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UBE1C

{{Short description|Protein-coding gene in the species Homo sapiens}}

{{Infobox_gene}}

{{Infobox protein family

| Symbol = E2_bind

| Name = E2 binding domain

| image = PDB 1r4n EBI.jpg

| width =

| caption = appbp1-uba3-nedd8, an e1-ubiquitin-like protein complex with atp

| Pfam = PF08825

| Pfam_clan =

| InterPro = IPR014929

| SMART =

| PROSITE =

| MEROPS =

| SCOP =

| TCDB =

| OPM family =

| OPM protein =

| CAZy =

| CDD =

}}

NEDD8-activating enzyme E1 catalytic subunit is a protein that in humans is encoded by the UBA3 gene.{{cite journal |vauthors=Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S | title = A new NEDD8-ligating system for cullin-4A | journal = Genes Dev | volume = 12 | issue = 15 | pages = 2263–8 |date=August 1998 | pmid = 9694792 | pmc = 317039 | doi =10.1101/gad.12.15.2263 }}{{cite web | title = Entrez Gene: UBE1C ubiquitin-activating enzyme E1C (UBA3 homolog, yeast)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9039}}

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

This enzyme contains an E2 binding domain, which resembles ubiquitin, and recruits the catalytic core of the E2 enzyme UBE2M (Ubc12) in a similar manner to that in which ubiquitin interacts with ubiquitin binding domains.{{cite journal |vauthors=Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA | title = Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1 | journal = Mol. Cell | volume = 17 | issue = 3 | pages = 341–50 |date=February 2005 | pmid = 15694336 | doi = 10.1016/j.molcel.2004.12.020 | doi-access = free }}

Interactions

UBE1C has been shown to interact with NEDD8,{{cite journal |doi=10.1074/jbc.274.17.12036 |last=Gong |first=L |author2=Yeh E T |date=April 1999 |title=Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway |journal=J. Biol. Chem. |volume=274 |issue=17 |pages=12036–42 |location = UNITED STATES| issn = 0021-9258| pmid = 10207026 |doi-access=free }} APPBP1{{cite journal |doi=10.1074/jbc.275.12.8929 |last=Chen |first=Y |author2=McPhie D L |author3=Hirschberg J |author4=Neve R L |date=March 2000 |title=The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons |journal=J. Biol. Chem. |volume=275 |issue=12 |pages=8929–35 |location = UNITED STATES| issn = 0021-9258| pmid = 10722740 |doi-access=free }} and UBE2M.

References

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Further reading

{{refbegin | 2}}

  • {{cite journal |vauthors=Gong L, Yeh ET |title=Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. |journal=J. Biol. Chem. |volume=274 |issue= 17 |pages= 12036–42 |year= 1999 |pmid= 10207026 |doi=10.1074/jbc.274.17.12036 |doi-access=free }}
  • {{cite journal |vauthors=Gubin AN, Njoroge JM, Bouffard GG, Miller JL |title=Gene expression in proliferating human erythroid cells. |journal=Genomics |volume=59 |issue= 2 |pages= 168–77 |year= 1999 |pmid= 10409428 |doi= 10.1006/geno.1999.5855 |url= https://zenodo.org/record/1229786 }}
  • {{cite journal |vauthors=Chen Y, McPhie DL, Hirschberg J, Neve RL |title=The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons. |journal=J. Biol. Chem. |volume=275 |issue= 12 |pages= 8929–35 |year= 2000 |pmid= 10722740 |doi=10.1074/jbc.275.12.8929 |doi-access=free }}
  • {{cite journal |vauthors=Hartley JL, Temple GF, Brasch MA |title=DNA cloning using in vitro site-specific recombination. |journal=Genome Res. |volume=10 |issue= 11 |pages= 1788–95 |year= 2001 |pmid= 11076863 |doi=10.1101/gr.143000 | pmc=310948 }}
  • {{cite journal |vauthors=Wiemann S, Weil B, Wellenreuther R, etal |title=Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. |journal=Genome Res. |volume=11 |issue= 3 |pages= 422–35 |year= 2001 |pmid= 11230166 |doi= 10.1101/gr.GR1547R | pmc=311072 }}
  • {{cite journal |vauthors=Simpson JC, Wellenreuther R, Poustka A, etal |title=Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. |journal=EMBO Rep. |volume=1 |issue= 3 |pages= 287–92 |year= 2001 |pmid= 11256614 |doi= 10.1093/embo-reports/kvd058 | pmc=1083732 }}
  • {{cite journal |vauthors=Strausberg RL, Feingold EA, Grouse LH, etal |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 |bibcode=2002PNAS...9916899M |doi-access=free }}
  • {{cite journal |vauthors=Walden H, Podgorski MS, Schulman BA |title=Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8. |journal=Nature |volume=422 |issue= 6929 |pages= 330–4 |year= 2003 |pmid= 12646924 |doi= 10.1038/nature01456 |bibcode=2003Natur.422..330W |s2cid=4370095 }}
  • {{cite journal |vauthors=Bohnsack RN, Haas AL |title=Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. |journal=J. Biol. Chem. |volume=278 |issue= 29 |pages= 26823–30 |year= 2003 |pmid= 12740388 |doi= 10.1074/jbc.M303177200 |doi-access= free }}
  • {{cite journal |vauthors=Walden H, Podgorski MS, Huang DT, etal |title=The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1. |journal=Mol. Cell |volume=12 |issue= 6 |pages= 1427–37 |year= 2004 |pmid= 14690597 |doi=10.1016/S1097-2765(03)00452-0 |doi-access=free }}
  • {{cite journal |vauthors=Ota T, Suzuki Y, Nishikawa T, etal |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40–5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 |doi-access= free }}
  • {{cite journal |vauthors=Huang DT, Miller DW, Mathew R, etal |title=A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. |journal=Nat. Struct. Mol. Biol. |volume=11 |issue= 10 |pages= 927–35 |year= 2004 |pmid= 15361859 |doi= 10.1038/nsmb826 | pmc=2862556 }}
  • {{cite journal |vauthors=Gerhard DS, Wagner L, Feingold EA, etal |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 | pmc=528928 }}
  • {{cite journal |vauthors=Wiemann S, Arlt D, Huber W, etal |title=From ORFeome to biology: a functional genomics pipeline. |journal=Genome Res. |volume=14 |issue= 10B |pages= 2136–44 |year= 2004 |pmid= 15489336 |doi= 10.1101/gr.2576704 | pmc=528930 }}
  • {{cite journal |vauthors=Huang DT, Paydar A, Zhuang M, etal |title=Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. |journal=Mol. Cell |volume=17 |issue= 3 |pages= 341–50 |year= 2005 |pmid= 15694336 |doi= 10.1016/j.molcel.2004.12.020 |doi-access= free }}
  • {{cite journal |vauthors=Mehrle A, Rosenfelder H, Schupp I, etal |title=The LIFEdb database in 2006. |journal=Nucleic Acids Res. |volume=34 |issue= Database issue |pages= D415–8 |year= 2006 |pmid= 16381901 |doi= 10.1093/nar/gkj139 | pmc=1347501 }}
  • {{cite journal |vauthors=Hiller M, Huse K, Szafranski K, etal |title=Single-nucleotide polymorphisms in NAGNAG acceptors are highly predictive for variations of alternative splicing. |journal=Am. J. Hum. Genet. |volume=78 |issue= 2 |pages= 291–302 |year= 2007 |pmid= 16400609 |doi= 10.1086/500151 | pmc=1380236 }}
  • {{cite journal |vauthors=Norman JA, Shiekhattar R |title=Analysis of Nedd8-associated polypeptides: a model for deciphering the pathway for ubiquitin-like modifications. |journal=Biochemistry |volume=45 |issue= 9 |pages= 3014–9 |year= 2006 |pmid= 16503656 |doi= 10.1021/bi052435a }}
  • {{cite journal |vauthors=Li T, Santockyte R, Shen RF, etal |title=A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers. |journal=Arch. Biochem. Biophys. |volume=453 |issue= 1 |pages= 70–4 |year= 2006 |pmid= 16620772 |doi= 10.1016/j.abb.2006.03.002 |url=https://zenodo.org/record/1258674 }}

{{refend}}

External links

  • [https://www.ebi.ac.uk/pdbe/pdbe-kb/proteins/Q8TBC4 PDBe-KB] provides an overview of all the structure information available in the PDB for Human NEDD8-activating enzyme E1 catalytic subunit (UBE1C)

{{PDB Gallery|geneid=9039}}

{{InterPro content|IPR014929}}

Category:Protein domains

{{gene-3-stub}}