User:Rockpocket/MGP/ARID2
==Model organisms==
Model organisms have been used in the study of ARID2 function. A conditional knockout mouse line, called Arid2tm1a(EUCOMM)Wtsi{{cite web |url=http://www.knockoutmouse.org/martsearch/search?query=Arid2 |title=International Knockout Mouse Consortium}}{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4433303 |title=Mouse Genome Informatics}} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.{{Cite journal| last1 = Skarnes |first1 =W. C.| doi = 10.1038/nature10163 | last2 = Rosen | first2 = B.| last3 = West | first3 = A. P.| last4 = Koutsourakis | first4 = M.| last5 = Bushell | first5 = W.| last6 = Iyer | first6 = V.| last7 = Mujica | first7 = A. O.| last8 = Thomas | first8 = M.| last9 = Harrow | first9 = J.| last10 = Cox | first10 = T.| last11 = Jackson | first11 = D.| last12 = Severin | first12 = J.| last13 = Biggs | first13 = P.| last14 = Fu | first14 = J.| last15 = Nefedov | first15 = M.| last16 = De Jong | first16 = P. J.| last17 = Stewart | first17 = A. F.| last18 = Bradley | first18 = A. | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–342 | year = 2011 | pmid = 21677750 | pmc =3572410 }}{{cite web |url=http://www.nature.com/news/2011/110615/full/474262a.html |title=Mouse library set to be knockout |author=Dolgin E |year=June 2011 |location=Nature 474: 262-263. doi:10.1038/474262a}}{{cite book |title=A mouse for all reasons |author=Collins FS, Rossant J, Wurst W |year=January 2007 |location=Cell 128(1): 9-13. doi:10.1016/j.cell.2006.12.018 PMID 17218247}}
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.{{cite journal| author=van der Weyden L, White JK, Adams DJ, Logan DW| title=The mouse genetics toolkit: revealing function and mechanism. | journal=Genome Biol | year= 2011 | volume= 12 | issue= 6 | pages= 224 | pmid=21722353 | doi=10.1186/gb-2011-12-6-224 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=21722353 }}
| class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: right;" |
|+ Arid2 knockout mouse phenotype | |
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | bgcolor="#C40000"|Abnormal |
| Recessive lethal study | bgcolor="#C40000"|Abnormal |
| Fertility | bgcolor="#488ED3"|Normal |
| Body weight | bgcolor="#488ED3"|Normal |
| Anxiety | bgcolor="#488ED3"|Normal |
| Neurological assessment | bgcolor="#488ED3"|Normal |
| Grip strength | bgcolor="#488ED3"|Normal |
| Hot plate | bgcolor="#488ED3"|Normal |
| Dysmorphology | bgcolor="#488ED3"|Normal |
| Indirect calorimetry | bgcolor="#488ED3"|Normal |
| Glucose tolerance test | bgcolor="#488ED3"|Normal |
| Auditory brainstem response | bgcolor="#488ED3"|Normal |
| DEXA | bgcolor="#488ED3"|Normal |
| Radiography | bgcolor="#488ED3"|Normal |
| Body temperature | bgcolor="#488ED3"|Normal |
| Eye morphology | bgcolor="#488ED3"|Normal |
| Clinical chemistry | bgcolor="#488ED3"|Normal |
| Haematology | bgcolor="#488ED3"|Normal |
| Peripheral blood lymphocytes | bgcolor="#488ED3"|Normal |
| Micronucleus test | bgcolor="#488ED3"|Normal |
| Heart weight | bgcolor="#488ED3"|Normal |
| Skin Histopathology | bgcolor="#488ED3"|Normal |
| Brain histopathology | bgcolor="#488ED3"|Normal |
| Eye Histopathology | bgcolor="#488ED3"|Normal |
| Salmonella infection | bgcolor="#488ED3"|Normal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAEV/salmonella-challenge/ |title=Salmonella infection data for Arid2 |publisher=Wellcome Trust Sanger Institute}} |
| Citrobacter infection | bgcolor="#488ED3"|Normal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAEV/citrobacter-challenge/ |title=Citrobacter infection data for Arid2 |publisher=Wellcome Trust Sanger Institute}} |
| colspan=2; style="text-align: center;" | All tests and analysis from{{cite web |url=http://onlinelibrary.wiley.com/doi/10.1111/j.1755-3768.2010.4142.x/abstract |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |location=Acta Opthalmologica 88: 925-7.doi:10.1111/j.1755-3768.2010.4142.x |publisher=Wiley}} |
Twenty six tests were carried out on mutant adult mice and two significant abnormalities were observed. A recessive lethal study found less homozygous mutant embryos during gestation than predicted by Mendelian ratio. In a second study, no homozygous mutant animals survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; these displayed no abnormalities.
References
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