MCM7

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.{{cite web | title = Entrez Gene: MCM7 MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4176}}

MCM7 has been shown to interact with:

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• CDC45-related protein{{cite journal | vauthors = Kukimoto I, Igaki H, Kanda T | title = Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha | journal = Eur. J. Biochem. | volume = 265 | issue = 3 | pages = 936–43 | date = November 1999 | pmid = 10518787 | doi = 10.1046/j.1432-1327.1999.00791.x| doi-access = free }}
• CDC6,
• Cell division cycle 7-related protein kinase,
• DBF4,
• MCM2,{{cite journal | vauthors = You Z, Komamura Y, Ishimi Y | title = Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity | journal = Mol. Cell. Biol. | volume = 19 | issue = 12 | pages = 8003–15 | date = December 1999 | pmid = 10567526 | pmc = 84885 | doi = 10.1128/MCB.19.12.8003}}
• MCM3,{{cite journal | vauthors = Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T | title = Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation | journal = J. Biol. Chem. | volume = 274 | issue = 36 | pages = 25927–32 | date = September 1999 | pmid = 10464337 | doi = 10.1074/jbc.274.36.25927| doi-access = free }}{{cite journal | vauthors = Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T | title = Nuclear organization of DNA replication initiation proteins in mammalian cells | journal = J. Biol. Chem. | volume = 277 | issue = 12 | pages = 10354–61 | date = March 2002 | pmid = 11779870 | doi = 10.1074/jbc.M111398200 | doi-access = free }}
• MCM4,{{cite journal | vauthors = Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H | title = Binding of human minichromosome maintenance proteins with histone H3 | journal = J. Biol. Chem. | volume = 271 | issue = 39 | pages = 24115–22 | date = September 1996 | pmid = 8798650 | doi = 10.1074/jbc.271.39.24115| doi-access = free }}
• MCM5,{{cite journal | vauthors = Fujita M, Kiyono T, Hayashi Y, Ishibashi M | title = In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP | journal = J. Biol. Chem. | volume = 272 | issue = 16 | pages = 10928–35 | date = April 1997 | pmid = 9099751 | doi = 10.1074/jbc.272.16.10928| doi-access = free }}{{cite journal | vauthors = DaFonseca CJ, Shu F, Zhang JJ | title = Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 98 | issue = 6 | pages = 3034–9 | date = March 2001 | pmid = 11248027 | pmc = 30602 | doi = 10.1073/pnas.061487598 | bibcode = 2001PNAS...98.3034D | doi-access = free }}
• MCM6,{{cite journal | vauthors = Yabuta N, Kajimura N, Mayanagi K, Sato M, Gotow T, Uchiyama Y, Ishimi Y, Nojima H | title = Mammalian Mcm2/4/6/7 complex forms a toroidal structure | journal = Genes Cells | volume = 8 | issue = 5 | pages = 413–21 | date = May 2003 | pmid = 12694531 | doi = 10.1046/j.1365-2443.2003.00645.x| s2cid = 27707848 | doi-access = free }}{{cite journal | vauthors = You Z, Ishimi Y, Masai H, Hanaoka F | title = Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex | journal = J. Biol. Chem. | volume = 277 | issue = 45 | pages = 42471–9 | date = November 2002 | pmid = 12207017 | doi = 10.1074/jbc.M205769200 | doi-access = free }}
• MNAT1,{{cite journal | vauthors = Wang Y, Xu F, Hall FL | title = The MAT1 cyclin-dependent kinase-activating kinase (CAK) assembly/targeting factor interacts physically with the MCM7 DNA licensing factor | journal = FEBS Lett. | volume = 484 | issue = 1 | pages = 17–21 | date = October 2000 | pmid = 11056214 | doi = 10.1016/s0014-5793(00)02117-7| s2cid = 44625458 | doi-access = }}
• ORC1L,
• ORC2L,
• ORC3L,
• ORC5L,
• Replication protein A1,{{cite journal | vauthors = Kneissl M, Pütter V, Szalay AA, Grummt F | title = Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells | journal = J. Mol. Biol. | volume = 327 | issue = 1 | pages = 111–28 | date = March 2003 | pmid = 12614612 | doi = 10.1016/s0022-2836(03)00079-2}}
• Retinoblastoma protein,{{cite journal | vauthors = Sterner JM, Dew-Knight S, Musahl C, Kornbluth S, Horowitz JM | title = Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7 | journal = Mol. Cell. Biol. | volume = 18 | issue = 5 | pages = 2748–57 | date = May 1998 | pmid = 9566894 | pmc = 110654 | doi = 10.1128/mcb.18.5.2748}} and
• UBE3A.{{cite journal | vauthors = Kühne C, Banks L | title = E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box | journal = J. Biol. Chem. | volume = 273 | issue = 51 | pages = 34302–9 | date = December 1998 | pmid = 9852095 | doi = 10.1074/jbc.273.51.34302| doi-access = free }}

{{Div col end}}

• Mini Chromosome Maintenance

{{reflist}}

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• {{cite journal | vauthors = Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V | title = Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle. | journal = Biochem. Biophys. Res. Commun. | volume = 177 | issue = 1 | pages = 395–400 | year = 1991 | pmid = 1710453 | doi = 10.1016/0006-291X(91)91996-P }}
• {{cite journal | vauthors = Musahl C, Schulte D, Burkhart R, Knippers R | title = A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins. | journal = Eur. J. Biochem. | volume = 230 | issue = 3 | pages = 1096–101 | year = 1995 | pmid = 7601140 | doi = 10.1111/j.1432-1033.1995.tb20660.x }}
• {{cite journal | vauthors = Mincheva A, Todorov I, Werner D, Fink TM, Lichter P | title = The human gene for nuclear protein BM28 (CDCL1), a new member of the early S-phase family of proteins, maps to chromosome band 3q21. | journal = Cytogenet. Cell Genet. | volume = 65 | issue = 4 | pages = 276–7 | year = 1994 | pmid = 8258304 | doi = 10.1159/000133647 }}
• {{cite journal | vauthors = Hu B, Burkhart R, Schulte D, Musahl C, Knippers R | title = The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins. | journal = Nucleic Acids Res. | volume = 21 | issue = 23 | pages = 5289–93 | year = 1994 | pmid = 8265339 | pmc = 310560 | doi = 10.1093/nar/21.23.5289-a }}
• {{cite journal | vauthors = Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA | title = A "double adaptor" method for improved shotgun library construction. | journal = Anal. Biochem. | volume = 236 | issue = 1 | pages = 107–13 | year = 1996 | pmid = 8619474 | doi = 10.1006/abio.1996.0138 }}
• {{cite journal | vauthors = Fujita M, Kiyono T, Hayashi Y, Ishibashi M | title = hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase. | journal = J. Biol. Chem. | volume = 271 | issue = 8 | pages = 4349–54 | year = 1996 | pmid = 8626784 | doi = 10.1074/jbc.271.8.4349 | doi-access = free }}
• {{cite journal | vauthors = Schulte D, Richter A, Burkhart R, Musahl C, Knippers R | title = Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins. | journal = Eur. J. Biochem. | volume = 235 | issue = 1–2 | pages = 144–51 | year = 1996 | pmid = 8631321 | doi = 10.1111/j.1432-1033.1996.00144.x | doi-access = free }}
• {{cite journal | vauthors = Kiyono T, Fujita M, Hayashi Y, Ishibashi M | title = Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family. | journal = Biochim. Biophys. Acta | volume = 1307 | issue = 1 | pages = 31–4 | year = 1996 | pmid = 8652665 | doi = 10.1016/0167-4781(96)00057-7 }}
• {{cite journal | vauthors = Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H | title = Binding of human minichromosome maintenance proteins with histone H3. | journal = J. Biol. Chem. | volume = 271 | issue = 39 | pages = 24115–22 | year = 1996 | pmid = 8798650 | doi = 10.1074/jbc.271.39.24115 | doi-access = free }}
• {{cite journal | vauthors = Fujita M, Kiyono T, Hayashi Y, Ishibashi M | title = In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP. | journal = J. Biol. Chem. | volume = 272 | issue = 16 | pages = 10928–35 | year = 1997 | pmid = 9099751 | doi = 10.1074/jbc.272.16.10928 | doi-access = free }}
• {{cite journal | vauthors = Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA | title = Large-scale concatenation cDNA sequencing. | journal = Genome Res. | volume = 7 | issue = 4 | pages = 353–8 | year = 1997 | pmid = 9110174 | pmc = 139146 | doi = 10.1101/gr.7.4.353 }}
• {{cite journal | vauthors = Hiraiwa A, Fujita M, Nagasaka T, Adachi A, Ohashi M, Ishibashi M | title = Immunolocalization of hCDC47 protein in normal and neoplastic human tissues and its relation to growth. | journal = Int. J. Cancer | volume = 74 | issue = 2 | pages = 180–4 | year = 1997 | pmid = 9133452 | doi = 10.1002/(SICI)1097-0215(19970422)74:2<180::AID-IJC7>3.0.CO;2-V | doi-access = free }}
• {{cite journal | vauthors = Ishimi Y | title = A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. | journal = J. Biol. Chem. | volume = 272 | issue = 39 | pages = 24508–13 | year = 1997 | pmid = 9305914 | doi = 10.1074/jbc.272.39.24508 | doi-access = free }}
• {{cite journal | vauthors = Sterner JM, Dew-Knight S, Musahl C, Kornbluth S, Horowitz JM | title = Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. | journal = Mol. Cell. Biol. | volume = 18 | issue = 5 | pages = 2748–57 | year = 1998 | pmid = 9566894 | pmc = 110654 | doi = 10.1128/mcb.18.5.2748}}
• {{cite journal | vauthors = Suzuki S, Adachi A, Hiraiwa A, Ohashi M, Ishibashi M, Kiyono T | title = Cloning and characterization of human MCM7 promoter. | journal = Gene | volume = 216 | issue = 1 | pages = 85–91 | year = 1998 | pmid = 9714754 | doi = 10.1016/S0378-1119(98)00323-0 }}
• {{cite journal | vauthors = Kühne C, Banks L | title = E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box. | journal = J. Biol. Chem. | volume = 273 | issue = 51 | pages = 34302–9 | year = 1999 | pmid = 9852095 | doi = 10.1074/jbc.273.51.34302 | doi-access = free }}
• {{cite journal | vauthors = Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T | title = Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation. | journal = J. Biol. Chem. | volume = 274 | issue = 36 | pages = 25927–32 | year = 1999 | pmid = 10464337 | doi = 10.1074/jbc.274.36.25927 | doi-access = free }}
• {{cite journal | vauthors = Freeman A, Morris LS, Mills AD, Stoeber K, Laskey RA, Williams GH, Coleman N | title = Minichromosome maintenance proteins as biological markers of dysplasia and malignancy. | journal = Clin. Cancer Res. | volume = 5 | issue = 8 | pages = 2121–32 | year = 1999 | pmid = 10473096 }}
• {{cite journal | vauthors = Kukimoto I, Igaki H, Kanda T | title = Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha. | journal = Eur. J. Biochem. | volume = 265 | issue = 3 | pages = 936–43 | year = 1999 | pmid = 10518787 | doi = 10.1046/j.1432-1327.1999.00791.x | doi-access = free }}

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