User:Rockpocket/MGP/CADM1
==Model organisms==
| class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: right;" |
|+ Cadm1 knockout mouse phenotype | |
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | bgcolor="#488ED3"|Normal |
| Fertility | bgcolor="#C40000"|Abnormal |
| Body weight | bgcolor="#488ED3"|Normal |
| Anxiety | bgcolor="#488ED3"|Normal |
| Neurological assessment | bgcolor="#488ED3"|Normal |
| Grip strength | bgcolor="#488ED3"|Normal |
| Hot plate | bgcolor="#488ED3"|Normal |
| Dysmorphology | bgcolor="#488ED3"|Normal |
| Rotarod | bgcolor="#488ED3"|Normal |
| Non-Invasive Blood Pressure | bgcolor="#488ED3"|Normal |
| Prepulse inhibition | bgcolor="#488ED3"|Normal |
| Indirect calorimetry | bgcolor="#488ED3"|Normal |
| Glucose tolerance test | bgcolor="#488ED3"|Normal |
| Auditory brainstem response | bgcolor="#488ED3"|Normal |
| DEXA | bgcolor="#488ED3"|Normal |
| Radiography | bgcolor="#488ED3"|Normal |
| Body temperature | bgcolor="#488ED3"|Normal |
| Eye morphology | bgcolor="#488ED3"|Normal |
| Clinical chemistry | bgcolor="#488ED3"|Normal |
| Haematology | bgcolor="#488ED3"|Normal |
| Micronucleus test | bgcolor="#488ED3"|Normal |
| Heart weight | bgcolor="#488ED3"|Normal |
| Tail epidermis wholemount | bgcolor="#488ED3"|Normal |
| Brain histopathology | bgcolor="#488ED3"|Normal |
| Salmonella infection | bgcolor="#488ED3"|Normal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAMQ/salmonella-challenge/ |title=Salmonella infection data for Cadm1 |publisher=Wellcome Trust Sanger Institute}} |
| Citrobacter infection | bgcolor="#488ED3"|Normal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MAMQ/citrobacter-challenge/ |title=Citrobacter infection data for Cadm1 |publisher=Wellcome Trust Sanger Institute}} |
| colspan=2; style="text-align: center;" | All tests and analysis from{{cite web |url=http://onlinelibrary.wiley.com/doi/10.1111/j.1755-3768.2010.4142.x/abstract |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |location=Acta Opthalmologica 88: 925-7.doi:10.1111/j.1755-3768.2010.4142.x |publisher=Wiley}} |
Model organisms have been used in the study of CADM1 function. A conditional knockout mouse line, called Cadm1tm1.2Brd{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:3625158 |title=Mouse Genome Informatics}} was generated. {{Cite journal | last1 = Van Der Weyden | first1 = L. | last2 = Arends | first2 = M. J. | last3 = Chausiaux | first3 = O. E. | last4 = Ellis | first4 = P. J. | last5 = Lange | first5 = U. C. | last6 = Surani | first6 = M. A. | last7 = Affara | first7 = N. | last8 = Murakami | first8 = Y. | last9 = Adams | first9 = D. J. | doi = 10.1128/MCB.26.9.3595-3609.2006 | last10 = Bradley | first10 = A. | title = Loss of TSLC1 Causes Male Infertility Due to a Defect at the Spermatid Stage of Spermatogenesis | journal = Molecular and Cellular Biology | volume = 26 | issue = 9 | pages = 3595–3609 | year = 2006 | pmid = 16611999 | pmc =1447413 }} Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.{{cite journal| author=van der Weyden L, White JK, Adams DJ, Logan DW| title=The mouse genetics toolkit: revealing function and mechanism. | journal=Genome Biol | year= 2011 | volume= 12 | issue= 6 | pages= 224 | pmid=21722353 | doi=10.1186/gb-2011-12-6-224 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=21722353 }} Twenty six tests were carried out on homozygous mutant mice and one significant abnormality was observed: males were infertile. Further analysis showed spermatogenesis had arrested in these mice.
References
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