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SF3A1

{{Short description|Protein-coding gene in the species Homo sapiens}}

{{Infobox_gene}}

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene.{{cite journal |author1=Kramer A |author2=Mulhauser F |author3=Wersig C |author4=Groning K |author5=Bilbe G | title = Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae | journal = RNA | volume = 1 | issue = 3 | pages = 260–72 |date=January 1996 | pmid = 7489498 | pmc = 1369079 }}{{cite web | title = Entrez Gene: SF3A1 splicing factor 3a, subunit 1, 120kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10291}}

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Interactions

SF3A1 has been shown to interact with SF3A3{{cite journal |last=Nesic |first=D |author2=Krämer A |date=October 2001 |title=Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation |journal=Mol. Cell. Biol. |volume=21 |issue=19 |pages=6406–17 |location = United States| issn = 0270-7306| pmid = 11533230 |doi=10.1128/MCB.21.19.6406-6417.2001 |pmc=99788 }}{{cite journal |last=Chiara |first=M D |author2=Champion-Arnaud P |author3=Buvoli M |author4=Nadal-Ginard B |author5=Reed R |date=July 1994 |title=Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114 |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=91 |issue=14 |pages=6403–7 |location = UNITED STATES| issn = 0027-8424| pmid = 8022796 | bibcode =1994PNAS...91.6403C |doi=10.1073/pnas.91.14.6403 |pmc=44210 |doi-access=free }} and CDC5L.{{cite journal |last=Ajuh |first=P |author2=Kuster B |author3=Panov K |author4=Zomerdijk J C |author5=Mann M |author6=Lamond A I |date=Dec 2000 |title=Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry |journal=EMBO J. |volume=19 |issue=23 |pages=6569–81 |location = ENGLAND| issn = 0261-4189| pmid = 11101529 |doi = 10.1093/emboj/19.23.6569 |pmc=305846 }}

References

{{Reflist}}

Further reading

{{Refbegin | 2}}

  • {{cite journal |vauthors=Chiara MD, Champion-Arnaud P, Buvoli M, etal |title=Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=91 |issue= 14 |pages= 6403–7 |year= 1994 |pmid= 8022796 |doi=10.1073/pnas.91.14.6403 | pmc=44210 |bibcode=1994PNAS...91.6403C |doi-access=free }}
  • {{cite journal |author1=Rain JC |author2=Tartakoff AM |author3=Krämer A |author4=Legrain P |title=Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. |journal=RNA |volume=2 |issue= 6 |pages= 535–50 |year= 1996 |pmid= 8718683 | pmc=1369393 }}
  • {{cite journal |vauthors=Neubauer G, King A, Rappsilber J, etal |title=Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. |journal=Nat. Genet. |volume=20 |issue= 1 |pages= 46–50 |year= 1998 |pmid= 9731529 |doi= 10.1038/1700 |s2cid=585778 }}
  • {{cite journal |title=Toward a complete human genome sequence. |journal=Genome Res. |volume=8 |issue= 11 |pages= 1097–108 |year= 1999 |pmid= 9847074 |doi= 10.1101/gr.8.11.1097|doi-access=free |last1=Sanger Centre |first1=The |last2=Washington University Genome Sequencing Cente |first2=The }}
  • {{cite journal |author1=Krämer A |author2=Grüter P |author3=Gröning K |author4=Kastner B |title=Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP. |journal=J. Cell Biol. |volume=145 |issue= 7 |pages= 1355–68 |year= 1999 |pmid= 10385517 |doi=10.1083/jcb.145.7.1355 | pmc=2133165 }}
  • {{cite journal |vauthors=Dunham I, Shimizu N, Roe BA, etal |title=The DNA sequence of human chromosome 22. |journal=Nature |volume=402 |issue= 6761 |pages= 489–95 |year= 1999 |pmid= 10591208 |doi= 10.1038/990031 |bibcode=1999Natur.402..489D |doi-access=free }}
  • {{cite journal |author1=Das R |author2=Zhou Z |author3=Reed R |title=Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. |journal=Mol. Cell |volume=5 |issue= 5 |pages= 779–87 |year= 2000 |pmid= 10882114 |doi=10.1016/S1097-2765(00)80318-4 |doi-access=free }}
  • {{cite journal |author1=Gunther M |author2=Laithier M |author3=Brison O |title=A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening. |journal=Mol. Cell. Biochem. |volume=210 |issue= 1–2 |pages= 131–42 |year= 2000 |pmid= 10976766 |doi=10.1023/A:1007177623283 |s2cid=1339642 }}
  • {{cite journal |vauthors=Ajuh P, Kuster B, Panov K, etal |title=Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry. |journal=EMBO J. |volume=19 |issue= 23 |pages= 6569–81 |year= 2001 |pmid= 11101529 |doi= 10.1093/emboj/19.23.6569 | pmc=305846 }}
  • {{cite journal |vauthors=Suzuki Y, Tsunoda T, Sese J, etal |title=Identification and characterization of the potential promoter regions of 1031 kinds of human genes. |journal=Genome Res. |volume=11 |issue= 5 |pages= 677–84 |year= 2001 |pmid= 11337467 |doi= 10.1101/gr.gr-1640r| pmc=311086 }}
  • {{cite journal |vauthors=Will CL, Schneider C, MacMillan AM, etal |title=A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site. |journal=EMBO J. |volume=20 |issue= 16 |pages= 4536–46 |year= 2001 |pmid= 11500380 |doi= 10.1093/emboj/20.16.4536 | pmc=125580 }}
  • {{cite journal |author1=Nesic D |author2=Krämer A |title=Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation. |journal=Mol. Cell. Biol. |volume=21 |issue= 19 |pages= 6406–17 |year= 2001 |pmid= 11533230 |doi=10.1128/MCB.21.19.6406-6417.2001 | pmc=99788 }}
  • {{cite journal |vauthors=Jurica MS, Licklider LJ, Gygi SR, etal |title=Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. |journal=RNA |volume=8 |issue= 4 |pages= 426–39 |year= 2002 |pmid= 11991638 |doi=10.1017/S1355838202021088 | pmc=1370266 }}
  • {{cite journal |vauthors=Will CL, Urlaub H, Achsel T, etal |title=Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein. |journal=EMBO J. |volume=21 |issue= 18 |pages= 4978–88 |year= 2002 |pmid= 12234937 |doi=10.1093/emboj/cdf480 | pmc=126279 }}
  • {{cite journal |vauthors=Strausberg RL, Feingold EA, Grouse LH, etal |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 |bibcode=2002PNAS...9916899M |doi-access=free }}
  • {{cite journal |vauthors=Beausoleil SA, Jedrychowski M, Schwartz D, etal |title=Large-scale characterization of HeLa cell nuclear phosphoproteins. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=101 |issue= 33 |pages= 12130–5 |year= 2004 |pmid= 15302935 |doi= 10.1073/pnas.0404720101 | pmc=514446 |bibcode=2004PNAS..10112130B |doi-access=free }}
  • {{cite journal |author1=Nesic D |author2=Tanackovic G |author3=Krämer A |title=A role for Cajal bodies in the final steps of U2 snRNP biogenesis. |journal=J. Cell Sci. |volume=117 |issue= Pt 19 |pages= 4423–33 |year= 2005 |pmid= 15316075 |doi= 10.1242/jcs.01308 |url=https://archive-ouverte.unige.ch/unige:17542/ATTACHMENT01 |doi-access=free }}
  • {{cite journal |author1=Lin KT |author2=Lu RM |author3=Tarn WY |title=The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo. |journal=Mol. Cell. Biol. |volume=24 |issue= 20 |pages= 9176–85 |year= 2004 |pmid= 15456888 |doi= 10.1128/MCB.24.20.9176-9185.2004 | pmc=517884 }}
  • {{cite journal |vauthors=Collins JE, Wright CL, Edwards CA, etal |title=A genome annotation-driven approach to cloning the human ORFeome. |journal=Genome Biol. |volume=5 |issue= 10 |pages= R84 |year= 2005 |pmid= 15461802 |doi= 10.1186/gb-2004-5-10-r84 | pmc=545604 |doi-access=free }}

{{Refend}}

External links

  • {{UCSC genome browser|SF3A1}}
  • {{UCSC gene details|SF3A1}}

{{PDB Gallery|geneid=10291}}

{{Gene-22-stub}}