CORO6
{{Short description|Protein-coding gene in the species Homo sapiens}}
{{Infobox gene}}
Coronin-6 also known as coronin-like protein E (Clipin-E) is a protein that in humans is encoded by the CORO6 gene.
Coronin-6 belongs to the coronin family which is an actin binding protein.{{cite journal |vauthors=de Hostos EL |title=The coronin family of actin-associated proteins |journal=Trends in Cell Biology |volume=9 |issue=9 |pages=345–350 |date=September 1999 |pmid=10461187 |doi=10.1016/S0962-8924(99)01620-7}}{{cite web|title=Cronin-6 Homo Sapiens|url=https://www.ncbi.nlm.nih.gov/protein/NP_116243.2|publisher=NCBI|access-date=28 April 2013}} Human CORO6 gene is located on chromosome 17 on the cytogenetic band 17 p11.2.{{cite web|title=CORO6|url=https://www.genecards.org/cgi-bin/carddisp.pl?gene=CORO6&search=coro6|publisher=GeneCards|access-date=28 April 2013}} Gene CORO6 is well conserved across domain of eukaryote organisms from fungus to animal.{{cite web|title=CORO6|url=https://www.ncbi.nlm.nih.gov/homologene?Db=homologene&Cmd=Retrieve&list_uids=104099|publisher=HomoloGene|access-date=28 April 2013}}
Expression
=EST profile=
Based on the EST profile, CORO6 expressed in high level at the larynx, nerve and muscle. CORO6 has also been shown to be expressed in high levels in the breast (mammary gland) tumor. During the human development stage, the higher level of CORO6 expressed at blastocyst and adult.{{cite web|title=CORO6 expression level.|url=https://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.143046|publisher=EST profile|access-date=28 April 2013}}
= Transcript Variant =
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron. Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript . CORO6 contains 21 distinct gt-ag introns. Transcription produces 10 alternatively spliced mRNA. There are 3 probable alternative promoters, and validated alternative polyadenylation sites.{{cite web|title=CORO6|url=https://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?db=human&term=coro6&submit=Go|publisher=ACEview}}
Structure
CORO6 protein sequence contains WD-40 repeats. WD40 domain is a structural motif found in Eukaryotes and cover variety of functions, such as adaptor or regulatory modules in signal transduction, pre-mRNA processing and cytoskeletal assembly. It usually terminating at WD dipeptide at its C-terminus and is about 40 residues long, so called WD40.{{cite web|title=WD-40 superfamily|url=https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cl02567|publisher=conserved domain|access-date=28 April 2013}}
The structure of CORO6 is predicted by using Phyre2 program. It is similar to the crystal structure of murine coronin-1. 390 residues ( 83% of CORO6 protein sequence) have been modelled with 100.0% confidence by the single highest scoring template. Image coloured by rainbow N → C terminus
Homology
= Paralogs =
Human proteins which are the paralogs to CORO6,
The table compared Homo sapiens protein CORO6 to its paralogs
| class="wikitable" | ||||||
| Name of paralogs | CORO6 | CORO1A | CORO1B | CORO2A | CORO2B | CORO7 |
|---|---|---|---|---|---|---|
| Accession number | NP_116243 | NP_009005 | NP_065174 | NP_438171 | NP_006082 | NP_078811 |
| Sequence length | 472 aa | 461 aa | 489 aa | 525 aa | 480 aa | 925 aa |
| Sequence identity | 67% | 67% | 45% | 45% | 32% | |
| Sequence similarity | 81% | 80% | 64% | 63% | 49% |
By comparing its paralogs we found that CORO1A and CORO1B are most related to CORO6.
= Orthologs =
CORO6 is highly conserved throughout the organisms from vertebrate to fungus, the organisms listed in the table are some representatives.
| class="wikitable" | |||||||
| Genus and species (Orthologs comparison) | Homo sapiens | Pan troglodytes | Canis familiaris | Anolis carolinensis | Danio rerio | Saccharomyces cerevisiae | Plasmodium falciparum |
|---|---|---|---|---|---|---|---|
| Common name | Human | Chimpanzee | Dog | Lizard | Zebrafish | Baker's Yeast | Malaria parasite |
| Date of divergence from human lineage | 6.3 MYA | 94.2 MYA | 269 MYA | 400.1 MYA | 1215.8 MYA | 1381.2 MYA | |
| Accession number | NP_116243 | XP_001137660 | XP_548302 | XP_0003227217 | NP_956690 | NP_013533 | XP_001350896 |
| Sequence length | 472 aa | 471 aa | 472 aa | 471 aa | 436 aa | 651 aa | 602 aa |
| Sequence identity to human | 96% | 98% | 83% | 78% | 42% | 31% | |
| Sequence similarity to human | 97% | 99% | 90% | 90% | 62% | 52% |
Clinical significance
There are several clinical studies about that have been performed by using microarray indicating that CORO6 is positively related to allergic nasal epithelium response to house dust mite allergen in vitro.{{cite web|title=Allergic nasal epithelium response to house dust mite allergen in vitro.pnj|url=https://www.ncbi.nlm.nih.gov/geo/tools/profileGraph.cgi?ID=GDS3082:1552301_a_at|publisher=GEO profile|access-date=9 May 2013}}
References
{{Reflist}}
External links
- {{UCSC gene info|CORO6}}