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List of restriction enzyme cutting sites: A#Whole list navigation

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{{Main|List of restriction enzyme cutting sites|Restriction enzyme}}

{{Use dmy dates|date=January 2020}}

{{LORECSListAminoAcids}}

This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site.

The following information is given for each enzyme:

  • Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature,{{cite journal | vauthors = Smith HO, Nathans D | title = Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes | journal = J. Mol. Biol. | volume = 81 | issue = 3 | pages = 419–23 | date=December 1973 | pmid = 4588280 | doi = 10.1016/0022-2836(73)90152-6}}{{cite journal | vauthors = Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY | title = A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes | journal = Nucleic Acids Res. | volume = 31 | issue = 7 | pages = 1805–12 | date=April 2003 | pmid = 12654995 | pmc = 152790 | doi = 10.1093/nar/gkg274 }} and bibliographical references. Note: When alphabetizing, enzymes are first ordered alphabetically by the acronyms (everything before the roman numeral); then enzymes of a given acronym are ordered alphabetically by the roman numeral, treating the numeral as a number and not a string of letters. This helps keep the entries ordered hierarchically while also alphabetic.(Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.{{cite book|vauthors=Jeremy MB, John LT, Lubert S | title=Biochemistry | publisher=W. H. Freeman | location=San Francisco | year=2002 | isbn=0-7167-4684-0 | chapter=3. Protein Structure and Function|chapter-url=https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=books&doptcmdl=GenBookHL&term=stryer%5Bbook%5D+AND+215168%5Buid%5D&rid=stryer.chapter.280}}{{cite journal |author=Anfinsen C.B. |year=1973 | title=Principles that Govern the Folding of Protein Chains |journal=Science| pages=223–30| pmid=4124164| doi=10.1126/science.181.4096.223|volume=181|issue=4096|bibcode=1973Sci...181..223A }}
  • REBASE Number: Number used to identify restriction enzymes in the [http://rebase.neb.com REBASE restriction enzyme database]. This database includes important information about the enzyme such as Recognition sequence, source, and Isoschizomers, as well as other data, such as the commercial suppliers of the enzyme.
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Displays the cut site and pattern and products of the cut. The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site.{{cite journal | vauthors = Kessler C, Manta V | title = Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3) | journal = Gene | volume = 92 | issue = 1–2 | pages = 1–248 | date=August 1990 | pmid = 2172084 | doi = 10.1016/0378-1119(90)90486-B }}{{cite journal | vauthors = Pingoud A, Jeltsch A | title = Structure and function of type II restriction endonucleases | journal = Nucleic Acids Res. | volume = 29 | issue = 18 | pages = 3705–27 | date=September 2001 | pmid = 11557805 | pmc = 55916 | doi = 10.1093/nar/29.18.3705 }}
  • Isoschizomers and neoschizomers: An isoschizomer is a restriction enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8–10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None as of [date]" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists, as in this not listed enzyme: {{pad|0.1em}}Abc123I{{pad|0.1em}}

Whole list navigation

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pre3=Ba-Bc | pre3link=List of restriction enzyme cutting sites: Ba-Bc#Whole list navigation|

pre4=Bd-Bp | pre4link=List of restriction enzyme cutting sites: Bd-Bp#Whole list navigation|

pre5=Bsa-Bso | pre5link=List of restriction enzyme cutting sites: Bsa-Bso#Whole list navigation|

pre6=Bsp | pre6link=List of restriction enzyme cutting sites: Bsp-Bss#Whole list navigation|

pre7=Bsr-Bss | pre7link=List of restriction enzyme cutting sites: Bsp-Bss#Bsr – Bss|

pre8=Bst | pre8link=List of restriction enzyme cutting sites: Bst-Bv#Whole list navigation|

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custom1=– External links | custom1link=List of restriction enzyme cutting sites#External links|

custom2=See also | custom2link=List of restriction enzyme cutting sites#See also|}}

Restriction enzymes

=A=

class="wikitable sortable" style="border:1px solid #aaa;"
Enzyme

! PDB code

! REBASE Number

! Source

! style="width:125px" | Recognition sequence

! style="width:550px" | Cut

! style="width:150px" | Isoschizomers

AaaI

|

|[http://rebase.neb.com/rebase/enz/AaaI.html 1]

|Acetobacter aceti

| 5' CGGCCG
3' GCCGGC

| 5' ---C {{pad|2em}} GGCCG--- 3'
3' ---GCCGG {{pad|2em}} C--- 5'

| BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII

AagI

|

|[http://rebase.neb.com/rebase/enz/AagI.html 4]

|Achromobacter agile

| 5' ATCGAT
3' TAGCTA

| 5' ---AT {{pad|2em}} CGAT--- 3'
3' ---TAGC {{pad|2em}} TA--- 5'

| BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI

AanI

|

|[http://rebase.neb.com/rebase/enz/AanI.html 15358]

|Arthrobacter aurescens RFL2

| 5' TTATAA
3' AATATT

| 5' ---TTA {{pad|2em}} TAA--- 3'
3' ---AAT {{pad|2em}} ATT--- 5'

| PsiI, Csp1BORF7380P

AarI{{Cite journal|vauthors=Grigaite R, Maneliene Z, Janulaitis A | title = AarI, a restriction endonuclease from Arthrobacter aurescens SS2-322, which recognizes the novel non-palindromic sequence 5'-CACCTGC(N)4/8-3' | journal = Nucleic Acids Res | volume = 30 | issue = 21 | pages = 123e–123 | date = November 2002 | pmid = 12409482 | pmc = 135850 | doi = 10.1093/nar/gnf122}}

|

|[http://rebase.neb.com/rebase/enz/AarI.html 2892]

|Arthrobacter aurescens SS2-322

| 5' CACCTGC
3' GTGGACG

| 5' ---CACCTGCN3N {{pad|2em}} NNNN--- 3'
3' ---GTGGACGN3NNNNN {{pad|2em}} --- 5'

|PaqCI

AasI

|

|[http://rebase.neb.com/rebase/enz/AasI.html 5465]

|Arthrobacter aurescens RFL3

| 5' GACN6GTC
3' CTGN6CAG

| 5' ---GACNNNN {{pad|2em}} NNGTC--- 3'
3' ---CTGNN {{pad|2em}} NNNNCAG--- 5'

| DrdI, DseDI

AatI{{Cite journal|vauthors=Sugisaki H, Maekawa Y, Kanazawa S, Takanami M | title = New restriction endonucleases from Acetobacter aceti and Bacillus aneurinolyticus | journal = Nucleic Acids Res | volume = 10 | issue = 19 | pages = 5747–52 | date = October 1982 | pmid = 6292849 | doi = 10.1093/nar/10.19.5747 | pmc=320926}}

|

|[http://rebase.neb.com/rebase/enz/AatI.html 6]

|Acetobacter aceti

| 5' AGGCCT
3' TCCGGA

| 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5'

| AspMI, Eco147I, {{pad|0.1em}}Eco1524I,{{pad|0.1em}} PceI, Pme55I, SarI, Sru30DI, SteI

AatII{{Cite journal|vauthors=Nwankwo DO, Maunus RE, Xu S | title = Cloning and expression of AatII restriction-modification system in Escherichia coli | journal = Gene | volume = 185 | issue = 1 | pages = 105–9 | date = January 1997 | pmid = 9034320 | doi = 10.1016/S0378-1119(96)00641-5 }}{{Cite journal| author = Roberts RJ| title = Restriction and modification enzymes and their recognition sequences | journal = Nucleic Acids Res | volume =11 | issue = 1| pages = r135–69| date = January 1983 | pmid =6306557 | pmc=325705}}

|

|[http://rebase.neb.com/rebase/enz/AatII.html 7]

|Acetobacter aceti

| 5' GACGTC
3' CTGCAG

| 5' ---GACGT {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} TGCAG--- 5'

| Ssp5230I, ZraI

AauI

|

|[http://rebase.neb.com/rebase/enz/AauI.html 3010]

|Arthrobacter aurescens

| 5' TGTACA
3' ACATGT

| 5' ---T {{pad|2em}} GTACA--- 3'
3' ---ACATG {{pad|2em}} T--- 5'

| {{pad|0.1em}}BsmRI,{{pad|0.1em}} Bsp1407I, BsrGI, BstAUI, Ssp4800I, SspBI

AbaI

|

|[http://rebase.neb.com/rebase/enz/AbaI.html 3029]

|Azospirillum brasilense UQ 1796

| 5' TGATCA
3' ACTAGT

| 5' ---T {{pad|2em}} GATCA--- 3'
3' ---ACTAG {{pad|2em}} T--- 5'

| BclI, BsiQI, BspXII, BstT7I, FbaI, Ksp22I, {{pad|0.1em}}ParI{{pad|0.1em}}

AbeI

|

|[http://rebase.neb.com/rebase/enz/AbeI.html 3097]

|Azotobacter beijerinckii Slo 54-028

| 5' CCTCAGC
3' GGAGTCG

| 5' ---CC {{pad|2em}} TCAGC--- 3'
3' ---GGAGT {{pad|2em}} CG--- 5'

| BbvCI

AbrI

|

|[http://rebase.neb.com/rebase/enz/AbrI.html 8]

|Azospirillum brasilense

| 5' CTCGAG
3' GAGCTC

| 5' ---C {{pad|2em}} TCGAG--- 3'
3' ---GAGCT {{pad|2em}} C--- 5'

| BluI, BspAAI, BssHI, EscI, PaeR7I, SciI, Sfr274I, Sol10179I, StrI, TliI

AbsI

|

|[http://rebase.neb.com/rebase/enz/AbsI.html 14594]

|Arthrobacter sp. 7M06

| 5' CCTCGAGG
3' GGAGCTCC

| 5' ---CC {{pad|2em}} TCGAGG--- 3'
3' ---GGAGCT {{pad|2em}} CC--- 5'

|None as of 4 Apr. 2019

AccI{{Cite journal| vauthors = Nishigaki K, Kaneko Y, Wakuda H, Husimi Y, Tanaka T | title = Type II restriction endonucleases cleave single-stranded DNAs in general | journal = Nucleic Acids Res | volume = 13 | issue = 16 | pages = 5747–60 |date=August 1985 | pmid = 2994012 | doi = 10.1093/nar/13.16.5747 | pmc=321909}}{{Cite journal| vauthors = Shenoy S, Daigle K, Ehrlich KC, Gehrke CW, Ehrlich M| title = Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs | journal = Nucleic Acids Res | volume = 14 | issue = 11 | pages = 4407–20 |date=June 1986 | pmid = 3012472 | pmc=311455}}{{Cite journal| vauthors = Nelson M, Christ C, Schildkraut I | title = Alteration of apparent restriction endonuclease recognition specificities by DNA methylases | journal = Nucleic Acids Res | volume = 12 | issue = 13 | pages = 5165–73 |date=July 1984 | pmid = 6087274 | doi = 10.1093/nar/12.13.5165 | pmc=318911}}{{Cite journal| vauthors = Nelson PS, Papas TS, Schweinfest CW | title = Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios | journal = Nucleic Acids Res | volume = 21 | issue = 3 | pages = 681–6 |date=February 1993 | pmid = 8441677 | doi = 10.1093/nar/21.3.681 | pmc=309169}}

|

|[http://rebase.neb.com/rebase/enz/AccI.html 18]

|Acinetobacter calcoaceticus

| 5' GTMKAC
3' CAKMTG

| 5' ---GT {{pad|2em}} MKAC--- 3'
3' ---CAKM {{pad|2em}} TG--- 5'

| FblI, XmiI

AccII{{Cite journal| vauthors = Kita K, Hiraoka N, Kimizuka F, Obayashi A | title = Determination of the cleavage site of restriction enzyme, AccII, using synthetic oligonucleotide | journal = Agric Biol Chem | volume = 48 | issue = 2 | pages = 531–2 | year = 1984 | doi=10.1271/bbb1961.48.531| doi-access = free }}

|

|[http://rebase.neb.com/rebase/enz/AccII.html 19]

|Acinetobacter calcoaceticus

| 5' CGCG
3' GCGC

| 5' ---CG {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} GC--- 5'

| Bsh1236I, Bsp50I, BstFNI, BstUI, MvnI, SelI, BceBI, BepI, Bpu95I

AccIII{{Cite journal| vauthors = Kita K, Hiraoka N, Oshima A, Kadonishi S, Obayashi A| title = AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus | journal =Nucleic Acids Res | volume =13 | issue =24 | pages = 8685–94|date=December 1985 | pmid = 3001647 | doi = 10.1093/nar/13.24.8685 | pmc=318944}}

|

|[http://rebase.neb.com/rebase/enz/AccIII.html 20]

|Acinetobacter calcoaceticus

| 5' TCCGGA
3' AGGCCT

| 5' ---T {{pad|2em}} CCGGA--- 3'
3' ---AGGCC {{pad|2em}} T--- 5'

| Aor13HI, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI

Acc16I

|

|[http://rebase.neb.com/rebase/enz/Acc16I.html 2638]

|Acinetobacter calcoaceticus 16

| 5' TGCGCA
3' ACGCGT

| 5' ---TGC {{pad|2em}} GCA--- 3'
3' ---ACG {{pad|2em}} CGT--- 5'

| AosI, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I

Acc36I{{Cite journal| vauthors = Bath AJ, Milsom SE, Gormley NA, Halford SE | title = Many type IIs restriction endonucleases interact with two recognition sites before cleaving DNA | journal = J Biol Chem| volume = 277| issue = 6 | pages = 4024–33 |date=February 2002 | pmid = 11729187 | doi = 10.1074/jbc.M108441200| doi-access = free }}

|

|[http://rebase.neb.com/rebase/enz/Acc36I.html 4162]

|Acinetobacter calcoaceticus 36

| 5' ACCTGC
3' TGGACG

| 5' ---ACCTGCN3N {{pad|2em}} NNNN--- 3'
3' ---TGGACGN3NNNNN {{pad|2em}} --- 5'

| BfuAI, BspMI, BveI

Acc65I{{Cite journal| vauthors = Prichodko GG, Rechnukova NI, Repin VE, Degtyarev SK| title = Determination of substrate specificity of restriction endonuclease Acc65I | journal = Sib Biol J | volume = 1| pages = 59–60| year = 1991 }}

|

|[http://rebase.neb.com/rebase/enz/Acc65I.html 14]

|Acinetobacter calcoaceticus 65

| 5' GGTACC
3' CCATGG

| 5' ---G {{pad|2em}} GTACC--- 3'
3' ---CCATG {{pad|2em}} G--- 5'

| AhaB8I, Asp718I, KpnI, SthI

Acc113I

|

|[http://rebase.neb.com/rebase/enz/Acc113I.html 2665]

|Acinetobacter calcoaceticus 113

| 5' AGTACT
3' TCATGA

| 5' ---AGT {{pad|2em}} ACT--- 3'
3' ---TCA {{pad|2em}} TGA--- 5'

| AssI, {{pad|0.1em}}BmcAI, Bpa34I,{{pad|0.1em}} DpaI, Eco255I, RflFII, ScaI, ZrmI

AccB1I

|

|[http://rebase.neb.com/rebase/enz/AccB1I.html 15]

|Acinetobacter calcoaceticus B1

| 5' GGYRCC
3' CCRYGG

| 5' ---G {{pad|2em}} GYRCC--- 3'
3' ---CCRYG {{pad|2em}} G--- 5'

| BanI, BbvBI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I

AccB2I

|

|[http://rebase.neb.com/rebase/enz/AccB2I.html 2666]

|Acinetobacter calcoaceticus B2

| 5' RGCGCY
3' YCGCGR

| 5' ---RGCGC {{pad|2em}} Y--- 3'
3' ---Y {{pad|2em}} CGCGR--- 5'

| {{pad|0.1em}}BfoI,{{pad|0.1em}} Bme142I, Bsp143II, BstH2I, HaeII, LpnI

AccB7I{{Cite journal| title =Screening of strains producing restriction endonucleases in Lake Baikal | journal = Izv Sib Otd Akad Nauk SSSR| volume = 1 | pages = 35–37| year = 1990}}

|

|[http://rebase.neb.com/rebase/enz/AccB7I.html 16]

|Acinetobacter calcoaceticus B7

| 5' CCAN5TGG
3' GGTN5ACC

| 5' ---CCANNNN {{pad|2em}} NTGG--- 3'
3' ---GGTN {{pad|2em}} NNNNACC--- 5'

| AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I

AccBSI{{Cite journal| vauthors = Abdurashitov MA, Kiseleva EV, Miakisheva TV, Dedkov VS, Shevchenko AV, Degtiarev CK | title = AccBSI – a novel restriction endonuclease from Acinetobacter calcoaceticus BS (Article in russian) | journal = Prikl Biokhim Mikrobiol | volume = 33| issue = 5| pages = 556–8 (Translation into english in pp. 496–8) |date=September–October 1997| pmid = 9441298}}

|

|[http://rebase.neb.com/rebase/enz/AccBSI.html 2733]

|Acinetobacter calcoaceticus BS

| 5' CCGCTC
3' GGCGAG

| 5' ---CCG {{pad|2em}} CTC--- 3'
3' ---GGC {{pad|2em}} GAG--- 5'

| BsrBI, BstD102I, Bst31NI, MbiI

AccEBI

|

|[http://rebase.neb.com/rebase/enz/AccEBI.html 17]

|Acinetobacter calcoaceticus EBF 65/65

| 5' GGATCC
3' CCTAGG

| 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5'

| AsiI, BamHI, Bce751I, Bsp98I, BspAAIII, CelI, OkrAI, Uba4009I

AceI

|

|[http://rebase.neb.com/rebase/enz/AceI.html 2571]

|Anabaena cedrorum

| 5' GCWGC
3' CGWCG

| 5' ---G {{pad|2em}} CWGC--- 3'
3' ---CGWC {{pad|2em}} G--- 5'

| ApeKI, SuiI, Taq52I, TseI

AceII

|

|[http://rebase.neb.com/rebase/enz/AceII.html 2570]

|Anabaena cedrorum

| 5' GCTAGC
3' CGATCG

| 5' ---GCTAG {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} GATCG--- 5'

|AsuNHI, BmtI, {{pad|0.1em}}BspOI,{{pad|0.1em}}, LlaG2I, NheI, PstNHI

AceIII

|

|[http://rebase.neb.com/rebase/enz/AceIII.html 2569]

|Anabaena cedrorum

| 5' CAGCTC
3' GTCGAG

| 5' ---CAGCTCN5N {{pad|2em}} NNNN--- 3'
3' ---GTCGAGN5NNNNN {{pad|2em}} --- 5'

|— None as of May 2010 —

AciI{{Cite journal| vauthors = Polisson C, Morgan RD | title = AciI, a unique restriction endonuclease from Arthrobacter citreus which recognizes 5' CCGC3' | journal =Nucleic Acids Res | volume = 18| issue =19 | pages = 5911|date=October 1990| pmid = 2170952 | doi = 10.1093/nar/18.19.5911 | pmc=332357}}

|

|[http://rebase.neb.com/rebase/enz/AciI.html 21]

|Arthrobacter citreus

| 5' CCGC
3' GGCG

| 5' ---C {{pad|2em}} CGC--- 3'
3' ---GGC {{pad|2em}} G--- 5'

| SsiI

AclI{{Cite journal| vauthors = Degtyarev SK, Abdurashitov MA, Kolyhalov AA, Rechkunova NI | title =AclI, a new restriction endonuclease from Acinetobacter calcoaceticus recognizing 5'-AA^CGTT-3' | journal = Nucleic Acids Res | volume = 20| issue = 14| pages = 3787|date=July 1992| pmid = 1322531 | doi = 10.1093/nar/20.14.3787 | pmc=334039}}

|

|[http://rebase.neb.com/rebase/enz/AclI.html 2181]

|Acinetobacter calcoaceticus M4

| 5' AACGTT
3' TTGCAA

| 5' ---AA {{pad|2em}} CGTT--- 3'
3' ---TTGC {{pad|2em}} AA--- 5'

| Psp1406I

AclNI

|

|[http://rebase.neb.com/rebase/enz/AclNI.html 2667]

|Acinetobacter calcoaceticus N20

| 5' ACTAGT
3' TGATCA

| 5' ---A {{pad|2em}} CTAGT--- 3'
3' ---TGATC {{pad|2em}} A--- 5'

| AhlI, BcuI, SpeI

AclWI

|

|[http://rebase.neb.com/rebase/enz/AclWI.html 2668]

|Acinetobacter calcoaceticus W2131

| 5' GGATC
3' CCTAG

| 5' ---GGATCN3N {{pad|2em}} N--- 3'
3' ---CCTAGN3NN {{pad|2em}} --- 5'

| AlwI, BinI, {{pad|0.1em}}BpuFI, BsrWI,{{pad|0.1em}} BspPI, BstH9I, {{pad|0.1em}}Bst31TI,{{pad|0.1em}} EacI, {{pad|0.1em}}Ral8I{{pad|0.1em}}

AcoI

|

|[http://rebase.neb.com/rebase/enz/AcoI.html 11822]

|Acinetobacter calcoaceticus

| 5' YGGCCR
3' RCCGGY

| 5' ---Y {{pad|2em}} GGCCR--- 3'
3' ---RCCGG {{pad|2em}} Y--- 5'

| EaeI, Bfi89I, CfrI, EcoHK31I

AcpI

|

|[http://rebase.neb.com/rebase/enz/AcpI.html 22]

|Acidiphilium cryptum 25H

| 5' TTCGAA
3' AAGCTT

| 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5'

| Asp10HI, Bpu14I, Bsp119I, BstBI, Csp45I, FspII, MlaI, NspV, SspRFI

AcpII

|

|[http://rebase.neb.com/rebase/enz/AcpII.html 23]

|Acidiphilium cryptum 25H

| 5' CCAN5TGG
3' GGTN5ACC

| 5' ---CCANNNN {{pad|2em}} NTGG--- 3'
3' ---GGTN {{pad|2em}} NNNNACC--- 5'

| AccB7I, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I

AcrII

|

|[http://rebase.neb.com/rebase/enz/AcrII.html 25]

|Anabaenopsis circularis

| 5' GGTNACC
3' CCANTGG

| 5' ---G {{pad|2em}} GTNACC--- 3'
3' ---CCANTG {{pad|2em}} G--- 5'

| AspAI, Bse64I, BseT9I, BstEII, BstPI, Eco91I, EcoO65I, PspEI

AcsI{{Cite journal| vauthors = Degtyarev SK, Kolyhalov AA, Rechkunova NI, Abdurashitov MA| title = AcsI, a new restriction endonuclease from Arthrobacter citreus 310 recognizing 5'-Pu decreases AATTPy-3'| journal = Nucleic Acids Res| volume = 20| issue = 14| pages = 3789|date=July 1992| pmid = 1322532 | doi = 10.1093/nar/20.14.3789| pmc=334041}}

|

|[http://rebase.neb.com/rebase/enz/AcsI.html 2184]

|Arthrobacter citreus 310

| 5' RAATTY
3' YTTAAR

| 5' ---R {{pad|2em}} AATTY--- 3'
3' ---YTTAA {{pad|2em}} R--- 5'

| ApoI, {{pad|0.1em}}CfaI,{{pad|0.1em}} FsiI, XapI

AcuI

|

|[http://rebase.neb.com/rebase/enz/AcuI.html 5534]

|Acinetobacter calcoaceticus

| 5' CTGAAG
3' GACTTC

| 5' ---CTGAAGN13NNN {{pad|2em}} --- 3'
3' ---GACTTCN13N {{pad|2em}} NN--- 5'

| BspKT5I, Eco57I

AcvI

|

|[http://rebase.neb.com/rebase/enz/AcvI.html 5359]

|Aeromonas caviae

| 5' CACGTG
3' GTGCAC

| 5' ---CAC {{pad|2em}} GTG--- 3'
3' ---GTG {{pad|2em}} CAC--- 5'

| BbrPI, BcoAI, Eco72I, PmaCI, PmlI, PspCI

AcyI{{Cite journal| vauthors = de Waard A, Korsuize J, van Beveren CP, Maat J | title = A new sequence-specific endonuclease from Anabaena cylindrica | journal = FEBS Lett | volume =96 | issue =1 | pages =106–10 |date=December 1978| pmid = 103749 | doi = 10.1016/0014-5793(78)81072-2| doi-access = free | bibcode = 1978FEBSL..96..106D }}

|

|[http://rebase.neb.com/rebase/enz/AcyI.html 31]

|Anabaena cylindrica

| 5' GRCGYC
3' CYGCRG

| 5' ---GR {{pad|2em}} CGYC--- 3'
3' ---CYGC {{pad|2em}} RG--- 5'

| AhaII, AosII, BbiII, BsaHI, BstACI, Hin1I, HgiI, Hsp92I, Msp17I

AdeI

|

|[http://rebase.neb.com/rebase/enz/AdeI.html 2991]

|Alcaligenes denitrificans Ss 3-028

| 5' CACNNNGTG
3' GTGNNNCAC

| 5' ---CACNNN {{pad|2em}} GTG--- 3'
3' ---GTG {{pad|2em}} NNNCAC--- 5'

| BstIZ316I, DraIII

AeuI

|

|[http://rebase.neb.com/rebase/enz/AeuI.html 33]

|Achromobacter eurydice

| 5' CCWGG
3' GGWCC

| 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5'

| AglI, AjnI, BseBI, BstNI, BstOI, Bst2UI, EcoRII, SleI, SspAI

AfaI{{Cite journal| vauthors = Dou D, Inagati K, Kita K, Ohshima A, Hiraoka N, Kishimoto N, Sugio T, Tano T | title = Restriction endonuclease AfaI from Acidiphilium facilis, a new isoschizomer of RsaI: purification and properties | journal = Biochim Biophys Acta| volume = 1009| issue = 1| pages = 83–6|date=September 1989 | pmid = 2790034 | doi=10.1016/0167-4781(89)90082-1}}

|

|[http://rebase.neb.com/rebase/enz/AfaI.html 37]

|Acidiphilium facilis 28H

| 5' GTAC
3' CATG

| 5' ---GT {{pad|2em}} AC--- 3'
3' ---CA {{pad|2em}} TG--- 5'

|Csp6I, CviQI, CviRII, HpyBI, PabI, PlaAII, RsaI, RsaNI

Afa16RI

|

|[http://rebase.neb.com/rebase/enz/Afa16RI.html 34]

|Acidiphilium sp. 16R

| 5' CGATCG
3' GCTAGC

| 5' ---CGAT {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} TAGC--- 5'

| Afa22MI, BspCI, EagBI, MvrI, NblI, Ple19I, PvuI, Psu161I, RshI, XorII

Afa22MI

|

|[http://rebase.neb.com/rebase/enz/Afa22MI.html 35]

|Acidocella facilis

| 5' CGATCG
3' GCTAGC

| 5' ---CGAT {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} TAGC--- 5'

| Afa16RI, BspCI, EagBI, ErhB9I, NblI, Ple19I, Psu161I, PvuI, RshI

AfeI{{Cite journal| vauthors = Macícková-Cahová H, Hocek M | s2cid = 17925157| title = Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases| journal = Nucleic Acids Res| volume =37 | issue =22 | pages = 7612–22|date=December 2009| pmid = 19820117 | doi = 10.1093/nar/gkp845 | pmc=2794189}}

|

|[http://rebase.neb.com/rebase/enz/AfeI.html 2669]

|Alcaligenes faecalis T2774

| 5' AGCGCT
3' TCGCGA

| 5' ---AGC {{pad|2em}} GCT--- 3'
3' ---TCG {{pad|2em}} CGA--- 5'

| AitI, Aor51H, Eco47III, FunI

AfiI

|

|[http://rebase.neb.com/rebase/enz/AfiI.html 10489]

|Anoxybacillus flavithermus

| 5' CCN7GG
3' GGN7CC

| 5' ---CCNNNNN {{pad|2em}} NNGG--- 3'
3' ---GGNN {{pad|2em}} NNNNNCC--- 5'

| Bsc4I, BseLI, BslI, BflI, Bsc107I, BsiYI, Bst22I

AflI{{Cite journal| vauthors = Whitehead PR, Brown NL| title = Three restriction endonucleases from Anabaena flos-aquae| journal = J Gen Microbiol | volume = 131 | issue = 4 | pages = 951–8 |date=April 1985 | pmid = 2985742 | doi=10.1099/00221287-131-4-951| doi-access = free}}

|

|

|Anabaena flos-aquae

| 5' GGWCC
3' CCWGG

| 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5'

| Asp745I, BamNI, BcuAI, CauI, Csp68KI, DsaIV, Eco47I, SinI

AflII{{Cite journal| vauthors = Rowland GC, Lim PP, Glass RE | title = 'Stop-codon-specific' restriction endonucleases: their use in mapping and gene manipulation | journal = Gene | volume = 116 | issue = 1 | pages = 21–6 |date=July 1992 | pmid = 1628840 | doi = 10.1016/0378-1119(92)90624-X}}{{Cite journal| author = Roberts RJ | title = Restriction and modification enzymes and their recognition sequences | journal = Nucleic Acids Res | volume = 10| issue =5 | pages =r117–44 |date=March 1982 | pmid = 6280143 | doi = 10.1093/nar/10.5.1770 | pmc=320569}}

|

|[http://rebase.neb.com/rebase/enz/AflII.html 39]

|Anabaena flos-aquae

| 5' CTTAAG
3' GAATTC

| 5' ---C {{pad|2em}} TTAAG--- 3'
3' ---GAATT {{pad|2em}} C--- 5'

| BfrI, BspTI, Bst98I, BstAFI, BstPZ740I, Esp4I, MspCI, Vha464I

AflIII

|

|[http://rebase.neb.com/rebase/enz/AflIII.html 40]

|Anabaena flos-aquae

| 5' ACRYGT
3' TGYRCA

| 5' ---A {{pad|2em}} CRYGT--- 3'
3' ---TGYRC {{pad|2em}} A--- 5'

|{{pad|0.1em}}Asp90I{{pad|0.1em}}

AgeI{{Cite journal| vauthors =Yamada Y, Mizuno H, Sato H, Akagawa M, Yamasato K | title =A new restriction endonuclease from Agrobacterium gelatinovorum, a marine agrobacterium (AgeI) | journal =Agric Biol Chem | volume = 53| issue =6 | pages = 1747–9| year = 1989 | doi=10.1271/bbb1961.53.1747| doi-access =free }}

|

|[http://rebase.neb.com/rebase/enz/AgeI.html 42]

|Agrobacterium gelatinovorum

| 5' ACCGGT
3' TGGCCA

| 5' ---A {{pad|2em}} CCGGT--- 3'
3' ---TGGCC {{pad|2em}} A--- 5'

| AsiAI, AsiGI, BshTI, CsiAI, CspAI, PinAI

AglI{{cite journal | author = Sokolov NN | title = Search, isolation and study of restrictases | journal = Vestn Ross Akad Med Nauk | issue = 2 | pages = 47–51 | year = 1995 | pmid = 7756931 | language = ru}}

|

|[http://rebase.neb.com/rebase/enz/AglI.html 6652]

|Arthrobacter globiformis

| 5' CCWGG
3' GGWCC

| 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5'

| AeuI, Bse16I, Bse17I, Bse24I, BshGI, EcoRII, SleI, SniI, SslI

AgsI

|

|

|Agrococcus sp. 25

| 5' TTSAA
3' AASTT

| 5' ---TTS {{pad|2em}} AA--- 3'
5' ---AA {{pad|2em}} STT--- 3'

|None as of 4 May 2019

AhaI

|

|[http://rebase.neb.com/rebase/enz/AhaI.html 45]

|Alteromonas haloplanktis B8

| 5' CCSGG
3' GGSCC

| 5' ---CC {{pad|2em}} SGG--- 3'
3' ---GGS {{pad|2em}} CC--- 5'

| AseII, BcnI, {{pad|0.1em}}BpuMI,{{pad|0.1em}} CauII, Eco1831I, EcoHI, HgiS22I, NciI

AhaII

|

|

|Aphanothece halophytica

| 5' GRCGYC
3' CYGCRG

| 5' ---GR {{pad|2em}} CGYC--- 3'
3' ---CYGC {{pad|2em}} RG--- 5'

| AcyI, AstWI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiHII, PamII

AhaIII{{Cite journal| vauthors =Whitehead PR, Brown NL | title = AhaIII: A restriction endonuclease with a recognition sequence containing only A:T basepairs| journal =FEBS Lett | volume = 143| issue = 2| pages = 296–300|date=July 1982| pmid = 6288466 | doi = 10.1016/0014-5793(82)80120-8| doi-access = free| bibcode = 1982FEBSL.143..296W}}

|

|

|Aphanothece halophytica

| 5' TTTAAA
3' AAATTT

| 5' ---TTT {{pad|2em}} AAA--- 3'
3' ---AAA {{pad|2em}} TTT--- 5'

| DraI, PauAII, SruI

AhaB8I

|

|

|Aphanothece halophytica B8

| 5' GGTACC
3' CCATGG

| 5' ---G {{pad|2em}} GTACC--- 3'
3' ---CCATG {{pad|2em}} G--- 5'

| Acc65I, Asp718I, KpnI, SthI

AhdI{{Cite journal| vauthors = Marks P, McGeehan J, Wilson G, Errington N, Kneale G | title = Purification and characterisation of a novel DNA methyltransferase, M.AhdI| journal = Nucleic Acids Res | volume = 31| issue = 11| pages =2803–10 |date=June 2003| pmid = 12771207 | doi = 10.1093/nar/gkg399 | pmc=156732}}{{Cite journal| vauthors = Bogdanova E, Djordjevic M, Papapanagiotou I, Heyduk T, Kneale G, Severinov K | s2cid = 5338430 | title = Transcription regulation of the type II restriction-modification system AhdI | journal = Nucleic Acids Res| volume = 36| issue =5 | pages =1429–42 |date=March 2008 | pmid = 18203750 | doi = 10.1093/nar/gkm1116 | pmc=2275141}}

|

|

|Aeromonas hydrophila

| 5' GACN5GTC
3' CTGN5CAG

| 5' ---GACNNN {{pad|2em}} NNGTC--- 3'
3' ---CTGNN {{pad|2em}} NNNCAG--- 5'

| AspEI, {{pad|0.1em}}BmeRI,{{pad|0.1em}} BspOVI, DriI, Eam1105I, EclHKI, NruGI

AhlI

|

|[http://rebase.neb.com/rebase/enz/AhlI.html 4838]

|Alteromonas haloplanktis SP

| 5' ACTAGT
3' TGATCA

| 5' ---A {{pad|2em}} CTAGT--- 3'
3' ---TGATC {{pad|2em}} A--- 5'

| AclNI, BcuI, SpeI

AhyI

|

|[http://rebase.neb.com/rebase/enz/AhyI.html 49]

|Aeromonas hydrophila

| 5' CCCGGG
3' GGGCCC

| 5' ---C {{pad|2em}} CCGGG--- 3'
3' ---GGGCC {{pad|2em}} C--- 5'

| Cfr9I, CfrJ4I, EaeAI, EclRI, PaeBI, SmaI, TspMI, XmaI, XmaCI

AitI

|

|[http://rebase.neb.com/rebase/enz/AitI.html 54]

|Aquaspirillum itersonii

| 5' AGCGCT
3' TCGCGA

| 5' ---AGC {{pad|2em}} GCT--- 3'
3' ---TCG {{pad|2em}} CGA--- 5'

| AfeI, Aor51H, Eco47III, FunI

AjiI

|

|[http://rebase.neb.com/rebase/enz/AjiI.html 10919]

|Acinetobacter johnsonii RFL47

| 5' CACGTC
3' GTGCAG

| 5' ---CAC {{pad|2em}} GTC--- 3'
3' ---GTG {{pad|2em}} CAG--- 5'

|BmgBI, BtrI

AjnI

|

|[http://rebase.neb.com/rebase/enz/AjnI.html 7185]

|Acinetobacter johnsonii R2

| 5' CCWGG
3' GGWCC

| 5' --- {{pad|2em}} CCWGG--- 3'
3' ---GGWCC {{pad|2em}} --- 5'

|AorI, ApaORI, ApyI, EcoRII, MvaI, Psp6I, PspGI, SleI, SspAI

AjoI

|

|[http://rebase.neb.com/rebase/enz/AjoI.html 2604]

|Acinetobacter johnsonii 35

| 5' CTGCAG
3' GACGTC

| 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5'

| ApiI, BloHII, BspMAI, CfrA4I, CfuII, PstI, SalPI, SflI, Srl5DI

AjuI

|

|[http://rebase.neb.com/rebase/enz/AjuI.html 7740]

|Acinetobacter junii RFL46

| 5' GAAN7TTGG
3'CTTN7AACC

| 5' ---NNNNN {{pad|2em}} NN6GAAN7TTGGN5NNNNNN {{pad|2em}} --- 3'
3' --- {{pad|2em}} NNNNNNN6CTTN7AACCN5N {{pad|2em}} NNNNN--- 5'

|None as of 28 Oct. 2020

AleI

|

|[http://rebase.neb.com/rebase/enz/AleI.html 5791]

|Aureobacterium liquefaciens

| 5' CACN4GTG
3' GTGN4CAC

| 5' ---CACNN {{pad|2em}} NNGTG--- 3'
3' ---GTGNN {{pad|2em}} NNCAC--- 5'

| OliI

AlfI

|

|[http://rebase.neb.com/rebase/enz/AlfI.html 6110]

|Acinetobacter lwoffii BH 32

| 5' GCAN6TGC
3' CGTN6ACG

| 5' ---GCAN6TGCN9NNN {{pad|2em}} --- 3'
3' ---CGTN6ACGN9N {{pad|2em}} NN--- 5'

|None as of May 2010

AliI

|

|

|Acetobacter liquefaciens

| 5' GGATCC
3' CCTAGG

| 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5'

| AccEBI, BamHI, Bce751I, Bsp98I, BspAAIII, Nsp29132II, Pfl8I

AliAJI

|

|[http://rebase.neb.com/rebase/enz/AliAJI.html 59]

|Acetobacter liquefaciens AJ 2881

| 5' CTGCAG
3' GACGTC

| 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5'

| ApiI, Asp713I, BspMAI, CflI, CstI, MhaAI, PstI, SalPI, Sst12I, YenI

AloI{{Cite journal| vauthors = Cesnaviciene E, Petrusyte M, Kazlauskiene R, Maneliene Z, Timinskas A, Lubys A, Janulaitis A | title = Characterization of AloI, a restriction-modification system of a new type | journal = J Mol Biol| volume = 314| issue = 2| pages = 205–16|date=November 2001| pmid = 11718555 | doi = 10.1006/jmbi.2001.5049 }}{{Cite journal| vauthors = Jurenaite-Urbanaviciene S, Serksnaite J, Kriukiene E, Giedriene J, Venclovas C, Lubys A| title =Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains | journal = Proc Natl Acad Sci USA| volume = 104| issue = 25| pages = 10358–63|date=June 2007 | pmid = 17553965| doi = 10.1073/pnas.0610365104 | pmc=1965518| bibcode =2007PNAS..10410358J | doi-access =free }}

|

|[http://rebase.neb.com/rebase/enz/AloI.html 2812]

|Acinetobacter lwoffii Ks 4-8

| 5' GAACN6TCC
3' CTTGN6AGG

| 5' ---GAACN6TCCN6NNNNNN {{pad|2em}} --- 3'
3' ---CTTGN6AGGN6N {{pad|2em}} NNNNN--- 5'

|None as of May 2010

AluI{{Cite journal| vauthors =Blakesley RW, Wells RD | s2cid =24619091 | title ='Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases | journal = Nature| volume = 257| issue = 5525| pages = 421–2|date=October 1975| pmid = 809717 | doi = 10.1038/257421a0 | bibcode =1975Natur.257..421B }}{{Cite journal| vauthors = Roberts RJ, Myers PA, Morrison A, Murray K| title =A specific endonuclease from Arthrobacter luteus | journal = J Mol Biol| volume = 102| issue = 1| pages = 157–65|date=March 1976| pmid = 1271462 | doi = 10.1016/0022-2836(76)90079-6 }}{{Cite journal| vauthors = Greene PJ, Heyneker HL, Bolivar F, Rodriguez RL, Betlach MC, Covarrubias AA, Backman K, Russel DJ, Tait R, Boyer HW| title = A general method for the purification of restriction enzymes| journal = Nucleic Acids Res | volume = 5 | issue = 7| pages = 2373–80|date=July 1978| pmid = 673857 | doi = 10.1093/nar/5.7.2373 | pmc=342170}}{{Cite journal| vauthors = Huang LH, Farnet CM, Ehrlich KC, Ehrlich M | title = Digestion of highly modified bacteriophage DNA by restriction endonucleases | journal = Nucleic Acids Res | volume = 10 | issue = 5 | pages = 1579–91 |date=March 1982 | pmid = 6280151 | doi = 10.1093/nar/10.5.1579 | pmc=320551}}

|

|[http://rebase.neb.com/rebase/enz/AluI.html 61]

|Arthrobacter luteus

| 5' AGCT
3' TCGA

| 5' ---AG {{pad|2em}} CT--- 3'
3' ---TC {{pad|2em}} GA--- 5'

| AluBI, MltI

AluBI

|

|[http://rebase.neb.com/rebase/enz/AluBI.html 16189]

|Arthrobacter luteus B

| 5' AGCT
3' TCGA

| 5' ---AG {{pad|2em}} CT--- 3'
3' ---TC {{pad|2em}} GA--- 5'

|AluI, MltI

AlwI

|

|[http://rebase.neb.com/rebase/enz/AlwI.html 65]

|Acinetobacter lwoffii

| 5' GGATC
3' CCTAG

| 5' ---GGATCN3N {{pad|2em}} N--- 3'
3' ---CCTAGN3NN {{pad|2em}} --- 5'

| AclWI, BinI, BspPI, {{pad|0.1em}}BsrWI,{{pad|0.1em}} BstH9I, {{pad|0.1em}}BthII, Bth617I,{{pad|0.1em}} EacI

Alw21I

|

|

|Acinetobacter lwoffii RFL21

| 5' GWGCWC
3' CWCGWG

| 5' ---GWGCW {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} WCGWG--- 5'

| AspHI, Bbv12I, Bsh45I, BsiHKAI,{{pad|0.1em}}Bsm6I,{{pad|0.1em}} HgiAI, MspV281I

Alw26I{{Cite journal| vauthors = Bitinaite J, Maneliene Z, Menkevicius S, Klimasauskas S, Butkus V, Janulaitis A| title =Alw26I, Eco31I and Esp3I—type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA | journal = Nucleic Acids Res| volume = 20| issue = 19| pages = 4981–5|date=October 1992| pmid = 1408816 | doi = 10.1093/nar/20.19.4981 | pmc=334273}}

|

|[http://rebase.neb.com/rebase/enz/Alw26I.html 63]

|Acinetobacter lwoffii RFL26

| 5' GTCTC
3' CAGAG

| 5' ---GTCTCN {{pad|2em}} NNNN--- 3'
3' ---CAGAGNNNNN {{pad|2em}} --- 5'

| {{pad|0.1em}}BcoDI, BscQII, {{pad|0.1em}} BsmAI, BsoMAI, {{pad|0.1em}}BstMAI{{pad|0.1em}}

Alw44I

|

|

|Acinetobacter lwoffii RFL44

| 5' GTGCAC
3' CACGTG

| 5' ---G {{pad|2em}} TGCAC--- 3'
3' ---CACGT {{pad|2em}} G--- 5'

| ApaLI, SnoI, VneI

AlwNI{{Cite journal| vauthors =Morgan RD, Dalton M, Stote R | title = A unique type II restriction endonuclease from Acinetobacter lwoffi N| journal = Nucleic Acids Res| volume = 15| issue = 17| pages = 7201|date=September 1987| pmid = 2821501 | doi =10.1093/nar/15.17.7201 | pmc=306225}}

|

|

|Acinetobacter lwoffii N

| 5' CAGNNNCTG
3' GTCNNNGAC

| 5' ---CAGNNN {{pad|2em}} CTG--- 3'
3' ---GTC {{pad|2em}} NNNGAC--- 5'

| CaiI

AlwXI

|

|[http://rebase.neb.com/rebase/enz/AlwXI.html 67]

|Acinetobacter lwoffii X

| 5' GCAGC
3' CGTCG

| 5' ---GCAGCN7N {{pad|2em}} NNNN--- 3'
3' ---CGTCGN7NNNNN {{pad|2em}} --- 5'

| BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I

Ama87I

|

|

|Alteromonas macleodii 87

| 5' CYCGRG
3' GRGCYC

| 5' ---C {{pad|2em}} YCGRG--- 3'
3' ---GRGCY {{pad|2em}} C--- 5'

| AquI, AvaI, {{pad|0.1em}}BmeT110I,{{pad|0.1em}} BsiHKCI, BsoBI, Eco88I, Nli3877I, NspSAI

AmaCSI

|

|[http://rebase.neb.com/rebase/enz/AmaCSI.html 26714]

|Arthrospira maxima CS-328

| 5' GCTCCA
3' CGAGGT

| 5' ---GCTCCAN8NNN {{pad|2em}} --- 3'
3' ---CGAGGTN8N {{pad|2em}} NN--- 5'

|None as of 4 May 2018

AocI

|

|[http://rebase.neb.com/rebase/enz/AocI.html 74]

|Anabaena sp.

| 5' CCTNAGG
3' GGANTCC

| 5' ---CC {{pad|2em}} TNAGG--- 3'
3' ---GGANT {{pad|2em}} CC--- 5'

| AyI, Bse21I, Bsu36I, Eco81I, Lmu60I, OaNI, MstII, SauI, SshAI

AocII

|

|

|Anabaena sp.

| 5' GDGCHC
3' CHCGDG

| 5' ---GDGCH {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} HCGDG--- 5'

| BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII, SduI

AorI

|

|[http://rebase.neb.com/rebase/enz/AorI.html 77]

|Acetobacter aceti orleanensis

| 5' CCWGG
3' GGWCC

| 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5'

| BseBI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, Sth117I, Tai

Aor13HI{{Cite journal| vauthors =Inagaki K, Hikita T, Yanagidani S, Nomura Y, Kishimoto N, Tano T, Tanaka H | title =Restriction endonuclease Aor13HI from Acidiphilium organovorum 13H, a new isoschizomer of BspMII: Purification and characterization | journal = Biosci Biotechnol Biochem| volume =57 | issue = 10| pages = 1716–21|date=October 1993| pmid = 7764267 | doi = 10.1271/bbb.57.1716}}

|

|

|Acidiphilium organovorum 13H

| 5' TCCGGA
3' AGGCCT

| 5' ---T {{pad|2em}} CCGGA--- 3'
3' ---AGGCC {{pad|2em}} T--- 5'

| AccIII, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI

Aor51HI{{Cite journal| vauthors = Sagawa H, Takagi M, Nomura Y, Inagaki K, Tano T, Kishimato N, Kotani H, Nakajima K | title = Isolation and identification of restriction endonuclease Aor51HI from Acidiphilium organovorum 51H | journal = Nucleic Acids Res | volume = 20| issue = 2| pages = 365|date=January 1992 | pmid = 1741262 | doi = 10.1093/nar/20.2.365 | pmc=310379}}

|

|[http://rebase.neb.com/rebase/enz/Aor51HI.html 76]

|Acidiphilium organovorum 51H

| 5' AGCGCT
3' TCGCGA

| 5' ---AGC {{pad|2em}} GCT--- 3'
3' ---TCG {{pad|2em}} CGA--- 5'

| AfeI, AitI, Eco47III, FunI

AosI

|

|

|Anabaena oscillarioides

| 5' TGCGCA
3' ACGCGT

| 5' ---TGC {{pad|2em}} GCA--- 3'
3' ---ACG {{pad|2em}} CGT--- 5'

| Acc16I, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I

AosII

|

|

|Anabaena oscillarioides

| 5' GRCGYC
3' CYGCRG

| 5' ---GR {{pad|2em}} CGYC--- 3'
3' ---CYGC {{pad|2em}} RG--- 5'

| AcyI, AstWI, BbiII, BsaHI, BstACI, Hin1I, HgiI, HgiGI, Msp17I, PamII

ApaI{{Cite journal| title =A new restriction endonuclease from Acetobacter pasteurianus | journal =Nucleic Acids Res | volume =11 | issue =13 | pages = 4409–15|date=July 1983 | pmid = 6306590| doi = 10.1093/nar/11.13.4409| pmc=326055 | vauthors=Seurinck J, Van, de Voorde A, Van Montagu M}}

|

|

|Acetobacter pasteurianus pasteurianus

| 5' GGGCCC
3' CCCGGG

| 5' ---GGGCC {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} CCGGG--- 5'

| Bsp120I, PpeI, PspOMI

ApaBI{{Cite journal| vauthors = Grones J, Turna J | title = Some properties of restriction endonuclease ApaBI from Acetobacter pasteurianus | journal = Biochim Biophys Acta | volume = 1162| issue = 3| pages =323–5 |date=March 1993| pmid = 8457597| doi = 10.1016/0167-4838(93)90297-5}}

|

|

|Acetobacter pasteurianus B

| 5' GCAN5TGC
3' CGTN5ACG

| 5' ---GCANNNNN {{pad|2em}} TGC--- 3'
3' ---CGT {{pad|2em}} NNNNNACG--- 5'

| BstAPI

ApaCI

|

|

|Acetobacter pasteurianus C

| 5' GGATCC
3' CCTAGG

| 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5'

| AliI, BamHI, BnaI, Bsp4009I, BstI, NspSAIV, Pfl8I, SolI, SurI

ApaLI{{Cite journal| vauthors =Xu SY, Xiao JP, Ettwiller L, Holden M, Aliotta J, Poh CL, Dalton M, Robinson DP, Petronzio TR, Moran L, Ganatra M, Ware J, Slatko B, Benner J | s2cid = 2275352 | title = Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli | journal =Mol Gen Genet | volume = 260| issue = 2–3| pages = 226–31|date=Nov 1998| pmid = 9862476 | doi =10.1007/s004380050890 }}{{Cite journal| vauthors = Xia Y, Van Etten JL, Dobos P, Ling YY, Krell PJ | title =Adenine DNA methyltransferase M.CviRI expression accelerates apoptosis in baculovirus-infected insect cells | journal = Virology| volume = 196| issue = 2| pages = 817–24|date=October 1993| pmid = 8372450| doi = 10.1006/viro.1993.1539 }}

|

|

|Acetobacter pasteurianus

| 5' GTGCAC
3' CACGTG

| 5' ---G {{pad|2em}} TGCAC--- 3'
3' ---CACGT {{pad|2em}} G--- 5'

| Alw44I, SnoI, VneI

ApaORI

|

|[http://rebase.neb.com/rebase/enz/ApaORI.html 86]

|Acetobacter pasteurianus

| 5' CCWGG
3' GGWCC

| 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5'

|AjnI, BstNI, BstOI, Bst1I, Bst2I, Fsp1604I, MvaI, Psp6I, PspGI

ApeKI{{Cite journal| vauthors =Kawarabayasi Y, Hino Y, Horikawa H, Yamazaki S, Haikawa Y, Jin-no K, Takahashi M, Sekine M, Baba S, Ankai A, Kosugi H, Hosoyama A, Fukui S, Nagai Y, Nishijima K, Nakazawa H, Takamiya M, Masuda S, Funahashi T, Tanaka T, Kudoh Y, Yamazaki J, Kushida N, Oguchi A, Aoki K, Kubota K, Nakamura Y, Nomura N, Sako Y, Kikuchi H | title =Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1 | journal = DNA Res | volume = 6 | issue = 2| pages = 83–101, 145–52|date=April 1999 | pmid = 10382966 | doi = 10.1093/dnares/6.2.83| doi-access = free }}

|

|

|Aeropyrum pernix K1

| 5' GCWGC
3' CGWCG

| 5' ---G {{pad|2em}} CWGC--- 3'
3' ---CGWC {{pad|2em}} G--- 5'

| AceI, SuiI, Taq52I, TseI

ApiI

|

|[http://rebase.neb.com/rebase/enz/ApiI.html 2315]

|Arthrobacter picolinophilus

| 5' CTGCAG
3' GACGTC

| 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5'

| Asp713I, Bsp63I, CfrA4I, PaePI, Pfl21I, Psp23I, PstI, SflI, Sst12I

ApoI{{Cite journal| vauthors = Polisson C, Robinson D| title = ApoI, a unique restriction endonuclease from Arthrobacter protophormiae which recognizes 5' RAATTY-3' | journal = Nucleic Acids Res. | volume =20 |issue = 11| pages = 2888|date=June 1992 | pmid = 1614876 | doi = 10.1093/nar/20.11.2888 | pmc=336940}}

|

|

|Arthrobacter protophormiae

| 5' RAATTY
3' YTTAAR

| 5' ---R {{pad|2em}} AATTY--- 3'
3' ---YTTAA {{pad|2em}} R--- 5'

| AcsI,{{pad|0.1em}}CfaI,{{pad|0.1em}} FsiI, XapI, EcoRI

AplI

|

|[http://rebase.neb.com/rebase/enz/AplI.html 25962]

|Arthrospira platensis NIES-39

| 5' CTGCAG
3' GACGTC

| 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5'

|See [http://rebase.neb.com/cgi-bin/isoget?AplI this site] for a complete list.

ApyI

|

|[http://rebase.neb.com/rebase/enz/ApyI.html 93]

|Arthrobacter pyridinolis

| 5' CCWGG
3' GGWCC

| 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5'

| BciBII, BptI, BseBI, BstNI, BstOI, Bst2UI, Bst38I, Bst100I, MvaI

ApyPI

|

|[http://rebase.neb.com/rebase/enz/ApyPI.html 14756]

|Arcanobacterium pyogenes

| 5' ATCGAC
3' TAGCTG

| 5' ---ATCGACN17NNN {{pad|2em}} --- 3'
3' ---TAGCTGN17N {{pad|2em}} NN--- 5'

|None as of 4 August 2019

AquI

|

|

|Agmenellum quadruplicatum PR-6

| 5' CYCGRG
3' GRGCYC

| 5' ---C {{pad|2em}} YCGRG--- 3'
3' ---GRGCY {{pad|2em}} C--- 5'

| BcoI, {{pad|0.1em}}BmeT110I,{{pad|0.1em}} BsmAI, BsoMAI, BsoBI, BstSI, Eco27kI, OfoI

AquII

|

|[http://rebase.neb.com/rebase/enz/AquII.html 20238]

|Agmenellum quadruplicatum PR-6

| 5' GCCGNAC
3' CGGCNTG

| 5' ---GCCGNACN17NNN {{pad|2em}} --- 3'
3' ---CGGCNTGN17N {{pad|2em}} NN--- 5'

|None as of 4 August 2019

AquIII

|

|[http://rebase.neb.com/rebase/enz/AquIII.html 20239]

|Agmenellum quadruplicatum PR-6

| 5' GAGGAG
3' CTCCTC

| 5' ---GAGGAGN17NNN {{pad|2em}} --- 3'
3' ---CTCCTCN17N {{pad|2em}} NN--- 5'

|BseRI

AquIV

|

|[http://rebase.neb.com/rebase/enz/AquIV.html 20240]

|Agmenellum quadruplicatum PR-6

| 5' GRGGAAG
3' CYCCTTC

| 5' ---GRGGAAGN16NNN {{pad|2em}} --- 3'
3' ---CYCCTTCN16N {{pad|2em}} NN--- 5'

|None as of 4/9/19

ArsI

|

|[http://rebase.neb.com/rebase/enz/ArsI.html 19405]

|Arthrobacter sp. NTS

| 5' GACN6TTYG
3' CTGN6AARC

| 5' ---NNNNN {{pad|2em}} NN7GACN6TTYGN5NNNNNN {{pad|2em}} --- 3'
3' --- {{pad|2em}} NNNNNNN7CTGN6AARCN5N {{pad|2em}} NNNNN--- 5'

|None as of Oct. 28 2020

AscI{{Cite journal| vauthors =Wei H, Therrien C, Blanchard A, Guan S, Zhu Z | s2cid =10463398 | title =The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases | journal =Nucleic Acids Res | volume = 36| issue = 9| pages = e50|date=May 2008 | pmid = 18413342 | doi =10.1093/nar/gkn182 | pmc=2396408}}{{Cite journal| vauthors = Bilcock DT, Daniels LE, Bath AJ, Halford SE | title = Reactions of type II restriction endonucleases with 8-base pair recognition sites | journal = J Biol Chem | volume = 274| issue = 51| pages = 36379–86 |date=December 1999| pmid = 10593932 | doi = 10.1074/jbc.274.51.36379 | doi-access = free }}

|

|

|Arthrobacter sp.

| 5' GGCGCGCC
3' CCGCGCGG

| 5' ---GG {{pad|2em}} CGCGCC--- 3'
3' ---CCGCGC {{pad|2em}} GG--- 5'

| {{pad|0.1em}}PalAI, SgsI{{pad|0.1em}}

AseI{{Cite journal| vauthors = Polisson C, Morgan RD | title =AseI, a restriction endonuclease from Aquaspirillum serpens which recognizes 5'AT—TAAT3' | journal =Nucleic Acids Res | volume =16 | issue = 21| pages = 10365|date=November 1988| pmid =3264065 | doi = 10.1093/nar/16.21.10365 | pmc=338867}}

|

|[http://rebase.neb.com/rebase/enz/AseI.html 96]

|Aquaspirillum serpens

| 5' ATTAAT
3' TAATTA

| 5' ---AT {{pad|2em}} TAAT--- 3'
3' ---TAAT {{pad|2em}} TA--- 5'

| AsnI, BpoAI, PshBI, Sru4DI, VspI

AseII

|

|[http://rebase.neb.com/rebase/enz/AseII.html 97]

|Aquaspirillum serpens

| 5' CCSGG
3' GGSCC

| 5' ---CC {{pad|2em}} SGG--- 3'
3' ---GGS {{pad|2em}} CC--- 5'

| AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Kpn49kII, Mgl14481I, NciI

AsiI

|

|

|Azotobacter sp.

| 5' GGATCC
3' CCTAGG

| 5' ---G {{pad|2em}} GATCC--- 3'
3' ---CCTAG {{pad|2em}} G--- 5'

| ApaCI, BamHI, BnaI, Bsp4009I, BstI, OkrAI, Pfl8I, SurI, Uba4009I

AsiAI

|

|[http://rebase.neb.com/rebase/enz/AsiAI.html 2853]

|Arthrobacter sp. A7359

| 5' ACCGGT
3' TGGCCA

| 5' ---A {{pad|2em}} CCGGT--- 3'
3' ---TGGCC {{pad|2em}} A--- 5'

| AgeI, AsiGI, BshTI, CsiAI, CspAI, PinAI

AsiGI

|

|[http://rebase.neb.com/rebase/enz/AsiGI.html 10828]

|Arthrobacter sp. G

| 5' ACCGGT
3' TGGCCA

| 5' ---A {{pad|2em}} CCGGT--- 3'
3' ---TGGCC {{pad|2em}} A--- 5'

| AgeI, AsiAI, BshTI, CsiAI, CspAI, PinAI

AsiSI

|

|

|Arthrobacter sp.

| 5' GCGATCGC
3' CGCTAGCG

| 5' ---GCGAT {{pad|2em}} CGC--- 3'
3' ---CGC {{pad|2em}} TAGCG--- 5'

| {{pad|0.1em}}RgaI, SfaAI,{{pad|0.1em}}, SgfI

Asi256I

|

|[http://rebase.neb.com/rebase/enz/Asi256I.html 18472]

|Arthrobacter sp. Ck256

| 5' GATC
3' CTAG

| 5' ---G {{pad|2em}} ATC--- 3'
3' ---CTA {{pad|2em}} G--- 5'

|See [http://rebase.neb.com/cgi-bin/isoget?Asi256I this site] for a complete list.

Asi372I

|

|[http://rebase.neb.com/rebase/enz/Asi372I.html 15803]

|Arthrobacter sp. Mn372

| 5' ATGCAT
3' TACGTA

| 5' ---ATGCA {{pad|2em}} T--- 3'
3' ---T {{pad|2em}} ACGTA--- 5'

|Ppu10I, EcoT22I, Mph1103I, NsiI, Zsp2I, BfrBI, Csp68KIII, PinBI, SepI, SspD5II

AsnI{{Cite journal| vauthors = Rexer BU, Jarsch M, Sagmeister C, Glück B, Berger G, Kessler C | title =AsnI: a novel class II restriction endonuclease from Arthrobacter sp., strain N-CM, recognizing 5'-AT/TAAT-3' | journal = FEBS Lett| volume =235 | issue = 1–2| pages = 241–6|date=August 1988| pmid = 2841156 | doi = 10.1016/0014-5793(88)81271-7| doi-access = }}

|

|[http://rebase.neb.com/rebase/enz/AsnI.html 98]

|Arthrobacter sp. N-CM

| 5' ATTAAT
3' TAATTA

| 5' ---AT {{pad|2em}} TAAT--- 3'
3' ---TAAT {{pad|2em}} TA--- 5'

| AseI, BpoAI, PshBI, Sru4DI, VspI

AspI{{Cite journal| vauthors = Bolton BJ, Schmitz GG, Jarsch M, Kessler C | title = AspI, a novel isoschizomer of Tth111I from Achromobacter species 699 recognizing 5'-GACN/NNGTC-3' | journal = Nucleic Acids Res| volume = 18| issue = 11| pages = 3422|date=June 1990 | pmid = 2162524 | doi = 10.1093/nar/18.11.3422 | pmc=330974}}

|

|

|Achromobacter sp. 699

| 5' GACNNNGTC
3' CTGNNNCAG

| 5' ---GACN {{pad|2em}} NNGTC--- 3'
3' ---CTGNN {{pad|2em}} NCAG--- 5'

| AtsI, PflFI, PsyI, TelI, Tth111I

Asp10HI

|

|

|Acidiphilium sp. 10H

| 5' TTCGAA
3' AAGCTT

| 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5'

| AsuII, BspT104I, BstBI, CbiI, Csp68KII, PlaII, PpaAI, Ssp1I

Asp10HII

|

|[http://rebase.neb.com/rebase/enz/Asp10HII.html 100]

|Acidiphilium sp. 10H

| 5' CCAN5TGG
3' GGTN5ACC

| 5' ---CCANNNN {{pad|2em}} NTGG--- 3'
3' ---GGTN {{pad|2em}} NNNNACC--- 5'

| AccB7I, AcpII, BasI, Esp1396I, PflBI, PflMI, Van91I

Asp26HI

|

|[http://rebase.neb.com/rebase/enz/Asp26HI.html 111]

|Acidiphilium sp. 26H

| 5' GAATGC
3' CTTACG

| 5' ---GAATGCN {{pad|2em}} --- 3'
3' ---CTTAC {{pad|2em}} GN--- 5'

| Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, PctI

Asp27HI

|

|[http://rebase.neb.com/rebase/enz/Asp27HI.html 112]

|Acidiphilium sp. 27H

| 5' GAATGC
3' CTTACG

| 5' ---GAATGCN {{pad|2em}} --- 3'
3' ---CTTAC {{pad|2em}} GN--- 5'

| Asp35HI, Asp36HI, Asp40HI, {{pad|0.1em}}BmaHI,{{pad|0.1em}} BsaMI, BscCI, BsmI

Asp35HI

|

|[http://rebase.neb.com/rebase/enz/Asp35HI.html 117]

|Acidiphilium sp. 35H

| 5' GAATGC
3' CTTACG

| 5' ---GAATGCN {{pad|2em}} --- 3'
3' ---CTTAC {{pad|2em}} GN--- 5'

| Asp36HI, Asp40HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I

Asp36HI

|

|[http://rebase.neb.com/rebase/enz/Asp36HI.html 118]

|Acidiphilium sp. 36H

| 5' GAATGC
3' CTTACG

| 5' ---GAATGCN {{pad|2em}} --- 3'
3' ---CTTAC {{pad|2em}} GN--- 5'

| Asp40HI, Asp50HI, {{pad|0.1em}}BmaHI,{{pad|0.1em}} BscCI, BsmI, Mva1269I, PctI

Asp40HI

|

|[http://rebase.neb.com/rebase/enz/Asp40HI.html 120]

|Acidiphilium sp. 40H

| 5' GAATGC
3' CTTACG

| 5' ---GAATGCN {{pad|2em}} --- 3'
3' ---CTTAC {{pad|2em}} GN--- 5'

| Asp27HI, Asp36HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I

Asp50HI

|

|[http://rebase.neb.com/rebase/enz/Asp50HI.html 122]

|Acidiphilium sp. 50H

| 5' GAATGC
3' CTTACG

| 5' ---GAATGCN {{pad|2em}} --- 3'
3' ---CTTAC {{pad|2em}} GN--- 5'

| Asp26HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, Mva1269I

Asp700I{{Cite journal| vauthors =Bolton BJ, Comer M, Kessler C | title =Asp700I, a novel isoschizomer of XmnI from Achromobacter species 700 recognizing 5'-GAANN/NNTTC-3' | journal =Nucleic Acids Res | volume =17 | issue = 21| pages = 8879|date=November 1989 | pmid = 2587236 | doi =10.1093/nar/17.21.8879 | pmc=335071}}

|

|

|Achromobacter sp. 700

| 5' GAAN4TTC
3' CTTN4AAG

| 5' ---GAANN {{pad|2em}} NNTTC--- 3'
3' ---CTTNN {{pad|2em}} NNAAG--- 5'

| BbvAI, MroXI, PdmI, XmnI

Asp713I

|

|[http://rebase.neb.com/rebase/enz/Asp713I.html 131]

|Achromobacter sp. 713

| 5' CTGCAG
3' GACGTC

| 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5'

| AjoI, AliAJI, BloHII, CfrA4I, Ecl37kI, MhaAI, PstI, SflI, Srl5DI, YenI

Asp718I{{Cite journal| vauthors = Mural RJ | title = Cleavage by the restriction endonuclease Asp718, an isoschizomer of KpnI, is sensitive to Escherichia coli Dcm methylation | journal = Nucleic Acids Res | volume = 15| issue = 21| pages = 9085|date=November 1987 | pmid = 2825125 | doi = 10.1093/nar/15.21.9085 | pmc=306431}}

|

|

|Achromobacter sp. 718

| 5' GGTACC
3' CCATGG

| 5' ---G {{pad|2em}} GTACC--- 3'
3' ---CCATG {{pad|2em}} G--- 5'

| Acc65I, AhaB8I, KpnI, SthI

Asp745I

|

|

|Achromobacter sp. 745

| 5' GGWCC
3' CCWGG

| 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5'

| Bme18I, BsrAI, CauI, Eco47I, ErpI, FdiI, SinI, VpaK11AI, VpaK11BI

AspAI

|

|

|Alcaligenes sp.

| 5' GGTNACC
3' CCANTGG

| 5' ---G {{pad|2em}} GTNACC--- 3'
3' ---CCANTG {{pad|2em}} G--- 5'

| AcrII, Bse64I, BseT10I, BstEII, BstPI, Eco91I, EcoO65I, PspEI

AspA2I

|

|[http://rebase.neb.com/rebase/enz/AspA2I.html 6959]

|Arthrobacter sp. A2

| 5' CCTAGG
3' GGATCC

| 5' ---C {{pad|2em}} CTAGG--- 3'
3' ---GGATC {{pad|2em}} C--- 5'

| AvrBII, AvrII, BlnI, BspA2I, XmaJI

AspEI{{Cite journal| vauthors = Frey B, Kaluza K, Auer J, Stratidakis I, Rina M, Bouriotis V, Schmitz G | title = AspEI, a novel Eam11051 isoschizomer from Aureobacterium species recognizing 5'-GACnnn/nnGTC-3' | journal = Nucleic Acids Res | volume =20| issue =14 | pages = 3782|date=July 1992 | pmid = 1641345 | doi = 10.1093/nar/20.14.3782 | pmc=334034}}

|

|

|Aureobacterium sp.

| 5' GACN5GTC
3' CTGN5CAG

| 5' ---GACNNN {{pad|2em}} NNGTC--- 3'
3' ---CTGNN {{pad|2em}} NNNCAG--- 5'

| AhdI, {{pad|0.1em}}BmeRI,{{pad|0.1em}} BspOVI, DriI, Eam1105I, EclHKI, NruGI

AspHI{{Cite journal| vauthors = Bolton BJ, Höltke HJ, Schmitz GG, Jarsch M, Kessler C | title = AspHI, a novel isoschizomer of HgiAI from Achromobacter species H recognizing 5'-GWGCW/C-3' | journal = Nucleic Acids Res | volume = 17| issue =22 | pages = 9500|date=November 1989 | pmid = 2587281 | doi = 10.1093/nar/17.22.9500 | pmc=335177}}

|

|

|Achromobacter sp. H

| 5' GWGCWC
3' CWCGWG

| 5' ---GWGCW {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} WCGWG--- 5'

| Alw21I, Bbv12I, Bsh45I, BsiHKAI, {{pad|0.1em}}Bsm6I,{{pad|0.1em}} HgiAI, MspV281I

AspLEI

|

|

|Arthrobacter sp. LE3860

| 5' GCGC
3' CGCG

| 5' ---GCG {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} GCG--- 5'

| BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI

AspMI

|

|[http://rebase.neb.com/rebase/enz/AspMI.html 2977]

|Acinetobacter sp. M

| 5' AGGCCT
3' TCCGGA

| 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5'

| AatI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI

AspMDI

|

|

|Alcaligenes sp. MD1

| 5' GATC
3' CTAG

| 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5'

| BfuCI, Bsp143I, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI

AspNI

|

|

|Anabaena sp. J3

| 5' GGNNCC
3' CCNNGG

| 5' ---GGN {{pad|2em}} NCC--- 3'
3' ---CCN {{pad|2em}} NGG--- 5'

| {{pad|0.1em}}BmiI,{{pad|0.1em}} BscBI, BspLI, NlaIV, PspN4I

AspS9I

|

|

|Arthrobacter sp. S9

| 5' GGNCC
3' CCNGG

| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'

| AsuI, AvcI, Bac36I, Bal228I, FmuI, NspIV, PspPI, Sau96I

AssI

|

|[http://rebase.neb.com/rebase/enz/AssI.html 4930]

|Arthrobacter sp.

| 5' AGTACT
3' TCATGA

| 5' ---AGT {{pad|2em}} ACT--- 3'
3' ---TCA {{pad|2em}} TGA--- 5'

| Acc113I, {{pad|0.1em}}BmcAI, Bpa34I,{{pad|0.1em}} DpaI, Eco255I, RflFII, ScaI, ZrmI

AstWI

|

|

|Anabaena sp.

| 5' GRCGYC
3' CYGCRG

| 5' ---GR {{pad|2em}} CGYC--- 3'
3' ---CYGC {{pad|2em}} RG--- 5'

| AcyI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiGI, Msp17I

AsuI{{Cite journal| vauthors =Hughes SG, Bruce T, Murray K | title =The Isolation and Characterization of a Sequence-Specific Endonuclease from Anabaena subcylindrica | journal =Biochem J | volume =185 | issue = 1| pages = 59–63|date=January 1980| pmid = 6246880 | pmc=1161269| doi =10.1042/bj1850059 }}

|

|

|Anabaena subcylindrica

| 5' GGNCC
3' CCNGG

| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'

| AspS9I, AvcI, CcuI, Cfr13I, FmuI, MaeK81II, Nsp7121I, PspPI, UnbI

AsuII{{Cite journal| author = Roberts RJ| title =Restriction and modification enzymes and their recognition sequences | journal = Gene| volume = 8 | issue = 4| pages = 329–43|date=March 1980| pmid = 6245015 | doi = 10.1016/0378-1119(80)90040-2| pmc = 320508}}

|

|

|Anabaena subcylindrica

| 5' TTCGAA
3' AAGCTT

| 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5'

| AcpI, Bpu14I, Bsp119I, BspT104I, Csp45I, FspII, LspI, NspV, SfuI

AsuIII

|

|

|Anabaena subcylindrica

| 5' GRCGYC
3' CYGCRG

| 5' ---GR {{pad|2em}} CGYC--- 3'
3' ---CYGC {{pad|2em}} RG--- 5'

| AcyI, AsuIII, BbiII, BsaHI, BstACI, Hin1I, HgiHII, Hsp92I, Msp17I

AsuC2I

|

|[http://rebase.neb.com/rebase/enz/AsuC2I.html 2901]

|Actinobacillus suis C2

| 5' CCSGG
3' GGSCC

| 5' ---CC {{pad|2em}} SGG--- 3'
3' ---GGS {{pad|2em}} CC--- 5'

| AhaI, AseII, BcnI, Eco1831I, EcoHI, Kpn49kII, Mgl14481I, NciI

AsuHPI{{Cite journal| vauthors = Dedkov VS, Degtyarev SK| title = Actinobacillus and Streptococcus: producers of isoschizomers of the restriction endonucleases R.HphI, R.SauI, R.NheI, R.MboI and R.SwaI | journal = Biol Chem | volume = 379| issue = 4–5| pages =573–4 |date=April–May 1998 | pmid = 9628357 }}

|

|[http://rebase.neb.com/rebase/enz/AsuHPI.html 2811]

|Actinobacillus suis HP

| 5' GGTGA
3' CCACT

| 5' ---GGTGAN6NN {{pad|2em}} --- 3'
3' ---CCACTN6N {{pad|2em}} N--- 5'

| HphI, SspD5I

AsuNHI

|

|

|Actinobacillus suis NH

| 5' GCTAGC
3' CGATCG

| 5' ---G {{pad|2em}} CTAGC--- 3'
3' ---CGATC {{pad|2em}} G--- 5'

| AceII, BmtI, {{pad|0.1em}}BspOI,{{pad|0.1em}} LlaG2I, NheI, PstNHI

AtsI

|

|

|Aureobacterium testaceum 4842

| 5' GACNNNGTC
3' CTGNNNCAG

| 5' ---GACN {{pad|2em}} NNGTC--- 3'
3' ---CTGNN {{pad|2em}} NCAG--- 5'

| AspI, PflFI, PsyI, TelI, Tth111I

AvaI{{Cite journal| vauthors =Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A, Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M, Takazawa M, Yamada M, Yasuda M, Tabata S | title =Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 | journal =DNA Res| volume = 8| issue = 5| pages = 227–53|date=October 2001| pmid = 11759840 | doi = 10.1093/dnares/8.5.205| doi-access = free}}{{Cite journal| vauthors =Hughes SG, Murray K | title = The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis | journal = Biochem J| volume = 185| issue = 1| pages = 65–75|date=January 1980| pmid = 6246881 | pmc=1161270| doi = 10.1042/bj1850065 }}

|

|

|Anabaena variabilis

| 5' CYCGRG
3' GRGCYC

| 5' ---C {{pad|2em}} YCGRG--- 3'
3' ---GRGCY {{pad|2em}} C--- 5'

| AquI, Ama87I, BsiHKCI, BsoBI, BspLU4I, Eco88I, NspIII, PlaAI

AvaII

|

|

|Anabaena variabilis

| 5' GGWCC
3' CCWGG

| 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5'

| Bme18I, Bme216I, BsrAI, Eco47I, Kzo49I, Psp03I, SmuEI, VpaK11AI

AvcI

|

|

|Actinomyces violaceoniger cristalomycini

| 5' GGNCC
3' CCNGG

| 5' ---G {{pad|2em}} GNCC--- 3'
3' ---CCNG {{pad|2em}} G--- 5'

| AspS9I, BshKI, BsiZI, Bsp1894I, BspBII, BspF4I, Bsu54I, Pde12I

AviII

|

|

|Anabaena variabillis (halle)

| 5' TGCGCA
3' ACGCGT

| 5' ---TGC {{pad|2em}} GCA--- 3'
3' ---ACG {{pad|2em}} CGT--- 5'

| Acc16I, AosI, FdiII, FspI, MstI, NsbI, PamI, Pun14627I

AvrII

|

|[http://rebase.neb.com/rebase/enz/AvrII.html 173]

|Anabaena variabilis UW

| 5' CCTAGG
3' GGATCC

| 5' ---C {{pad|2em}} CTAGG--- 3'
3' ---GGATC {{pad|2em}} C--- 5'

| AspA2I, AvrBII, BlnI, BspA2I, XmaJI

AvrBII

|

|[http://rebase.neb.com/rebase/enz/AvrBII.html 171]

|Arthrobacter variabilis

| 5' CCTAGG
3' GGATCC

| 5' ---C {{pad|2em}} CTAGG--- 3'
3' ---GGATC {{pad|2em}} C--- 5'

| AspA2I, AvrII, BlnI, BspA2I, XmaJI

AxyI{{Cite journal| vauthors = Yoshioka H, Nakamura H, Sasaki J, Tahara Y, Yamada Y| title = Purification, properties and recognition sequence of site-specific restriction endonuclease from "Acetobacter xylinus"| journal =Agric Biol Chem | volume = 47| issue = 12| pages = 2871–9| year = 1983 | doi = 10.1271/bbb1961.47.2871}}

|

|[http://rebase.neb.com/rebase/enz/AxyI.html 174]

|Acetobacter xylinus

| 5' CCTNAGG
3' GGANTCC

| 5' ---CC {{pad|2em}} TNAGG--- 3'
3' ---GGANT {{pad|2em}} CC--- 5'

| AocI, BliHKI, Bse21I, BspR7I, Bsu36I, CvnI, Eco81I, Lmu60I

§ An HF version of this enzyme is available

:{{Incomplete list|date=September 2010}}

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Notes

{{Reflist|colwidth=50em}}

{{restriction enzyme}}

{{DEFAULTSORT:Restriction enzyme cutting sites: A}}

Category:Biotechnology

Restriction enzyme cutting sites

Category:Restriction enzymes