PIKFYVE

The principal enzymatic activity of PIKfyve is to phosphorylate PtdIns3P to PtdIns(3,5)P2. PIKfyve activity is responsible for the production of both PtdIns(3,5)P2 and phosphatidylinositol 5-phosphate (PtdIns5P).{{cite journal | vauthors = Shisheva A | title = PIKfyve: the road to PtdIns 5-P and PtdIns 3,5-P(2) | journal = Cell Biology International | volume = 25 | issue = 12 | pages = 1201–6 | year = 2001 | pmid = 11748912 | doi = 10.1006/cbir.2001.0803 | s2cid = 29411107 }}{{cite journal | vauthors = Sbrissa D, Ikonomov OC, Filios C, Delvecchio K, Shisheva A | title = Functional dissociation between PIKfyve-synthesized PtdIns5P and PtdIns(3,5)P2 by means of the PIKfyve inhibitor YM201636 | journal = American Journal of Physiology. Cell Physiology | volume = 303 | issue = 4 | pages = C436-46 | date = August 2012 | pmid = 22621786 | pmc = 3422984 | doi = 10.1152/ajpcell.00105.2012 }}{{cite journal | vauthors = Zolov SN, Bridges D, Zhang Y, Lee WW, Riehle E, Verma R, Lenk GM, Converso-Baran K, Weide T, Albin RL, Saltiel AR, Meisler MH, Russell MW, Weisman LS | display-authors = 6 | title = In vivo, Pikfyve generates PI(3,5)P2, which serves as both a signaling lipid and the major precursor for PI5P | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 109 | issue = 43 | pages = 17472–7 | date = October 2012 | pmid = 23047693 | pmc = 3491506 | doi = 10.1073/pnas.1203106109 | bibcode = 2012PNAS..10917472Z | doi-access = free }} PIKfyve is a large protein, containing a number of functional domains and expressed in several spliced forms. The reported full-length mouse and human cDNA clones encode proteins of 2052 and 2098 amino acid residues, respectively. By directly binding membrane PtdIns(3)P, the FYVE finger domain of PIKfyve is essential in localizing the protein to the cytosolic leaflet of endosomes. Impaired PIKfyve enzymatic activity by dominant-interfering mutants, siRNA- mediated ablation or pharmacological inhibition causes lysosome enlargement and cytoplasmic vacuolation due to impaired PtdIns(3,5)P2 synthesis and impaired lysosome fission process and homeostasis.{{cite journal | vauthors = Sharma G, Guardia CM, Roy A, Vassilev A, Saric A, Griner LN, Marugan J, Ferrer M, Bonifacino JS, DePamphilis ML | display-authors = 6 | title = A family of PIKFYVE inhibitors with therapeutic potential against autophagy-dependent cancer cells disrupt multiple events in lysosome homeostasis | journal = Autophagy | volume = 15 | issue = 10 | pages = 1694–1718 | date = February 2019 | pmid = 30806145 | pmc = 6735543 | doi = 10.1080/15548627.2019.1586257 }} Thus, via PtdIns(3,5)P2 production, PIKfyve participates in several aspects of vesicular dynamics, thereby affecting a number of trafficking pathways that emanate from or traverse the endosomal system en route to the trans-Golgi network or later compartments along the endocytic pathway.

PIKfyve mutations affecting one of the two PIKFYVE alleles are found in 8 out of 10 families with Francois-Neetens corneal fleck dystrophy. Disruption of both PIKFYVE alleles in the mouse is lethal at the stage of pre-implantation embryo. PIKfyve’s role in pathogen invasion is deduced by evidence from cell studies implicating PIKfyve activity in HIV and Salmonella replication. A link of PIKfyve with type 2 diabetes is inferred by the observations that PIKfyve perturbation inhibits insulin-regulated glucose uptake. Concordantly, mice with selective Pikfyve gene disruption in skeletal muscle, the tissue mainly responsible for the decrease of postprandial blood sugar, exhibit systemic insulin resistance; glucose intolerance; hyperinsulinemia; and increased adiposity, i.e. symptoms, typical for human prediabetes.{{cite journal |pmid=23673157|pmc=3725567|year=2013|last1=Ikonomov|first1=O. C.|title=Muscle-specific Pikfyve gene disruption causes glucose intolerance, insulin resistance, adiposity, and hyperinsulinemia but not muscle fiber-type switching|journal=American Journal of Physiology. Endocrinology and Metabolism|volume=305|issue=1|pages=E119-31|last2=Sbrissa|first2=D.|last3=Delvecchio|first3=K.|last4=Feng|first4=H. Z.|last5=Cartee|first5=G. D.|last6=Jin|first6=J. P.|last7=Shisheva|first7=A.|doi=10.1152/ajpendo.00030.2013}}

Several small molecule PIKfyve inhibitors have shown promise as cancer therapeutics in preclinical studies due to selective toxicity in non-Hodgkin lymphoma B cells {{Cite journal|last1=Gayle|first1=S|last2=Landrette|first2=S|last3=Beeharry|first3=N|last4=Conrad|first4=C|last5=Hernandez|first5=M|last6=Beckett|first6=P|last7=Ferguson|first7=SM|last8=Mendelkern|first8=T|last9=Zheng|first9=M|last10=Xu|first10=T|last11=Rothberg|first11=J|last12=Lichenstein|first12=H|title=Identification of apilimod as a first-in-class PIKfyve kinase inhibitor for treatment of B-cell non-Hodgkin lymphoma|journal=Blood |volume=129|issue=13|pages=1768–1778|doi=10.1182/blood-2016-09-736892|pmid=28104689|pmc=5766845|year=2017}} or in U-251 glioblastoma cells. {{Cite journal|last1=Li|first1=Z|last2=Mbah|first2=NE|last3=Overmeyer|first3=JH|last4=Sarver|first4=JG|last5=George|first5=S|last6=Trabbic|first6=CJ|last7=Erhardt|first7=PW|last8=Maltese|first8=WA|title=The JNK signaling pathway plays a key role in methuosis (non-apoptotic cell death) induced by MOMIPP in glioblastoma|journal=BMC Cancer |volume=19|issue=1|pages=77|doi=10.1186/s12885-019-5288-y|pmid=30651087|pmc=6335761|year=2019|doi-access=free}}

PIKfyve inhibitors cause cell death also in A-375 melanoma cells, which depend on autophagy for growth and proliferation, due to impaired lysosome homeostasis. {{cite journal | vauthors = Sharma G, Guardia CM, Roy A, Vassilev A, Saric A, Griner LN, Marugan J, Ferrer M, Bonifacino JS, DePamphilis ML | display-authors = 6 | title = A family of PIKFYVE inhibitors with therapeutic potential against autophagy-dependent cancer cells disrupt multiple events in lysosome homeostasis | journal = Autophagy | volume = 15 | issue = 10 | pages = 1694–1718 | date = February 2019 | pmid = 30806145 | pmc = 6735543 | doi = 10.1080/15548627.2019.1586257 }} The potential therapeutic use of PIKfyve inhibitors awaits clinical trials.

PIKfyve physically associates with its regulator ArPIKfyve, a protein encoded by the human gene VAC14, and the Sac1 domain-containing PtdIns(3,5)P2 5-phosphatase Sac3, encoded by FIG4, to form a stable ternary heterooligomeric complex that is scaffolded by ArPIKfyve homooligomeric interactions. The presence of two enzymes with opposing activities for PtdIns(3,5)P2 synthesis and turnover in a single complex indicates the requirement for a tight control of PtdIns(3,5)P2 levels. PIKfyve also interacts with the Rab9 effector RABEPK and the kinesin adaptor JLP, encoded by SPAG9. These interactions link PIKfyve to microtubule-based endosome to trans-Golgi network traffic. Under sustained activation of glutamate receptors PIKfyve binds to and facilitates the lysosomal degradation of Ca_v1.2, voltage-dependent calcium channel type 1.2, thereby protecting the neurons from excitotoxicity. PIKfyve negatively regulates Ca²⁺-dependent exocytosis in neuroendocrine cells without affecting voltage-gated calcium channels.

PIKFYVE belongs to a large family of evolutionarily-conserved lipid kinases. Single copy genes, encoding similarly-structured FYVE-domain–containing phosphoinositide kinases exist in most genomes from yeast to man. The plant A. thaliana has several copies of the enzyme. Higher eukaryotes (after D. melanogaster), acquire an additional DEP domain. The S. cerevisiae enzyme Fab1p is required for PtdIns(3,5)P2 synthesis under basal conditions and in response to hyperosmotic shock. PtdIns5P, made by PIKfyve kinase activity in mammalian cells, is not detected in budding yeast. Yeast Fab1p associates with Vac14p (the ortholog of human ArPIKfyve) and Fig4p (the ortholog of Sac3). The yeast Fab1 complex also includes Vac7p and probably Atg18p, proteins that are not detected in the mammalian PIKfyve complex. S. cerevisiae could survive without Fab1. In contrast, the knockout of the FYVE domain-containing enzymes in A. thaliana, D. melanogaster, C. elegans and M. musculus leads to embryonic lethality indicating that the FYVE-domain–containing phosphoinositide kinases have become essential in embryonic development of multicellular organisms. Thus, in evolution, the FYVE-domain-containing phosphoinositide kinases retain several aspects of the structural organization, enzyme activity and protein interactions from budding yeast. In higher eukaryotes, the enzymes acquire one additional domain, a role in the production of PtdIns5P, a new set of interacting proteins and become essential in embryonic development.

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{{cite journal | vauthors = Ikonomov OC, Sbrissa D, Shisheva A | title = Mammalian cell morphology and endocytic membrane homeostasis require enzymatically active phosphoinositide 5-kinase PIKfyve | journal = The Journal of Biological Chemistry | volume = 276 | issue = 28 | pages = 26141–7 | date = July 2001 | pmid = 11285266 | doi = 10.1074/jbc.M101722200 | doi-access = free }}

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{{cite journal | vauthors = Sbrissa D, Ikonomov OC, Fenner H, Shisheva A | title = ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality | journal = Journal of Molecular Biology | volume = 384 | issue = 4 | pages = 766–79 | date = December 2008 | pmid = 18950639 | pmc = 2756758 | doi = 10.1016/j.jmb.2008.10.009 }}

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