C4 plants in paleodiets. For example, the rapid and dramatic increase in ¹³C in human collagen after the adoption of maize agriculture in North America documents the transition from a C3 to a C4 (native plants to corn) diet by 1300 CE.{{Cite journal |last1=Peterson |first1=Bruce J. |last2=Fry |first2=Brian |date=1987-11-01 |title=Stable isotopes in ecosystem studies |url=https://www.annualreviews.org/doi/10.1146/annurev.es.18.110187.001453 |journal=Annual Review of Ecology and Systematics |volume=18 |issue=1 |pages=293–320 |doi=10.1146/annurev.es.18.110187.001453 |bibcode=1987AnRES..18..293P |issn=0066-4162|url-access=subscription }}{{Cite journal |last1=Van Der Merwe |first1=Nikolaas J. |last2=Vogel |first2=J. C. |date=December 1978 |title=13C Content of human collagen as a measure of prehistoric diet in woodland North America |url=https://www.nature.com/articles/276815a0 |journal=Nature |language=en |volume=276 |issue=5690 |pages=815–816 |doi=10.1038/276815a0 |pmid=364321 |bibcode=1978Natur.276..815V |s2cid=4309016 |issn=1476-4687|url-access=subscription }}

Skeletons excavated from the Coburn Street Burial Ground (1750 to 1827 CE) in Cape Town, South Africa, were analyzed using stable isotope data in order to determine geographical histories and life histories.{{Cite journal |last1=Cox |first1=Glenda |last2=Sealy |first2=Judith |last3=Schrire |first3=Carmel |last4=Morris |first4=Alan |date=2001-01-01 |title=Stable carbon and nitrogen isotopic analyses of the underclass at the colonial Cape of Good Hope in the eighteenth and nineteenth centuries |url=https://doi.org/10.1080/00438240126647 |journal=World Archaeology |volume=33 |issue=1 |pages=73–97 |doi=10.1080/00438240126647 |issn=0043-8243 |pmid=16475301|s2cid=12440830 |url-access=subscription }} The people buried in this cemetery were assumed to be slaves and members of the underclass based on the informal nature of the cemetery; biomechanical stress analysis{{Cite journal |last1=Ledger |first1=Michael |last2=Holtzhausen |first2=Lucy-May |last3=Constant |first3=Deborah |last4=Morris |first4=Alan G. |date=2000 |title=Biomechanical beam analysis of long bones from a late 18th century slave cemetery in Cape Town, South Africa |url=http://dx.doi.org/10.1002/(sici)1096-8644(2000)112:2<207::aid-ajpa7>3.0.co;2-k |journal=American Journal of Physical Anthropology |volume=112 |issue=2 |pages=207–216 |doi=10.1002/(sici)1096-8644(2000)112:2<207::aid-ajpa7>3.0.co;2-k |pmid=10813703 |issn=0002-9483|url-access=subscription }} and stable isotope analysis, combined with other archaeological data, seem to support this supposition.

Based on stable isotope levels, one study reported that eight Cobern Street Burial Ground individuals consumed a diet based on C4 (tropical) plants in childhood, then consumed more C3 plants, which were more common there later in their lives. Six of these individuals had dental modifications similar to those carried out by peoples inhabiting tropical areas known to be targeted by slavers who brought enslaved individuals from other parts of Africa to the colony. Based on this evidence, it was argued that these individuals represent enslaved persons from areas of Africa where C4 plants were consumed and who were brought to the Cape as laborers. These individuals were not assigned to a specific ethnicity, but similar dental modifications are carried out by the Makua, Yao, and Marav peoples. Four individuals were buried with no grave goods, in accordance with Muslim tradition, facing Signal Hill, which is a point of significance for local Muslims. Their isotopic signatures indicate that they grew up in a temperate environment consuming mostly C3 plants, but some C4 The study argued that these individuals were from the Indian Ocean area. It also suggested that these individuals were Muslims. It argued that stable isotopic analysis of burials, combined with historical and archaeological data were an effective way of investigating the migrations forced by the African Slave Trade, as well as the emergence of the underclass and working class in the Old World.

The nitrogen stable isotope system is based on the relative enrichment/depletion of ¹⁵N in comparison to ¹⁴N in δ15N. Carbon and nitrogen stable isotope analyses are complementary in paleodiet studies. Nitrogen isotopes in bone collagen are ultimately derived from dietary protein, while carbon can be contributed by protein, carbohydrate, or fat.{{Cite journal |last1=Sillen |first1=Andrew |last2=Sealy |first2=Judith C. |last3=Merwe |first3=Nikolaas J. van der |date=July 1989 |title=Chemistry and Paleodietary Research: No More Easy Answers |url=https://www.cambridge.org/core/journals/american-antiquity/article/abs/chemistry-and-paleodietary-research-no-more-easy-answers/9F8F4F81FFA5883EB04BA052D62E3A12 |journal=American Antiquity |language=en |volume=54 |issue=3 |pages=504–512 |doi=10.2307/280778 |jstor=280778 |s2cid=95529499 |issn=0002-7316|url-access=subscription }} δ¹³C values help distinguish between dietary protein and plant sources while systematic increases in δ¹⁵N values as you move up in trophic level helps determine the position of protein sources in the food web.{{Cite journal |last1=Müldner |first1=Gundula |last2=Montgomery |first2=Janet |last3=Cook |first3=Gordon |last4=Ellam |first4=Rob |last5=Gledhill |first5=Andrew |last6=Lowe |first6=Chris |date=December 2009 |title=Isotopes and individuals: diet and mobility among the medieval Bishops of Whithorn |url=https://www.cambridge.org/core/journals/antiquity/article/abs/isotopes-and-individuals-diet-and-mobility-among-the-medieval-bishops-of-whithorn/63C1015684A03158CA63A144A5B4F0B3 |journal=Antiquity |language=en |volume=83 |issue=322 |pages=1119–1133 |doi=10.1017/S0003598X00099403 |s2cid=73690846 |issn=0003-598X}}{{Cite journal |last1=Hedges |first1=Robert E. M. |last2=Reynard |first2=Linda M. |date=2007-08-01 |title=Nitrogen isotopes and the trophic level of humans in archaeology |url=https://www.sciencedirect.com/science/article/pii/S0305440306002214 |journal=Journal of Archaeological Science |language=en |volume=34 |issue=8 |pages=1240–1251 |doi=10.1016/j.jas.2006.10.015 |bibcode=2007JArSc..34.1240H |issn=0305-4403|url-access=subscription }} ¹⁵N increases 3-4% with each trophic step upward.{{Cite journal |last1=White |first1=Christine D. |last2=Pendergast |first2=David M. |last3=Longstaffe |first3=Fred J. |last4=Law |first4=Kimberley R. |date=December 2001 |title=Social Complexity and Food Systems at Altun Ha, Belize: The Isotopic Evidence |url=https://www.cambridge.org/core/journals/latin-american-antiquity/article/abs/social-complexity-and-food-systems-at-altun-ha-belize-the-isotopic-evidence/63F8B0C1D4B354A3A70E4CC874C3C2B5 |journal=Latin American Antiquity |language=en |volume=12 |issue=4 |pages=371–393 |doi=10.2307/972085 |jstor=972085 |s2cid=163750191 |issn=1045-6635|url-access=subscription }}{{Cite journal |last1=Katzenberg |first1=M. Anne |last2=Schwarcz |first2=Henry P. |last3=Knyf |first3=Martin |last4=Melbye |first4=F. Jerome |date=April 1995 |title=Stable Isotope Evidence for Maize Horticulture and Paleodiet in Southern Ontario, Canada |url=https://www.cambridge.org/core/journals/american-antiquity/article/abs/stable-isotope-evidence-for-maize-horticulture-and-paleodiet-in-southern-ontario-canada/57E41CA50B96E0E2A6F1C1C729C121E3 |journal=American Antiquity |language=en |volume=60 |issue=2 |pages=335–350 |doi=10.2307/282144 |jstor=282144 |s2cid=164171238 |issn=0002-7316|url-access=subscription }} It has been suggested that the relative difference between human δ¹⁵N values and animal protein values scales with the proportion of that animal protein in the diet,{{Cite journal |last1=Prowse |first1=Tracy |last2=Schwarcz |first2=Henry P. |last3=Saunders |first3=Shelley |last4=Macchiarelli |first4=Roberto |last5=Bondioli |first5=Luca |date=2004-03-01 |title=Isotopic paleodiet studies of skeletons from the Imperial Roman-age cemetery of Isola Sacra, Rome, Italy |url=https://www.sciencedirect.com/science/article/pii/S0305440303001183 |journal=Journal of Archaeological Science |language=en |volume=31 |issue=3 |pages=259–272 |doi=10.1016/j.jas.2003.08.008 |bibcode=2004JArSc..31..259P |issn=0305-4403|url-access=subscription }} though this interpretation has been questioned due to contradictory views on the impact of nitrogen intake through protein consumption and nitrogen loss through waste release on ¹⁵N enrichment in the body.

Variations in nitrogen values within the same trophic level are also considered.{{Cite journal |last1=Szpak |first1=Paul |last2=White |first2=Christine D. |last3=Longstaffe |first3=Fred J. |last4=Millaire |first4=Jean-François |last5=Sánchez |first5=Víctor F. Vásquez |date=2013-01-16 |title=Carbon and Nitrogen Isotopic Survey of Northern Peruvian Plants: Baselines for Paleodietary and Paleoecological Studies |journal=PLOS ONE |language=en |volume=8 |issue=1 |pages=e53763 |doi=10.1371/journal.pone.0053763 |issn=1932-6203 |pmc=3547067 |pmid=23341996|bibcode=2013PLoSO...853763S |doi-access=free }} Nitrogen variations in plants, for example, can be caused by plant-specific reliance on nitrogen gas which causes the plant to mirror atmospheric values. Enriched or higher δ¹⁵N values can be achieved in plants that grew in soil fertilized by animal waste. Nitrogen isotopes have been used to estimate the relative contributions of legumes verses nonlegumes, as well as terrestrial versus marine resources.{{Cite journal |last1=Schwarcz |first1=Henry P. |last2=Schoeninger |first2=Margaret J. |date=1991 |title=Stable isotope analyses in human nutritional ecology |journal=American Journal of Physical Anthropology |language=en |volume=34 |issue=S13 |pages=283–321 |doi=10.1002/ajpa.1330340613|doi-access=free }} While other plants have δ¹⁵N values that range from 2 to 6‰, legumes have lower ¹⁴N/¹⁵N ratios (close to 0‰, i.e. atmospheric N₂) because they can fix molecular nitrogen, rather than having to rely on soil nitrates and nitrites. Therefore, one potential explanation for lower δ¹⁵N values in human remains is an increased consumption of legumes or animals that eat them. ¹⁵N values increase with meat consumption, and decrease with legume consumption. The ¹⁴N/¹⁵N ratio could be used to gauge the contribution of meat and legumes to the diet.

File:JMueller oxygenbioarchaeology.jpg

The oxygen stable isotope system is based on the ¹⁸O/¹⁶O (δ¹⁸O) ratio in a given material, which is enriched/depleted relative to a standard. The field typically normalizes to both Vienna Standard Mean Ocean Water (VSMOW) and Standard Light Antarctic Precipitation (SLAP).{{Cite journal |last1=Pederzani |first1=Sarah |last2=Britton |first2=Kate |date=2019-01-01 |title=Oxygen isotopes in bioarchaeology: Principles and applications, challenges and opportunities |url=https://www.sciencedirect.com/science/article/pii/S0012825218301284 |journal=Earth-Science Reviews |language=en |volume=188 |pages=77–107 |doi=10.1016/j.earscirev.2018.11.005 |bibcode=2019ESRv..188...77P |hdl=2164/13249 |s2cid=133661731 |issn=0012-8252|hdl-access=free }} This system is famous for its use in paleoclimatic studies but it also a prominent source of information in bioarchaeology.

Variations in δ¹⁸O values in skeletal remains are directly related to the isotopic composition of the consumer's body water. isotopic composition of mammalian body water is primarily controlled by consumed water. δ¹⁸O values of freshwater drinking sources vary due to mass fractionations related to mechanisms of the global water cycle.{{Cite book |author=Gat, Joel |title=Isotope hydrology a study of the water cycle |date=2010 |publisher=Imperial College Press |isbn=978-1-84816-474-1 |oclc=1162451349}} Evaporated water vapor is more enriched in ¹⁶O (isotopically lighter; more negative delta value) compared to the remaining water, which is depleted in ¹⁶O (isotopically heavier; more positive delta value). An accepted first-order approximation for the isotopic composition of animal drinking water is local precipitation, though this is complicated to varying degrees by confounding water sources like natural springs or lakes. The baseline δ¹⁸O used in archaeological studies is modified depending on the relevant environmental and historical context.

δ¹⁸O values of bioapatite in human skeletal remains are assumed to have formed in equilibrium with body water, thus providing a species-specific relationship to oxygen isotopic composition of body water.{{Cite journal |last1=Kohn |first1=Matthew J. |last2=Cerling |first2=Thure E. |date=2002-01-01 |title=Stable Isotope Compositions of Biological Apatite |url=https://doi.org/10.2138/rmg.2002.48.12 |journal=Reviews in Mineralogy and Geochemistry |volume=48 |issue=1 |pages=455–488 |doi=10.2138/rmg.2002.48.12 |bibcode=2002RvMG...48..455K |issn=1529-6466|url-access=subscription }} The same cannot be said for human bone collagen, as δ¹⁸O values in collagen seem to be impacted by drinking water, food water, and a combination of metabolic and physiological processes.{{Cite journal |last1=Kirsanow |first1=Karola |last2=Tuross |first2=Noreen |date=2011-09-15 |title=Oxygen and hydrogen isotopes in rodent tissues: Impact of diet, water and ontogeny |url=https://www.sciencedirect.com/science/article/pii/S0031018211001465 |journal=Palaeogeography, Palaeoclimatology, Palaeoecology |series=Special Issue: Fossil bones and teeth: preservation or alteration of biogenic compositions? |language=en |volume=310 |issue=1 |pages=9–16 |doi=10.1016/j.palaeo.2011.03.022 |bibcode=2011PPP...310....9K |issn=0031-0182|url-access=subscription }} δ¹⁸O values from bone minerals are essentially an averaged isotopic signature throughout the entire life of the individual.{{Cite journal |last=Balasse |first=Marie |date=2002 |title=Reconstructing dietary and environmental history from enamel isotopic analysis: time resolution of intra-tooth sequential sampling |journal=International Journal of Osteoarchaeology |volume=12 |issue=3 |pages=155–165 |doi=10.1002/oa.601 |issn=1047-482X|doi-access=free }}

While carbon and nitrogen are used primarily to investigate the diets of ancient humans, oxygen isotopes offer insight into body water at different life stages. δ¹⁸O values are used to understand drinking behaviors,{{Cite journal |last1=Feranec |first1=R. |last2=García |first2=N. |last3=DÍez |first3=J.C. |last4=Arsuaga |first4=J.L. |date=November 2010 |title=Understanding the ecology of mammalian carnivorans and herbivores from Valdegoba cave (Burgos, northern Spain) through stable isotope analysis |url=http://dx.doi.org/10.1016/j.palaeo.2010.08.006 |journal=Palaeogeography, Palaeoclimatology, Palaeoecology |volume=297 |issue=2 |pages=263–272 |doi=10.1016/j.palaeo.2010.08.006 |bibcode=2010PPP...297..263F |issn=0031-0182|url-access=subscription }} animal husbandry,{{Cite journal |last1=Vaiglova |first1=Petra |last2=Halstead |first2=Paul |last3=Pappa |first3=Maria |last4=Triantaphyllou |first4=Sevi |last5=Valamoti |first5=Soultana M. |last6=Evans |first6=Jane |last7=Fraser |first7=Rebecca |last8=Karkanas |first8=Panagiotis |last9=Kay |first9=Andrea |last10=Lee-Thorp |first10=Julia |author-link10=Julia Lee-Thorp |last11=Bogaard |first11=Amy |date=2018-06-07 |title=Of cattle and feasts: Multi-isotope investigation of animal husbandry and communal feasting at Neolithic Makriyalos, northern Greece |journal=PLOS ONE |language=en |volume=13 |issue=6 |pages=e0194474 |bibcode=2018PLoSO..1394474V |doi=10.1371/journal.pone.0194474 |issn=1932-6203 |pmc=5991682 |pmid=29879125 |doi-access=free}} and track mobility.{{Cite journal |last1=Sharpe |first1=Ashley E. |last2=Smith-Guzmán |first2=Nicole |last3=Curtis |first3=Jason |last4=Isaza-Aizpurúa |first4=Ilean |last5=Kamenov |first5=George D. |last6=Wake |first6=Thomas A. |last7=Cooke |first7=Richard G. |date=2021-04-01 |title=A preliminary multi-isotope assessment of human mobility and diet in pre-Columbian Panama |journal=Journal of Archaeological Science: Reports |language=en |volume=36 |article-number=102876 |doi=10.1016/j.jasrep.2021.102876 |s2cid=233527225 |issn=2352-409X|doi-access=free |bibcode=2021JArSR..36j2876S }} 97 burials from the ancient Maya citadel of Tikal were studied using oxygen isotopes.{{Cite journal |last=Wright |first=Lori E. |date=2012-09-01 |title=Immigration to Tikal, Guatemala: Evidence from stable strontium and oxygen isotopes |url=https://www.sciencedirect.com/science/article/pii/S027841651200013X |journal=Journal of Anthropological Archaeology |language=en |volume=31 |issue=3 |pages=334–352 |doi=10.1016/j.jaa.2012.02.001 |issn=0278-4165|url-access=subscription }} Results from tooth enamel identified statistically different individuals, interpreted to be individuals from Maya lowlands, Guatemala, and potentially Mexico. Historical context combined with isotopic data from burials were used to argue that migrant individuals were a part of lower and higher social classes within Tikal. Female migrants who arrived in Tikal during Early Classic period could have been the brides of Maya elite.

The sulfur stable isotope system is based on small, mass-dependent fractionations of sulfur isotopes. These fractionations are reported relative to Canyon Diablo Troilite (V-CDT), the agreed upon standard. The ratio of the most abundant sulfur isotope, ³²S, compared to rarer isotopes such as, ³³S, ³⁴S, and ³⁶S, is used to characterize biological signatures and geological reservoirs. The fractionation of ³⁴S (δ³⁴S) is particularly useful since it is the most abundant of the rare isotopes. This system is less commonly used on its own and typically complements studies of carbon and nitrogen.{{Cite journal |last1=Privat |first1=Karen L. |last2=O'Connell |first2=Tamsin C. |last3=Hedges |first3=Robert E. M. |date=2007-08-01 |title=The distinction between freshwater- and terrestrial-based diets: methodological concerns and archaeological applications of sulphur stable isotope analysis |url=https://www.sciencedirect.com/science/article/pii/S0305440306002147 |journal=Journal of Archaeological Science |language=en |volume=34 |issue=8 |pages=1197–1204 |doi=10.1016/j.jas.2006.10.008 |bibcode=2007JArSc..34.1197P |issn=0305-4403|hdl=1959.4/45662 |hdl-access=free }}{{Cite journal |last1=Buchardt |first1=Bjørn |last2=Bunch |first2=Vibeke |last3=Helin |first3=Pekka |date=2007-09-30 |title=Fingernails and diet: Stable isotope signatures of a marine hunting community from modern Uummannaq, North Greenland |url=https://www.sciencedirect.com/science/article/pii/S0009254107002847 |journal=Chemical Geology |language=en |volume=244 |issue=1 |pages=316–329 |doi=10.1016/j.chemgeo.2007.06.022 |bibcode=2007ChGeo.244..316B |issn=0009-2541|url-access=subscription }} In bioarchaeology, the sulfur system has been used to investigate paleodiets and spatial behaviors through the analysis of hair and bone collagen.{{Cite journal |last=Nehlich |first=Olaf |date=2015-03-01 |title=The application of sulphur isotope analyses in archaeological research: A review |url=https://www.sciencedirect.com/science/article/pii/S0012825214002220 |journal=Earth-Science Reviews |language=en |volume=142 |pages=1–17 |doi=10.1016/j.earscirev.2014.12.002 |bibcode=2015ESRv..142....1N |issn=0012-8252|url-access=subscription }} Dietary proteins incorporated into living organisms tend to determine the stable isotope values of their organic tissues. Methionine and cysteine are the canonical sulfur-containing amino acids. Of the two, δ³⁴S values of methionine are considered to better reflect isotopic compositions of dietary sulfur, since cysteine values are impacted by diet and internal cycling. While other stable isotope systems have significant trophic shifts, sulfur shows only a small shift (~0.5‰).

File:Sulfursignature JMueller illustrationV3.jpg{{Clear}}

Consumers yield isotopic signatures that reflect the sulfur reservoir(s) of the dietary protein source. Animal proteins sourced from marine ecosystems tend to have δ³⁴S values between +16 and +17‰,{{Cite journal |last1=Hoekstra |first1=P F |last2=Dehn |first2=L A |last3=George |first3=J C |last4=Solomon |first4=K R |last5=Muir |first5=D CG |last6=O'Hara |first6=T M |date=2002-02-01 |title=Trophic ecology of bowhead whales (Balaena mysticetus) compared with that of other arctic marine biota as interpreted from carbon-, nitrogen-, and sulfur-isotope signatures |url=http://dx.doi.org/10.1139/z01-229 |journal=Canadian Journal of Zoology |volume=80 |issue=2 |pages=223–231 |doi=10.1139/z01-229 |bibcode=2002CaJZ...80..223H |issn=0008-4301|url-access=subscription }} terrestrial plants range from -7‰ to +8‰, while proteins from freshwater and terrestrial ecosystems are highly variable. The sulfate content of the modern ocean is well-mixed with a δ³⁴S of approximately +21‰,{{Cite journal |last1=Paris |first1=Guillaume |last2=Sessions |first2=Alex L. |last3=Subhas |first3=Adam V. |last4=Adkins |first4=Jess F. |date=2013-05-08 |title=MC-ICP-MS measurement of δ34S and ∆33S in small amounts of dissolved sulfate |url=https://www.sciencedirect.com/science/article/pii/S0009254113000818 |journal=Chemical Geology |language=en |volume=345 |pages=50–61 |doi=10.1016/j.chemgeo.2013.02.022 |bibcode=2013ChGeo.345...50P |issn=0009-2541|url-access=subscription }} while riverine water is heavily influenced by sulfur-bearing minerals in surrounding bedrock and terrestrial plants are influenced by the sulfur content of local soils. Estuarian ecosystems have increased complexity due to seawater and river inputs. The extreme range of δ³⁴S values for freshwater ecosystems often interferes with terrestrial signals, making it difficult to use the sulfur system as the sole tool in paleodiet studies.

Various studies have analyzed the isotopic ratios of sulfur in mummified hair.{{Cite journal |last1=Aufderheide |first1=Arthur C. |last2=Muñoz |first2=Ivan |last3=Arriaza |first3=Bernardo |date=June 1993 |title=Seven Chinchorro mummies and the prehistory of northern Chile |url=https://onlinelibrary.wiley.com/doi/10.1002/ajpa.1330910205 |journal=American Journal of Physical Anthropology |language=en |volume=91 |issue=2 |pages=189–201 |doi=10.1002/ajpa.1330910205 |pmid=8317560 |issn=0002-9483|url-access=subscription }}{{Cite journal |last1=Jones |first1=M. K. |last2=Briggs |first2=D. E. G. |last3=Eglington |first3=G. |last4=Hagelberg |first4=E. |last5=Macko |first5=Stephen A. |last6=Engel |first6=Michael H. |last7=Andrusevich |first7=Vladimir |last8=Lubec |first8=Gert |last9=O'Connell |first9=Tamsin C. |last10=Hedges |first10=Robert E. M. |date=1999-01-29 |title=Documenting the diet in ancient human populations through stable isotope analysis of hair |journal=Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences |volume=354 |issue=1379 |pages=65–76 |doi=10.1098/rstb.1999.0360 |pmc=1692445 |pmid=10091248}}{{Cite journal |last1=Touzeau |first1=Alexandra |last2=Amiot |first2=Romain |last3=Blichert-Toft |first3=Janne |last4=Flandrois |first4=Jean-Pierre |last5=Fourel |first5=François |last6=Grossi |first6=Vincent |last7=Martineau |first7=François |last8=Richardin |first8=Pascale |last9=Lécuyer |first9=Christophe |date=2014-06-01 |title=Diet of ancient Egyptians inferred from stable isotope systematics |url=https://www.sciencedirect.com/science/article/pii/S0305440314000843 |journal=Journal of Archaeological Science |language=en |volume=46 |pages=114–124 |doi=10.1016/j.jas.2014.03.005 |bibcode=2014JArSc..46..114T |issn=0305-4403|url-access=subscription }} Hair is a good candidate for sulfur studies as it typically contains at least 5% elemental sulfur. One study incorporated sulfur isotope ratios into their paleodietary investigation of four mummified child victims of Incan sacrificial practices.{{Cite journal |last1=Wilson |first1=Andrew S. |last2=Taylor |first2=Timothy |last3=Ceruti |first3=Maria Constanza |last4=Chavez |first4=Jose Antonio |last5=Reinhard |first5=Johan |last6=Grimes |first6=Vaughan |last7=Meier-Augenstein |first7=Wolfram |last8=Cartmell |first8=Larry |last9=Stern |first9=Ben |last10=Richards |first10=Michael P. |last11=Worobey |first11=Michael |date=2007-10-16 |title=Stable isotope and DNA evidence for ritual sequences in Inca child sacrifice |journal=Proceedings of the National Academy of Sciences |language=en |volume=104 |issue=42 |pages=16456–16461 |doi=10.1073/pnas.0704276104 |issn=0027-8424 |pmc=2034262 |pmid=17923675|bibcode=2007PNAS..10416456W |doi-access=free }} δ³⁴S values helped them conclude that the children had not been eating marine protein before their death. Historical insight coupled with consistent sulfur signatures for three of the children suggests that they were living in the same location 6 months prior to the sacrifice. Studies have measured δ³⁴S values of bone collagen, though the interpretation of these values was not reliable until quality criteria were published in 2009.{{Cite journal |last1=Nehlich |first1=Olaf |last2=Richards |first2=Michael P. |date=2009-03-01 |title=Establishing collagen quality criteria for sulphur isotope analysis of archaeological bone collagen |journal=Archaeological and Anthropological Sciences |language=en |volume=1 |issue=1 |pages=59–75 |doi=10.1007/s12520-009-0003-6 |s2cid=128784144 |issn=1866-9565|doi-access=free |bibcode=2009ArAnS...1...59N }} Though bone collagen is abundant in skeletal remains, less than 1% of the tissue is made of sulfur, making it imperative that these studies carefully assess the meaning of bone collagen δ³⁴S values.

DNA analysis of past populations is used to genetically determine sex, determine genetic relatedness, understand marriage patterns, and investigate prehistoric migration.{{cite journal|last1=Kaestle|first1=Fredericka A.|last2=Horsburgh|first2=K. Ann|year=2002|title=Ancient DNA in Anthropology|journal=Yearbook of Physical Anthropology|volume=45|pages=92–130|doi=10.1002/ajpa.10179|pmid=12653310|doi-access=free}}

In 2012 archaeologists found skeletal remains of an adult male. He was buried under a car park in England. DNA evidence allowed the archaeologists to confirm that the remains belonged to Richard III, the former king of England who died in the Battle of Bosworth.{{Cite web|last=Valk|first=Diana|date=2015-05-06|title=How Forensic Techniques Aid Archaeology|url=https://daily.jstor.org/forensic-archaeology/|access-date=2020-12-05|website=JSTOR Daily|language=en-US}}

In 2021, Canadian researchers analyzed skeletal remains found on King William Island, identifying them as belonging to Warrant Officer John Gregory, an engineer serving aboard HMS Erebus in the ill-fated 1845 Franklin Expedition. He was the first expedition member to be identified by DNA analysis.{{Cite journal|last1=Stenton|first1=Douglas R.|last2=Fratpietro|first2=Stephen|last3=Keenleyside|first3=Anne|last4=Park|first4=Robert W.|date=2021|title=DNA identification of a sailor from the 1845 Franklin northwest passage expedition|url=http://dx.doi.org/10.1017/s0032247421000061|journal=Polar Record|volume=57|article-number=e14 |doi=10.1017/s0032247421000061|bibcode=2021PoRec..57E..14S |s2cid=233412371|issn=0032-2474|url-access=subscription}}

The study of human remains can illuminate the relationship between physical bodies and socio-cultural conditions and practices, via a biocultural bioarchaeology model.{{Citation|last1=Turner|first1=Bethany L.|title=Biocultural perspectives in bioarchaeology|date=2016|url=https://onlinelibrary.wiley.com/doi/abs/10.1002/9781118962954.ch21|work=New Directions in Biocultural Anthropology|pages=427–451|publisher=John Wiley & Sons, Ltd|language=en|doi=10.1002/9781118962954.ch21|isbn=978-1-118-96295-4|access-date=2020-12-05|last2=Klaus|first2=Haagen D.|url-access=subscription}} Bioarchaeology is typically regarded as a positivist, science-based discipline, while the social sciences are regarded as constructivist. Bioarchaeology has been criticized for having little to no concern for culture or history. One scholar argued that scientific/forensic scholarship ignores cultural/historic factors. He proposed that a biocultural version of bioarchaeology offered a more meaningful, nuanced, and relevant picture, especially for descent populations.{{cite journal |last1=Blakey |first1=Michael L |year=2001 |title=Bioarchaeology of the African Diaspora in the Americas: Its Origins and Scope |journal=Annual Review of Anthropology |volume=30 |pages=387–422 |doi=10.1146/annurev.anthro.30.1.387}}Blakey, Michael L. Introduction: Section 1: Background of the New York African Burial Ground Project. Philadelphia, PA: US Department of the Interior, National Park Service, 2004.

Biocultural bioarchaeology combines standard forensic techniques with investigations of demography and epidemiology in order to assess socioeconomic conditions experienced by human communities. For example, incorporation of analysis of grave goods can further the understanding of daily activities.

Some bioarchaeologists view the discipline as a crucial interface between the science and the humanities; as the human body is made and re-made by both biological and cultural factors.Lorentz, Kirsi. "From Bodies to Bones and Back: Theory and Human Bioarchaeology." Between Biology and Culture. Ed. Holger Schutkowski. Cambridge Studies in Biological and Evolutionary Anthropology: Cambridge University Press, 2008. 273–303.

Another type of bioarchaeology focuses on quality of life, lifestyle, behavior, biological relatedness, and population history.{{Cite book |last=Buikstra |first=Jane E |url=http://dx.doi.org/10.4324/9781315432939 |title=Bioarchaeology: The Contextual Analysis of Human Remains |date=2017-03-02 |publisher=Routledge |isbn=978-1-315-43293-9 |editor-last=Buikstra |editor-first=Jane |pages=347–258 |chapter=Introduction to Section III: On to the 21st Century |doi=10.4324/9781315432939 |editor-last2=Beck |editor-first2=Lane A}}Larsen, Clark Spencer. "The Changing Face of Bioarchaeology: An Interdisciplinary Science." Bioarchaeology: The Contextual Analysis of Human Remains. Eds. Jane E. Buikstra and Lane A Beck: Academic Press Elsevier, 2006. 359–74.Larsen, Clark Spencer. Skeletons in Our Closet: Revealing Our Past through Bioarchaeology. Princeton and Oxford: Princeton University Press, 2000. It does not closely link skeletal remains to their archaeological context, and may best be viewed as a "skeletal biology of the past".Buikstra, Jane E. "Introduction to Section III: On to the 21st Century." p. 354. Bioarchaeology: The Contextual Analysis of Human Remains. Eds. Jane E. Buikstra and Lane A Beck: Academic Press/Elsevier, 2006. 347–258.

Inequalities exist in all human societies.{{cite journal|last1=Osborne|first1=Robin|year=2007|title=Is Archaeology Equal to Equality|journal=World Archaeology|volume=39|issue=2|pages=143–50|doi=10.1080/00438240701249447|s2cid=144248493}} Bioarchaeology has helped to dispel the idea that life for foragers of the past was "nasty, brutish and short"; bioarchaeological studies reported that foragers of the past were often healthy, while agricultural societies tended to have increased incidence of malnutrition and disease.{{cite journal|last1=Lambert|first1=Patricia M|year=2009|title=Health Versus Fitness: Competing Themes in the Origins and Spread of Agriculture?|journal=Current Anthropology|volume=50|issue=5|pages=603–08|doi=10.1086/605354|pmid=20642145|s2cid=23599320}} One study compared foragers from Oakhurst to agriculturalists from K2 and Mapungubwe and reported that agriculturalists from K2 and Mapungubwe were not subject to the lower nutritional levels expected.{{cite journal |last1=Steyne |first1=Maryna |year=1997 |title=A Reassessment of the Human Skeletons from K2 and Mapungubwe (South Africa) |journal=The South African Archaeological Bulletin |volume=52 |issue=165 |pages=14–20 |doi=10.2307/3888972 |jstor=3888972}}

Danforth argues that more "complex" state-level societies display greater health differences between elites and the rest of society, with elites having the advantage, and that this disparity increases as societies become more unequal. Some status differences in society do not necessarily mean radically different nutritional levels; Powell did not find evidence of great nutritional differences between elites and commoners, but did find lower rates of anemia among elites in Moundville.{{Cite journal |last=Powell |first=Mary Lucas |date=September 1989 |editor-last=Turner |editor-first=Kenneth R. |title=Biological Anthropology: Status and Health in Prehistory: A Case Study of the Moundville Chiefdom. |url=http://dx.doi.org/10.1525/aa.1989.91.3.02a00510 |journal=American Anthropologist |volume=91 |issue=3 |pages=794–795 |doi=10.1525/aa.1989.91.3.02a00510 |issn=0002-7294|url-access=subscription }}

An area of increasing interest interested in understanding inequality is the study of violence.{{Cite book |last1=Martin |first1=Debra L. |url={{google books|plainurl=y|id=o33SEAAAQBAJ}} |title=The Bioarchaeology of Violence |last2=Harrod |first2=Ryan P. |date=2012-08-05 |publisher=University Press of Florida |isbn=978-0-8130-4363-0 |language=en}} Researchers analyzing traumatic injuries on human remains have shown that social status and gender can have a significant impact on exposure to violence.{{cite journal|last1=Schug|first1=Robbins|last2=Gwen|last3=Gray|first3=Kelsey|last4=Mushrif-Tripathy|first4=Veena|last5=Ram Sankhyan|first5=Anek|year=2012|title=A Peaceful Realm? Trauma and social differentiation at Harappa|journal=International Journal of Paleopathology|volume=2|issue=2–3|pages=136–147|doi=10.1016/j.ijpp.2012.09.012|pmid=29539378|bibcode=2012IJPal...2..136R |s2cid=3933522 |url=http://libres.uncg.edu/ir/uncg/f/G_Robbins_Schug_Peaceful_2012.pdf}}Martin, Debra L., Ryan P. Harrod, and Misty Fields. "Beaten Down and Worked to the Bone: Bioarchaeological Investigations of Women and Violence in the Ancient Southwest." Landscapes of Violence 1.1 (2010): Article 3. http://scholarworks.umass.edu/lov/vol1/iss1/3/{{cite journal|last1=Wilkinson|first1=R G.|last2=Wagenen|first2=K M. Van|year=1993|title=Violence against Women: Prehistoric Skeletal Evidence from Michigan|journal=Midcontinental Journal of Archaeology|volume=18|pages=190–216}} Numerous researchers study violence in human remains, exploring violent behavior, including intimate partner violence,Walker, Phillip L. "Wife Beating, Boxing, and Broken Noses: Skeletal Evidence for the Cultural Patterning of Violence." Troubled Times: Violence and Warfare in the Past. Eds. Martin, Debra L. and David W. Frayer. Amsterdam: Gordon and Breach, 1997. 145–80. child abuse,{{cite journal|last1=Walker|first1=Phillip L.|last2=Collins Cook|first2=Della|last3=Lambert|first3=Patricia M.|year=1997|title=Skeletal Evidence for Child Abuse: A Physical Anthropological Perspective|journal=Journal of Forensic Sciences|volume=42|issue=2|pages=196–207|pmid=9068177|doi=10.1520/JFS14098J}} institutional abuse,{{cite journal|last1=De la Cova|first1=Carlina|year=2010|title=Cultural Patterns of Trauma among 19th-Century-Born Males in Cadaver Collections|journal=American Anthropologist|volume=112|issue=4|pages=589–606|doi=10.1111/j.1548-1433.2010.01278.x|pmid=21132946}} torture,{{cite journal|last1=Osterholtz|first1=Anna J|year=2012|title=The Social Role of Hobbling and Torture: Violence in the Prehistoric Southwest|journal=International Journal of Paleopathology|volume=2|issue=2–3|pages=148–155|doi=10.1016/j.ijpp.2012.09.011|pmid=29539379|bibcode=2012IJPal...2..148O }}{{cite journal|last1=Blondiaux|first1=Joel|display-authors=etal|year=2012|title=Bilateral Fractures of the Scapula: Possible Archeological Examples of Beatings from Europe, Africa and America|journal=International Journal of Paleopathology|volume=2|issue=4|pages=223–230|doi=10.1016/j.ijpp.2012.10.002|pmid=29539369|bibcode=2012IJPal...2..223B }} warfare,Milner, George R. "An Osteological Perspective on Prehistoric Warfare." Regional Approaches to Mortuary Analysis. Ed. Beck, Lane A. New York: Plenum Press, 1995. 221–44.{{cite journal|last1=Andrushko|first1=Valerie A.|last2=Schwitalla|first2=Al W.|last3=Walker|first3=Phillip L.|year=2010|title=Trophy-Taking and Dismemberment as Warfare Strategies in Prehistoric Central California|journal=American Journal of Physical Anthropology|volume=141|issue=1|pages=83–96|doi=10.1002/ajpa.21117|pmid=19544576}} human sacrifice,Tiesler, Vera, and Andrea Cucina, eds. New Perspectives on Human Sacrifice and Ritual Body Treatments in Ancient Maya Society. New York: Springer, 2007.{{cite journal|last1=Tung|first1=Tiffiny A.|last2=Knudson|first2=Kelly J.|year=2010|title=Childhood Lost: Abductions, Sacrifice, and Trophy Heads of Children in the Wari Empire of the Ancient Andes|journal=Latin American Antiquity|volume=21|issue=1|pages=44–66|doi=10.7183/1045-6635.21.1.44|s2cid=163558123}} and structural violence.Pérez, Ventura R. "From the Singing Tree to the Hanging Tree: Structural Violence and Death with the Yaqui Landscape." Landscapes of Violence 1.1 (2010): Article 4. http://scholarworks.umass.edu/lov/vol1/iss1/9/Klaus, Haagen D. "The Bioarchaeology of Structural Violence: A Theoretical Model and a Case Study." 2012. The Bioarchaeology of Violence. Eds. Martin, Debra L., Ryan P. Harrod and Ventura R. Pérez. Gainesville: University of Florida Press. 29–62.

Ethical issues with bioarchaeology revolve around the treatment and respect for the dead.{{Cite journal |date=2019 |editor-last=Squires |editor-first=Kirsty |editor2-last=Errickson |editor2-first=David |editor3-last=Márquez-Grant |editor3-first=Nicholas |title=Ethical Approaches to Human Remains |url=https://link.springer.com/book/10.1007/978-3-030-32926-6 |journal=SpringerLink |language=en |doi=10.1007/978-3-030-32926-6 |isbn=978-3-030-32925-9 }} Large-scale skeletal collections were first amassed in the US in the 19th century, largely the remains of Native Americans. No permission was granted by surviving family for study and display. Federal laws such as 1990's NAGPRA (Native American Graves Protection and Repatriation Act) allowed Native Americans to regain control over their ancestors' remains and associated artifacts.

Many archaeologists did not realize that many people perceive archaeologists as non-productive and/or grave robbers.{{cite journal|last1=Patterson|first1=Thomas C|year=1999|title=The Political Economy of Archaeology in the United States|journal=Annual Review of Anthropology|volume=28|pages=155–75|doi=10.1146/annurev.anthro.28.1.155}} Concerns about mistreatment of remains are not unfounded: in a 1971 Minnesota excavation, White and Native American remains were treated differently; Whites were reburied, while Native Americans were moved to a natural history museum. African American bioarchaeology grew after NAGPRA and its effect of ending the study of Native American remains.

Bioarchaeology in Europe was not as disrupted by repatriation issues. However, because much of European archaeology has been focused on classical roots, artifacts and art have been emphasized and Roman and post-Roman skeletal remains were nearly completely neglected until the 1980s. In prehistoric European archaeology, biological remains began to be analyzed earlier than in classical archaeology.

While ethical approaches to the excavation and analysis of physical human remains have received considerable attention, professional and academic dialogue regarding how to appropriately record, share, and display human remains in the digital realm is less developed. While digital technologies for recording and analysing human remains are increasingly accessible, justification for such recording and analysis is essential e.g. 3D scanning performed simply because it is possible is inappropriate and disrespectful to the deceased.{{Citation |last1=Smith |first1=Sian E. |title=3D Data in Human Remains Disciplines: The Ethical Challenges |date=2019 |work=Ethical Approaches to Human Remains: A Global Challenge in Bioarchaeology and Forensic Anthropology |pages=315–346 |editor-last=Squires |editor-first=Kirsty |url=https://doi.org/10.1007/978-3-030-32926-6_14 |access-date=2025-07-08 |place=Cham |publisher=Springer International Publishing |language=en |doi=10.1007/978-3-030-32926-6_14 |isbn=978-3-030-32926-6 |last2=Hirst |first2=Cara S. |editor2-last=Errickson |editor2-first=David |editor3-last=Márquez-Grant |editor3-first=Nicholas}}{{cite journal|last1=Bohling|first1=Solange|title=Online dissemination of 3D bioarchaeological data: An exploration of ethics, user preferences, and contextualisation in an official digital repository setting|journal=Internet Archaeology|date=2025|issue=69|doi=10.11141/ia.69.9|doi-access=free|url=https://intarch.ac.uk/journal/issue69/9/index.html}}

• Ancient DNA
• Biocultural anthropology
• Odontometrics
• Osteoarchaeology
• Paleopathology
• Zooarchaeology

• J. Buikstra, 1977. "Biocultural dimensions of archaeological study: a regional perspective". In:Biocultural adaptation in prehistoric America, pp. 67–84. University of Georgia Press.
• J. Buikstra and L. Beck, eds., 2006. "Bioarchaeology: the Contextual Study of Human Remains." Elsevier.
• M. Katzenberg and S. Saunders, eds., 2000. Biological anthropology of the human skeleton. Wiley.
• K. Killgrove, 2014. [https://www.academia.edu/3250303/Bioarchaeology_Oxford_Annotated_Bibliography_ Bioarchaeology] {{Webarchive|url=https://web.archive.org/web/20190626112001/https://www.academia.edu/3250303/Bioarchaeology_Oxford_Annotated_Bibliography_ |date=2019-06-26 }}. In: Oxford Annotated Bibliographies Online. Oxford.
• C.S. Larsen, 1997. Bioarchaeology: interpreting behavior from the human skeleton. Cambridge University Press.
• {{cite journal|doi=10.11141/ia.53.6 | issue=53 | title=Beyond Extractive Practice: Bioarchaeology, Geoarchaeology and Human Palaeoecology for the People | year=2019 | journal=Internet Archaeology | last1 = Law | first1 = Matt |doi-access=free}}
• S. Mays, 1998. The archaeology of human bones. Routledge.
• Samuel J. Redman, 2016. Bone Rooms: From Scientific Racism to Human Prehistory in Museums. Harvard University Press.
• M. Parker Pearson, 2001. The archaeology of death and burial. Texas A&M University Press.
• D. Ubelaker, 1989. Human skeletal remains: excavation, analysis, interpretation. Taraxacum.
• T. White, 1991. Human osteology. Academic Press.

Organizations

• [http://physanth.org/ American Association of Physical Anthropologists]
• [https://web.archive.org/web/20131214043959/http://www.aaanet.org/sections/bas/index.html Biological Anthropology Section of the American Anthropological Association]
• [http://www.babao.org.uk/ British Association of Biological Anthropologists and Osteoarchaeologists]
• [http://capa.fenali.net/ Canadian Association for Physical Anthropology]

Journals

• [http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1096-8644/ American Journal of Physical Anthropology]
• [http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1099-1212/ International Journal of Osteoarchaeology]
• [http://www.sciencedirect.com/science/journal/0018442X/ HOMO: Journal of Comparative Human Biology]
• [http://www.journals.elsevier.com/international-journal-of-paleopathology/ International Journal of Paleopathology]
• [http://www.anthropology.uw.edu.pl Bioarchaeology of the Near East]

Other

• [http://plaza.ufl.edu/maurih00/paleopathology.html Paleopathology]
• [https://web.archive.org/web/20101114084318/http://www.africanburialground.gov/abg_main.htm The African Burial Ground]
• [https://web.archive.org/web/20100610011847/http://shesc.asu.edu/bioarchaeology Bioarchaeology and the Center for Bioarchaeological Research]
• [https://www.nps.gov/subjects/nagpra/index.htm National NAGPRA homepage]
• [http://www.bonesdontlie.com Bones Don't Lie Blog]
• [http://www.poweredbyosteons.org Powered by Osteons Blog]
• [https://www.forbes.com/sites/kristinakillgrove Kristina Killgrove's Bioarchaeology Blog at Forbes]

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Category:1970s neologisms

Category:Collections care

Category:Conservation and restoration of cultural heritage

Category:Museology

Category:Cultural heritage

Category:Archaeological science

Category:Art and cultural repatriation

Category:Zooarchaeology

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