2025 U.S. Open Cup#First round

{{main|2025 U.S. Open Cup qualification}}

• Bold denotes team is still active in the tournament.
• {{anchor|Money}} $: Winner of $50,000 bonus for advancing the furthest in the competition from their respective divisions.{{Cite web |date=March 18, 2025 |title=Lamar Hunt U.S. Open Cup Tournament Kicks Off Today; Increased Investment Leads to Record Purse of $1 Million |url=https://www.ussoccer.com/stories/2025/03/us-open-cup-prize-money-record-increased-investment |access-date=March 18, 2025 |publisher=United States Soccer Federation |language=en}}
• {{anchor|Runner-up}} $$: Winner of $250,000 for being the runner-up in the competition.
• {{anchor|Champion}} $$$: Winner of $600,000 for winning the competition.

{{notelist}}

{{refbegin}}

{{reflist|group=lower-roman}}

{{refend}}

{{Location map+ |USA |width=1300 |float=center |caption=Locations of teams in the tournament proper.

{{hlist

| 8px MLS

| 8px USLC

| 8px Division 3 (MLSNP/USL1)

| 8px Open Division (USL2/NPSL)

| 8px Open Division (USASA/USSSA)

| 8px Multiple Teams

}}

Metro areas with multiple teams:

• Boston: New England Revolution (MLS), CD Faialense (BSSL)
• Chattanooga: Chattanooga FC (MLSNP), Chattanooga Red Wolves SC (USL1)
• Charlotte: Charlotte FC (MLS), Charlotte Independence (USL1)
• Dallas: FC Dallas (MLS), Foro SC (UPSL)
• Denver: Flatirons FC (USL2), Harpos FC (CPL)
• Los Angeles: Orange County SC (USLC), Los Angeles FC 2 (MLSNP), Ventura County FC (MLSNP), Laguna United FC (UPSL), Ventura County Fusion (USL2)
• Louisville: Louisville City FC (USLC), Southern Indiana FC (UPSL)
• Miami: Inter Miami CF II (MLSNP), Miami United FC (UPSL), Miami FC (USLC)
• Naples: FC Naples (USL1), Naples United FC (NPSL)
• New York City: New York City FC (MLS), New York Red Bulls (MLS), Westchester SC (USL1), FC Motown (NPSL), New York Pancyprian-Freedoms (EPSL), NY Renegades FC (UPSL)
• Philadelphia: Philadelphia Union (MLS), West Chester United SC (USLPA)
• Phoenix: Phoenix Rising FC (USLC), FC Arizona (NPSL)
• San Francisco Bay Area: San Jose Earthquakes (MLS), Oakland Roots SC (USLC), El Farolito SC (NPSL), International San Francisco (SFSFL)
• Seattle: Ballard FC (USL2), Washington Athletic Club (SRATS)
• Tulsa: FC Tulsa (USLC), Tulsa Athletic (UPSL)
• Washington D.C.: D.C. United (MLS), Loudoun United FC (USLC), Virginia Dream FC (VSSL)

|places=

{{Location map~ |USA |lat=30.265176604066934 |long=-97.73023842579566 |label=Austin FC |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=41.8750197162963 |long=-87.6343883516116 |label=Chicago Fire FC |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=29.752255671778688 |long=-95.35222014719191 |label=Houston Dynamo FC |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=45.15825575667031 |long=-93.2300211108754 |label=Minnesota United FC |label_size=80 |position=right |mark=Red pog.svg}}

{{Location map~ |USA |lat=36.15958280552819 |long=-86.77559003465385 |label=Nashville SC |label_size=80 |position=bottom |mark=Red pog.svg}}

{{Location map~ |USA |lat=28.5306116521087 |long=-81.37439500176559 |label=Orlando City SC |label_size=80 |position=top |mark=Red pog.svg}}

{{Location map~ |USA |lat=45.51421505933525 |long=-122.6873568042184 |label={{nowrap|Portland Timbers}} |label_size=80 |position=bottom |mark=Red pog.svg}}

{{Location map~ |USA |lat=38.62514507656682 |long=-90.19762430849141 |label={{nowrap|St. Louis City SC}} |label_size=80 |position=bottom |mark=Red pog.svg}}

{{Location map~ |USA |lat=33.51860982216501 |long=-86.81046673158751 |label=Birmingham Legion FC |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=32.79515706257136 |long=-79.90296204549213 |label={{nowrap|Charleston Battery}} |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=38.83390079067579 |long=-104.82150063775298 |label=Colorado Springs Switchbacks FC |label_size=80 |position=bottom |mark=Orange pog.svg}}

{{Location map~ |USA |lat=42.33369889288626 |long=-83.05174942285727 |label={{nowrap|Detroit City FC}} |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=31.761954479179543 |long=-106.48492429902919 |label={{nowrap|El Paso Locomotive FC}} |label_size=80 |position=top |mark=Orange pog.svg}}

{{Location map~ |USA |lat=41.75456328851781 |long=-72.66092765396853 |label={{nowrap|Hartford Athletic}} |label_size=80 |position=bottom |mark=Orange pog.svg}}

{{Location map~ |USA |lat=39.7684897971886 |long=-86.15803260219954 |label=Indy Eleven |label_size=80 |position=top |mark=Orange pog.svg}}

{{Location map~ |USA |lat=36.16922858479163 |long=-115.14065221352614 |label={{nowrap|Las Vegas Lights FC}} |label_size=80 |position=top |mark=Orange pog.svg}}

{{Location map~ |USA |lat=38.029722 |long=-84.494722 |label=Lexington SC |label_size=80 |position=bottom |mark=Orange pog.svg}}

{{Location map~ |USA |lat=36.61490418002709 |long=-121.82204912721032 |label=Monterey Bay FC |label_size=80 |position=bottom |mark=Orange pog.svg}}

{{Location map~ |USA |lat=35.08418344509937 |long=-106.65086953523182 |label={{nowrap|New Mexico United}} |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=35.79143485820475 |long=-78.78113885194223 |label={{nowrap|North Carolina FC}} |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=40.441176138776044 |long=-80.00496469288136 |label=Pittsburgh Riverhounds SC |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=41.92508407650724 |long=-71.53855531534383 |label={{nowrap|Rhode Island FC}} |label_size=80 |position=right |mark=Orange pog.svg}}

{{Location map~ |USA |lat=38.57399130107782 |long=-121.49265172177475 |label=Sacramento Republic FC |label_size=80 |position=top |mark=Orange pog.svg}}

{{Location map~ |USA |lat=29.424126971749406 |long=-98.49371772852207 |label={{no wrap|San Antonio FC}} |label_size=80 |position=bottom |mark=Orange pog.svg}}

{{Location map~ |USA |lat=27.76760384836234 |long=-82.64016609082519 |label=Tampa Bay Rowdies |label_size=80 |position=left |mark=Orange pog.svg}}

{{Location map~ |USA |lat=34.699087747704695 |long=-118.13468336746286 |label={{nowrap|AV Alta FC}} |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=35.95980459435879 |long=-80.01125324539136 |label={{nowrap|Carolina Core FC}} |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=39.96185627161333 |long=-82.99151600982043 |label={{nowrap|Columbus Crew 2}} |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=39.10184082253231 |long=-84.50871318110119 |label={{nowrap|FC Cincinnati 2}} |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=43.07305651659342 |long=-89.40122671893994 |label=Forward Madison FC |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=34.85263694973682 |long=-82.39409386584747 |label={{nowrap|Greenville Triumph SC}} |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=35.9617 |long=-83.9232 |label={{nowrap|One Knoxville SC}} |label_size=80 |position=left |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=43.657891 |long=-70.276734 |label=Portland Hearts of Pine |label_size=80 |position=top |mark = Yellow pog.svg}}

{{Location map~ |USA |lat=40.51329006810991 |long=-112.03159509942049 |label={{nowrap|Real Monarchs}} |label_size=80 |position=left |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=37.54069231180277 |long=-77.4360245445324 |label=Richmond Kickers |label_size=80 |position=left |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=32.44916936581426 |long=-81.78225169408233 |label=South Georgia Tormenta FC |label_size=80 |position=bottom |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=47.66824616017965 |long=-117.4193797 |label={{nowrap|Spokane Velocity FC}} |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=39.09616550925763 |long=-94.60302726716235 |label={{nowrap|Sporting Kansas City II}} |label_size=80 |position=top |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=47.24487069316747 |long=-122.41021850032894 |label={{nowrap|Tacoma Defiance}} |label_size=80 |position=bottom |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=33.6340901537769 |long=-96.6073594738203 |label={{nowrap|Texoma FC}} |label_size=80 |position=right |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=41.2565273110203 |long=-95.93449814923649 |label={{nowrap|Union Omaha}} |label_size=80 |position=left |mark=Yellow pog.svg}}

{{Location map~ |USA |lat=36.21695753433791 |long=-81.67401183294726 |label=Appalachian FC |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=35.595623821757165 |long=-82.5537324769243 |label={{nowrap|Asheville City SC}} |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=27.794744461241248 |long=-97.41837310047289 |label=Corpus Christi FC |label_size=80 |position=right |mark=Green pog.svg}}

{{Location map~ |USA |lat=41.58681948015526 |long=-93.62491357165688 |label=Des Moines Menace |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=46.78694444444444 |long=-92.09805555555556 |label=Duluth FC |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=34.745061010941605 |long=-92.28531446401794 |label=Little Rock Rangers |label_size=80 |position=top |mark=Green pog.svg}}

{{Location map~ |USA |lat=40.72682863264211 |long= -73.59946233209006 |label={{nowrap|Long Island Rough Riders}} |label_size=80 |position=right |mark=Green pog.svg}}

{{Location map~ |USA |lat=42.74354188236711 |long= -73.82687107474591 |label=New York Shockers |label_size=80 |position=left |mark=Green pog.svg}}

{{Location map~ |USA |lat=27.335954807914565 |long=-82.53148588966563 |label=Sarasota Paradise |label_size=80 |position=right |mark=Green pog.svg}}

{{Location map~ |USA |lat=28.082693413723977 |long=-80.60722272718913 |label={{nowrap|Harbor City FC}} |label_size=80 |position=right |mark=Blue pog.svg}}

{{Location map~ |USA |lat=40.80601416107224 |long=-74.11028841043976 |label={{nowrap|New Jersey Alliance FC}} |label_size=80 |position=left |mark=Blue pog.svg}}

{{Location map~ |USA |lat=34.00299982312376 |long=-81.04331775003075 |label={{nowrap|Soda City FC}} |label_size=80 |position=bottom |mark=Blue pog.svg}}

{{Location map~ |USA |lat=42.35383951856906 |long=-71.04556842617212 |label=Boston |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=35.045719432756805 |long=-85.31043593018825 |label=Chattanooga |label_size=80 |position=bottom |mark=Purple pog.svg}}

{{Location map~ |USA |lat=35.22721552837581 |long=-80.84309227188619 |label=Charlotte |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=32.78130937397232 |long=-96.79130359367997 |label=Dallas |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=39.74090021889915 |long=-104.99140826083092 |label=Denver |label_size=80 |position=top |mark=Purple pog.svg}}

{{Location map~ |USA |lat=34.056 |long=-118.237 |label=Los Angeles |label_size=80 |position=bottom |mark=Purple pog.svg}}

{{Location map~ |USA |lat=38.24795098889253 |long=-85.75497942402936 |label=Louisville |label_size=80 |position=top |mark=Purple pog.svg}}

{{Location map~ |USA |lat=25.761609871804747 |long=-80.18725238426836 |label=Miami |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=26.141352726912956 |long=-81.79076978264092 |label=Naples |label_size=80 |position=bottom |mark=Purple pog.svg}}

{{Location map~ |USA |lat=40.71142538788587 |long=-74.01539826475124 |label=NYC |label_size=80 |position=bottom |mark=Purple pog.svg}}

{{Location map~ |USA |lat=39.83289156958042 |long=-75.37839461894184 |label=Philadelphia |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=33.44704678060311 |long=-112.05206650407428 |label=Phoenix |label_size=80 |position=right |mark=Purple pog.svg}}

{{Location map~ |USA |lat=37.610045 |long=-122.22312 |label=San Francisco Bay Area |label_size=80 |position=left |mark=Purple pog.svg}}

{{Location map~ |USA |lat=47.608134061117376 |long=-122.33774984630327 |label=Seattle |label_size=80 |position=top |mark=Purple pog.svg}}

{{Location map~ |USA |lat=36.15397991781358 |long=-95.99277897597904 |label=Tulsa |label_size=80 |position=top |mark=Purple pog.svg}}

{{Location map~ |USA |lat=38.90494757429535 |long=-77.026939015084 |label=Washington D.C. |label_size=80 |position=right |mark=Purple pog.svg}}

}}

A total of 36 states and D.C. were represented by clubs in the U.S. Open Cup this year.

26 teams made their Open Cup debuts in the 2025 tournament.

• MLSNP: Columbus Crew 2, FC Cincinnati 2, Los Angeles FC 2, Inter Miami CF II, Real Monarchs, Sporting Kansas City II
• USL1: AV Alta FC, FC Naples, Portland Hearts of Pine, Texoma FC, Westchester SC
• NPSL: Naples United FC, New York Shockers
• USL2: Corpus Christi FC, Flatirons FC, Little Rock Rangers, Sarasota Paradise
• Local: CD Faialense, Harbour City FC, Laguna United FC, New Jersey Alliance FC, NY Renegades FC, Soda City FC, Southern Indiana FC, Virginia Dream FC, Washington Athletic Club

The first three rounds of the tournament bracket are shown below, with bold denoting the winners of each match.{{Cite web |date=February 5, 2025 |title=2025 Lamar Hunt US Open Cup Schedule, Results |url=https://thecup.us/2025/02/05/2025-lamar-hunt-us-open-cup-schedule-results/ |access-date=February 23, 2025 |website=thecup.us |language=en-US}}

{{2NTeam-NTeamBracket

| rounds = 3

| brackets = 16

| RD1-teams = 4

| boldwinner = y

| seed-width = 50

| team-width = 205

| score-width = 30

| RD1 = First round
March 18–21

| RD2 = Second round
April 1–2

| RD3 = Third round
April 15–16

| RD1-seed01 = MLSNP

| RD1-team01 = {{flagicon|Ohio}} Columbus Crew 2

| RD1-score01 = 4

| RD1-seed02 = UPSL

| RD1-team02 = {{flagicon|New York}} NY Renegades FC

| RD1-score02 = 1

| RD1-seed03 = USLC

| RD1-team03 = {{flagicon|Kentucky}} Lexington SC

| RD1-score03 = 5

| RD1-seed04 = UPSL

| RD1-team04 = {{flagicon|Indiana}} Southern Indiana FC

| RD1-score04 = 0

| RD1-seed05 = USL1

| RD1-team05 = {{flagicon|Georgia (U.S. state)}} South Georgia Tormenta FC

| RD1-score05 = 3

| RD1-seed06 = UPSL

| RD1-team06 = {{flagicon|Florida}} Harbor City FC

| RD1-score06 = 0

| RD1-seed07 = USL1

| RD1-team07 = {{flagicon|Texas}} Texoma FC

| RD1-score07 = 1

| RD1-seed08 = UPSL

| RD1-team08 = {{flagicon|Texas}} Foro SC

| RD1-score08 = 2

| RD1-seed09 = MLSNP

| RD1-team09 = {{flagicon|Florida}} Inter Miami CF II

| RD1-score09 = 4

| RD1-seed10 = UPSL

| RD1-team10 = {{flagicon|Florida}} Miami United FC

| RD1-score10 = 2

| RD1-seed11 = USLC

| RD1-team11 = {{flagicon|Florida}} Miami FC

| RD1-score11 = 4

| RD1-seed12 = NPSL

| RD1-team12 = {{flagicon|Florida}} Naples United FC

| RD1-score12 = 1

| RD1-seed13 = USL2

| RD1-team13 = {{flagicon|New York}} Long Island Rough Riders

| RD1-score13 = 2

| RD1-seed14 = USL1

| RD1-team14 = {{flagicon|North Carolina}} Charlotte Independence

| RD1-score14 = 3

| RD1-seed15 = MLSNP

| RD1-team15 = {{flagicon|North Carolina}} Carolina Core FC

| RD1-score15 = 4

| RD1-seed16 = UPSL

| RD1-team16 = {{flagicon|South Carolina}} Soda City FC

| RD1-score16 = 2

| RD1-seed17 = USLC

| RD1-team17 = {{flagicon|California}} Monterey Bay FC

| RD1-score17 = 4

| RD1-seed18 = SFSFL

| RD1-team18 = {{flagicon|California}} International San Francisco

| RD1-score18 = 0

| RD1-seed19 = MLSNP

| RD1-team19 = {{flagicon|Utah}} Real Monarchs

| RD1-score19 = 1

| RD1-seed20 = NPSL

| RD1-team20 = {{flagicon|California}} El Farolito SC

| RD1-score20 = 3

| RD1-seed21 = USL1

| RD1-team21 = {{flagicon|California}} AV Alta FC

| RD1-score21 = 3

| RD1-seed22 = USL2

| RD1-team22 = {{flagicon|California}} Ventura County Fusion

| RD1-score22 = 1

| RD1-seed23 = MLSNP

| RD1-team23 = {{flagicon|California}} Los Angeles FC 2

| RD1-score23 = 7

| RD1-seed24 = NPSL

| RD1-team24 = {{flagicon|Arizona}} FC Arizona

| RD1-score24 = 1

| RD1-seed25 = USLPA

| RD1-team25 = {{flagicon|Pennsylvania}} West Chester United SC

| RD1-score25 = 2

| RD1-seed26 = USLC

| RD1-team26 = {{flagicon|Virginia}} Loudoun United FC

| RD1-score26 = 3

| RD1-seed27 = USL1

| RD1-team27 = {{flagicon|Virginia}} Richmond Kickers

| RD1-score27 = 1

| RD1-seed28 = VSSL

| RD1-team28 = {{flagicon|Virginia}} Virginia Dream FC

| RD1-score28 = 3

| RD1-seed29 = USL1

| RD1-team29 = {{flagicon|Maine}} Portland Hearts of Pine

| RD1-score29 = 4

| RD1-seed30 = BSSL

| RD1-team30 = {{flagicon|Massachusetts}} CD Faialense

| RD1-score30 = 0

| RD1-seed31 = USLC

| RD1-team31 = {{flagicon|Connecticut}} Hartford Athletic

| RD1-score31 = 3

| RD1-seed32 = NPSL

| RD1-team32 = {{flagicon|New York}} New York Shockers

| RD1-score32 = 0

| RD1-seed33 = NPSL

| RD1-team33 = {{flagicon|New Jersey}} FC Motown

| RD1-score33 = 0

| RD1-seed34 = USL1

| RD1-team34 = {{flagicon|New York}} Westchester SC

| RD1-score34 = 1

| RD1-seed35 = MLSNP

| RD1-team35 = {{flagicon|Ohio}} FC Cincinnati 2

| RD1-score35 = 0 (4)

| RD1-seed36 = EPSL

| RD1-team36 = {{flagicon|New York}} New York Pancyprian-Freedoms

| RD1-score36 = 0 (5)

| RD1-seed37 = MLSNP

| RD1-team37 = {{flagicon|Tennessee}} Chattanooga FC

| RD1-score37 = 1

| RD1-seed38 = USL2

| RD1-team38 = {{flagicon|Texas}} Corpus Christi FC

| RD1-score38 = 0

| RD1-seed39 = UPSL

| RD1-team39 = {{flagicon|New Jersey}} New Jersey Alliance FC

| RD1-score39 = 2 (2)

| RD1-seed40 = USL1

| RD1-team40 = {{flagicon|Tennessee}} Chattanooga Red Wolves SC

| RD1-score40 = 2 (4)

| RD1-seed41 = USL2

| RD1-team41 = {{flagicon|North Carolina}} Asheville City SC

| RD1-score41 = 0 (3)

| RD1-seed42 = USL1

| RD1-team42 = {{flagicon|South Carolina}} Greenville Triumph SC

| RD1-score42 = 0 (4)

| RD1-seed43 = NPSL

| RD1-team43 = {{flagicon|North Carolina}} Appalachian FC

| RD1-score43 = 0

| RD1-seed44 = USL1

| RD1-team44 = {{flagicon|Tennessee}} One Knoxville SC

| RD1-score44 = 3

| RD1-seed45 = USL2

| RD1-team45 = {{flagicon|Florida}} Sarasota Paradise

| RD1-score45 = 1

| RD1-seed46 = USL1

| RD1-team46 = {{flagicon|Florida}} FC Naples

| RD1-score46 = 2

| RD1-seed47 = USL2

| RD1-team47 = {{flagicon|Arkansas}} Little Rock Rangers

| RD1-score47 = 1

| RD1-seed48 = USLC

| RD1-team48 = {{flagicon|Alabama}} Birmingham Legion FC

| RD1-score48 = 0

| RD1-seed49 = USL1

| RD1-team49 = {{flagicon|Wisconsin}} Forward Madison FC

| RD1-score49 = 5

| RD1-seed50 = NPSL

| RD1-team50 = {{flagicon|Minnesota}} Duluth FC

| RD1-score50 = 1

| RD1-seed51 = UPSL

| RD1-team51 = {{flagicon|Oklahoma}} Tulsa Athletic

| RD1-score51 = 0

| RD1-seed52 = USLC

| RD1-team52 = {{flagicon|Oklahoma}} FC Tulsa

| RD1-score52 = 1

| RD1-seed53 = MLSNP

| RD1-team53 = {{flagicon|Missouri}} Sporting Kansas City II

| RD1-score53 = 1

| RD1-seed54 = USL2

| RD1-team54 = {{flagicon|Iowa}} Des Moines Menace

| RD1-score54 = 2

| RD1-seed55 = USL2

| RD1-team55 = {{flagicon|Colorado}} Flatirons FC

| RD1-score55 = 1

| RD1-seed56 = USL1

| RD1-team56 = {{flagicon|Nebraska}} Union Omaha

| RD1-score56 = 2

| RD1-seed57 = MLSNP

| RD1-team57 = {{flagicon|Washington}} Tacoma Defiance

| RD1-score57 = 3

| RD1-seed58 = SRATS

| RD1-team58 = {{flagicon|Washington}} Washington Athletic Club

| RD1-score58 = 1

| RD1-seed59 = USL2

| RD1-team59 = {{flagicon|Washington}} Ballard FC

| RD1-score59 = 0

| RD1-seed60 = USL1

| RD1-team60 = {{flagicon|Washington}} Spokane Velocity FC

| RD1-score60 = 1

| RD1-seed61 = MLSNP

| RD1-team61 = {{flagicon|California}} Ventura County FC

| RD1-score61 = 7

| RD1-seed62 = UPSL

| RD1-team62 = {{flagicon|California}} Laguna United FC

| RD1-score62 = 0

| RD1-seed63 = USLC

| RD1-team63 = {{flagicon|Texas}} El Paso Locomotive FC

| RD1-score63 = 5

| RD1-seed64 = CPL

| RD1-team64 = {{flagicon|Colorado}} Harpos FC

| RD1-score64 = 1

| RD2-seed01 = MLSNP

| RD2-team01 = {{flagicon|Ohio}} Columbus Crew 2

| RD2-score01 = 3

| RD2-seed02 = USLC

| RD2-team02 = {{flagicon|Kentucky}} Lexington SC

| RD2-score02 = 0

| RD2-seed03 = USL1

| RD2-team03 = {{flagicon|Georgia (U.S. state)}} South Georgia Tormenta FC

| RD2-score03 = 3

| RD2-seed04 = UPSL

| RD2-team04 = {{flagicon|Texas}} Foro SC

| RD2-score04 = 2

| RD2-seed05 = MLSNP

| RD2-team05 = {{flagicon|Florida}} Inter Miami CF II

| RD2-score05 = 0

| RD2-seed06 = USLC

| RD2-team06 = {{flagicon|Florida}} Miami FC

| RD2-score06 = 1

| RD2-seed07 = USL1

| RD2-team07 = {{flagicon|North Carolina}} Charlotte Independence

| RD2-score07 = 2

| RD2-seed08 = MLSNP

| RD2-team08 = {{flagicon|North Carolina}} Carolina Core FC

| RD2-score08 = 1

| RD2-seed09 = USLC

| RD2-team09 = {{flagicon|California}} Monterey Bay FC

| RD2-score09 = 1

| RD2-seed10 = NPSL

| RD2-team10 = {{flagicon|California}} El Farolito SC

| RD2-score10 = 2

| RD2-seed11 = USL1

| RD2-team11 = {{flagicon|California}} AV Alta FC

| RD2-score11 = 2

| RD2-seed12 = MLSNP

| RD2-team12 = {{flagicon|California}} Los Angeles FC 2

| RD2-score12 = 1

| RD2-seed13 = USLC

| RD2-team13 = {{flagicon|Virginia}} Loudoun United FC

| RD2-score13 =4

| RD2-seed14 = VSSL

| RD2-team14 = {{flagicon|Virginia}} Virginia Dream FC

| RD2-score14 =2

| RD2-seed15 = USL1

| RD2-team15 = {{flagicon|Maine}} Portland Hearts of Pine

| RD2-score15 = 1 (4)

| RD2-seed16 = USLC

| RD2-team16 = {{flagicon|Connecticut}}

Hartford Athletic

| RD2-score16 = 1 (2)

| RD2-seed17 = USL1

| RD2-team17 = {{flagicon|New York}} Westchester SC

| RD2-score17 =3

| RD2-seed18 = EPSL

| RD2-team18 = {{flagicon|New York}} New York Pancyprian-Freedoms

| RD2-score18 =2

| RD2-seed19 = MLSNP

| RD2-team19 = {{flagicon|Tennessee}} Chattanooga FC

| RD2-score19 =1 (3)

| RD2-seed20 = USL1

| RD2-team20 = {{flagicon|Tennessee}} Chattanooga Red Wolves SC

| RD2-score20 =1 (4)

| RD2-seed21 = USL1

| RD2-team21 = {{flagicon|South Carolina}} Greenville Triumph SC

| RD2-score21 =1

| RD2-seed22 = USL1

| RD2-team22 = {{flagicon|Tennessee}} One Knoxville SC

| RD2-score22 =3

| RD2-seed23 = USL1

| RD2-team23 = {{flagicon|Florida}} FC Naples

| RD2-score23 =3

| RD2-seed24 = USL2

| RD2-team24 = {{flagicon|Arkansas}} Little Rock Rangers

| RD2-score24 =0

| RD2-seed25 = USL1

| RD2-team25 = {{flagicon|Wisconsin}} Forward Madison FC

| RD2-score25 =1

| RD2-seed26 = USLC

| RD2-team26 = {{flagicon|Oklahoma}} FC Tulsa

| RD2-score26 =3

| RD2-seed27 = USL2

| RD2-team27 = {{flagicon|Iowa}} Des Moines Menace

| RD2-score27 =1

| RD2-seed28 = USL1

| RD2-team28 = {{flagicon|Nebraska}} Union Omaha

| RD2-score28 =2

| RD2-seed29 = MLSNP

| RD2-team29 =

{{flagicon|Washington}} Tacoma Defiance

| RD2-score29 =2

| RD2-seed30 = USL1

| RD2-team30 = {{flagicon|Washington}} Spokane Velocity FC

| RD2-score30 = 1

| RD2-seed31 = MLSNP

| RD2-team31 = {{flagicon|California}} Ventura County FC

| RD2-score31 = 0

| RD2-seed32 = USLC

| RD2-team32 = {{flagicon|Texas}} El Paso Locomotive

| RD2-score32 =3

| RD3-seed01 =MLSNP

| RD3-team01 ={{flagicon|Ohio}} Columbus Crew 2

| RD3-score01 =0

| RD3-seed02 =USLC

| RD3-team02 ={{flagicon|Pennsylvania}} Pittsburgh Riverhounds SC

| RD3-score02 =1

| RD3-seed03 =USLC

| RD3-team03 ={{flagicon|South Carolina}} Charleston Battery

| RD3-score03 =4

| RD3-seed04 =USL1

| RD3-team04 ={{flagicon|Georgia (U.S. state)}} South Georgia Tormenta FC

| RD3-score04 =0

| RD3-seed05 =USLC

| RD3-team05 ={{flagicon|Indiana}} Indy Eleven

| RD3-score05 =1

| RD3-seed06 =USLC

| RD3-team06 ={{flagicon|Florida}} Miami FC

| RD3-score06 =0

| RD3-seed07 =USL1

| RD3-team07 ={{flagicon|North Carolina}} Charlotte Independence

| RD3-score07 =1

| RD3-seed08 =USLC

| RD3-team08 ={{flagicon|North Carolina}} North Carolina FC

| RD3-score08 =3

| RD3-seed09 =USLC

| RD3-team09 ={{flagicon|California}} Sacramento Republic FC

| RD3-score09 =1

| RD3-seed10 =NPSL

| RD3-team10 ={{flagicon|California}} El Farolito SC

| RD3-score10 =0

| RD3-seed11 =USL1

| RD3-team11 ={{flagicon|California}} AV Alta FC

| RD3-score11 =2 (4)

| RD3-seed12 =USLC

| RD3-team12 ={{flagicon|California}} Orange County SC

| RD3-score12 =2 (2)

| RD3-seed13 =USLC

| RD3-team13 ={{flagicon|Kentucky}} Louisville City FC

| RD3-score13 =2

| RD3-seed14 =USLC

| RD3-team14 ={{flagicon|Virginia}} Loudoun United FC

| RD3-score14 =1

| RD3-seed15 =USL1

| RD3-team15 ={{flagicon|Maine}} Portland Hearts of Pine

| RD3-score15 =1

| RD3-seed16 =USLC

| RD3-team16 ={{flagicon|Rhode Island}} Rhode Island FC

| RD3-score16 =2

| RD3-seed17 =USLC

| RD3-team17 ={{flagicon|Michigan}} Detroit City FC

| RD3-score17 =3

| RD3-seed18 =USL1

| RD3-team18 ={{flagicon|New York}} Westchester SC

| RD3-score18 =1

| RD3-seed19 =USLC

| RD3-team19 ={{flagicon|Nevada}} Las Vegas Lights FC

| RD3-score19 =2 (3)

| RD3-seed20 =USL1

| RD3-team20 ={{flagicon|Tennessee}} Chattanooga Red Wolves SC

| RD3-score20 =2 (4)

| RD3-seed21 =USLC

| RD3-team21 ={{flagicon|Colorado}} Colorado Springs Switchbacks FC

| RD3-score21 =3

| RD3-seed22 =USL1

| RD3-team22 ={{flagicon|Tennessee}} One Knoxville SC

| RD3-score22 =2

| RD3-seed23 =USL1

| RD3-team23 ={{flagicon|Florida}} FC Naples

| RD3-score23 =1 (8)

| RD3-seed24 =USLC

| RD3-team24 ={{flagicon|Florida}} Tampa Bay Rowdies

| RD3-score24 =1 (9)

| RD3-seed25 =USLC

| RD3-team25 ={{flagicon|Oklahoma}} FC Tulsa

| RD3-score25 =1 (2)

| RD3-seed26 =USLC

| RD3-team26 ={{flagicon|Arizona}} Phoenix Rising FC

| RD3-score26 =1 (4)

| RD3-seed27 =USL1

| RD3-team27 ={{flagicon|Nebraska}} Union Omaha

| RD3-score27 =1

| RD3-seed28 =USLC

| RD3-team28 ={{flagicon|Texas}} San Antonio FC

| RD3-score28 =0

| RD3-seed29 =MLSNP

| RD3-team29 ={{flagicon|Washington}} Tacoma Defiance

| RD3-score29 =2

| RD3-seed30 =USLC

| RD3-team30 ={{flagicon|California}} Oakland Roots SC

| RD3-score30 =1

| RD3-seed31 =USLC

| RD3-team31 ={{flagicon|New Mexico}} New Mexico United

| RD3-score31 =2 (1)

| RD3-seed32 =USLC

| RD3-team32 ={{flagicon|Texas}} El Paso Locomotive

| RD3-score32 =2 (4)

}}

Host team listed first

Bold = winner

The complete draw for the first round, including match dates and times, was announced on February 6, 2025.{{Cite web |date=February 6, 2025 |title=Draw for 2025 US Open Cup revealed, 110th tournament begins March 18 |url=https://thecup.us/2025/02/06/draw-for-2025-us-open-cup-revealed-110th-tournament-begins-march-18/ |access-date=February 23, 2025 |website=TheCup.us}}

{{football box collapsible

|date = March 18

|time = 6:30 p.m. EDT

|team1 = Hartford Athletic (USLC) {{flagicon|Connecticut}}

|score = 3–0

|report = https://www.espn.com/soccer/match/_/gameId/732258/new-york-shockers-hartford-athletic

|team2 = {{flagicon|New York}} New York Shockers (NPSL)

|goals1 =

• Obalola {{goal|12}}
• Dieng {{goal|38}}
• Ngalina {{goal|87}}

|goals2 =

|stadium = Trinity Health Stadium

|location = Hartford, Connecticut

|attendance = 2,287

|referee = Johnathan Luk

}}

{{football box collapsible

|date = March 18

|time = 7:00 p.m. EDT

|team1 = Sarasota Paradise (USL2) {{flagicon|Florida}}

|score = 1–2

|report = https://www.espn.com/soccer/match/_/gameId/732260/fc-naples-sarasota-paradise

|team2 = {{flagicon|Florida}} FC Naples (USL1)

|goals1 =

• Salgado {{goal|88}}

|goals2 =

• Henderlong {{goal|79}}
• O'Connor {{goal|90+1}}

|stadium = Premier Sports Campus

|location = Lakewood Ranch, Florida

|attendance = 2,248

|referee = Mazdid Coric

}}

{{football box collapsible

|date = March 18

|time = 7:00 p.m. EDT

|team1 = Miami FC (USLC) {{flagicon|Florida}}

|score = 4–1

|report = https://www.espn.com/soccer/match/_/gameId/732261/naples-united-fc-miami-fc

|team2 = {{flagicon|Florida}} Naples United FC (NPSL)

|goals1 =

• Bonfiglio {{goal|41||45+1}}
• Gavilanes {{goal|66}}
• Zárate {{goal|69}}

|goals2 =

• Moreno {{goal|83}}

|stadium = FIU Soccer Stadium

|location = Miami, Florida

|attendance =

|referee = Edson Carvajal

}}

{{football box collapsible

|date = March 18

|time = 7:00 p.m. EDT

|team1 = New Jersey Alliance FC (UPSL) {{flagicon|New Jersey}}

|score = 2–2

|aet = y

|penaltyscore = 2–4

|report = https://www.espn.com/soccer/match/_/gameId/732259/chattanooga-red-wolves-new-jersey-alliance-fc

|team2 = {{flagicon|Tennessee}} Chattanooga Red Wolves SC (USL1)

|goals1 =

• Preciado {{goal|45+1}}
• Quist {{goal|90+2}}

|goals2 =

• Bentley {{goal|65|pen.|84}}

|penalties1 =

• Quist {{pengoal}}
• Villegas {{penmiss}}
• Rodzen {{penmiss}}
• Hurtado {{pengoal}}

|penalties2 =

• {{pengoal}} Bentley
• {{pengoal}} Ualefi
• {{pengoal}} Smith
• {{pengoal}} Green

|stadium = Lubetkin Field

|location = Newark, New Jersey

|attendance = 200

|referee = Danielle Cornetta

}}

{{football box collapsible

|date = March 18

|time = 7:30 p.m. EDT

|team1 = FC Motown (NPSL) {{flagicon|New Jersey}}

|score = 0–1

|report = https://www.espn.com/soccer/match/_/gameId/732263/westchester-sc-fc-motown

|team2 = {{flagicon|New York}} Westchester SC (USL1)

|goals1 =

|goals2 =

• Obregón {{goal|81|pen.}}

|stadium = MSU Soccer Park at Pittser Field

|location = Montclair, New Jersey

|attendance = 534

|referee = Caleb Firl

}}

{{football box collapsible

|date = March 18

|time = 7:30 p.m. EDT

|team1 = Asheville City SC (USL2) {{flagicon|North Carolina}}

|score = 0–0

|aet = y

|penaltyscore = 3–4

|report = https://www.espn.com/soccer/match/_/gameId/732262/greenville-triumph-asheville-city-sc

|team2 = {{flagicon|South Carolina}} Greenville Triumph SC (USL1)

|goals1 =

|goals2 =

|penalties1 =

• Obara {{pengoal}}
• Morgan {{pengoal}}
• Strömsten {{penmiss}}
• Lyons {{pengoal}}
• Fenwick {{penmiss}}

|penalties2 =

• {{pengoal}} Evans
• {{pengoal}} Polak
• {{pengoal}} Fricke
• {{penmiss}} Mensah
• {{pengoal}} Velásquez

|stadium = Greenwood Field

|location = Asheville, North Carolina

|attendance = 1,000

|referee = John Rush

}}

{{football box collapsible

|date = March 18

|time = 7:30 p.m. EDT

|team1 = West Chester United SC (USLPA) {{flagicon|Pennsylvania}}

|score = 2–3

|report = https://www.espn.com/soccer/match/_/gameId/732264/loudoun-united-fc-west-chester

|team2 = {{flagicon|Virginia}} Loudoun United FC (USLC)

|goals1 =

• Elkahloun {{goal|18|pen.}}
• Roby {{goal|68}}

|goals2 =

• Bidois {{goal|37||61}}
• Sorto {{goal|49|pen.}}

|stadium = YSC Sports

|location = Wayne, Pennsylvania

|attendance = 120

|referee = Joshua Encarnación

}}

{{football box collapsible

|date = March 18

|time = 7:00 p.m. CDT

|team1 = Texoma FC (USL1) {{flagicon|Texas}}

|score = 1–2

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/732265/foro-sc-texoma-fc

|team2 = {{flagicon|Texas}} Foro SC (UPSL)

|goals1 =

• McManus {{goal|59}}

|goals2 =

• Padilla {{goal|15|pen.|99}}

|stadium = Historic Bearcat Stadium

|location = Sherman, Texas

|attendance = 350

|referee = Reyes Vargas

}}

{{football box collapsible

|date = March 18

|time = 7:30 p.m. CDT

|team1 = Tulsa Athletic (TLFC) {{flagicon|Oklahoma}}

|score = 0–1

|report = https://www.espn.com/soccer/match/_/gameId/732266/fc-tulsa-tulsa-athletic

|team2 = {{flagicon|Oklahoma}} FC Tulsa (USLC)

|goals1 =

|goals2 =

• Calheira {{goal|84|pen.}}

|stadium = Athletic Community Field at Hicks Park

|location = Tulsa, Oklahoma

|attendance =

|referee = Rodrigo Albuquerque

}}

{{football box collapsible

|date = March 18

|time = 7:30 p.m. PDT

|team1 = AV Alta FC (USL1) {{flagicon|California}}

|score = 3–1

|report = https://www.espn.com/soccer/match/_/gameId/732267/ventura-county-fusion-av-alta-fc

|team2 = {{flagicon|California}} Ventura County Fusion (USL2)

|goals1 =

• Desdunes {{goal|28||45+3|pen.}}
• Cruz {{goal|52}}

|goals2 =

• Fofanah {{goal|42}}

|stadium = Lancaster Municipal Stadium

|location = Lancaster, California

|attendance = 2,453

|referee = Felix Granados

}}

{{football box collapsible

|date = March 18

|time = 7:30 p.m. PDT

|team1 = Ventura County FC (MLSNP) {{flagicon|California}}

|score = 7–0

|report = https://www.espn.com/soccer/match/_/gameId/732279/laguna-united-fc-ventura-county-fc

|team2 = {{flagicon|California}} Laguna United FC (UPSL)

|goals1 =

• Essengue {{goal|18}}
• Bibout {{goal|41||52||68}}
• Bucknor {{goal|61}}
• Müller {{goal|63}}
• Nicholson {{goal|89|o.g.}}

|goals2 =

|stadium = Dignity Health Sports Park

|location = Carson, California

|attendance = 200

|referee = Rafael Bonilla

}}

{{football box collapsible

|date = March 19

|time = 6:00 p.m. EDT

|team1 = Columbus Crew 2 (MLSNP) {{flagicon|Ohio}}

|score = 4–1

|report = https://www.espn.com/soccer/match/_/gameId/732268/ny-renegades-fc-columbus-crew-2

|team2 = {{flagicon|New York}} NY Renegades (UPSL)

|goals1 =

• Ferdinand {{goal|44}}
• Adu-Gyamfi {{goal|45+3||69}}
• Brown {{goal|62}}

|goals2 =

• Silveira {{goal|79}}

|stadium = Lower.com Field

|location = Columbus, Ohio

|attendance = 620

|referee = Atahan Yaya

}}

{{football box collapsible

|date = March 19

|time = 6:30 p.m. EDT

|team1 = Chattanooga FC (MLSNP) {{flagicon|Tennessee}}

|score = 1–0

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/732269/corpus-christi-fc-chattanooga-fc

|team2 = {{flagicon|Texas}} Corpus Christi FC (USL2)

|goals1 =

• Naglestad {{goal|112}}

|goals2 =

|stadium = Finley Stadium

|location = Chattanooga, Tennessee

|attendance = 2,075

|referee = Wes Caouette

}}

{{football box collapsible

|date = March 19

|time = 7:00 p.m. EDT

|team1 = Appalachian FC (NPSL) {{flagicon|North Carolina}}

|score = 0–3

|report = https://www.espn.com/soccer/match/_/gameId/732272/one-knoxville-appalachian-fc

|team2 = {{flagicon|Tennessee}} One Knoxville SC (USL1)

|goals1 =

|goals2 =

• Santos {{goal|16}}
• Diene {{goal|63}}
• McLeod {{goal|74}}

|stadium = ASU Soccer Stadium

|location = Boone, North Carolina

|attendance = 2,452

|referee = Josiah Parke

}}

{{football box collapsible

|date = March 19

|time = 7:00 p.m. EDT

|team1 = Richmond Kickers (USL1) {{flagicon|Virginia}}

|score = 1–3

|report = https://www.espn.com/soccer/match/_/gameId/732271/virginia-dream-fc-richmond-kickers

|team2 = {{flagicon|Virginia}} Virginia Dream FC (VSSL)

|goals1 =

• Vaughan {{goal|36}}

|goals2 =

• Lukulia {{goal|8|pen.}}
• Suchecki {{goal|53}}
• Akinkoye {{goal|61}}

|stadium = City Stadium

|location = Richmond, Virginia

|attendance = 1,765

|referee = John Griggs

}}

{{football box collapsible

|date = March 19

|time = 7:00 p.m. EDT

|team1 = Inter Miami CF II (MLSNP) {{flagicon|Florida}}

|score = 4–2

|report = https://www.espn.com/soccer/match/_/gameId/732270/miami-united-fc-inter-miami-cf-ii

|team2 = {{flagicon|Florida}} Miami United FC (UPSL)

|goals1 =

• Zeltzer-Zubida {{goal|49}}
• Saja {{goal|65}}
• Cohen {{goal|70||81}}

|goals2 =

• Frean {{goal|36}}
• Luzuriaga {{goal|83}}

|stadium = Chase Stadium

|location = Fort Lauderdale, Florida

|attendance = 1,500

|referee = Alejo Calume

}}

{{football box collapsible

|date = March 19

|time = 6:30 p.m. CDT

|team1 = Sporting Kansas City II (MLSNP) {{flagicon|Missouri}}

|score = 1–2

|report = https://www.espn.com/soccer/match/_/gameId/732274/des-moines-menace-sporting-kansas-city-ii

|team2 = {{flagicon|Iowa}} Des Moines Menace (USL2)

|goals1 =

• Uderitz {{goal|65}}

|goals2 =

• Kljestan {{goal|57|pen.|74|pen.}}

|stadium = Swope Soccer Village

|location = Kansas City, Missouri

|attendance =

|referee = Velemir Stefanovic

}}

{{football box collapsible

|date = March 19

|time = 7:30 p.m. EDT

|team1 = Lexington SC (USLC) {{flagicon|Kentucky}}

|score = 5–0

|report = https://www.espn.com/soccer/match/_/gameId/732273/southern-indiana-fc-lexington

|team2={{flagicon|Indiana}} Southern Indiana FC (UPSL)

|goals1 =

• Burke {{goal|13}}
• Goldthorp {{goal|42}}
• Midence {{goal|46}}
• Stauffer {{goal|53}}
• Rodrigues {{goal|80}}

|goals2 =

|stadium = Lexington SC Stadium

|location = Lexington, Kentucky

|attendance = 1,835

|referee = Rogelio Alvarez

}}

{{football box collapsible

|date = March 19

|time = 7:00 p.m. CDT

|team1 = Little Rock Rangers (USL2) {{flagicon|Arkansas}}

|score = 1–0

|report = https://www.espn.com/soccer/match/_/gameId/732275/birmingham-legion-fc-little-rock-rangers

|team2 = {{flagicon|Alabama}} Birmingham Legion (USLC)

|goals1 =

• McConnell {{goal|48}}

|goals2 =

|stadium = Episcopal Collegiate School

|location = Little Rock, Arkansas

|attendance = 1,328

|referee = Deshun Beard

}}

{{football box collapsible

|date = March 19

|time = 7:00 p.m. MDT

|team1 = El Paso Locomotive FC (USLC) {{flagicon|Texas}}

|score = 5–1

|report = https://www.espn.com/soccer/match/_/gameId/732276/fc-boulder-harpos-el-paso-locomotive-fc

|team2 = {{flagicon|Colorado}} Harpos FC (CPL)

|goals1 =

• Avila {{goal|12}}
• Romero {{goal|37||45+2}}
• Fidelak {{goal|54|o.g.}}
• Torres {{goal|59}}

|goals2 =

• Debuire {{goal|65}}

|stadium = Southwest University Park

|location = El Paso, Texas

|attendance = 1,565

|referee = Melvin Rivas

}}

{{football box collapsible

|date = March 19

|time = 7:30 p.m. MDT

|team1 = Real Monarchs (MLSNP) {{flagicon|Utah}}

|score = 1–3

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/732277/el-farolito-real-monarchs-slc

|team2 = {{flagicon|California}} El Farolito (NPSL)

|goals1 =

• Riquelme {{goal|58}}

|goals2 =

• Benson {{goal|18}}
• Soto {{goal|114|pen.}}
• Villalta {{goal|120+5}}

|stadium = Zions Bank Stadium

|location = Herriman, Utah

|attendance = 496

|referee = Nathan Boone

}}

{{football box collapsible

|date = March 19

|time = 7:00 p.m. PDT

|team1 = Monterey Bay FC (USLC) {{flagicon|California}}

|score = 4–0

|report = https://www.espn.com/soccer/match/_/gameId/732278/inter-san-francisco-monterey-bay

|team2 = {{flagicon|California}} International San Francisco (SFSFL)

|goals1 =

• Fehr {{goal|2}}
• Gnaulati {{goal|26||45+2}}
• Paul {{goal|84}}

|goals2 =

|stadium = Cardinale Stadium

|location = Seaside, California

|attendance = 968

|referee = Robert Vincze

}}

{{football box collapsible

|date = March 19

|time = 7:30 p.m. PDT

|team1 = Ballard FC (USL2) {{flagicon|Washington}}

|score = 0–1

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/732281/spokane-velocity-fc-ballard-fc

|team2 = {{flagicon|Washington}} Spokane Velocity FC (USL1)

|goals1 =

|goals2 =

• Peláez {{goal|120+1}}

|stadium = Interbay Stadium

|location = Seattle, Washington

|attendance = 1,508{{cite press release |date=March 20, 2025 |title=Ballard FC Suffers Heartbreaking Open Cup Defeat to Spokane Velocity in Extra Time |url=https://www.goballardfc.com/news/2025/03/20/ballard-fc-suffers-heartbreaking-open-cup-defeat-to-spokane-velocity-in-extra-time/ |publisher=Ballard FC |accessdate=March 31, 2025}}

|referee = Kelsey Harms

}}

{{football box collapsible

|date = March 19

|time = 7:30 p.m. PDT

|team1 = Los Angeles FC 2 (MLSNP) {{flagicon|California}}

|score = 7–1

|report = https://www.espn.com/soccer/match/_/gameId/732280/fc-arizona-lafc-2

|team2 = {{flagicon|Arizona}} FC Arizona (NPSL)

|goals1 =

• Evans {{goal|2||65}}
• Terry {{goal|27||48}}
• Mihalić {{goal|42}}
• Nava {{goal|82}}
• Walters {{goal|90+3}}

|goals2 =

• Waters {{goal|59}}

|stadium = Titan Stadium

|location = Fullerton, California

|attendance =

|referee = Steven Cardozo

}}

{{football box collapsible

|date = March 20

|time = 6:30 p.m. EDT

|team1 = Portland Hearts of Pine (USL1) {{flagicon|Maine}}

|score = 4–0

|report = https://www.espn.com/soccer/match/_/gameId/732282/cd-faialense-portland-hearts-of-pine

|team2 = {{flagicon|Massachusetts}} CD Faialense (BSSL)

|goals1 =

• Varela {{goal|17}}
• Liadi {{goal|29||62}}
• Messer {{goal|68}}

|goals2 =

|stadium = Franklin Athletic Complex

|location = Lewiston, Maine

|attendance = 3,500

|referee = Andrew Porada

}}

{{football box collapsible

|date = March 20

|time = 7:00 p.m. EDT

|team1 = Long Island Rough Riders (USL2) {{flagicon|New York}}

|score = 2–3

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/732285/charlotte-independence-long-island-rough-riders

|team2 = {{flagicon|North Carolina}} Charlotte Independence (USL1)

|goals1 =

• Woznicki {{goal|49}}
• Khodri {{goal|105+1}}

|goals2 =

• Jauregui {{goal|79||107}}
• Chaney {{goal|104}}

|stadium = Mitchel Athletic Complex

|location = Uniondale, New York

|attendance = 719

|referee = Ignacio Guzman

}}

{{football box collapsible

|date = March 20

|time = 6:00 p.m. CDT

|team1 = Forward Madison FC (USL1) {{flagicon|Wisconsin}}

|score = 5–1

|report = https://www.espn.com/soccer/match/_/gameId/732284/duluth-fc-forward-madison-fc

|team2 = {{flagicon|Minnesota}} Duluth FC (NPSL)

|goals1 =

• Sousa {{goal|39}}
• Munjoma {{goal|73}}
• Galindrez {{goal|86||90+6}}
• Murphy {{goal|90+3}}

|goals2 =

• Fernandez {{goal|64}}

|stadium = Breese Stevens Field

|location = Madison, Wisconsin

|attendance =

|referee = Peter Bernardy

}}

{{football box collapsible

|date = March 20

|time = 7:00 p.m. EDT

|team1 = South Georgia Tormenta FC (USL1) {{flagicon|Georgia (U.S. state)}}

|score = 3–0

|report = https://www.espn.com/soccer/match/_/gameId/732283/harbor-city-fc-south-georgia-tormenta-fc

|team2 = {{flagicon|Florida}} Harbor City FC (UPSL)

|goals1 =

• Nyandjo {{goal|23}}
• Reid-Stephen {{goal|75||80}}

|goals2 =

|stadium = Tormenta Stadium

|location = Statesboro, Georgia

|attendance = 667

|referee = Amin Hadzic

}}

{{football box collapsible

|date = March 20

|time = 7:30 p.m. EDT

|team1 = Carolina Core FC (MLSNP) {{flagicon|North Carolina}}

|score = 4–2

|report = https://www.espn.com/soccer/match/_/gameId/732286/soda-city-fc-carolina-core-fc

|team2 = {{flagicon|South Carolina}} Soda City FC (UPSL)

|goals1 =

• Jo. Rodríguez {{goal|18|pen.|58}}
• Canete {{goal|69}}
• Evans {{goal|72}}

|goals2 =

• Thompson {{goal|79}}
• Salifu {{goal|83}}

|stadium = Truist Point

|location = High Point, North Carolina

|attendance = 2,511

|referee = Joe Surgan

}}

{{football box collapsible

|date = March 20

|time = 7:30 p.m. EDT

|team1 = FC Cincinnati 2 (MLSNP) {{flagicon|Ohio}}

|score = 0–0

|aet = y

|penaltyscore = 4–5

|report = https://www.espn.com/soccer/match/_/gameId/732287/new-york-pancyprian-freedoms-fc-cincinnati-2

|team2 = {{flagicon|New York}} New York Pancyprian-Freedoms (EPSL)

|goals1 =

|goals2 =

|penalties1 =

• Ikoba {{pengoal}}
• Chávez {{pengoal}}
• Machado {{pengoal}}
• Schaefer {{pengoal}}
• Kristel {{penmiss}}
• Kuisel {{penmiss}}

|penalties2 =

• {{pengoal}} Holland
• {{penmiss}} Argudo
• {{pengoal}} Rosero
• {{pengoal}} Sluys
• {{pengoal}} Bailon
• {{pengoal}} Hoff

|stadium = NKU Soccer Stadium

|location = Highland Heights, Kentucky

|attendance =

|referee = Salvador Flores

}}

{{football box collapsible

|date = March 20

|time = 7:00 p.m. PDT

|team1 = Tacoma Defiance (MLSNP) {{flagicon|Washington}}

|score = 3–1

|report = https://www.espn.com/soccer/match/_/gameId/732289/washington-athletic-club-tacoma-defiance

|team2 = {{flagicon|Washington}} Washington Athletic Club (SRATS)

|goals1 =

• Carli {{goal|37}}
• Tsukanome {{goal|47||71}}

|goals2 =

• O'Brien {{goal|9}}

|stadium = Starfire Sports Complex

|location = Tukwila, Washington

|attendance = 205

|referee = Alex Wolbert

}}

{{football box collapsible

|date = March 21

|time = 7:00 p.m. MDT

|team1 = Flatirons FC (USL2) {{flagicon|Colorado}}

|score = 1–2

|report = https://www.espn.com/soccer/match/_/gameId/732288/union-omaha-flatirons-fc

|team2 = {{flagicon|Nebraska}} Union Omaha (USL1)

|goals1 =

• Andrews {{goal|84}}

|goals2 =

• Gómez {{goal|28}}
• Gallardo {{goal|73|pen.}}

|stadium = Stermole Soccer Stadium

|location = Golden, Colorado

|attendance = 200

|referee = Lukasz Jozefecki

}}

{{football box collapsible

|date = April 1

|time = 6:00 p.m. EDT

|team1 = Columbus Crew 2 (MLSNP) {{flagicon|Ohio}}

|score = 3–0

|report = https://www.espn.com/soccer/match/_/gameId/734946/lexington-columbus-crew-2

|team2 = {{flagicon|Kentucky}} Lexington SC (USLC)

|goals1 =

• Presthus {{goal|7}}
• Ortiz {{goal|65}}
• Adu-Gyamfi {{goal|90+4}}

|goals2 =

|stadium = Lower.com Field

|location = Columbus, Ohio

|attendance = 378

|referee = Salvador Flores

}}

{{football box collapsible

|date = April 1

|time = 7:00 p.m. EDT

|team1 = South Georgia Tormenta FC (USL1) {{flagicon|Georgia (U.S. state)}}

|score = 3–2

|report = https://www.espn.com/soccer/match/_/gameId/734947

|team2 ={{flagicon|Texas}} Foro SC (UPSL)

|goals1 =

• Walker {{goal|38}}
• Bazini {{goal|85}}
• Vivas {{goal|90+4}}

|goals2 =

• Rayo {{goal|67}}
• Padilla {{goal|90+2|pen.}}

|stadium = Tormenta Stadium

|location = Statesboro, Georgia

|attendance = 467

|referee = Josiah Parke

}}

{{football box collapsible

|date = April 1

|time = 7:30 p.m. EDT

|team1 = Inter Miami CF II (MLSNP) {{flagicon|Florida}}

|score = 0–1

|report = https://www.espn.com/soccer/match/_/gameId/734949/miami-fc-inter-miami-cf-ii

|team2 = {{flagicon|Florida}} Miami FC (USLC)

|goals1 =

|goals2 =

• Bonfiglio {{goal|84}}

|stadium = Chase Stadium

|location = Fort Lauderdale, Florida

|attendance = 1,500

|referee = Johnathan Bilinski

}}

{{football box collapsible

|date = April 1

|time = 7:30 p.m. EDT

|team1 = Charlotte Independence (USL1) {{flagicon|North Carolina}}

|score = 2–1

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/734948/carolina-core-fc-charlotte-independence

|team2 = {{flagicon|North Carolina}} Carolina Core FC (MLSNP)

|goals1 =

• Álvarez {{goal|2}}
• Marou {{goal|93}}

|goals2 =

• Jo. Rodríguez {{goal|10}}

|stadium = Sportsplex at Matthews

|location = Matthews, North Carolina

|attendance =

|referee = John Rush

}}

{{football box collapsible

|date = April 1

|time = 7:00 p.m. PDT

|team1 = Monterey Bay FC (USLC) {{flagicon|California}}

|score = 1–2

|report = https://www.espn.com/soccer/match/_/gameId/734950/el-farolito-monterey-bay

|team2 = {{flagicon|California}} El Farolito SC (NPSL)

|goals1 =

• Gnaulati {{goal|28|pen.}}

|goals2 =

• Zuluaga {{goal|57}}
• Kreye {{goal|83}}

|stadium = Cardinale Stadium

|location = Seaside, California

|attendance = 688

|referee = Esad Omanovic

}}

{{football box collapsible

|date = April 1

|time = 7:30 p.m. PDT

|team1 = AV Alta FC (USL1) {{flagicon|California}}

|score = 2–1

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/734951/lafc-2-av-alta-fc

|team2 = {{flagicon|California}} Los Angeles FC 2 (MLSNP)

|goals1 =

• Alaribe {{goal|80}}
• Villalobos {{goal|120}}

|goals2 =

• Guerra {{goal|64}}

|stadium = Lancaster Municipal Stadium

|location = Lancaster, California

|attendance = 2,735

|referee = Servando Berna Rico

}}

{{football box collapsible

|date = April 2

|time = 6:30 p.m. EDT

|team1 = Loudoun United FC (USLC) {{flagicon|Virginia}}

|score = 4–2

|report = https://www.espn.com/soccer/match/_/gameId/734953/virginia-dream-fc-loudoun-united-fc

|team2 = {{flagicon|Virginia}} Virginia Dream FC (VSSL)

|goals1 =

• Bidois {{goal|42}}
• Mines {{goal|59}}
• Ryan {{goal|77||90+2}}

|goals2 =

• Akinkoye {{goal|50}}
• Likulia {{goal|86}}

|stadium = Segra Field

|location = Leesburg, Virginia

|attendance =

|referee = Marie Durr

}}

{{football box collapsible

|date = April 2

|time = 6:30 p.m. EDT

|team1 = Portland Hearts of Pine (USL1) {{flagicon|Maine}}

|score = 1–1

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/734952/hartford-athletic-portland-hearts-of-pine

|team2 = {{flagicon|Connecticut}} Hartford Athletic (USLC)

|goals1 =

• Keegan {{goal|117}}

|goals2 =

• Scarlett {{goal|107}}

|penalties1=

• Langlois {{pengoal}}
• Keegan {{pengoal}}
• Messer {{pengoal}}
• Southern {{pengoal}}

|penaltyscore=4–2

|penalties2=

• {{pengoal}} Farrell
• {{pengoal}} Dieng
• {{penmiss}} Anderson
• {{penmiss}} Ngalina

|stadium = Franklin Athletic Complex

|location = Lewiston, Maine

|attendance =3,002

|referee =Kyle Averill

}}

{{football box collapsible

|date = April 2

|time = 7:00 p.m. EDT

|team1 = Westchester SC (USL1) {{flagicon|New York}}

|score = 3–2

|report = https://www.espn.com/soccer/match/_/gameId/734954/new-york-pancyprian-freedoms-westchester-sc

|team2 = {{flagicon|New York}} New York Pancyprian-Freedoms (EPSL)

|goals1 =

• Obregón {{goal|3}}
• Sluys {{goal|22|o.g.}}
• Bouman {{goal|34}}

|goals2 =

• Martín {{goal|54}}
• Thristino {{goal|90+1}}

|stadium = The Stadium at Memorial Field

|location = Mount Vernon, New York

|attendance = 2,026

|referee =

}}

{{football box collapsible

|date = April 2

|time = 7:00 p.m. EDT

|team1 = Chattanooga FC (MLSNP) {{flagicon|Tennessee}}

|score = 1–1

|aet = y

|report = https://www.espn.com/soccer/match/_/gameId/734955/chattanooga-red-wolves-chattanooga-fc

|team2 = {{flagicon|Tennessee}} Chattanooga Red Wolves SC (USL1)

|goals1 =

• Mangarov {{goal|19}}

|goals2 =

• Hernandez {{goal|7}}

|penalties1=

• Mangarov {{penmiss}}
• Screen {{pengoal}}
• Naglestad {{pengoal}}
• Sar-Sar {{pengoal}}
• Dudley {{pengoal}}

|penaltyscore=4–5

|penalties2=

• {{pengoal}} Bentley
• {{pengoal}} Vazquez
• {{pengoal}} Filipe
• {{pengoal}} Hernández
• {{pengoal}} Green

|stadium = Finley Stadium

|location = Chattanooga, Tennessee

|attendance = 12,131

|referee = Richonne Clark

}}

{{football box collapsible

|date = April 2

|time = 7:00 p.m. EDT

|team1 = Greenville Triumph SC (USL1) {{flagicon|South Carolina}}

|score = 1–3

|report = https://www.espn.com/soccer/match/_/gameId/734956

|team2 = {{flagicon|Tennessee}} One Knoxville SC (USL1)

|goals1 =

• Mensah {{goal|55}}

|goals2 =

• Zarokostas {{goal|14}}
• Doyle {{goal|23}}
• Diene {{goal|90+5}}

|stadium = Paladin Stadium

|location = Greenville, South Carolina

|attendance = 854

|referee = Izlen Peksenar

}}

{{football box collapsible

|date = April 2

|time = 7:30 p.m. EDT

|team1 = FC Naples (USL1) {{flagicon|Florida}}

|score = 3–0

|report = https://www.espn.com/soccer/match/_/gameId/734958/little-rock-rangers-fc-naples

|team2 = {{flagicon|Arkansas}} Little Rock Rangers (USL2)

|goals1 =

• Onen {{goal|1}}
• Evans {{goal|14}}
• Ferrín {{goal|77}}

|goals2 =

|stadium = Paradise Coast Sports Complex Stadium

|location = Naples, Florida

|attendance = 2,174

|referee = Edson Caravajal

}}

{{football box collapsible

|date = April 2

|time = 6:30 p.m. CDT

|team1 = Forward Madison FC (USL1) {{flagicon|Wisconsin}}

|score = 1–3

|report = https://www.espn.com/soccer/match/_/gameId/734957/fc-tulsa-forward-madison-fc

|team2 = {{flagicon|Oklahoma}} FC Tulsa (USLC)

|goals1 =

• Bartman {{goal|68}}

|goals2 =

• Damm {{goal|21}}
• ElMedkhar {{goal|31}}
• Calheira {{goal|51|pen.}}

|stadium = Breese Stevens Field

|location = Madison, Wisconsin

|attendance = 832

|referee = Felix Granados

}}

{{football box collapsible

|date = April 2

|time = 7:30 p.m. CDT

|team1 = Des Moines Menace (USL2) {{flagicon|Iowa}}

|score = 1–2

|report = https://www.espn.com/soccer/match/_/gameId/734959/union-omaha-des-moines-menace

|team2 = {{flagicon|Nebraska}} Union Omaha (USL1)

|goals1 =

• Feilhaber {{goal|55|pen.}}

|goals2 =

• Acoff {{goal|18}}
• Gómez {{goal|33}}

|stadium = Valley Stadium

|location = West Des Moines, Iowa

|attendance = 1,000

|referee = Christian Campo Hernandez

}}

{{football box collapsible

|date = April 2

|time = 7:00 p.m. PDT

|team1 = Tacoma Defiance (MLSNP) {{flagicon|Washington}}

|score = 2–1

|report = https://www.espn.com/soccer/match/_/gameId/734960

|team2 = {{flagicon|Washington}} Spokane Velocity FC (USL1)

|goals1 =

• Kang {{goal|3}}
• Lage {{goal|99|o.g.}}

|goals2 =

• Gil {{goal|78}}

|stadium = Starfire Sports Complex

|location = Tukwila, Washington

|attendance = 2,300

|referee = Christopher Calderon

}}

{{football box collapsible

|date = April 2

|time = 7:30 p.m. PDT

|team1 = Ventura County FC (MLSNP) {{flagicon|California}}

|score = 0–3

|report = https://www.espn.com/soccer/match/_/gameId/734961

|team2 = {{flagicon|Texas}} El Paso Locomotive FC (USLC)

|goals1 =

|goals2 =

• Carter {{goal|17}}
• Vásquez {{goal|29}}
• Cabrera Jr. {{goal|81|}}

|stadium = Dignity Health Sports Park Track & Field Stadium

|location = Thousand Oaks, California

|attendance = 50

|referee = Steven Cardozo

}}

The complete draw for the third round, including match dates and times, was announced on April 3, 2025.{{Cite web |date=2025-04-03 |title=Schedule & Results {{!}} 2025 U.S. Open Cup |url=https://www.ussoccer.com/us-open-cup/schedule |access-date=2025-04-03 |website=www.ussoccer.com |language=en}}{{football box collapsible

|date = April 15

|time =6:00 p.m. EDT

|team1 =Columbus Crew 2 (MLSNP) {{flagicon|Ohio}}

|score = 0–1

|report= https://www.espn.com/soccer/match/_/gameId/735163/pittsburgh-riverhounds-columbus-crew-2

|team2 ={{flagicon|Pennsylvania}} Pittsburgh Riverhounds SC (USLC)

|goals1 =

|goals2 =

• Griffin {{goal|28}}

|aet =

| stadium =Lower.com Field

| location =Columbus, Ohio

|attendance = 376

|referee = Rogelio Alvarez

|result=

}}

{{football box collapsible

|date = April 15

|time =7:00 p.m. EDT

|team1 =Louisville City FC (USLC) {{flagicon|Kentucky}}

|score = 2–1

|report= https://www.espn.com/soccer/match/_/gameId/735168/loudoun-united-fc-louisville-city-fc

|team2 ={{flagicon|Virginia}} Loudoun United FC (USLC)

|goals1 =

• Perez {{goal|18}}
• Gleadle {{goal|28}}

|goals2 =

• Aboukoura {{goal|90}}

|aet =

| stadium =Lynn Family Stadium

| location =Louisville, Kentucky

|attendance = 2,642

|referee = Elivis Osmanovic

|result=

}}

{{football box collapsible

|date = April 15

|time =7:00 p.m. EDT

|team1 =Charlotte Independence (USL1) {{flagicon|North Carolina}}

|score = 1–3

|report= https://www.espn.com/soccer/match/_/gameId/735161/north-carolina-fc-charlotte-independence

|team2 ={{flagicon|North Carolina}} North Carolina FC (USLC)

|goals1 =

• Bakero {{goal|48}}

|goals2 =

• Anderson {{goal|71}}
• da Costa {{goal|74||77}}

|aet =

| stadium =Sportsplex at Matthews

| location =Matthews, North Carolina

|attendance =

|referee = Danielle Chesney

|result=

}}

{{football box collapsible

|date = April 15

|time =7:00 p.m. EDT

|team1 =Portland Hearts of Pine (USL1) {{flagicon|Maine}}

|score = 1–2

|report= https://www.espn.com/soccer/match/_/gameId/735171/rhode-island-fc-portland-hearts-of-pine

|team2 ={{flagicon|Rhode Island}} Rhode Island FC (USLC)

|goals1 =

• Washington {{goal|65}}

|goals2 =

• Holstad {{goal|34}}
• Nodarse {{goal|37}}

|aet =

| stadium =Franklin Athletic Complex

| location =Lewiston, Maine

|attendance = 2,933

|referee = Thomas Snyder

|result=

}}

{{football box collapsible

|date = April 15

|time =7:30 p.m. EDT

|team1 =Charleston Battery (USLC) {{flagicon|South Carolina}}

|score = 4–0

|report= https://www.espn.com/soccer/match/_/gameId/735174

|team2 ={{flagicon|Georgia (U.S. state)}} South Georgia Tormenta FC (USL1)

|goals1 =

• Allan {{goal|32}}
• Myers {{goal|45+1||52||70}}

|goals2 =

|aet =

| stadium =Ralph Lundy Field

| location =Mount Pleasant, South Carolina

|attendance = 1,419

|referee = John Rush

|result=

}}

{{football box collapsible

|date = April 15

|time =7:00 p.m. CDT

|team1 =Union Omaha (USL1) {{flagicon|Nebraska}}

|score = 1–0

|report= https://www.espn.com/soccer/match/_/gameId/735160

|team2 ={{flagicon|Texas}} San Antonio FC (USLC)

|goals1 =

• Acoff {{goal|36}}

|goals2 =

|aet =

| stadium =Caniglia Field

| location =Omaha, Nebraska

|attendance =

|referee = Drew Klemp

|result=

}}

{{football box collapsible

|date = April 15

|time =7:00 p.m. PDT

|team1 =AV Alta FC (USL1) {{flagicon|California}}

|score = 2–2

|report= https://www.espn.com/soccer/match/_/gameId/735159/orange-county-sc-av-alta-fc

|team2 ={{flagicon|California}} Orange County SC (USLC)

|goals1 =

• Mastrantonio {{goal|67}}
• Alaribe {{goal|75}}

|goals2 =

• Sylla {{goal|15}}
• War {{goal|83}}

|aet = y

|penalties1=

• Cerritos {{pengoal}}
• Blancos {{pengoal}}
• Cruz {{pengoal}}
• Pajaro {{pengoal}}

|penaltyscore=4–2

|penalties2=

• {{pengoal}} Dunbar
• {{pengoal}} MacKinnon
• {{penmiss}} Scott
• {{penmiss}} Benalcazar

| stadium =Lancaster Municipal Stadium

| location =Lancaster, California

|attendance =

|referee =

|result=

}}

{{football box collapsible

|date = April 15

|time =7:30 p.m. PDT

|team1 =Tacoma Defiance (MLSNP) {{flagicon|Washington}}

|score = 2–1

|report= https://www.espn.com/soccer/match/_/gameId/735173/oakland-roots-tacoma-defiance

|team2 ={{flagicon|California}} Oakland Roots SC (USLC)

|goals1 =

• Kingston {{goal|28}}
• De Rosario {{goal|108}}

|goals2 =

• Hackshaw {{goal|75|pen.}}

|aet = y

| stadium =Starfire Sports Complex

| location =Tukwila, Washington

|attendance =

|referee =

|result=

}}

{{football box collapsible

|date = April 16

|time =7:00 p.m. EDT

|team1 =Detroit City FC (USLC) {{flagicon|Michigan}}

|score = 3–1

|report= https://www.espn.com/soccer/match/_/gameId/735164/westchester-sc-detroit-city-fc

|team2 ={{flagicon|New York}} Westchester SC (USL1)

|goals1 =

• Doner {{goal|71}}
• Cedeno {{goal|85}}
• Smith {{goal|90+4}}

|goals2 =

• Obregón {{goal|76}}

|aet =

| stadium =Keyworth Stadium

| location =Hamtramck, Michigan

|attendance =

|referee = Edson Carvajal

|result=

}}

{{football box collapsible

|date = April 16

|time =7:00 p.m. EDT

|team1 =FC Naples (USL1) {{flagicon|Florida}}

|score = 1–1

|report= https://www.espn.com/soccer/match/_/gameId/735169/tampa-bay-rowdies-fc-naples

|team2 = Tampa Bay Rowdies (USLC) {{flagicon|Florida}}

|goals1 =

• Prpa {{goal|27}}

|goals2 =

• Niyongabire {{goal|26}}

|aet = y

|penalties1=

• Henderlong {{pengoal}}
• Onen {{pengoal}}
• Dengler {{pengoal}}
• Heckenberg {{penmiss}}
• Cerro {{pengoal}}
• Pasnik {{pengoal}}
• Evans {{pengoal}}
• Torrellas {{pengoal}}
• Fernandes {{pengoal}}
• Cisneros {{penmiss}}

|penaltyscore=8–9

|penalties2=

• Bassett {{pengoal}}
• Fernandes {{penmiss}}
• MacPherson {{pengoal}}
• Crisotomo {{pengoal}}
• Pacius {{pengoal}}
• Mustali {{pengoal}}
• Castellanos {{pengoal}}
• Skinner {{pengoal}}
• Vancaeyezeele {{pengoal}}
• Lasso {{pengoal}}

| stadium = Paradise Coast Sports Complex Stadium

| location = Naples, Florida

|attendance = 3,418

|referee = Muhammad Hassan

|result=

}}

{{football box collapsible

|date = April 16

|time =7:30 p.m. EDT

|team1 =Indy Eleven (USLC) {{flagicon|Indiana}}

|score = 1–0

|report= https://www.espn.com/soccer/match/_/gameId/735166/miami-fc-indy-eleven

|team2 ={{flagicon|Florida}} Miami FC (USLC)

|goals1 =

• White {{goal|120+3}}

|goals2 =

|aet = y

| stadium =Michael A. Carroll Stadium

| location =Indianapolis, Indiana

|attendance = 4,366

|referee = Calin Radosav

|result=

}}

{{football box collapsible

|date = April 16

|time =7:00 p.m. CDT

|team1 =FC Tulsa (USLC) {{flagicon|Oklahoma}}

|score = 1–1

|report= https://www.espn.com/soccer/match/_/gameId/735165/phoenix-rising-fc-fc-tulsa

|team2 ={{flagicon|Arizona}} Phoenix Rising FC (USLC)

|goals1 =

• Lukic {{goal|86}}

|goals2 =

• Margaritha {{goal|51}}

|aet = y

|penalties1=

• ElMedkhar {{penmiss}}
• Bettache {{penmiss}}
• Rogers {{pengoal}}
• Colli {{pengoal}}

|penaltyscore=2–4

|penalties2=

• {{pengoal}} Cuello
• {{pengoal}} Scearce
• {{pengoal}} Walls
• {{pengoal}} Sainté

| stadium =Hurricane Soccer & Track Stadium

| location =Tulsa, Oklahoma

|attendance =

|referee = Rodrigo Albuquerque

|result=

}}

{{football box collapsible

|date = April 16

|time =7:00 p.m. MDT

|team1 =Colorado Springs Switchbacks FC (USLC) {{flagicon|Colorado}}

|score = 3–2

|report= https://www.espn.com/soccer/match/_/gameId/735162/one-knoxville-colorado-springs-switchbacks-fc

|team2 ={{flagicon|Tennessee}} One Knoxville SC (USL1)

|goals1 =

• Echevarria {{goal|49}}
• Micaletto {{goal|81}}
• Fontana {{goal|103}}

|goals2 =

• Diene {{goal|2}}
• Zarokostas {{goal|83}}

|aet = y

| stadium =Weidner Field

| location =Colorado Springs, Colorado

|attendance =

|referee = Jay Schlesinger

|result=

}}

{{football box collapsible

|date = April 16

|time =7:30 p.m. MDT

|team1 =New Mexico United (USLC) {{flagicon|New Mexico}}

|score = 2–2

|report= https://www.espn.com/soccer/match/_/gameId/735170/el-paso-locomotive-fc-new-mexico-united

|team2 ={{flagicon|Texas}} El Paso Locomotive (USLC)

|goals1 =

• Vargas {{goal|39}}
• Lindsey {{goal|72}}

|goals2 =

• Carter {{goal|21}}
• Cabrera Jr. {{goal|90+7}}

|aet = y

|penalties1=

• Zelalem {{penmiss}}
• Maples {{pengoal}}
• Ryden {{penmiss}}

|penaltyscore=1–4

|penalties2=

• {{pengoal}} Coronado
• {{pengoal}} Ruiz
• {{pengoal}} Torres
• {{pengoal}} Cabrera Jr.

| stadium =UNM Soccer Complex

| location =Albuquerque, New Mexico

|attendance =

|referee = John Griggs

|result=

}}

{{football box collapsible

|date = April 16

|time =7:00 p.m. PDT

|team1 =Sacramento Republic FC (USLC) {{flagicon|California}}

|score = 1–0

|report= https://www.espn.com/soccer/match/_/gameId/735172/el-farolito-sacramento-republic-fc

|team2 ={{flagicon|California}} El Farolito SC (NPSL)

|goals1 =

• Herrera {{goal|29}}

|goals2 =

|aet =

| stadium =Heart Health Park

| location =Sacramento, California

|attendance =

|referee = Robert Vincze

|result=

}}

{{football box collapsible

|date = April 16

|time =7:30 p.m. PDT

|team1 =Las Vegas Lights FC (USLC) {{flagicon|Nevada}}

|score = 2–2

|report= https://www.espn.com/soccer/match/_/gameId/735167/chattanooga-red-wolves-las-vegas-lights-fc

|team2 ={{flagicon|Tennessee}} Chattanooga Red Wolves SC (USL1)

|goals1 =

• Azcona {{goal|43}}
• Pinzón {{goal|62}}

|goals2 =

• Vazquez {{goal|19}}
• Hernández {{goal|48|pen.}}

|aet = y

|penalties1=

• Pinzón {{penmiss}}
• Pearson {{pengoal}}
• O'Driscoll {{pengoal}}
• Noël {{pengoal}}
• Nigro {{penmiss}}

|penaltyscore=3–4

|penalties2=

• {{pengoal}} Bentley
• {{penmiss}} Ualefi
• {{pengoal}} Hernandez
• {{pengoal}} Hernández
• {{pengoal}} Green

| stadium =Cashman Field

| location =Las Vegas, Nevada

|attendance =

|referee = Jaclyn Metz

|result=

}}

The draw for the round of 32 and round of 16 took place live on CBS Sports Golazo Network on April 17, 2025, performed by CBS Sports Golazo Network presenter Aly Trost Martin and U.S. Open Cup commissioner David Applegate. The teams were divided into eight broadly geographical groups named for the eight most-capped United States men's national team players who had also won a U.S. Open Cup: Cobi Jones, Landon Donovan, Clint Dempsey, Jeff Agoos, DaMarcus Beasley, Carlos Bocanegra, Paul Caligiuri and Kasey Keller. The winners of four-team mini-bracket will advance to the quarterfinals. The order in which teams were drawn for the round of 32 determined the priority order for home field advantage in the round of 16.{{cite AV media |people=Aly Trost Martin, David Applegate |date=April 17, 2025 |title= U.S. Open Cup Round of 32 and Round of 16 Draw {{!}} CBS Sports Golazo |url=https://www.youtube.com/watch?v=9VeGwwhcWXs |type=Live stream |publisher=CBS Sports Golazo America |via=YouTube |access-date=April 17, 2025 }}{{cite news |first=Chuck |last=Booth |title=U.S. Open Cup draw results: Sacramento Republic to face former USMNT coach Bruce Arena's San Jose Earthquakes |url=https://www.cbssports.com/soccer/news/u-s-open-cup-draw-results-sacramento-republic-to-face-former-usmnt-coach-bruce-arenas-san-jose-earthquakes/live/ |website=CBS Sports |publisher=CBS Interactive |date=April 17, 2025 |accessdate=April 17, 2025 }}

• Bold denotes teams that are automatically seeded as hosts in the Round of 32.{{Cite web |date=2025-01-28 |title=2025 US Open Cup format revealed: More MLS teams return to competition |url=https://thecup.us/2025/01/28/2025-us-open-cup-format-revealed-more-mls-teams-return-to-competition/ |access-date=2025-04-17 |website=thecup.us }}

Bold denotes the winners of each match.{{Cite web |date=April 17, 2025 |title=U.S. Open Cup draw results: Sacramento Republic to face former USMNT coach Bruce Arena's San Jose Earthquakes |url=https://www.cbssports.com/soccer/news/u-s-open-cup-draw-results-sacramento-republic-to-face-former-usmnt-coach-bruce-arenas-san-jose-earthquakes/live/ |access-date=April 17, 2025 |website=CBS Sports }}

{{2NTeam-NTeamBracket

| rounds = 2

| brackets = 8

| RD1-teams = 4

| boldwinner = y

| seed-width = 50

| team-width = 205

| score-width = 30

| RD1 = Round of 32
May 6–7

| RD2 = Round of 16
May 20–21

| RD1-seed01 = USLC

| RD1-team01 = {{flagicon|Rhode Island}} Rhode Island FC

| RD1-score01 =

| RD1-seed02 = MLS

| RD1-team02 = {{flagicon|Massachusetts}} New England Revolution

| RD1-score02 =

| RD1-seed03 = MLS

| RD1-team03 = {{flagicon|Illinois}} Chicago Fire FC

| RD1-score03 =

| RD1-seed04 = USLC

| RD1-team04 = {{flagicon|Michigan}} Detroit City FC

| RD1-score04 =

| RD1-seed05 = MLS

| RD1-team05 = {{flagicon|Pennsylvania}} Philadelphia Union

| RD1-score05 =

| RD1-seed06 = USLC

| RD1-team06 = {{flagicon|Indiana}} Indy Eleven

| RD1-score06 =

| RD1-seed07 = USLC

| RD1-team07 = {{flagicon|Pennsylvania}} Pittsburgh Riverhounds SC

| RD1-score07 =

| RD1-seed08 = MLS

| RD1-team08 = {{flagicon|New York}} New York City FC

| RD1-score08 =

| RD1-seed09 = USLC

| RD1-team09 = {{flagicon|North Carolina}} North Carolina FC

| RD1-score09 =

| RD1-seed10 = MLS

| RD1-team10 = {{flagicon|North Carolina}} Charlotte FC

| RD1-score10 =

| RD1-seed11 = MLS

| RD1-team11 = {{flagicon|District of Columbia}} D.C. United

| RD1-score11 =

| RD1-seed12 = USLC

| RD1-team12 = {{flagicon|South Carolina}} Charleston Battery

| RD1-score12 =

| RD1-seed13 = USLC

| RD1-team13 = {{flagicon|Florida}} Tampa Bay Rowdies

| RD1-score13 =

| RD1-seed14 = MLS

| RD1-team14 = {{flagicon|Florida}} Orlando City SC

| RD1-score14 =

| RD1-seed15 = MLS

| RD1-team15 = {{flagicon|Tennessee}} Nashville SC

| RD1-score15 =

| RD1-seed16 = USL1

| RD1-team16 = {{flagicon|Tennessee}} Chattanooga Red Wolves SC

| RD1-score16 =

| RD1-seed17 = USLC

| RD1-team17 = {{flagicon|Kentucky}} Louisville City FC

| RD1-score17 =

| RD1-seed18 = MLS

| RD1-team18 = {{flagicon|Minnesota}} Minnesota United FC

| RD1-score18 =

| RD1-seed19 = MLS

| RD1-team19 = {{flagicon|Missouri}} St. Louis City SC

| RD1-score19 =

| RD1-seed20 = USL1

| RD1-team20 = {{flagicon|Nebraska}} Union Omaha

| RD1-score20 =

| RD1-seed21 = USLC

| RD1-team21 = {{flagicon|Arizona}} Phoenix Rising FC

| RD1-score21 =

| RD1-seed22 = MLS

| RD1-team22 = {{flagicon|Texas}} Houston Dynamo FC

| RD1-score22 =

| RD1-seed23 = MLS

| RD1-team23 = {{flagicon|Texas}} Austin FC

| RD1-score23 =

| RD1-seed24 = USLC

| RD1-team24 = {{flagicon|Texas}} El Paso Locomotive FC

| RD1-score24 =

| RD1-seed25 = USLC

| RD1-team25 = {{flagicon|Colorado}} Colorado Springs Switchbacks FC

| RD1-score25 =

| RD1-seed26 = MLS

| RD1-team26 = {{flagicon|New Jersey}} New York Red Bulls

| RD1-score26 =

| RD1-seed27 = MLS

| RD1-team27 = {{flagicon|Texas}} FC Dallas

| RD1-score27 =

| RD1-seed28 = USL1

| RD1-team28 = {{flagicon|California}} AV Alta FC

| RD1-score28 =

| RD1-seed29 = MLSNP

| RD1-team29 = {{flagicon|Washington}} Tacoma Defiance

| RD1-score29 =

| RD1-seed30 = MLS

| RD1-team30 = {{flagicon|Oregon}} Portland Timbers

| RD1-score30 =

| RD1-seed31 = MLS

| RD1-team31 = {{flagicon|California}} San Jose Earthquakes

| RD1-score31 =

| RD1-seed32 = USLC

| RD1-team32 = {{flagicon|California}} Sacramento Republic FC

| RD1-score32 =

| RD2-seed01 =

| RD2-team01 =

| RD2-score01 =

| RD2-seed02 =

| RD2-team02 =

| RD2-score02 =

| RD2-seed03 =

| RD2-team03 =

| RD2-score03 =

| RD2-seed04 =

| RD2-team04 =

| RD2-score04 =

| RD2-seed05 =

| RD2-team05 =

| RD2-score05 =

| RD2-seed06 =

| RD2-team06 =

| RD2-score06 =

| RD2-seed07 =

| RD2-team07 =

| RD2-score07 =

| RD2-seed08 =

| RD2-team08 =

| RD2-score08 =

| RD2-seed09 =

| RD2-team09 =

| RD2-score09 =

| RD2-seed10 =

| RD2-team10 =

| RD2-score10 =

| RD2-seed11 =

| RD2-team11 =

| RD2-score11 =

| RD2-seed12 =

| RD2-team12 =

| RD2-score12 =

| RD2-seed13 =

| RD2-team13 =

| RD2-score13 =

| RD2-seed14 =

| RD2-team14 =

| RD2-score14 =

| RD2-seed15 =

| RD2-team15 =

| RD2-score15 =

| RD2-seed16 =

| RD2-team16 =

| RD2-score16 =

}}

Host team listed first

Bold = winner

Cobi Jones Group

{{footballbox collapsible

|date=May 7, 2025

|time=7:30 p.m. EDT

|team1=Rhode Island FC {{flagicon|Rhode Island}}

|score=

|report=

|team2={{flagicon|Massachusetts}} New England Revolution

|goals1=

|goals2=

|stadium=Centreville Bank Stadium

|location=Pawtucket, Rhode Island

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 7, 2025

|time=7:00 p.m. CDT

|team1=Chicago Fire FC {{flagicon|Illinois}}

|score=

|report=

|team2={{flagicon|Michigan}} Detroit City FC

|goals1=

|goals2=

|stadium=Soldier Field

|location=Chicago, Illinois

|attendance=

|referee=

}}

Landon Donovan Group

{{footballbox collapsible

|date=May 7, 2025

|time=7:00 p.m. EDT

|team1=Pittsburgh Riverhounds SC {{flagicon|Pennsylvania}}

|score=

|report=

|team2={{flagicon|New York}} New York City FC

|goals1=

|goals2=

|stadium=Highmark Stadium

|location=Pittsburgh, Pennsylvania

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 7, 2025

|time=7:30 p.m. EDT

|team1=Philadelphia Union {{flagicon|Pennsylvania}}

|score=

|report=

|team2={{flagicon|Indiana}} Indy Eleven

|goals1=

|goals2=

|stadium=Subaru Park

|location=Chester, Pennsylvania

|attendance=

|referee=

}}

Clint Dempsey Group

{{footballbox collapsible

|date=May 6, 2025

|time=7:00 p.m. EDT

|team1=North Carolina FC {{flagicon|North Carolina}}

|score=

|report=

|team2={{flagicon|North Carolina}} Charlotte FC

|goals1=

|goals2=

|stadium=WakeMed Soccer Park

|location=Cary, North Carolina

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 6, 2025

|time=7:00 p.m. EDT

|team1=D.C. United {{flagicon|District of Columbia}}

|score=

|report=

|team2={{flagicon|South Carolina}} Charleston Battery

|goals1=

|goals2=

|stadium=Audi Field

|location=Washington, D.C.

|attendance=

|referee=

}}

Jeff Agoos Group

{{footballbox collapsible

|date=May 7, 2025

|time=7:30 p.m. EDT

|team1=Tampa Bay Rowdies {{flagicon|Florida}}

|score=

|report=

|team2={{flagicon|Florida}} Orlando City SC

|goals1=

|goals2=

|stadium=Al Lang Stadium

|location=St. Petersburg, Florida

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 6, 2025

|time=7:00 p.m. CDT

|team1=Nashville SC {{flagicon|Tennessee}}

|score=

|report=

|team2={{flagicon|Tennessee}} Chattanooga Red Wolves SC

|goals1=

|goals2=

|stadium=Geodis Park

|location=Nashville, Tennessee

|attendance=

|referee=

}}

DaMarcus Beasley Group

{{footballbox collapsible

|date=May 7, 2025

|time=7:30 p.m. EDT

|team1=Louisville City FC {{flagicon|Kentucky}}

|score=

|report=

|team2={{flagicon|Minnesota}} Minnesota United FC

|goals1=

|goals2=

|stadium=Lynn Family Stadium

|location=Louisville, Kentucky

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 7, 2025

|time=7:00 p.m. CDT

|team1=St. Louis City SC {{flagicon|Missouri}}

|score=

|report=

|team2={{flagicon|Nebraska}} Union Omaha

|goals1=

|goals2=

|stadium=Energizer Park

|location=St. Louis, Missouri

|attendance=

|referee=

}}

Carlos Bocanegra Group

{{footballbox collapsible

|date=May 7, 2025

|time=7:00 p.m. MST

|team1=Phoenix Rising FC {{flagicon|Arizona}}

|score=

|report=

|team2={{flagicon|Texas}} Houston Dynamo FC

|goals1=

|goals2=

|stadium=Phoenix Rising Soccer Stadium

|location=Phoenix, Arizona

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 7, 2025

|time=7:30 p.m. CDT

|team1=Austin FC {{flagicon|Texas}}

|score=

|report=

|team2={{flagicon|Texas}} El Paso Locomotive FC

|goals1=

|goals2=

|stadium=Q2 Stadium

|location=Austin, Texas

|attendance=

|referee=

}}

Paul Caligiuri Group

{{footballbox collapsible

|date=May 6, 2025

|time=7:30 p.m. MDT

|team1=Colorado Springs Switchbacks FC {{flagicon|Colorado}}

|score=

|report=

|team2={{flagicon|New Jersey}} New York Red Bulls

|goals1=

|goals2=

|stadium=Weidner Field

|location=Colorado Springs, Colorado

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 7, 2025

|time=7:00 p.m. CDT

|team1=FC Dallas {{flagicon|Texas}}

|score=

|report=

|team2={{flagicon|California}} AV Alta FC

|goals1=

|goals2=

|stadium=Toyota Stadium

|location=Frisco, Texas

|attendance=

|referee=

}}

Kasey Keller Group

{{footballbox collapsible

|date=May 6, 2025

|time=7:00 p.m. PDT

|team1=Tacoma Defiance {{flagicon|Washington}}

|score=

|report=

|team2={{flagicon|Oregon}} Portland Timbers

|goals1=

|goals2=

|stadium=Starfire Sports Complex

|location=Tukwila, Washington

|attendance=

|referee=

}}

{{footballbox collapsible

|date=May 7, 2025

|time=7:30 p.m. PDT

|team1=San Jose Earthquakes {{flagicon|California}}

|score=

|report=

|team2={{flagicon|California}} Sacramento Republic FC

|goals1=

|goals2=

|stadium=PayPal Park

|location=San Jose, California

|attendance=

|referee=

}}

The 2025 U.S. Open Cup winner earns a place in the 2026 CONCACAF Champions Cup.

{{updated|April 16, 2025}}.

{{legend|silver|Team eliminated for this round.}}

{{legend|#DDDDDD|Team was inactive for this round.}}

TNT Sports holds the rights to broadcast the Open Cup from 2023 to 2030, as part of a larger media rights agreement with U.S. Soccer.{{cite press release |date=March 1, 2022 |title=Turner Sports and United States Soccer Federation Reach Multimedia Rights Agreement |url=https://www.ussoccer.com/stories/2022/03/turner-sports-and-united-states-soccer-federation-reach-multimedia-rights-agreement |publisher=United States Soccer Federation |access-date=March 22, 2022}}{{Cite web|url=https://worldsoccertalk.com/2022/02/15/cindy-parlow-cone-talks-us-soccer-media-rights/|title=Cindy Parlow Cone talks US Soccer media rights|first=Kartik|last=Krishnaiyer|date=February 15, 2022}} The U.S. Soccer YouTube channel will broadcast all games from the first two rounds. On March 26, 2025, CBS Sports announced an agreement to exclusively broadcast all games beginning with the third round. All games will stream on Paramount+, with select games on the CBS Sports Golazo Network and CBS Sports Network.{{cite press release |date=March 26, 2025 |title=U.S. SOCCER AND CBS SPORTS REACH MULTIMEDIA RIGHTS AGREEMENT FOR 2025 & 2026 LAMAR HUNT U.S. OPEN CUP TOURNAMENTS |url=https://www.paramountpressexpress.com/cbs-sports/releases/?view=111224-us-soccer-and-cbs-sports-reach-multimedia-rights-agreement-for-2025-2026-lamar-hunt-us-open-cup-tournaments |publisher=CBS Sports |access-date=March 26, 2025}}

{{reflist}}

• {{official website|https://www.ussoccer.com/us-open-cup}}

{{U.S. Open Cup}}

{{2025 in American men's soccer}}

Category:U.S. Open Cup

Cup

US Open Cup