User:Rockpocket/MGP/DUSP3

==Model organisms==

class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: right;" | |+ Dusp3 knockout mouse phenotype
Characteristic	Phenotype
Homozygote viability	bgcolor="#488ED3"|Normal
Fertility	bgcolor="#488ED3"|Normal
Body weight	bgcolor="#488ED3"|Normal
Anxiety	bgcolor="#488ED3"|Normal
Neurological assessment	bgcolor="#488ED3"|Normal
Grip strength	bgcolor="#488ED3"|Normal
Hot plate	bgcolor="#488ED3"|Normal
Dysmorphology	bgcolor="#488ED3"|Normal
Indirect calorimetry	bgcolor="#488ED3"|Normal
Glucose tolerance test	bgcolor="#488ED3"|Normal
Auditory brainstem response	bgcolor="#488ED3"|Normal
DEXA	bgcolor="#C40000"|Abnormal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBVF/body-composition-dexa/ |title=DEXA data for Dusp3 |publisher=Wellcome Trust Sanger Institute}}
Radiography	bgcolor="#C40000"|Abnormal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBVF/x-ray-imaging/ |title=Radiography data for Dusp3 |publisher=Wellcome Trust Sanger Institute}}
Body temperature	bgcolor="#488ED3"|Normal
Eye morphology	bgcolor="#488ED3"|Normal
Clinical chemistry	bgcolor="#488ED3"|Normal
Plasma immunoglobulins	bgcolor="#488ED3"|Normal
Haematology	bgcolor="#488ED3"|Normal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBVF/haematology-cbc/ |title=Haematology data for Dusp3 |publisher=Wellcome Trust Sanger Institute}}
Peripheral blood lymphocytes	bgcolor="#488ED3"|Normal
Micronucleus test	bgcolor="#488ED3"|Normal
Heart weight	bgcolor="#488ED3"|Normal
Skin Histopathology	bgcolor="#488ED3"|Normal
Brain histopathology	bgcolor="#C40000"|Abnormal
Salmonella infection	bgcolor="#C40000"|Abnormal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBVF/salmonella-challenge/ |title=Salmonella infection data for Dusp3 |publisher=Wellcome Trust Sanger Institute}}
Citrobacter infection	bgcolor="#488ED3"|Normal{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBVF/citrobacter-challenge/ |title=Citrobacter infection data for Dusp3 |publisher=Wellcome Trust Sanger Institute}}
colspan=2; style="text-align: center;" | All tests and analysis from{{cite web |url=http://onlinelibrary.wiley.com/doi/10.1111/j.1755-3768.2010.4142.x/abstract |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |location=Acta Opthalmologica 88: 925-7.doi:10.1111/j.1755-3768.2010.4142.x |publisher=Wiley}}[http://www.sanger.ac.uk/mouseportal/ Mouse Resources Portal], Wellcome Trust Sanger Institute.

Model organisms have been used in the study of DUSP3 function. A conditional knockout mouse line, called Dusp3^{tm1a(KOMP)Wtsi}{{cite web |url=http://www.knockoutmouse.org/martsearch/search?query=Dusp3 |title=International Knockout Mouse Consortium}}{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4365004 |title=Mouse Genome Informatics}} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.{{Cite journal| last1 = Skarnes |first1 =W. C.| doi = 10.1038/nature10163 | last2 = Rosen | first2 = B.| last3 = West | first3 = A. P.| last4 = Koutsourakis | first4 = M.| last5 = Bushell | first5 = W.| last6 = Iyer | first6 = V.| last7 = Mujica | first7 = A. O.| last8 = Thomas | first8 = M.| last9 = Harrow | first9 = J.| last10 = Cox | first10 = T.| last11 = Jackson | first11 = D.| last12 = Severin | first12 = J.| last13 = Biggs | first13 = P.| last14 = Fu | first14 = J.| last15 = Nefedov | first15 = M.| last16 = De Jong | first16 = P. J.| last17 = Stewart | first17 = A. F.| last18 = Bradley | first18 = A. | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–342 | year = 2011 | pmid = 21677750 | pmc =3572410 }}{{cite web |url=http://www.nature.com/news/2011/110615/full/474262a.html |title=Mouse library set to be knockout |author=Dolgin E |year=June 2011 |location=Nature 474: 262-263. doi:10.1038/474262a}}{{cite book |title=A mouse for all reasons |author=Collins FS, Rossant J, Wurst W |year=January 2007 |location=Cell 128(1): 9-13. doi:10.1016/j.cell.2006.12.018 PMID 17218247}}

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.{{cite journal| author=van der Weyden L, White JK, Adams DJ, Logan DW| title=The mouse genetics toolkit: revealing function and mechanism. | journal=Genome Biol | year= 2011 | volume= 12 | issue= 6 | pages= 224 | pmid=21722353 | doi=10.1186/gb-2011-12-6-224 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=21722353 }} Twenty five tests were carried out on mutant mice and four significant abnormalities were observed. Homozygous mutants had an increased percent of body fat, abnormal humerus morphology and an increased susceptibility to bacterial infection. Corpus callosum area, hippocampus area and total brain section area was increased, while length of pyramidal cell layer was reduced.