Login
Login

EEF2

{{Short description|Protein-coding gene in the species Homo sapiens}}

{{Infobox_gene}}

Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. It is the archaeal and eukaryotic counterpart of bacterial EF-G.{{cite journal | vauthors = Rapp G, Klaudiny J, Hagendorff G, Luck MR, Scheit KH | title = Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells | journal = Biological Chemistry Hoppe-Seyler | volume = 370 | issue = 10 | pages = 1071–5 | date = October 1989 | pmid = 2610926 | doi = 10.1515/bchm3.1989.370.2.1071 }}{{cite journal | vauthors = Kaneda Y, Yoshida MC, Kohno K, Uchida T, Okada Y | title = Chromosomal assignment of the gene for human elongation factor 2 | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 81 | issue = 10 | pages = 3158–62 | date = May 1984 | pmid = 6427766 | pmc = 345240 | doi = 10.1073/pnas.81.10.3158 | bibcode = 1984PNAS...81.3158K | doi-access = free }}{{cite web | title = Entrez Gene: EEF2 eukaryotic translation elongation factor 2| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1938}}{{cite journal | vauthors = Narrowe AB, Spang A, Stairs CW, Caceres EF, Baker BJ, Miller CS, Ettema TJ | title = Complex Evolutionary History of Translation Elongation Factor 2 and Diphthamide Biosynthesis in Archaea and Parabasalids | journal = Genome Biology and Evolution | volume = 10 | issue = 9 | pages = 2380–2393 | date = September 2018 | pmid = 30060184 | pmc = 6143161 | doi = 10.1093/gbe/evy154 | doi-access = free }}

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the ribosome. This protein is completely inactivated by EF-2 kinase phosphorylation.

aEF2/eEF2 found in most archaea and eukaryotes, including humans, contains a post translationally modified histidine diphthamide. It is the target of diphtheria toxin (from Corynebacterium diphtheriae), and exotoxin A (from Pseudomonas aeruginosa).{{cite journal | vauthors = Jørgensen R, Merrill AR, Andersen GR | title = The life and death of translation elongation factor 2 | journal = Biochemical Society Transactions | volume = 34 | issue = Pt 1 | pages = 1–6 | date = February 2006 | pmid = 16246167 | doi = 10.1042/BST20060001 }} The inactivation of EF-2 by toxins inhibits protein production in the host, causing symptoms due to loss of function in affected cells.

References

{{reflist}}

Further reading

{{refbegin|35em}}

  • {{cite journal | vauthors = Hanes J, Freudenstein J, Rapp G, Scheit KH | title = Construction of a plasmid containing the complete coding region of human elongation factor 2 | journal = Biological Chemistry Hoppe-Seyler | volume = 373 | issue = 4 | pages = 201–4 | date = April 1992 | pmid = 1596361 | doi = 10.1515/bchm3.1992.373.1.201 }}
  • {{cite journal | vauthors = Nygård O, Nilsson L | title = Kinetic determination of the effects of ADP-ribosylation on the interaction of eukaryotic elongation factor 2 with ribosomes | journal = The Journal of Biological Chemistry | volume = 265 | issue = 11 | pages = 6030–4 | date = April 1990 | doi = 10.1016/S0021-9258(19)39286-5 | pmid = 2318846 | doi-access = free }}
  • {{cite journal | vauthors = Rapp G, Mucha J, Einspanier R, Luck M, Scheit KH | title = Cloning and sequence analysis of a cDNA from human ovarian granulosa cells encoding the C-terminal part of human elongation factor 2 | journal = Biological Chemistry Hoppe-Seyler | volume = 369 | issue = 4 | pages = 247–50 | date = April 1988 | pmid = 2840927 | doi = 10.1515/bchm3.1988.369.1.247 }}
  • {{cite journal | vauthors = Kaneda Y, Hayes H, Uchida T, Yoshida MC, Okada Y | s2cid = 33919242 | title = Regional assignment of five genes on human chromosome 19 | journal = Chromosoma | volume = 95 | issue = 1 | pages = 8–12 | year = 1987 | pmid = 3034518 | doi = 10.1007/BF00293835 }}
  • {{cite journal | vauthors = Nairn AC, Palfrey HC | title = Identification of the major Mr 100,000 substrate for calmodulin-dependent protein kinase III in mammalian cells as elongation factor-2 | journal = The Journal of Biological Chemistry | volume = 262 | issue = 36 | pages = 17299–303 | date = December 1987 | doi = 10.1016/S0021-9258(18)45377-X | pmid = 3693353 | doi-access = free }}
  • {{cite journal | vauthors = Shestakova EA, Motuz LP, Minin AA, Gavrilova LP | title = Study of localization of the protein-synthesizing machinery along actin filament bundles | journal = Cell Biology International | volume = 17 | issue = 4 | pages = 409–16 | date = April 1993 | pmid = 8318952 | doi = 10.1006/cbir.1993.1079 | s2cid = 37430592 }}
  • {{cite journal | vauthors = Redpath NT, Price NT, Severinov KV, Proud CG | title = Regulation of elongation factor-2 by multisite phosphorylation | journal = European Journal of Biochemistry | volume = 213 | issue = 2 | pages = 689–99 | date = April 1993 | pmid = 8386634 | doi = 10.1111/j.1432-1033.1993.tb17809.x | doi-access = free }}
  • {{cite journal | vauthors = Knebel A, Haydon CE, Morrice N, Cohen P | title = Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and -insensitive pathways | journal = The Biochemical Journal | volume = 367 | issue = Pt 2 | pages = 525–32 | date = October 2002 | pmid = 12171600 | pmc = 1222910 | doi = 10.1042/BJ20020916 }}
  • {{cite journal | vauthors = Yin X, Fontoura BM, Morimoto T, Carroll RB | title = Cytoplasmic complex of p53 and eEF2 | journal = Journal of Cellular Physiology | volume = 196 | issue = 3 | pages = 474–82 | date = September 2003 | pmid = 12891704 | doi = 10.1002/jcp.10329 | s2cid = 25241366 }}
  • {{cite journal | vauthors = Ryazanova LV, Dorovkov MV, Ansari A, Ryazanov AG | title = Characterization of the protein kinase activity of TRPM7/ChaK1, a protein kinase fused to the transient receptor potential ion channel | journal = The Journal of Biological Chemistry | volume = 279 | issue = 5 | pages = 3708–16 | date = January 2004 | pmid = 14594813 | doi = 10.1074/jbc.M308820200 | doi-access = free }}
  • {{cite journal | vauthors = Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP | display-authors = 6 | title = Large-scale characterization of HeLa cell nuclear phosphoproteins | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 101 | issue = 33 | pages = 12130–5 | date = August 2004 | pmid = 15302935 | pmc = 514446 | doi = 10.1073/pnas.0404720101 | bibcode = 2004PNAS..10112130B | doi-access = free }}
  • {{cite journal | vauthors = Elo MA, Karjalainen HM, Sironen RK, Valmu L, Redpath NT, Browne GJ, Kalkkinen N, Helminen HJ, Lammi MJ | display-authors = 6 | title = High hydrostatic pressure inhibits the biosynthesis of eukaryotic elongation factor-2 | journal = Journal of Cellular Biochemistry | volume = 94 | issue = 3 | pages = 497–507 | date = February 2005 | pmid = 15534876 | doi = 10.1002/jcb.20333 | s2cid = 25000605 }}
  • {{cite journal | vauthors = Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M | s2cid = 4344740 | title = Nucleolar proteome dynamics | journal = Nature | volume = 433 | issue = 7021 | pages = 77–83 | date = January 2005 | pmid = 15635413 | doi = 10.1038/nature03207 | bibcode = 2005Natur.433...77A }}
  • {{cite journal | vauthors = Shibatani T, David LL, McCormack AL, Frueh K, Skach WR | title = Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits | journal = Biochemistry | volume = 44 | issue = 16 | pages = 5982–92 | date = April 2005 | pmid = 15835887 | doi = 10.1021/bi047328f }}
  • {{cite journal | vauthors = Ahmed M, Forsberg J, Bergsten P | title = Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry | journal = Journal of Proteome Research | volume = 4 | issue = 3 | pages = 931–40 | year = 2005 | pmid = 15952740 | doi = 10.1021/pr050024a }}
  • {{cite journal | vauthors = Gevaert K, Staes A, Van Damme J, De Groot S, Hugelier K, Demol H, Martens L, Goethals M, Vandekerckhove J | display-authors = 6 | title = Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC | journal = Proteomics | volume = 5 | issue = 14 | pages = 3589–99 | date = September 2005 | pmid = 16097034 | doi = 10.1002/pmic.200401217 | s2cid = 895879 }}

{{refend}}

External links

  • {{MeshName|Peptide+Elongation+Factor+2}}

{{GeneticTranslation}}

{{gene-19-stub}}