| class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: right;" |
|+ Nsun2 knockout mouse phenotype |
| Characteristic | Phenotype |
|---|
| Homozygote viability | bgcolor="#C40000"|Abnormal |
| Recessive lethal study | bgcolor="#488ED3"|Normal |
| Homozygous Fertility | bgcolor="#C40000"|Abnormal |
| Body weight | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/weight-curves/ |title=Body weight data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Anxiety | bgcolor="#488ED3"|Normal |
| Neurological assessment | bgcolor="#488ED3"|Normal |
| Grip strength | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/grip-strength/ |title=Grip strength data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Hot plate | bgcolor="#488ED3"|Normal |
| Dysmorphology | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/dysmorphology/ |title=Dysmorphology data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Indirect calorimetry | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/indirect-calorimetry/ |title=Indirect calorimetry data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Glucose tolerance test | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/glucose-tolerance-ip/ |title=Glucose tolerance test data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Auditory brainstem response | bgcolor="#488ED3"|Normal |
| DEXA | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/body-composition-dexa/ |title=DEXA data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Radiography | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/x-ray-imaging/ |title=Radiography data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Body temperature | bgcolor="#488ED3"|Normal |
| Eye morphology | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/eye-morphology/ |title=Eye morphology data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Clinical chemistry | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/plasma-chemistry/ |title=Clinical chemistry data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Plasma immunoglobulins | bgcolor="#488ED3"|Normal |
| Haematology | bgcolor="#C40000"|Abnormal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/haematology-cbc/ |title=Haematology data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Peripheral blood lymphocytes | bgcolor="#488ED3"|Normal |
| Micronucleus test | bgcolor="#488ED3"|Normal |
| Heart weight | bgcolor="#488ED3"|Normal |
| Tail epidermis wholemount | bgcolor="#488ED3"|Normal |
| Skin Histopathology | bgcolor="#C40000"|Abnormal |
| Brain histopathology | bgcolor="#488ED3"|Normal |
| MicroCT & Quantitative Faxitron | bgcolor="#C40000"|Abnormal |
| Salmonella infection | bgcolor="#488ED3"|Normal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/salmonella-challenge/ |title=Salmonella infection data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
| Citrobacter infection | bgcolor="#488ED3"|Normal[{{cite web |url=http://www.sanger.ac.uk/mouseportal/phenotyping/MBKW/citrobacter-challenge/ |title=Citrobacter infection data for Nsun2 |publisher=Wellcome Trust Sanger Institute}}] |
colspan=2; style="text-align: center;" | All tests and analysis from[{{cite web |url=http://onlinelibrary.wiley.com/doi/10.1111/j.1755-3768.2010.4142.x/abstract |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |location=Acta Opthalmologica 88: 925-7.doi:10.1111/j.1755-3768.2010.4142.x |publisher=Wiley}}][[http://www.sanger.ac.uk/mouseportal/ Mouse Resources Portal], Wellcome Trust Sanger Institute.] |
Model organisms have been used in the study of NSUN2 function. A conditional knockout mouse line, called Nsun2tm1a(EUCOMM)Wtsi[{{cite web |url=http://www.knockoutmouse.org/martsearch/search?query=Nsun2 |title=International Knockout Mouse Consortium}}][{{cite web |url=http://www.informatics.jax.org/searchtool/Search.do?query=MGI:4432654 |title=Mouse Genome Informatics}}] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[{{Cite journal| last1 = Skarnes |first1 =W. C.| doi = 10.1038/nature10163 | last2 = Rosen | first2 = B.| last3 = West | first3 = A. P.| last4 = Koutsourakis | first4 = M.| last5 = Bushell | first5 = W.| last6 = Iyer | first6 = V.| last7 = Mujica | first7 = A. O.| last8 = Thomas | first8 = M.| last9 = Harrow | first9 = J.| last10 = Cox | first10 = T.| last11 = Jackson | first11 = D.| last12 = Severin | first12 = J.| last13 = Biggs | first13 = P.| last14 = Fu | first14 = J.| last15 = Nefedov | first15 = M.| last16 = De Jong | first16 = P. J.| last17 = Stewart | first17 = A. F.| last18 = Bradley | first18 = A. | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–342 | year = 2011 | pmid = 21677750 | pmc =3572410 }}][{{cite web |url=http://www.nature.com/news/2011/110615/full/474262a.html |title=Mouse library set to be knockout |author=Dolgin E |year=June 2011 |location=Nature 474: 262-263. doi:10.1038/474262a}}][{{cite book |title=A mouse for all reasons |author=Collins FS, Rossant J, Wurst W |year=January 2007 |location=Cell 128(1): 9-13. doi:10.1016/j.cell.2006.12.018 PMID 17218247}}]
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[{{cite journal| author=van der Weyden L, White JK, Adams DJ, Logan DW| title=The mouse genetics toolkit: revealing function and mechanism. | journal=Genome Biol | year= 2011 | volume= 12 | issue= 6 | pages= 224 | pmid=21722353 | doi=10.1186/gb-2011-12-6-224 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=21722353 }} ] Twenty eight tests were carried out on mutant mice and fourteen significant abnormalities were observed. Homozygous mutants were subviable and had decreased body weights, length of long bones and decreased circulating glucose levels, numerous abnormal body composition, X-ray imaging, eye morphology and haematology parameters; males also had a decreased grip strength, a short upturned snout, and abnormal indirect calorimetry and plasma chemistry parameters.
In addition, heterozygote mutants displayed premature hair follicle exogen.