UBE2M

{{Short description|Protein-coding gene in the species Homo sapiens}}

{{Infobox_gene}}

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.{{cite journal |vauthors=Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S | title = A new NEDD8-ligating system for cullin-4A | journal = Genes Dev | volume = 12 | issue = 15 | pages = 2263–8 |date=Aug 1998 | pmid = 9694792 | pmc = 317039 | doi =10.1101/gad.12.15.2263 }}{{cite web | title = Entrez Gene: UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9040}}

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Interactions

UBE2M has been shown to interact with NEDD8,{{cite journal |vauthors=Gong L, Yeh ET |date=Apr 1999 |title=Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway |journal=J. Biol. Chem. |volume=274 |issue=17 |pages=12036–42 |location = UNITED STATES| issn = 0021-9258| pmid = 10207026 |doi=10.1074/jbc.274.17.12036 |doi-access=free }} PRKAR1A{{cite journal |vauthors=Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D |year=2007|title=Large-scale mapping of human protein–protein interactions by mass spectrometry |journal=Mol. Syst. Biol. |volume=3 |issue= 1|pages=89 |location = England| pmid = 17353931 |doi = 10.1038/msb4100134 |pmc=1847948 }} and UBA3.{{cite journal |vauthors=Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA | title = Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1 | journal = Mol. Cell | volume = 17 | issue = 3 | pages = 341–50 |date=February 2005 | pmid = 15694336 | doi = 10.1016/j.molcel.2004.12.020 | doi-access = free }}

References

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Further reading

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  • {{cite journal |vauthors=Gong L, Yeh ET |title=Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway |journal=J. Biol. Chem. |volume=274 |issue= 17 |pages= 12036–42 |year= 1999 |pmid= 10207026 |doi=10.1074/jbc.274.17.12036 |doi-access=free }}
  • {{cite journal |vauthors=Chen Y, McPhie DL, Hirschberg J, Neve RL |title=The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons |journal=J. Biol. Chem. |volume=275 |issue= 12 |pages= 8929–35 |year= 2000 |pmid= 10722740 |doi=10.1074/jbc.275.12.8929 |doi-access=free }}
  • {{cite journal |vauthors=Wada H, Yeh ET, Kamitani T |title=A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo |journal=J. Biol. Chem. |volume=275 |issue= 22 |pages= 17008–15 |year= 2000 |pmid= 10828074 |doi=10.1074/jbc.275.22.17008 |doi-access=free }}
  • {{cite journal | author=Strausberg RL |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 |name-list-style=vanc| author2=Feingold EA | author3=Grouse LH | display-authors=3 | last4=Derge | first4=JG | last5=Klausner | first5=RD | last6=Collins | first6=FS | last7=Wagner | first7=L | last8=Shenmen | first8=CM | last9=Schuler | first9=GD |bibcode=2002PNAS...9916899M|doi-access=free }}
  • {{cite journal |vauthors=Tomsig JL, Snyder SL, Creutz CE |title=Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif |journal=J. Biol. Chem. |volume=278 |issue= 12 |pages= 10048–54 |year= 2003 |pmid= 12522145 |doi= 10.1074/jbc.M212632200 |doi-access= free }}
  • {{cite journal | author=Huang DT |title=A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8 |journal=Nat. Struct. Mol. Biol. |volume=11 |issue= 10 |pages= 927–35 |year= 2004 |pmid= 15361859 |doi= 10.1038/nsmb826 | pmc=2862556 |name-list-style=vanc| author2=Miller DW | author3=Mathew R | display-authors=3 | last4=Cassell | first4=Robert | last5=Holton | first5=James M | last6=Roussel | first6=Martine F | last7=Schulman | first7=Brenda A }}
  • {{cite journal | author=Gerhard DS |title=The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 | pmc=528928 |name-list-style=vanc| author2=Wagner L | author3=Feingold EA | display-authors=3 | last4=Shenmen | first4=CM | last5=Grouse | first5=LH | last6=Schuler | first6=G | last7=Klein | first7=SL | last8=Old | first8=S | last9=Rasooly | first9=R }}
  • {{cite journal | author=Huang DT |title=Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1 |journal=Mol. Cell |volume=17 |issue= 3 |pages= 341–50 |year= 2005 |pmid= 15694336 |doi= 10.1016/j.molcel.2004.12.020 |name-list-style=vanc| author2=Paydar A | author3=Zhuang M | display-authors=3 | last4=Waddell | first4=M.Brett | last5=Holton | first5=James M. | last6=Schulman | first6=Brenda A. | doi-access=free}}
  • {{cite journal | author=Li T |title=A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers |journal=Arch. Biochem. Biophys. |volume=453 |issue= 1 |pages= 70–4 |year= 2006 |pmid= 16620772 |doi= 10.1016/j.abb.2006.03.002 |name-list-style=vanc| author2=Santockyte R | author3=Shen RF | display-authors=3 | last4=Tekle | first4=E | last5=Wang | first5=G | last6=Yang | first6=D | last7=Chock | first7=P |url=https://zenodo.org/record/1258674 }}
  • {{cite journal | author=Ewing RM |title=Large-scale mapping of human protein–protein interactions by mass spectrometry |journal=Mol. Syst. Biol. |volume=3 |issue= 1|pages= 89 |year= 2007 |pmid= 17353931 |doi= 10.1038/msb4100134 | pmc=1847948 |name-list-style=vanc| author2=Chu P | author3=Elisma F | display-authors=3 | last4=Li | first4=Hongyan | last5=Taylor | first5=Paul | last6=Climie | first6=Shane | last7=McBroom-Cerajewski | first7=Linda | last8=Robinson | first8=Mark D | last9=O'Connor | first9=Liam }}

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{{PDB Gallery|geneid=9040}}

{{Ubiquitin-conjugating enzymes}}

{{gene-19-stub}}