MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.{{cite web | title = Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4172}}

MCM3 has been shown to interact with:

• CDC45-related protein,
• CDC6,{{cite journal | vauthors = Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T | title = Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation | journal = J. Biol. Chem. | volume = 274 | issue = 36 | pages = 25927–32 | date = September 1999 | pmid = 10464337 | doi = 10.1074/jbc.274.36.25927 | doi-access = free }}{{cite journal | vauthors = Méndez J, Stillman B | title = Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis | journal = Mol. Cell. Biol. | volume = 20 | issue = 22 | pages = 8602–12 | date = November 2000 | pmid = 11046155 | pmc = 102165 | doi = 10.1128/mcb.20.22.8602-8612.2000 }}
• DBF4,
• MCM2
• MCM3AP,{{cite journal | vauthors = Takei Y, Assenberg M, Tsujimoto G, Laskey R | title = The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3 | journal = J. Biol. Chem. | volume = 277 | issue = 45 | pages = 43121–5 | date = November 2002 | pmid = 12226073 | doi = 10.1074/jbc.C200442200 | doi-access = free }}{{cite journal | vauthors = Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N | title = A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication | journal = Blood | volume = 95 | issue = 7 | pages = 2321–8 | date = April 2000 | pmid = 10733502 | doi = 10.1182/blood.V95.7.2321}}{{cite journal | vauthors = Takei Y, Tsujimoto G | title = Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization | journal = J. Biol. Chem. | volume = 273 | issue = 35 | pages = 22177–80 | date = August 1998 | pmid = 9712829 | doi = 10.1074/jbc.273.35.22177 | doi-access = free }}
• MCM5,{{cite journal | vauthors = DaFonseca CJ, Shu F, Zhang JJ | title = Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 98 | issue = 6 | pages = 3034–9 | date = March 2001 | pmid = 11248027 | pmc = 30602 | doi = 10.1073/pnas.061487598 | bibcode = 2001PNAS...98.3034D | doi-access = free }}
• MCM7,{{cite journal | vauthors = Fujita M, Kiyono T, Hayashi Y, Ishibashi M | title = In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP | journal = J. Biol. Chem. | volume = 272 | issue = 16 | pages = 10928–35 | date = April 1997 | pmid = 9099751 | doi = 10.1074/jbc.272.16.10928 | doi-access = free }}{{cite journal | vauthors = Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T | title = Nuclear organization of DNA replication initiation proteins in mammalian cells | journal = J. Biol. Chem. | volume = 277 | issue = 12 | pages = 10354–61 | date = March 2002 | pmid = 11779870 | doi = 10.1074/jbc.M111398200 | doi-access = free }}
• ORC4L,{{cite journal | vauthors = Kneissl M, Pütter V, Szalay AA, Grummt F | title = Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells | journal = J. Mol. Biol. | volume = 327 | issue = 1 | pages = 111–28 | date = March 2003 | pmid = 12614612 | doi = 10.1016/s0022-2836(03)00079-2 }} and
• ORC5L.

• Mini Chromosome Maintenance

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• {{cite journal | vauthors = Musahl C, Schulte D, Burkhart R, Knippers R | title = A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins | journal = Eur. J. Biochem. | volume = 230 | issue = 3 | pages = 1096–101 | year = 1995 | pmid = 7601140 | doi = 10.1111/j.1432-1033.1995.tb20660.x }}
• {{cite journal | vauthors = Schulte D, Burkhart R, Musahl C, Hu B, Schlatterer C, Hameister H, Knippers R | title = Expression, phosphorylation and nuclear localization of the human P1 protein, a homologue of the yeast Mcm 3 replication protein | journal = J. Cell Sci. | volume = 108 | issue = 4 | pages = 1381–9 | year = 1995 | doi = 10.1242/jcs.108.4.1381 | pmid = 7615659 }}
• {{cite journal | vauthors = Starborg M, Brundell E, Gell K, Larsson C, White I, Daneholt B, Höög C | title = A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication | journal = J. Cell Sci. | volume = 108 | issue = 3 | pages = 927–34 | year = 1995 | doi = 10.1242/jcs.108.3.927 | pmid = 7622621 }}
• {{cite journal | vauthors = Burkhart R, Schulte D, Hu D, Musahl C, Göhring F, Knippers R | title = Interactions of human nuclear proteins P1Mcm3 and P1Cdc46 | journal = Eur. J. Biochem. | volume = 228 | issue = 2 | pages = 431–8 | year = 1995 | pmid = 7705359 | doi = 10.1111/j.1432-1033.1995.tb20281.x }}
• {{cite journal | vauthors = Kubota Y, Mimura S, Nishimoto S, Takisawa H, Nojima H | title = Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor | journal = Cell | volume = 81 | issue = 4 | pages = 601–9 | year = 1995 | pmid = 7758114 | doi = 10.1016/0092-8674(95)90081-0 | s2cid = 18797719 | doi-access = free }}
• {{cite journal | vauthors = Madine MA, Khoo CY, Mills AD, Laskey RA | title = MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells | journal = Nature | volume = 375 | issue = 6530 | pages = 421–4 | year = 1995 | pmid = 7760938 | doi = 10.1038/375421a0 | bibcode = 1995Natur.375..421M | s2cid = 4316044 }}
• {{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1–2 | pages = 171–4 | year = 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}
• {{cite journal | vauthors = Hu B, Burkhart R, Schulte D, Musahl C, Knippers R | title = The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins | journal = Nucleic Acids Res. | volume = 21 | issue = 23 | pages = 5289–93 | year = 1994 | pmid = 8265339 | pmc = 310560 | doi = 10.1093/nar/21.23.5289-a }}
• {{cite journal | vauthors = Schulte D, Richter A, Burkhart R, Musahl C, Knippers R | title = Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins | journal = Eur. J. Biochem. | volume = 235 | issue = 1–2 | pages = 144–51 | year = 1996 | pmid = 8631321 | doi = 10.1111/j.1432-1033.1996.00144.x | doi-access = free }}
• {{cite journal | vauthors = Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H | title = Binding of human minichromosome maintenance proteins with histone H3 | journal = J. Biol. Chem. | volume = 271 | issue = 39 | pages = 24115–22 | year = 1996 | pmid = 8798650 | doi = 10.1074/jbc.271.39.24115 | doi-access = free }}
• {{cite journal | vauthors = Sato N, Arai K, Masai H | title = Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7 | journal = EMBO J. | volume = 16 | issue = 14 | pages = 4340–51 | year = 1997 | pmid = 9250678 | pmc = 1170060 | doi = 10.1093/emboj/16.14.4340 }}
• {{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1–2 | pages = 149–56 | year = 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}
• {{cite journal | vauthors = Takei Y, Tsujimoto G | title = Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization | journal = J. Biol. Chem. | volume = 273 | issue = 35 | pages = 22177–80 | year = 1998 | pmid = 9712829 | doi = 10.1074/jbc.273.35.22177 | doi-access = free }}
• {{cite journal | vauthors = Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R, Darnell JE | title = Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation | journal = EMBO J. | volume = 17 | issue = 23 | pages = 6963–71 | year = 1999 | pmid = 9843502 | pmc = 1171044 | doi = 10.1093/emboj/17.23.6963 }}
• {{cite journal | vauthors = Yankulov K, Todorov I, Romanowski P, Licatalosi D, Cilli K, McCracken S, Laskey R, Bentley DL | title = MCM Proteins Are Associated with RNA Polymerase II Holoenzyme | journal = Mol. Cell. Biol. | volume = 19 | issue = 9 | pages = 6154–63 | year = 1999 | pmid = 10454562 | pmc = 84545 | doi = 10.1128/MCB.19.9.6154}}
• {{cite journal | vauthors = Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T | title = Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation | journal = J. Biol. Chem. | volume = 274 | issue = 36 | pages = 25927–32 | year = 1999 | pmid = 10464337 | doi = 10.1074/jbc.274.36.25927 | doi-access = free }}
• {{cite journal | vauthors = Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N | title = A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication | journal = Blood | volume = 95 | issue = 7 | pages = 2321–8 | year = 2000 | pmid = 10733502 | doi = 10.1182/blood.V95.7.2321}}
• {{cite journal | vauthors = Hofmann Y, Becker J, Wright F, Avner ED, Mrug M, Guay-Woodford LM, Somlo S, Zerres K, Germino GG, Onuchic LF | title = Genomic structure of the gene for the human P1 protein (MCM3) and its exclusion as a candidate for autosomal recessive polycystic kidney disease | journal = Eur. J. Hum. Genet. | volume = 8 | issue = 3 | pages = 163–6 | year = 2000 | pmid = 10780780 | doi = 10.1038/sj.ejhg.5200426 | doi-access = free }}
• {{cite journal | vauthors = Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y, Takahashi EI, Sakaguchi N | title = Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication | journal = Gene | volume = 255 | issue = 2 | pages = 219–27 | year = 2000 | pmid = 11024281 | doi = 10.1016/S0378-1119(00)00336-X }}

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