type VII secretion system

Cryogenic electron microscopy was used to determine that a complex of two identical subunits made from four proteins forms the structure of the type VII secretion system in Mycobacterium smegmatis.{{cite journal | vauthors = Famelis N, Rivera-Calzada A, Degliesposti G, Wingender M, Mietrach N, Skehel JM, Fernandez-Leiro R, Böttcher B, Schlosser A, Llorca O, Geibel S | display-authors = 6 | title = Architecture of the mycobacterial type VII secretion system | journal = Nature | volume = 576 | issue = 7786 | pages = 321–325 | date = December 2019 | pmid = 31597161 | doi = 10.1038/s41586-019-1633-1 | pmc = 6914368 | bibcode = 2019Natur.576..321F }} T7SS forms a six-sided complex that allows for nearly 165 membrane attachments.{{Cite web |title=Structure of Type VII Secretion System Revealed |url=https://www.cssb-hamburg.de/news_amp_events/articles/2021/structure_of_type_vii_secretion_system_revealed/index_eng.html#lg=gallery-134992&slide=0 |access-date=2022-10-22 |website=www.cssb-hamburg.de |language=en}} This shows how complex the secretion system is. The MDa complex of the Type VII secretion system is found embedded in the inner membrane.

The T7SS structure in Mycobacteria is 28.5 nm in width and 20 nm in height. This secretion system is composed of the following components: inner EccB5, outer EccB5, EccC5, inner EccD5, outer EccD5, EccE5 and MycP5.{{cite journal | vauthors = Bunduc CM, Fahrenkamp D, Wald J, Ummels R, Bitter W, Houben EN, Marlovits TC | title = Structure and dynamics of a mycobacterial type VII secretion system | journal = Nature | volume = 593 | issue = 7859 | pages = 445–448 | date = May 2021 | pmid = 33981042 | pmc = 8131196 | doi = 10.1038/s41586-021-03517-z | bibcode = 2021Natur.593..445B }} These components make the 2.32-MDa complex. This complex is connected to an inner membrane by 165 transmembrane helices. The membrane is composed of a trimer of dimers. The dimers are made up of one copy of MycP5, EccB5, EccC5, EccE5, and two copies of EccD5. The MycP5 structure is what stabilizes the complex. Without the MycP5 complex, EccB5 copies cannot make the stable triangular scaffold. In the membrane EccD5 create barrels that are hypothetically filled with lipids. EccC is the only component of the T7SS that is present in all species that contain a type VII secretion system.

The core machinery of the Type VII secretion system is found in the inner membrane. Once this core machinery is assembled the Type VII secretion system exports alpha helical protein residues using ATP-ase. Type VII secretion systems use proteins from the ESX-1 system of secretion proteins.{{cite journal | vauthors = Abdallah AM, Gey van Pittius NC, Champion PA, Cox J, Luirink J, Vandenbroucke-Grauls CM, Appelmelk BJ, Bitter W | display-authors = 6 | title = Type VII secretion--mycobacteria show the way | journal = Nature Reviews. Microbiology | volume = 5 | issue = 11 | pages = 883–891 | date = November 2007 | pmid = 17922044 | doi = 10.1038/nrmicro1773 | s2cid = 11307957 }} T7SS uses unique proteins as compared to other secretion systems.

Secretion systems are commonly found in gram-positive bacteria and Mycobacterium. There is also a system referred to as a T7SS in gram negative bacteria.

In gram negative bacteria a Type VII ‘like" secretion system has been observed. It is known as the chaperone-usher fimbriae. This system helps gram negative bacteria colonize, form biofilms, and causes an increase in pathogenicity in the bacteria that utilize it. These systems are observable when genes for an Fimbrial usher protein (which is integral to the formation of a pilus in gram negative bacteria), a Chaperone (protein), and the building blocks of fimbriae are found together.{{cite journal | vauthors = Busch A, Waksman G | title = Chaperone-usher pathways: diversity and pilus assembly mechanism | journal = Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences | volume = 367 | issue = 1592 | pages = 1112–1122 | date = April 2012 | pmid = 22411982 | pmc = 3297437 | doi = 10.1098/rstb.2011.0206 }}

The Type VII secretion system, however, was first observed in firmicutes and actinobacteria, specifically Mycobacterium tuberculosis.{{cite journal | vauthors = Spencer BL, Doran KS | title = Evolving understanding of the type VII secretion system in Gram-positive bacteria | journal = PLOS Pathogens | volume = 18 | issue = 7 | pages = e1010680 | date = July 2022 | pmid = 35901012 | pmc = 9333272 | doi = 10.1371/journal.ppat.1010680 | doi-access = free }}{{cite journal | vauthors = Tran HR, Grebenc DW, Klein TA, Whitney JC | title = Bacterial type VII secretion: An important player in host-microbe and microbe-microbe interactions | journal = Molecular Microbiology | volume = 115 | issue = 3 | pages = 478–489 | date = March 2021 | pmid = 33410158 | doi = 10.1111/mmi.14680 | s2cid = 230817692 | doi-access = free }} The type VII secretion system plays an important role in interbacterial competition, nutrient acquisition, and virulence in Firmicutes (which are spore-forming bacteria). This type of secretion system has also been observed to play a role in the virulence and cytotoxicity of Streptococcus species.{{cite journal | vauthors = Spencer BL, Tak U, Mendonça JC, Nagao PE, Niederweis M, Doran KS | title = A type VII secretion system in Group B Streptococcus mediates cytotoxicity and virulence | journal = PLOS Pathogens | volume = 17 | issue = 12 | pages = e1010121 | date = December 2021 | pmid = 34871327 | pmc = 8675928 | doi = 10.1371/journal.ppat.1010121 | doi-access = free }}

This system uses different proteins in order to function in varying species. The system alters itself and produces variants within each new species. These system variants are identified based on EssC- C terminus and other associated effectors. Variants have been observed in the following species: 4 variants in Group B streptococcus and staphylococcus aureus, and 7 variants within Listeria monocytogenes. This type of secretion system also provides essential cell functions pathways with which to proceed. Mycobacteria have a cell membranes that are impenetrable, T7SS allow for substrates to pass through, making the Type VII Secretion system (also known as ESX) essential for mycobacterial growth and virulence.

T7SS plays a role in the virulence of mycobacterium. Disruption in the genes that encode T7SS called the RD1 locus results in the loss of function of secretion apparatus.{{cite journal | vauthors = Lewis KN, Liao R, Guinn KM, Hickey MJ, Smith S, Behr MA, Sherman DR | title = Deletion of RD1 from Mycobacterium tuberculosis mimics bacille Calmette-Guérin attenuation | journal = The Journal of Infectious Diseases | volume = 187 | issue = 1 | pages = 117–23 | date = January 2003 | pmid = 12508154 | pmc = 1458498 | doi = 10.1086/345862 }} The genes necessary for ESX-1 transport have also been found outside of the RD1 locus.{{cite journal | vauthors = Kennedy GM, Hooley GC, Champion MM, Mba Medie F, Champion PA | title = A novel ESX-1 locus reveals that surface-associated ESX-1 substrates mediate virulence in Mycobacterium marinum | journal = Journal of Bacteriology | volume = 196 | issue = 10 | pages = 1877–1888 | date = May 2014 | pmid = 24610712 | pmc = 4011007 | doi = 10.1128/JB.01502-14 }} This means that multiple genes are required for protein transport and disruption of these genes results in the loss of function in the secretion systems. The ESX-1 system secretes polypeptides which causes a lytic effect though the specific polypeptide is not known. The extended RD1 (extRD1) region expresses membrane lytic activity in mycobacteria. The extRD1 genes are necessary for haemolysis activity.{{cite journal | vauthors = Tran HR, Grebenc DW, Klein TA, Whitney JC | title = Bacterial type VII secretion: An important player in host-microbe and microbe-microbe interactions | journal = Molecular Microbiology | volume = 115 | issue = 3 | pages = 478–489 | date = March 2021 | pmid = 33410158 | doi = 10.1111/mmi.14680 | s2cid = 230817692 | doi-access = free }} Genetic changes to the ESX-1 system result in the loss of a secretion activity. In infection models this leads to a loss of virulence.

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Category:Secretion

Category:Bacteriology