Small nucleolar RNA SNORD66

{{Infobox rfam

| Name = Small nucleolar RNA SNORD66

| image = RF00572.jpg

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| caption = Predicted secondary structure and sequence conservation of SNORD66

| Symbol = SNORD66

| AltSymbols = snoHBII-142

| Rfam = RF00572

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| RNA_type = Gene; snRNA; snoRNA; C/D-box

| Tax_domain = Eukaryota

| GO = {{GO|0006396}} {{GO|0005730}}

| SO = {{SO|0000593}}

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In molecular biology, SNORD66 (also known as HBII-142) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

HBII-142 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs.{{cite journal | vauthors = Galardi S, Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I | title = Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro | journal = Molecular and Cellular Biology | volume = 22 | issue = 19 | pages = 6663–8 | date = October 2002 | pmid = 12215523 | pmc = 134041 | doi = 10.1128/MCB.22.19.6663-6668.2002 }}

HBII-142 is the human orthologue of the mouse MBII-142 snoRNA{{cite journal | vauthors = Hüttenhofer A, Kiefmann M, Meier-Ewert S, O'Brien J, Lehrach H, Bachellerie JP, Brosius J | title = RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse | journal = The EMBO Journal | volume = 20 | issue = 11 | pages = 2943–53 | date = June 2001 | pmid = 11387227 | pmc = 125495 | doi = 10.1093/emboj/20.11.2943 }} and is predicted to guide 2'O-ribose methylation of 18S ribosomal RNA (rRNA) at residue C1272.{{cite journal | vauthors = Lestrade L, Weber MJ | title = snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs | journal = Nucleic Acids Research | volume = 34 | issue = Database issue | pages = D158-62 | date = January 2006 | pmid = 16381836 | pmc = 1347365 | doi = 10.1093/nar/gkj002 | citeseerx = 10.1.1.105.7552 }}

An experiment that looked at 22 different non-small-cell lung cancer tissues found that SNORD33, SNORD66 and SNORD76 were over-expressed relative to matched noncancerous lung tissues.{{cite journal | vauthors = Liao J, Yu L, Mei Y, Guarnera M, Shen J, Li R, Liu Z, Jiang F | title = Small nucleolar RNA signatures as biomarkers for non-small-cell lung cancer | journal = Molecular Cancer | volume = 9 | pages = 198 | date = July 2010 | pmid = 20663213 | pmc = 2919450 | doi = 10.1186/1476-4598-9-198 | doi-access = free }}