List of animated series with LGBTQ characters: 2000–2004

{{For-text|the other lists of LGBTQ characters|List of animated series with LGBTQ characters|the history of LGBTQ themes and characters in Western animation|LGBTQ themes in Western animation|the history of LGBTQ themes and characters in anime|LGBTQ themes in anime and manga}}

The number of animated series with LGBTQ characters increased from the previous decade. From 2000 to 2004, such characters appeared prominently in Queer Duck, Drawn Together, and The Venture Bros. all of which were Western animations. However, they appeared more frequently in anime series such as Gravitation, Kino's Journey, Kannazuki no Miko, and My-HiME. The shows airing during this period set the stage for those to come in 2005 to 2009, the latter part of the decade.

This list only includes recurring characters, otherwise known as supporting characters, which appear frequently from time to time during the series' run, often playing major roles in more than one episode, and those in the main cast are listed below. LGBTQ characters which are guest stars or one-off characters are listed on the pages focusing exclusively on gay (in animation and anime), lesbian (in animation and anime), bisexual (in animation and anime), trans, pansexual, asexual, non-binary, and intersex characters.

The entries on this page are organized alphanumerically by duration dates and then alphabetically by the first letter of a specific series.

2000

class="wikitable sortable" style="width: 100%"

!scope="col" style="width:10%;"| Duration

!scope="col" style="width:10%;"| Show title

!scope="col" style="width:12%;"| Character debut date

!scope="col" style="width:12%;"| Characters

!scope="col" style="width:12%;"| Identity

! Notes

! Country

rowspan="2" | 2000

| rowspan="2"|Descendants of Darkness

| rowspan="2"|{{sort|2000/10/02|October 2, 2000}}

| Asato Tsuzuki

| Gay

| He is a god of death, who works at the Summons Department, and is Hisoka's partner.{{cite web|url=http://www.kingrecords.co.jp/SCR_CATALOG/034.html|title=Descendants of Darkness|website=King Records|archive-url=https://web.archive.org/web/20060630175001/http://www.kingrecords.co.jp/SCR_CATALOG/034.html|archive-date=June 30, 2006|access-date=September 17, 2021|url-status=dead}} He begins to fall in love with Hisoka, and Hisoka with him, from the first episode of the series.{{cite book |last=Solomon |first=Charles |title=Anime, mon amour |publisher=The Advocate |date=October 14, 2003 |pages=86, 88 |url=https://books.google.com/books?id=RGUEAAAAMBAJ&pg=PA86}}

| rowspan="2" |Japan

Hisoka Kurosaki

| Bisexual

| Also a god, but with strong psychic abilities, he partners up with Asato, who didn't want anyone to be his partner.{{cite web|url=http://www.jcstaff.co.jp/sakuhin/nenpyo/2000/02_yami/yami.htm|title=オフィシャルホームページ闇の末裔|trans-title=Official homepage Descendants of Darkness|website=J.C. Staff|access-date=September 17, 2021|archive-url=https://web.archive.org/web/20210321220109/http://www.jcstaff.co.jp/sakuhin/nenpyo/2000/02_yami/yami.htm|archive-date=March 21, 2021|url-status=live}} Other than his love for Asato, Hisoka showed some level of feelings for a girl named Tsubaki Kakyouin.

rowspan="3"|2000–2001

| For Better or For Worse

| November 12, 2000

| Lawrence Poirier

| Gay

| Lawrence Poirier has been one of Michael Patterson's friends since childhood. In the episode "Parade Passing By", he participates in the gay pride parade.{{cite web|url=https://www.metacritic.com/tv/for-better-or-for-worse-2000/season-1/episode-9-family-lies|title=FAMILY LIES For Better or for Worse (2000): Season 1: Episode 9|website=Metacritic|access-date=December 23, 2021}}{{better source needed|date=July 2024}} Lawrence came out as gay in the comic strip in 1993.{{cite news |url=http://www.cbc.ca/national/blog/video/arts/life_in_a_comic_strip.html |archive-url=https://web.archive.org/web/20080106120730/http://www.cbc.ca/national/blog/video/arts/life_in_a_comic_strip.html |archive-date=2008-01-06 |title=CBC: Life is a comic strip |date=January 6, 2008 |access-date=January 19, 2012}} He also appeared in the animated specials that were released between 1985 and 1995.

| Canada

rowspan="2"|Gravitation

| rowspan="2"|{{sort|2000/10/04|October 4, 2000}}

| Shuichi Shindo

| Gay

| A vocalist, he has a chance encounter with Eiri Yuki early in the series.{{cite web|url=https://www.aniplex.co.jp/gravi/character/index.html|title=TVアニメーショングラビテーション|trans-title=TV Animation Gravitation|website=Aniplex|access-date=September 17, 2021|archive-url=https://web.archive.org/web/20210318072436/https://www.aniplex.co.jp/gravi/character/index.html|archive-date=March 18, 2021|url-status=live}} He falls in love with Eiri Yuki beginning in the first episode and over the course of the series manages to melt the cold heart of Eiri, with the two becoming a couple.{{Sfn | University of the Pacific | 2004 | p = 10-11, 17}}{{cite web|last1=Hutchinson|first1=Sam|title=10 LGBTQ+ Anime You Need To Watch|url=https://www.cbr.com/must-watch-lgbtq-anime/|archive-url=https://web.archive.org/web/20200807010642/https://www.cbr.com/must-watch-lgbtq-anime/|url-status=live|archive-date=August 7, 2020|website=CBR|date=August 1, 2020|access-date=August 6, 2020}}

| rowspan="2"|Japan

Eiri Yuki

| Bisexual

| A man with a mysterious past, he is a romance novelist whose book is "popular with female fans," but becomes interested in Shuichi. In one episode, "Winding Road," it is revealed that he has a fiancée named Ayaka Usami, but she backs away after seeing how much he loves Shuichi.{{cite web |last1=Solomon |first1=Charles |title='Gravitation': Boy-Meet-Boy Anime Made for Teen Girls |url=https://www.animationmagazine.net/anime/gravitation-boy-meet-boy-anime-made-for-teen-girls/ |website=Animation Magazine |access-date=July 13, 2020 |archive-url=https://web.archive.org/web/20191205033514/https://www.animationmagazine.net/anime/gravitation-boy-meet-boy-anime-made-for-teen-girls/ |archive-date=December 5, 2019 |date=November 22, 2019 |url-status=live |df=mdy-all }}

2000-2002

| Gotham Girls

| 2002

| Selma Reesedale

| Trans woman

| Selma Reesedale works for the Gotham PD. She is revealed to be a transgender woman, which Batgirl discovers, and she later helps Batgirl.{{cite web|url=https://dcanimated.com/WF/gothamgirls/bios/rees/|title=Bios - Selma Reesdale|work=The World's Finest|access-date=February 4, 2022|archive-url=https://web.archive.org/web/20220204223714/https://dcanimated.com/WF/gothamgirls/bios/rees/|archive-date=February 4, 2022|url-status=live}}{{cite web|last=Rude|first=Mey|url=https://www.autostraddle.com/supergirls-like-us-batgirls-alysia-yeoh-is-trans-172660/|title=Supergirls Like Us: Batgirl's Alysia Yeoh Is Trans|website=Autostraddle|date=April 11, 2013|access-date=February 4, 2022|archive-url=https://web.archive.org/web/20210302171936/https://www.autostraddle.com/supergirls-like-us-batgirls-alysia-yeoh-is-trans-172660/|archive-date=March 2, 2021|url-status=live}} Batgirl is one of the only characters who knows Selma's secret.{{cite web|author=J. Skyler|url=http://www.comicbookbin.com/Gail_Simone_to_Write_Trans_001.html|title=Gail Simone to Write Transgender Character|website=Comic Book Bin|date=January 1, 2013|archive-url=https://web.archive.org/web/20210212012156/http://www.comicbookbin.com/Gail_Simone_to_Write_Trans_001.html|archive-date=February 12, 2021|url-status=live}} She is possibly the first trans sci-fi character to appear in any "superhero production."{{cite web |last=Stevens |first=Geoffrey |title=Gotham Girls |url=https://sf-encyclopedia.com/entry/gotham_girls |magazine=The Encyclopedia of Science Fiction |access-date=February 4, 2022 |archive-url=https://web.archive.org/web/20210303032916/https://sf-encyclopedia.com/entry/gotham_girls |archive-date=March 3, 2021 |date=June 24, 2019 |url-status=live}}

| United States

rowspan="2"|2000-2003

| rowspan="2"|X-Men: Evolution

| November 4, 2000

| Mystique

| rowspan="2"|Bisexual

| rowspan="2"|Character designer and show director Steven E Gordon confirmed Mystique and Destiny were lovers similar to the comics.{{Cite web|last=Zachary|first=Brandon|url=https://www.cbr.com/x-men-evolution-mystique-mcu-blueprint/|title=X-Men: Evolution's Mystique Can Be the Blueprint For the MCU Version|website=Comic Book Resources|date=August 22, 2022|access-date=January 6, 2023}} Mystique and Destiny are both known for being in a long-time same-sex relationship in the comics. They are also attracted to both men and women.{{Cite web |last=Dry |first=Jude |date=July 25, 2019 |title=Marvel's LGBTQ+ Superheroes: The 8 Most Likely Candidates to Queer the MCU |url=https://www.indiewire.com/gallery/gay-marvel-superhero-lgbt-mcu/ |access-date=August 28, 2022 |website=IndieWire |language=en}}{{better source needed|date=July 2024}}

| rowspan="2"|United States

November 18, 2000

| Destiny

rowspan="5"|2000–2004

| Inuyasha

| February 24, 2003

| Jakotsu

| Gay

| Jakotsu is a member of the Jakotsutō, a criminal band of seven assassins. He is shown as being flamboyant, playful, and narcissistic, even liking Inuyasha, pursuing him as prey.{{cite web|url=https://www.sunrise-inc.co.jp/yasya/chara/character_04.html|title=犬夜叉 official web - 登場人物紹介 [七人隊]|trans-title=Inuyasha official web --Character introduction [Seven people]|website=Sunrise|access-date=September 17, 2021|archive-url=https://web.archive.org/web/20201107085514/https://www.sunrise-inc.co.jp/yasya/chara/character_04.html|archive-date=November 7, 2020|url-status=live}} He also has a strong preference for men, complimenting Koga's loin cloth and Sesshomaru's appearance, and flirting with Miroku.{{cite book |last=Robertson |first=Mary |author-link=Mary Robertson |date=2018 |title=Growing Up Queer: Kids and the Remaking of LGBTQ Identity |url=https://books.google.com/books?id=uXR0DwAAQBAJ&pg=PA107 |location=New York City |publisher=New York University Press |pages=107–108 |isbn=9781479876945}}

| Japan

rowspan="3"|Queer Duck

| rowspan="2"|October 11, 2000

| Adam Seymour Duckstein

| Gay

| Adam is the main character of the series. He is presented as a stereotypical gay duck{{Cite web |author=Multi Channel News Staff |title=Queer Duck Crosses the Pond |url=https://www.multichannel.com/news/queer-duck-crosses-pond-163975 |website=Multichannel News |access-date=July 10, 2020 |archive-url=https://web.archive.org/web/20200710131107/https://www.multichannel.com/news/queer-duck-crosses-pond-163975 |archive-date=July 10, 2020 |date=March 29, 2018 |url-status=live |df=mdy-all }}{{cite web|url=https://iceboxanimation.com/?p=385|title=Queer Duck Episode 1: "I'm Coming Out!"|website=icebox.com|access-date=September 17, 2021|date=January 9, 2013|archive-url=https://web.archive.org/web/20210917224749/https://iceboxanimation.com/?p=385|archive-date=September 17, 2021|url-status=live}} and has been a victim of gay bashing.{{Cite web |last1=Meisler |first1=Andy |title=Television/Radio; 'Queer Duck,' a Web-Footed Survivor, Migrates to TV |url=https://www.nytimes.com/2002/04/07/arts/television-radio-queer-duck-a-web-footed-survivor-migrates-to-tv.html |website=New York Times |access-date=April 25, 2020 |archive-url=https://web.archive.org/web/20200325230440/https://www.nytimes.com/2002/04/07/arts/television-radio-queer-duck-a-web-footed-survivor-migrates-to-tv.html |archive-date=March 25, 2020 |date=April 7, 2002 |url-status=live |df=mdy-all }} However, in the series finale, he wakes up to discover that he had sex with a woman. Queer Duck is voiced by gay actor and comedian, Jim J. Bullock.{{cite web|title=Phase Two HIV-Positive Actor and Comedian Jim J. Bullock Takes a Shot at the Stage|url=http://www.danndulin.com/jimjbullock.html|website=Dan Dulin|access-date=December 28, 2021}}

| rowspan="3"|United States

Steven Arlo Gator

| Gay

| Steven is Queer Duck's significant other. He is shy and insecure and is usually the voice of reason whenever Queer Duck gets himself or anyone else in trouble. He marries Queer Duck in a Jewish Wedding in Vermont in one episode, "Wedding Bell Blues," (although they are often seen as having an open relationship); a moose was the rabbi.{{citation needed|date=July 2024}}

April 24, 2002

| Melissa Duckstein

| Lesbian

| Melissa is Adam's sister. She is occasionally revealed to be lesbian throughout the series,{{cite book |last=Terrace |first=Vincent |date=2015 |title=Internet Lesbian and Gay Television Series, 1996-2014 |url=https://books.google.com/books?id=-EW7CQAAQBAJ&pg=PA152 |location=Jefferson, North Carolina |publisher=McFarland & Company |page=152 |isbn=978-0786498055}} in a relationship with Yvette, in episodes such as "Homo for the Holidays," and the movie.

Static Shock

| September 23, 2000

| Richard "Richie" Osgood Foley

| Gay

| Best friend of Static/Virgil, Richie, also known as Gear, was based on an openly gay character named Rick Stone from the original comic. Dwayne McDuffie, one of the show's creators, said he dealt with the homosexuality of Richie by writing him "aggressively and unconvincingly announcing his heterosexuality whenever possible...while Virgil rolled his eyes at the transparency of it" but it never came up in the show because it was rated TV-Y7-FV.{{cite web|last=Dar|first=Taimur|url=https://www.comicsbeat.com/marvels-earliest-gay-characters-introduced-in-don-mcgregors-black-panther-comics/|title=Marvel's Earliest Gay Characters Introduced in Don McGregor's Black Panther Comics|website=ComicsBeat|date=February 12, 2018|access-date=June 10, 2021|archive-url=https://web.archive.org/web/20210128094954/https://www.comicsbeat.com/marvels-earliest-gay-characters-introduced-in-don-mcgregors-black-panther-comics/|archive-date=January 28, 2021|url-status=live}}

| United States

rowspan="4"|2000-2007

| rowspan="4"|Harvey Birdman, Attorney at Law

| rowspan="3"|December 30, 2000

| Harvey Birdman

| Bisexual

| Harvey Birdman has shown attraction to men and women. He had a same-sex relationship with Boo-Boo Bear and married him in the season 3 episode "Return of Birdgirl".{{cite web|url=https://www.avclub.com/10-episodes-of-harvey-birdman-attorney-at-law-to-watch-1829477536|title=10 episodes of Harvey Birdman, Attorney At Law to watch before the new special|first=Sam|last=Barsanti|website=The A.V. Club|date=October 11, 2018|access-date=December 24, 2022}}

| rowspan="4"|United States

Dr. Benton Quest

| rowspan="3"|Gay

| rowspan="2"|Dr. Benton Quest and Race Bannon are portrayed as a same-sex couple in the series. The first episode "Bannon Custody Battle" is about Race Bannon wanting to share custody of Jonny and Hadji with Benton though it's revealed in court that Race Bannon is a robot duplicate created by Dr. Zin and the real Race Bannon was on vacation.{{cite web|url=https://www.escapistmagazine.com/remembering-adult-swim-classic-harvey-birdman/|title=Remembering Adult Swim Classic Harvey Birdman|first=Bob|last=Chipman|website=The Escapist|date=July 28, 2014|access-date=June 9, 2022}} The season 3 episode "Return of Birdgirl" dealt with same-sex marriage as Dr. Quest and Race wanted to get legally married.

Race Bannon

July 14, 2002

| Boo-Boo Bear

| Boo-Boo Bear is portrayed as Harvey's gay lover in the series.

2001

class="wikitable sortable" style="width: 100%"

!scope="col" style="width:10%;"| Duration

!scope="col" style="width:10%;"| Show title

!scope="col" style="width:12%;"| Character debut date

!scope="col" style="width:12%;"| Characters

!scope="col" style="width:12%;"| Identity

! Notes

! Country

rowspan="4"|2001

| Fruits Basket

| September 6, 2001

| Hatsuharu Sohma

| Bisexual

| Hatsuharu is part of the Sohma family, a family cursed into turning into members of the zodiac when under stress or hugged by members of the opposite sex. He was born in the year of the cow and was often annoyed by people's comments about the stupidity of the cow from the zodiac, and blamed his troubles on Yuki who was born the year of the rat.{{cite web |title=Yuki Soma |url=https://www.fruitsbasketofficial.com/characters/yuki-soma |website=Fruits Basket official website |publisher=Funimation |access-date=July 14, 2020 |archive-url=https://archive.today/20200715003053/https://www.fruitsbasketofficial.com/characters/yuki-soma/ |archive-date=July 15, 2020 |date=2020 |url-status=live |df=mdy-all }} However, Yuki helps him realize he needs to move past people's comments and enters an on/off relationship with Rin Sohma, who dumped him in Season 2,{{cite news |first1=Kemner |last1=Louis |title=Fruits Basket: Hatsuharu & Yuki Fight Back Against the Sohma Curse |url=https://www.cbr.com/fruits-basket-hatsuharu-yuki-curse/ |website=CBR |access-date=July 14, 2020 |archive-url=https://web.archive.org/web/20200527175226/https://www.cbr.com/fruits-basket-hatsuharu-yuki-curse/ |archive-date=May 27, 2020 |date=May 2, 2020 |url-status=live |df=mdy-all }} he refers to Yuki as his first love.{{cite web |first1=Anthony |last1=Gramuglia |title=Fruits Basket: The Sohma Family Has Another Curse - and It's Gross |url=https://www.cbr.com/fruits-basket-sohma-curse-incest/ |website=CBR |access-date=July 14, 2020 |archive-url=https://web.archive.org/web/20200430024714/https://www.cbr.com/fruits-basket-sohma-curse-incest/ |archive-date=April 30, 2020 |date=April 18, 2020 |url-status=live |df=mdy-all }}{{cite web |title=Hatsuharu Soma |url=https://www.fruitsbasketofficial.com/characters/hatsuharu-soma |website=Fruits Basket official website |access-date=July 14, 2020 |archive-url=https://archive.today/20200715003328/https://www.fruitsbasketofficial.com/characters/hatsuharu-soma/ |archive-date=July 15, 2020 |date=2020 |url-status=live |df=mdy-all }}

| Japan

Puni Puni Poemy

| March 7, 2001

| Futaba Aasu

| Lesbian

| Poemy's classmate and best friend, and she is madly in love with her, a fact to which Poemy is oblivious.{{cite web |author=Christi |title=Puni Puni Poemy [review] |url=https://www.themanime.org/viewreview.php?id=320 |website=THEM Anime Reviews |access-date=June 30, 2020 |archive-url=https://web.archive.org/web/20190725142422/https://www.themanime.org/viewreview.php?id=320 |archive-date=July 25, 2019 |date=2001 |url-status=live |df=mdy-all }}{{cite web|url=http://www.jvcmusic.co.jp/flyingdog/poemi/story/index.html|title=ぷにぷに☆ぽえみぃ Story|trans-title=Puni Puni ☆ Poemii Story|website=Official Puni Puni Poemy website|publisher=VICTOR ENTERTAINMENT, INC.|access-date=September 21, 2021|archive-url=https://web.archive.org/web/20200128032613/http://www.jvcmusic.co.jp/flyingdog/poemi/story/index.html|archive-date=January 28, 2020|url-status=live}} In the final confrontation with the invading aliens, in the second episode, Futaba combines her power with Poemy's, and since Poemy is a representation of Earth, this act pacifies the planet's population, resulting in peace with the aliens.{{cite web |last1=Turner |first1=Sam |title=Puni Puni Poemi DVD [review] |url=https://www.animenewsnetwork.com/review/puni-puni-poemi/dvd |website=Anime News Network |access-date=June 30, 2020 |archive-url=https://web.archive.org/web/20200111051926/https://www.animenewsnetwork.com/review/puni-puni-poemi/dvd |archive-date=January 11, 2020 |date=September 2, 2004 |url-status=live |df=mdy-all }} After this event, director Nabeshin casually mentions that Futaba is in reality the main character of the show. Her name comes from futa, the Japanese word for "two". Futaba is mostly a parody of Tomoyo Daidouji, the best friend of magical girl Sakura Kinomoto from Cardcaptor Sakura who is also a lesbian. Unlike Tomoyo, however, Futaba displays more extreme fantasies and lesbian arousal whenever she is with her friend, who she seriously loves.{{cite web|url=http://www.jvcmusic.co.jp/flyingdog/poemi/chara/index.html|title=ぷにぷに☆ぽえみぃ [Character]|trans-title=Puni Puni ☆ Poemii [Character]|website=Official Puni Puni Poemy website|publisher=VICTOR ENTERTAINMENT, INC.|access-date=September 21, 2021|archive-url=https://web.archive.org/web/20210126124639/http://www.jvcmusic.co.jp/flyingdog/poemi/chara/index.html|archive-date=January 26, 2021|url-status=live}}

| Japan

rowspan="2"|Zoids: New Century

| rowspan="2"|January 20, 2001

| Benjamin

| rowspan="2"|Gay

| rowspan="2"|Benjamin and Sebastian are Harry Champ's robots. They are the only other members of the Champ Team. Both of the robots are indicated to be gay in the Japanese version. In the episode "My Love, the Judge", Benjamin is shown to be in love with a male Judge Robot. This was censored in the English dub as the Judge Robot was changed to a female Judge. In the Japanese version of "In Search of the Ultimate X", both Benjamin and Sebastian were wearing bikinis and rubbing each other. Those scenes were cut in the English dub.{{cite web|url=http://mbs.jp/zero/|title=[ストーリー]|trans-title=[Story]|website=Official Zoids: New Century MBS website|publisher=MBS|access-date=December 27, 2021|archive-url=https://web.archive.org/web/20020221144026/http://mbs.jp/zero/|archive-date=February 21, 2002|url-status=dead}}

| rowspan="2"|Japan

Sebastian

rowspan="6"|2001–2002

| rowspan="2"|John Callahan's Quads!

| rowspan="2"|February 2, 2001

| rowspan="2"|Spalding

| rowspan="2"|Gay

| rowspan="2"|Spalding is Reilly's Australian physical therapist. He is a drama queen and openly gay.{{cite web|url=https://www.mediaworld.com.au/quads|title=John Callahan's QUADS!|work=Media World|access-date=July 5, 2021|archive-url=https://web.archive.org/web/20210311141232/https://www.mediaworld.com.au/quads|archive-date=March 11, 2021|url-status=live}}{{cite web|last=Rose|first=Damon|url=https://www.bbc.co.uk/blogs/ouch/2005/04/callahans_quads_reach_uk.html|title=Callahan's Quads reach UK|website=BBC|date=April 11, 2005|access-date=September 21, 2021|archive-url=https://web.archive.org/web/20180602083509/https://www.bbc.co.uk/blogs/ouch/2005/04/callahans_quads_reach_uk.html|archive-date=June 2, 2018|url-status=live}} The last two episodes of season 1, "Fatal Distraction" and "Spalding in the Family Way" centered on Spalding as he tries to get back together with his former boyfriend Bruce and coming out to his family.

|Canada

Australia

rowspan="2"|The Oblongs

|rowspan="2"|April 2, 2001

|Biff Oblong

|Gay

|Biff Oblong was indicated to be gay when he showed attraction to a male coach.{{cite web|last=Howe|first=Nicholas|url=https://screenrant.com/oblings-jokes-not-funny-outdated/|title=10 Jokes From The Oblongs That Have Already Aged Poorly|website=Screen Rant|date=September 28, 2019|access-date=September 21, 2021|archive-url=https://archive.today/20210922020514/https://screenrant.com/oblings-jokes-not-funny-outdated/|archive-date=September 22, 2021|url-status=live}} He was confirmed to be gay in a bonus feature from The Oblongs Complete Series DVD.{{cite AV media|date=October 4, 2005|title=The Oblongs - The Complete Series|trans-title=|medium=DVD |language=en|url=https://www.amazon.com/Oblongs-Complete-Mike-Kim/dp/B000ABKHZC|access-date=February 4, 2022|format=DVD|location=US|publisher=Warner Brothers|id=B000ABKHZC}}

|rowspan="2"|United States

Anita Bidet

|Trans woman

|Anita Bidet is the owner of the bar Rusty Bucket and Pickles Oblong's friend. She is a pre-op transgender woman who is saving up money for gender reassignment surgery.{{cite web |last1=Anderson-Minshall |first1=Diane |title=How Animated Shows Like Futurama Can Unite Us All |url=https://www.advocate.com/commentary/2013/06/18/op-ed-how-animated-shows-futurama-can-unite-us-all |website=The Advocate |access-date=April 26, 2020 |archive-url=https://web.archive.org/web/20160312102656/http://www.advocate.com/commentary/2013/06/18/op-ed-how-animated-shows-futurama-can-unite-us-all |archive-date=March 12, 2016 |date=June 18, 2013 |url-status=live |df=mdy-all }} The series creator called her a "drag queen."{{cite web|last=Oblong|first=Angus|url=https://www.angusoblong.com/products/james-development-art-from-the-oblongs-1999|title=James Development Art from The Oblongs. 1999.|website=official Angus Oblong website|access-date=September 21, 2021|archive-url=https://web.archive.org/web/20200813135744/https://www.angusoblong.com/products/james-development-art-from-the-oblongs-1999|archive-date=August 13, 2020|url-status=live}}{{cite magazine |last=Graham |first=Chris |date=April 24, 2001 |title=An Oblong look at the world |url=https://books.google.com/books?id=xmIEAAAAMBAJ&pg=PA58 |magazine=The Advocate |page=58 |location=Los Angeles, California |publisher=Here Publishing |access-date=September 21, 2021 |issn=}}

rowspan="2"|X

| August 25, 2001

| Subaru Sumeragi

| rowspan="2"|Gay

| rowspan="2"|Subaru tries to hunt and kill Seishirō for killing his sister and when he succeeds, he states he has killed the one he loves most.{{cite web |last1=Bertschy |first1=Zac |title=X TV DVD 3 [Review] |url=https://www.animenewsnetwork.com/review/x-tv/dvd-3 |website=Anime News Network |access-date=July 14, 2020 |archive-url=https://web.archive.org/web/20190608025215/https://www.animenewsnetwork.com/review/x-tv/dvd-3 |archive-date=June 8, 2019 |date=April 2, 2003 |url-status=live |df=mdy-all }} Their past is elaborated in the manga, Tokyo Babylon, where it was shown Seishirō would often flirt with and tried to seduce Subaru as part of a bet: if Subaru could make him fall in love with him, he wouldn't kill him. While Subaru did grow to love Seishirō, Seishirō couldn't reciprocate but killed Subaru's sister Hotaru in his stead per her request, and as Seishirō dies, he confesses "I also.." before dying.{{cite web | title=X Vol. No. 6 | publisher=Mania Entertainment |date=2 August 2003|author=Beveridge, Chris| accessdate=July 14, 2020|url=http://www.mania.com/x-vol-6_article_75271.html |archive-url=https://web.archive.org/web/20150402101539/http://www.mania.com/x-vol-6_article_75271.html|archive-date=April 2, 2015|url-status=dead}}

|rowspan="2"|Japan

October 3, 2001

| Seishirō Sakurazuka

2001–2003

| Time Squad

| June 8, 2001

| Larry 3000

| Gay

| Larry 3000's voice actor, Mark Hamill, implied{{cite web |title=039 – Mark Hamill on Talkin Toons with Rob Paulsen – Weekly Voice Acting and Voice Over Tips |url=http://techjives.net/2012/05/04/039-mark-hamill-on-talkin-toons-with-rob-paulsen-weekly-voice-acting-and-voice-over-tips/ |website=Tech Jives Network |access-date=March 18, 2020 |archive-url=https://web.archive.org/web/20200319040214/http://techjives.net/2012/05/04/039-mark-hamill-on-talkin-toons-with-rob-paulsen-weekly-voice-acting-and-voice-over-tips/ |archive-date=March 19, 2020}} that Larry could easily have been interpreted as gay in the cartoon, due to his femininity and presentation as the "gay best friend" to Cleopatra in "Shop like an Egyptian", although Larry has stated on multiple occasions he dislikes humans in general. However, this show never stated his sexuality, although Hamill described Lary 3000 as fierce and flamboyant.{{cite web |title=Love Unique Deadpan No-Nonsense #Skips but #Larry3000 of #TimeSquad was Fierce-Flamboyant & more Crazy-Fun to play! |url=https://twitter.com/HamillHimself/status/733579727215042560 |website=Twitter |publisher=Mark Hamill |access-date=March 18, 2020|archive-url=https://archive.today/20200319034956/https://twitter.com/HamillHimself/status/733579727215042560 |archive-date=March 19, 2020}}

| United States

2001–2004

| Braceface

| September 27, 2002

| Mark "Dion" Jones

| Gay

| Canon gay male character as shown in the episode "Game, Set-up and Match." Sharon even tried to set Dion up for a date but it didn't work out.{{cite web|last=Bell|first=Crystal|url=http://www.mtv.com/news/2225169/sharon-spitz-braceface/|title=11 Times You Felt Mortified For Sharon Spitz On 'Braceface'|work=MTV|date=28 July 2015|access-date=August 6, 2015|archive-url=https://web.archive.org/web/20190217011430/http://www.mtv.com/news/2225169/sharon-spitz-braceface/|archive-date=February 17, 2019 }} The show's official website called him "sweet, flamboyant and funny."{{cite web|url=http://bracefacetv.com/characters.htm|title=Characters|website=Braceface|publisher=Nelvana Limited|access-date=September 21, 2021|archive-date=September 20, 2013|archive-url=https://web.archive.org/web/20130920190224/http://bracefacetv.com/characters.htm|url-status=live}}

| Canada

2002

class="wikitable sortable" style="width: 100%"

!scope="col" style="width:10%;"| Duration

!scope="col" style="width:10%;"| Show title

!scope="col" style="width:12%;"| Character debut date

!scope="col" style="width:12%;"| Characters

!scope="col" style="width:12%;"| Identity

! Notes

! Country

rowspan="5"|2002

| Azumanga Daioh

| May 13, 2002

|Kaorin

| Lesbian

| Kaorin has unrequited love for the athletic girl, Sakaki, described as totally idolizing her, and is horrified that Mr. Kimura likes her.{{cite web|url=http://www.advfilms.com/titles/azumanga/yearbook/supp.php|title=Yearbook - Supporting Characters|website=Azumanga Daioh|publisher=ADV Films|archive-url=https://web.archive.org/web/20040616110431/http://www.advfilms.com/titles/azumanga/yearbook/supp.php|archive-date=June 16, 2004|url-status=dead}}{{cite web|url=http://www.advfilms.com/titles/azumanga/yearbook/sakaki.php|title=Yearbook - Sasaki|website=Azumanga Daioh|publisher=ADV Films|archive-url=https://web.archive.org/web/20040616110431/http://www.advfilms.com/titles/azumanga/yearbook/supp.php|archive-date=June 16, 2004|url-status=live}} Osaka specifically asks Kaori if she is homosexual, Kaori becomes embarrassed and then answers that it's "one of those things peculiar to puberty" and that she'd love Sakaki if say, she 'were' male or whatever, because she's cool.Azuma, Kiyohiko (July 2009). Azumanga Daioh: Supplementary Lessons. Monthly Shōnen Sunday (in Japanese). 3. Japan: Shogakukan. p. 119.{{better source needed|date=July 2024}} In another translation, she yells "the current term is lesbian!" before denying she is a lesbian.{{efn|In Azumanga Daioh: Supplementary Lessons Chapter 3 Page 12, Osaka asks Kaorin whether she is gay, to which she responds "The correct term is Lesbian!" before denying that she is a lesbian.}} Others say the show makes her "overtly gay."{{Cite web|last1=Bryant|first1=Lauren|url=https://artduckomagazine.wordpress.com/2018/04/12/revisiting-azumanga-daoioh-by-lauren-bryant/|title=Revisiting "Azumanga Daoioh"|date=April 12, 2018|website=Art Decko Magazine|language=en|access-date=July 14, 2020|archive-url=https://archive.today/20200715020042/https://artduckomagazine.wordpress.com/2018/04/12/revisiting-azumanga-daoioh-by-lauren-bryant/|archive-date=July 15, 2020}}

| Japan

rowspan="2"|.hack//Sign

| rowspan="2"|April 4, 2002

| An Shoji (Tsukasa)

| rowspan="2"|Lesbian

| She plays as a male character online due to the mental and physical abuse she suffers from her father, as she hoped that by playing as a male avatar she could distance herself from real life.{{Cite web|last1=Dong|first1=Bamboo|url=https://www.animenewsnetwork.com/review/.hack-sign/dvd-1|title=.hack//SIGN Limited Edition DVD 1: Login [Review]|date=March 15, 2003|website=Anime News Network|language=en|access-date=July 14, 2020|archive-url=https://web.archive.org/web/20190303074004/https://www.animenewsnetwork.com/review/.hack-sign/dvd-1|archive-date=March 3, 2019}} As Tsukasa (her male avatar), she meets and falls in love with Subaru, the avatar of Mariko Misono, indicated in episodes like "Encounter." The two girls later make plans to meet in real life in the episode "Net Slum."{{Cite web|last1=Creamer|first1=Nick|url=https://www.animenewsnetwork.com/review/hack/sign/dvd-complete-series/.88373|title=.hack//SIGN DVD - Complete Series [Review]|date=May 21, 2015|website=Anime News Network|language=en|access-date=July 14, 2020|archive-url=https://web.archive.org/web/20190310002522/https://www.animenewsnetwork.com/review/hack/sign/dvd-complete-series/.88373|archive-date=March 10, 2019}}

| rowspan="2"|Japan

Mariko Misono (Subaru)

| Subaru falls in love with An Shoji (Tsukasa), and both girls later make plans to meet in real life in the episode "Net Slum."

rowspan="2"|Mirage of Blaze

| rowspan="2"|January 7, 2002

| Takaya Ougi

| rowspan="2"|Gay

| He is a high school student in Matsumoto, and the reincarnation of Kagetora Uesugi.{{Cite web|url=https://www.aniplex.co.jp/mirage/intro/index.html|title=Introduction|website=Mirage of Blaze official website|publisher=Aniplex|access-date=July 14, 2020|archive-url=https://web.archive.org/web/20190709194105/https://www.aniplex.co.jp/mirage/intro/index.html|archive-date=July 9, 2019|language=ja}}{{Cite web|url=https://www.aniplex.co.jp/mirage/story/index.html|title=Story TV Series|website=Mirage of Blaze official website|publisher=Aniplex|access-date=September 26, 2021|archive-url=https://web.archive.org/web/20201018202626/https://www.aniplex.co.jp/mirage/story/index.html|archive-date=October 18, 2020|language=ja}} Kagetora was raped by his vassals and pages and so he and his reincarnation Takaya are androphobic and hate men, but Takaya begins to fall in love with Yoshiaki/Naoe, who is persistent in his advances.

| rowspan="2"|Japan

Yoshiaki Tachibana

| He is the reincarnation of Nobutsuna Naoe.

2002–2003

|Cheeky Angel

|June 4, 2002

| Megumi Amatsuka

| Trans woman

|Megumi is a physically attractive trans woman who retains her masculine mannerisms and fighting abilities, which she uses very often, attracting the 'Megu-chan Protection Club', a group of misfit admirers.{{Cite web|url=https://www.viz.com/cheeky-angel|title=Cheeky Angel|website=VIZ Media|language=en|access-date=August 26, 2021|archive-url=https://web.archive.org/web/20190404142804/https://www.viz.com/cheeky-angel|archive-date=April 4, 2019|url-status=dead}}{{cite web|url=http://viz.com/product?id=1192|title=Cheeky Angel Vol. 1|website=VIZ Media|language=en|archive-url=https://web.archive.org/web/20111216194828/http://viz.com/product?id=1192|archive-date=December 16, 2011|url-status=dead}} Nobody else knows she used to be a boy but was transformed into a girl. Initially, only Megumi's best friend, Miki, knew her secret, however the protection club quickly finds out.{{Cite web|author=Arevalo, J.P.|url=https://www.animefringe.com/magazine/02.08/feature/6/index.php3|title=Animefinge Online Magazine|date=August 2002|website=animefringe|language=en|access-date=July 14, 2020|archive-url=https://web.archive.org/web/20190523143037/https://www.animefringe.com/magazine/02.08/feature/6/index.php3|archive-date=May 23, 2019}}

| Japan

rowspan="4"|2002–2024

|rowspan="4"|Clone High

|rowspan="3"|November 2, 2002

|Cleopatra "Cleo" Smith

|Bisexual

|Cleo is the clone of the Egyptian queen Cleopatra and is the popular girl at Clone High. Cleo was hinted to be bisexual in the episode "Homecoming: A Shot in D'Arc" when she showed romantic interest in Joan of Arc as both herself and her male disguise "John Dark".{{cite web |last1=Cisneros |first1=Ana Iris |title='Clone High:' The Best Episodes from Season 1, According to IMDb |url=https://collider.com/clone-high-best-episodes-season-1-imdb/ |website=Collider |access-date=June 15, 2023 |date=June 15, 2023}} This was confirmed in the season 2 episode "Sexy-Ed" when Cleo confesses her love to Frida Kahlo and the two kiss.{{cite episode|title=Sexy-Ed|series=Clone High|airdate=June 15, 2023|season=2|number=8}}{{better source needed|date=July 2024}}

|rowspan="2"|United States

Wally

|rowspan="2"|Gay

|rowspan="2"|Wally and Carl are JFK's adoptive fathers. They are an interracial couple and JFK refers to them as his "gay foster dads".{{cite web |last1=Roth |first1=Dany |title=Stuff We Love: Christopher Miller and Phil Lord's Clone High |url=https://www.syfy.com/syfywire/stuff-we-love-clone-high |website=Syfy |access-date=May 3, 2020 |archive-url=https://web.archive.org/web/20190516194258/https://www.syfy.com/syfywire/stuff-we-love-clone-high |archive-date=May 16, 2019 |date=February 5, 2017 |url-status=live |df=mdy-all }} The mention of "gay foster dads" refers to Wally and Carl.

Carl

|rowspan="2"|Canada

May 23, 2023

|Frida Kahlo

|Queer

|Frida Kahlo is the clone of the Mexican painter Frida Kahlo. Frida is revealed to be queer in the episode "Sexy-Ed" when she confesses her love to Cleopatra. The historical Frida Kahlo was bisexual.{{cite web |title=What About Frida Kahlo's Bisexuality? |url=https://www.curvemag.com/blog/art/what-about-frida-kahlos-bisexuality/ |website=Curve |access-date=November 9, 2018 |date=June 15, 2023}} Frida Kahlo is voiced by lesbian singer/songwriter and actress Vicci Martinez.

2003

class="wikitable sortable" style="width: 100%"

!scope="col" style="width:10%;"| Duration

!scope="col" style="width:10%;"| Show title

!scope="col" style="width:12%;"| Character debut date

!scope="col" style="width:12%;"| Characters

!scope="col" style="width:12%;"| Identity

! Notes

! Country

rowspan="5"|2003

| Air Master

| April 1, 2003

| Mina Nakanotani

| Lesbian

|Mina is hopelessly in love with Maki after meeting her in the first episode. Although she constantly denies this, she kisses Miki in the show's third episode, and later takes her to a "love hotel."{{cite web |last1=Friedman |first1=Erica |title=Air Master Anime, Volume 3 |url=http://okazu.yuricon.com/2005/05/23/air-master-anime-volume-3/ |website=Okazu |access-date=July 16, 2020 |archive-url=https://archive.today/20200716144214/http://okazu.yuricon.com/2005/05/23/air-master-anime-volume-3/ |archive-date=July 16, 2020 |date=May 23, 2005 |url-status=live |df=mdy-all }} In the manga this anime is based on, Mina also develops a crush on Maki.{{cite web |last1=Friedman |first1=Erica |title=Yuri Manga: Air Master |url=http://okazu.yuricon.com/2004/02/26/yuri-manga-air-master/ |website=Okazu |access-date=July 16, 2020 |archive-url=https://archive.today/20200716145153/http://okazu.yuricon.com/2004/02/26/yuri-manga-air-master/ |archive-date=July 16, 2020 |date=February 26, 2004 |url-status=live |df=mdy-all }}

| Japan

Kino's Journey

| April 8, 2003

| Kino

| Transmasculine

| Kino is a traveler who goes to "unknown mystical places" with a talking motorcycle named Hermes.{{cite web|last=Beard|first=Jeremy A.|url=https://www.themanime.org/viewreview.php?id=752|title=Kino's Journey [Review]|website=THEM Anime Reviews|date=2005|access-date=September 26, 2021|archive-url=https://web.archive.org/web/20210126171513/https://www.themanime.org/viewreview.php?id=752|archive-date=January 26, 2021|url-status=live}}{{cite web|url=http://www.kinosjourney.com/tv.shtml|title=+++ キノの旅 +++|trans-title=+++ Kino's Journey +++ |archive-url=https://web.archive.org/web/20080224033234/http://www.kinonotabi.com/about.html|archive-date=February 24, 2008|url-status=dead|access-date=September 26, 2021|language=ja}} Kino was assigned female at birth, but has a "androgynous persona," alternating between using feminine and masculine pronouns, while resisting those that attempt to pin a gender on them as a "girl" or "boy." While the show's official website, described Kino as a "young man,"{{cite web|url=http://www.kinosjourney.com/tv.shtml|title=Summary|archive-url=https://web.archive.org/web/20031029145909/http://www.kinosjourney.com/tv.shtml|archive-date=October 29, 2003|url-status=dead|access-date=September 26, 2021}} the series creator, Keiichi Sigsawa, said in September 2017, "Kino really doesn't think of herself too deeply when she uses pronouns. Depending on the circumstances, she may use "boku" or "atashi", it kinda depends on the situation she finds herself in," adding that it was not he "wanted to write a story about a girl who lives like a boy directly, [but] it was just an interesting way to start the story, and her character's personality has continued that way since then."{{cite interview |last=Sigsawa |first=Keiichi |subject-link=Keiichi Sigsawa |interviewer=Jacob Chapman |title=Interview: Keiichi Sigsawa |type= |work= |date=September 11, 2017 |publisher=Anime News Network |url=https://www.animenewsnetwork.com/feature/2017-09-11/interview-keiichi-sigsawa/.121076 |url-status=live |archive-url=https://web.archive.org/web/20210422214906/https://www.animenewsnetwork.com/feature/2017-09-11/interview-keiichi-sigsawa/.121076 |archive-date=April 22, 2021 |access-date=September 26, 2021}} As such, some reviewers even described Kino as one of the "rare transmasculine anime protagonists."{{cite web |last1=Baron |first1=Rueben |title=20 Crucial Queer Representations In Anime (For Better Or Worse) |website=CBR |url=https://www.cbr.com/queer-anime-characters/ |access-date=July 20, 2020 |archive-url=https://web.archive.org/web/20190926174900/https://www.cbr.com/queer-anime-characters/ |archive-date=September 26, 2019 |date=June 24, 2018 |url-status=live }}

| Japan

rowspan="3"|Yami to Bōshi to Hon no Tabibito

| rowspan="3"|October 2, 2003

| Hazuki Azuma

| rowspan="2"|Lesbian

| She is a tomboyish female with numerous female admirers, throughout her journeys, who develops a sexual attraction to her adopted older sister, Hatsumi Azuma, who reciprocates.{{cite web |last1=Martin |first1=Theron |title=Yamibo Sub.DVD - Darkness, the Hat, and the Travelers of the Books: Complete Collection [review] |url=https://www.animenewsnetwork.com/review/yamibo/sub.dvd-darkness-the-hat-and-the-travelers-of-the-books/complete-collection/.101888 |website=Anime News Network |access-date=June 30, 2020 |archive-url=https://web.archive.org/web/20190522052021/https://www.animenewsnetwork.com/review/yamibo/sub.dvd-darkness-the-hat-and-the-travelers-of-the-books/complete-collection/.101888 |archive-date=May 22, 2019 |date=June 5, 2016 |url-status=live |df=mdy-all }}{{cite web|last=Foote|first=Alan|url=https://www.themanime.org/viewreview.php?id=750|title=Yami to Boushi to Hon no Tabibito [Review]|website=THEM Anime Reviews|date=2011|access-date=August 28, 2021|archive-url=https://web.archive.org/web/20111222012034/https://www.themanime.org/viewreview.php?id=1295|archive-date=December 22, 2011|url-status=live}} After Hatsumi disappears on her 16th birthday, Hazuki goes world-hopping in hopes of finding Hazuki,{{cite web|url=http://www.orbit-soft.com/product/root/yami/yami_story.htm|title=ヤミと帽子と本の旅人|trans-title=Yami, Hat, and Book Traveler|website=Orbit Soft|publisher=|access-date=October 4, 2021|archive-url=https://web.archive.org/web/20051028160240/http://www.orbit-soft.com/product/root/yami/yami_story.htm|archive-date=October 28, 2005|url-status=dead}} which her companions, a bird, and a woman named Lilith, known by the name of "Eve." Her love is deep-rooted, seemingly reciprocated through love letters from Hatsumi while she lived in Japan, and encounters a woman named Fujihime in a feudal Japanese world whom looks almost identical to Hatsumi.{{cite web|url=http://www.orbit-soft.com/product/root/yami/chara/fujihime.htm|title=ヤミと帽子と本の旅人キャラクター紹介『藤姫』|trans-title=Yami, Hat, and Book Traveler Character Introduction "Fujihime"|website=Orbit Soft|access-date=October 4, 2021|archive-url=https://web.archive.org/web/20060220171003/http://www.orbit-soft.com/product/root/yami/chara/fujihime.htm|archive-date=February 20, 2006|url-status=dead}} In the final episode, "Lilith," they kiss each other, fulfilling Hazumi's wish.{{cite web|url=http://www.orbit-soft.com/product/root/yami/chara/fujihime.htm|title=ヤミと帽子と本の旅人キャラクター紹介『制服の少女』|trans-title=Yami, Hat, and Book Traveler Character Introduction "Girl in Uniform" |website=Orbit Soft|access-date=October 4, 2021|archive-url=https://web.archive.org/web/20051206033149/http://www.orbit-soft.com/product/root/yami/chara/shoujo.htm|archive-date=December 6, 2005|url-status=dead}}

| rowspan="3"|Japan

Hatsumi Azuma

| The adopted older sister of Hazuki Azuma, she develops an attraction to her,{{cite web|url=http://www.animax.co.jp/feature/index.php?program=NN10000380|title=ANIMAX アニメ見るならアニマックス：番組表 - ヤミと帽子と本の旅人|trans-title=ANIMAX If you want to watch anime Animax: Program guide-Yami to Hat and Book Traveler|archive-url=https://web.archive.org/web/20070308061523/http://www.animax.co.jp/feature/index.php?program=NN10000380|archive-date=March 8, 2007|access-date=October 4, 2021|url-status=dead}} and kisses her in the show's final episode.{{cite web|last=Tiu|first=Diane|url=https://www.themanime.org/viewreview.php?id=750|title=Yami to Boushi to Hon no Tabibito [Review]|website=THEM Anime Reviews|date=2003|access-date=August 28, 2021|archive-url=https://archive.today/20130113071903/http://www.themanime.org/viewreview.php?id=750|archive-date=January 13, 2013|url-status=live}}

Lilith

| Possibly bisexual

| Lilith, the guardian of the Great Library, who has taken on the title "Yami,"{{cite web|url=http://www.orbit-soft.com/product/root/yami/chara/yami.htm|title=ヤミと帽子と本の旅人キャラクター紹介『ヤミ』|trans-title=Yami, hat, and book traveler character introduction "Yami"|website=Orbit Soft|access-date=October 4, 2021|archive-url=https://web.archive.org/web/20051028160034/http://www.orbit-soft.com/product/root/yami/chara/yami.htm|archive-date=October 28, 2005|url-status=dead}} flirts relentlessly with Hazuki. Sometimes she goes to absurd lengths to show her love in various book worlds, sometimes even trying to sleep with her, as shown in episodes such as "Quill" and "Layla." Lilith's previous lover was the library's previous protector, Adam, referred to as a male character in the third episode. While Lilith is close to her sister, Hitsumi, whose real name is Eve, she only loves her in a platonic way.

2003–2004

| Fullmetal Alchemist

| October 11, 2003

| Envy

| Genderqueer

| Envy is a shapeshifting homunculus without a specific gender.{{cite web |last1=Loveridge |first1=Lynzee |title=8 Creepy Creatures Worth Screaming About |url=https://www.animenewsnetwork.com/the-list/2012-10-06 |website=Anime News Network |access-date=July 16, 2020 |archive-url=https://web.archive.org/web/20200112042223/https://www.animenewsnetwork.com/the-list/2012-10-06 |archive-date=January 12, 2020 |date=October 6, 2012 |url-status=live |df=mdy-all }}{{cite press release |date=November 1, 2018 |title=Upcoming Fullmetal Alchemist Brotherhood and The Alchemist Code Collaboration this November |url=https://www.animenewsnetwork.com/press-release/2018-11-01/upcoming-fullmetal-alchemist-brotherhood-and-the-alchemist-code-collaboration-this-november/.138893 |location= |publisher=Anime News Network |agency=gumi Inc. |access-date=September 27, 2021 |archive-url=https://web.archive.org/web/20181101103044/https://www.animenewsnetwork.com/press-release/2018-11-01/upcoming-fullmetal-alchemist-brotherhood-and-the-alchemist-code-collaboration-this-november/.138893 |archive-date=November 1, 2018 |url-status=live |df=mdy-all}} While they are usually referred to in general neutral pronouns in the Japanese version, in both anime they are usually referred to as he, indicate in episodes like "Death." They are normally seen to have the appearance of an 'androgynous' female.

| Japan

rowspan="2"|2003-2020

| rowspan="2"|Red vs. Blue

| October 2, 2016

| Agent Ohio "Vera"

| rowspan="2"|Lesbian

| Agent Ohio is a lesbian woman and an agent of Project Freelancer who appeared in two episodes of Season 14, specifically "The Triplets" and "The "Mission"". Her sexuality was confirmed by the episode's writer, Shannon McCormick.{{cite tweet |last=McCormick |first=Shannon |author-link=Shannon McCormick |user=sadogre |number=782679772572753920 |date=October 2, 2016 |title=in my mind, lesbian. |website=Twitter |access-date=March 7, 2020 |archive-url=https://web.archive.org/web/20200515013706/https://twitter.com/sadogre/status/782679772572753920 |archive-date=May 15, 2020 |url-status=live}}

| rowspan="2"|United States

October 9, 2016

| Sherry

| Sherry is a lesbian woman and a soldier for Charon Industries who appeared in one episode of Season 14, "The Mission." Her sexuality was confirmed by the episode's writer, Shannon McCormick in the same string of tweets where he said Vera was lesbian.

rowspan="3"|2003-2022

| rowspan="3"|Ikki Tousen

| August 6, 2003

| Koudai Chinkyuu

| Lesbian

| rowspan="2"| Chinkyuu is Ryofu's childhood friend and lesbian lover, and a C-rank fighter with great potential.{{cite web|title=Chinkyū Kōdai |url=https://lezwatchtv.com/character/chinkyu-kodai/ |access-date=August 20, 2024 |website=Lezwatch.tv }}{{cite web|title=Housen Ryofu |url=https://lezwatchtv.com/character/housen-ryofu/ |access-date=August 20, 2024 |website=Lezwatch.tv }}

| rowspan="3"|Japan

August 13, 2003

| Housen Ryofu

| Bisexual

February 26, 2007

| Uncho Kan'u

| Lesbian

| Kan'u is a third-year student at Seito Academy and an A-ranked fighter, who's in love with Ryubi.{{cite web|title=Kan'u Unchō |url=https://lezwatchtv.com/character/kanu-uncho/ |access-date=August 20, 2024 |website=Lezwatch.tv }}

rowspan="2"|2003–present

| rowspan="2"|Eddsworld

| rowspan="2"|June 6, 2003

| Kim

| Bisexual

| Todd, one of the show's protagonists, thinks Kim is interested in him, but she isn't, and only is messing and flirting with him.{{Cite episode |title=The Dudette Next Door |url=https://www.youtube.com/watch?v=EPgm_Oj9u1g |access-date=September 13, 2021 |series=Eddsworld |network=YouTube |date=June 21, 2007 |season=1 |number=10 |language=en}} Instead she kisses her girlfriend, Katya, to his chagrin, in the episode "The Dudette Next Door."

| rowspan="2"|United Kingdom

Katya

| Lesbian

| She has a girlfriend, Kim, who she loves very much, kissing her intensely in one episode. Like Kim, she is voiced by Kira Buckland.

2004

class="wikitable sortable" style="width: 100%"

!scope="col" style="width:10%;"| Duration

!scope="col" style="width:10%;"| Show title

!scope="col" style="width:12%;"| Character debut date

!scope="col" style="width:12%;"| Characters

!scope="col" style="width:12%;"| Identity

! Notes

! Country

rowspan="8"|2004

|Burst Angel

|April 6, 2004

|Megumi "Meg"

| Lesbian

|She is the very best of friends with Jo and is 100% loyal only to her. Since Meg was once an orphan in New York City, Meg and Jo partnered and became bounty hunters together.{{cite web |last1=Martin |first1=Theron |title=Burst Angel Blu-ray [Review] |url=https://www.animenewsnetwork.com/review/burst-angel/blu-ray/.146429 |website=Anime News Network |access-date=July 16, 2020 |archive-url=https://web.archive.org/web/20190703132936/https://www.animenewsnetwork.com/review/burst-angel/blu-ray/.146429 |archive-date=July 3, 2019 |date=May 8, 2019 |url-status=live |df=mdy-all }} While the anime tries to keep her relationship with Jo on the verge of friendly affection and still maintain a few subtle subtext, the three-volume manga prequel openly shows that Meg is experiencing a sexual attraction to her friend,{{cite web |last1=Friedman |first1=Erica |title=Yuri Manga: Burst Angel, Volume 1 (English) |url=http://okazu.yuricon.com/2008/10/01/yuri-manga-burst-angel-volume-1-english/ |website=Okazu |access-date=July 16, 2020 |archive-url=https://web.archive.org/web/20180320043710/http://okazu.yuricon.com/2008/10/01/yuri-manga-burst-angel-volume-1-english/ |archive-date=March 20, 2018 |date=October 1, 2008 |url-status=live |df=mdy-all }}{{cite web |last1=Friedman |first1=Erica |title=Yuri Manga: Burst Angel, Vol[u]me 2 (English) |url=http://okazu.yuricon.com/2009/03/18/yuri-manga-burst-angel-volme-2-english/ |website=Okazu |access-date=July 16, 2020 |archive-url=https://web.archive.org/web/20150926030626/http://okazu.yuricon.com/2009/03/18/yuri-manga-burst-angel-volme-2-english/ |archive-date=September 26, 2015 |date=March 18, 2009 |url-status=live |df=mdy-all }} fantasizing about their sex or even openly trying to seduce Jo.{{cite web |last1=Friedman |first1=Erica |title=Yuri Manga: Burst Angel, Volume 3 (English) |url=http://okazu.yuricon.com/2009/06/19/yuri-manga-burst-angel-volume-3-english/ |website=Okazu |access-date=July 16, 2020 |archive-url=https://web.archive.org/web/20170706100113/http://okazu.yuricon.com/2009/06/19/yuri-manga-burst-angel-volume-3-english/ |archive-date=July 6, 2017 |date=June 19, 2009 |url-status=live |df=mdy-all }} This is later referenced in the OVA sequel, "Jo and Meg Blues."{{cite web |title=Burst Angel OVA 25: The Lightness and Darkness of Jo |url=https://www.funimation.com/shows/burst-angel/the-lightness-and-darkness-of-jo/?qid=undefined |website=Funimation |access-date=October 8, 2021 |archive-url=https://archive.today/20200717020431/https://www.funimation.com/shows/burst-angel/the-lightness-and-darkness-of-jo/ |archive-date=July 17, 2020 |date=2007 |url-status=dead |df=mdy-all }} Funimation titles this OVA "The Lightness and Darkness of Jo."

| Japan

rowspan="2"|Doki Doki School Hours

|rowspan="2"|April 4, 2004

|Rio Kitagawa

| Lesbian

| She is a lesbian and has stated on several occasions that she loves small girls, thus making it no surprise that she has a huge (and slightly sadistic) crush on Mika-sensei.{{cite web |last1=Jones |first1=Tim |title=Doki Doki School Hours [Review] |url=https://www.themanime.org/viewreview.php?id=1034 |website=T.H.E.M. Anime Reviews |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20190725203918/http://themanime.org/viewreview.php?id=1034 |archive-date=July 25, 2019 |date=2008 |url-status=live |df=mdy-all }} She is constantly flirting with her, which often borders on molestation.{{cite web|last=Santos|first=Carlo|title=Doki Doki School Hours DVD 1: 1st Hour [Review]|url=https://www.animenewsnetwork.com/review/doki-doki-school-hours/dvd-1|website=Anime News Network|date=|access-date=August 28, 2021|archive-url=https://web.archive.org/web/20210730011536/https://www.animenewsnetwork.com/review/doki-doki-school-hours/dvd-1|archive-date=July 30, 2021|url-status=live}} She also does well academically, though she may fail the test on purpose if it means she will get to spend time with Mika-sensei.

| rowspan="2"|Japan

Yuichi Kudo

| Gay

| A male honor student who is gay and in love with Suetake, clear from the show's first episode.{{cite web |last1=Santos |first1=Carlo |title=Doki Doki School Hours DVD 2: 2nd Hour [Review] |url=https://www.animenewsnetwork.com/review/doki-doki-school-hours/dvd-2 |website=Anime News Network |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20190725030423/https://www.animenewsnetwork.com/review/doki-doki-school-hours/dvd-2 |archive-date=July 25, 2019 |date=February 13, 2008 |url-status=live |df=mdy-all }} Though Suetake is completely oblivious to Kudo's affections, everyone else is aware of them, and they are constantly rolling their eyes over his actions (which is very similar to how they react to Kitagawa's love for Mika, the only difference being that Mika is aware of Kitagawa's feelings, but can do little to stop her advances). He is seen having fantasies about Suetake, and not much else.

rowspan="3"|Kannazuki no Miko

| rowspan="3"|October 1, 2004

| Himeko Kurusugawa

| Bisexual

| At first, Himeko is romantically interested in her childhood friend Sōma Ōgami.{{cite web|url=http://www.kadokawa.co.jp/sp/200409-10/cts002.html|title=++ 神無月の巫女 ++|trans-title=++ Kannazuki no Miko ++ |website="Kannazuki no Miko" Official Homepage|publisher=Kadokawa Shoten|archive-url=https://web.archive.org/web/20050211074344/http://www.kadokawa.co.jp/sp/200409-10/cts002.html|archive-date=February 11, 2005|url-status=live}}{{cite web|url=http://www.kannaduki.info/epi4.html|title=第四話『思い賜うや』|trans-title=Episode 4 "Thinking" |website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20181107063020/http://www.kannaduki.info/epi4.html|archive-date=November 7, 2018|url-status=live|language=ja}}{{cite web|url=http://www.kannaduki.info/character.html|title=神無月の巫女|trans-title=Kannazuki no Miko|website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20210603052257/http://www.kannaduki.info/character.html|archive-date=June 3, 2021|url-status=live|language=ja}} During the last few episodes of the series, Himeko realizes and accepts her feelings for Chikane, with Himeko reunited with Chikane. In the show's final episode, they kiss after admitting their feelings for each other and are reunited even after they are reincarnated after the defeat of the Orochi.{{cite web|url=http://www.kannaduki.info/epi12.html|title=最終話『神無月の巫女』|trans-title=The final episode "Kannazuki no Miko" |website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20150410072600/http://www.kannaduki.info/epi12.html|archive-date=April 10, 2015|url-status=live|language=ja}}

| rowspan="3"|Japan

Chikane Himemiya

| rowspan="2"|Lesbian

| Chikane has loved Himeko since they met,{{cite web|url=http://www.kannaduki.info/epi6.html|title=第六話『日溜まりの君』|trans-title=Episode 6 "Kimi of the Sun" |website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20091022041030/http://www.kannaduki.info/epi6.html|archive-date=October 22, 2009|url-status=live|language=ja}} although (despite her jealousy of Himeko and Sōma's friendship){{cite web|url=http://www.kannaduki.info/epi5.html|title=第五話『夜闇を越えて』|trans-title=Episode 5 "Beyond the Darkness" |website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20090924094534/http://www.kannaduki.info/epi5.html|archive-date=September 24, 2009|url-status=live|language=ja}} she does not admit it until much later, with Chikane's feelings for Himeko bordering on obsession.{{cite web |last1=Jones |first1=Tim |title=Destiny of the Shrine Maiden [Review] |url=https://www.themanime.org/viewreview.php?id=826 |website=THEM Anime Reviews |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20190730142210/http://themanime.org/viewreview.php?id=826 |archive-date=July 30, 2019 |date=2006 |url-status=live |df=mdy-all }} She eventually turns evil upon joining the Orochi as the Eighth Head, vanquishing all but two of the Orochi heads, sexually assaulting Himeko in the 8th episode,{{cite web|url=https://www.viz.com/kannazuki-no-miko|title=The Official Website for Kannazuki no Miko|publisher=Viz Media|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20210228131110/https://www.viz.com/kannazuki-no-miko|archive-date=February 28, 2021|url-status=live|quote=Pretty and popular Chikane has two secrets...she's in love with shy Himeko...Chikane...assaults Himeko to make her friend unfit as a priestess.}}{{cite web|url=http://www.kannaduki.info/epi8.html|title=第八話『銀月の嵐』|trans-title=Episode 8 "Silver Moon Storm" |website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20091010202916/http://www.kannaduki.info/epi8.html|archive-date=October 10, 2009|url-status=live|language=ja}}{{cite web|url=http://www.kannaduki.info/epi9.html|title=第九話『黄泉比良坂へ』|trans-title=Episode 9 "To Yomotsuhirasaka" |website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20100404070325/http://www.kannaduki.info/epi9.html|archive-date=April 4, 2010|url-status=live|language=ja}} and tormenting her during their final battle.{{cite web|url=http://www.kannaduki.info/epi11.html|title=第十一話『剣の舞踏会』|trans-title=Episode 11 "Sword Ball" |website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20100404064056/http://www.kannaduki.info/epi11.html|archive-date=April 4, 2010|url-status=live|language=ja}}

Otoha Kisaragi

| Devoted maid to Chikane, makes clear that Chikane reserved her feelings only for Himeko and is, as such, jealous of Himeko. When Otoha is sent away, in the episode "Rainfall in the Hell of Love," she expresses her love and admiration for Chikane.{{cite web|url=http://www.kannaduki.info/epi10.html|title=第十話『愛と死の招待状』|trans-title=Episode 10 "Love and Death Invitation"|website=Kannazuki no Miko|publisher=Kaishaku|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20100403015553/http://www.kannaduki.info/epi10.html|archive-date=April 3, 2010|url-status=live|language=ja}}

rowspan="2"|Re: Cutie Honey

| rowspan="2"|July 24, 2004

| Cutie Honey

| rowspan="2"|Lesbian

| rowspan="2"|Inspector Natsuko Aki grows close to Cutie Honey, otherwise known as Honey Kisaragi, apologizing for calling her a "nuisance" and bringing her back to life by lying naked with her, allowing their hearts to connect, returning her energy in the episode "Earth's Volume."{{cite web |last1=Friedman |first1=Erica |title=Yuri Anime: Re Cutie Honey |url=https://okazu.yuricon.com/2004/11/05/yuri-anime-re-cutie-honey/ |website=Okazu |access-date=December 7, 2020 |archive-url=https://web.archive.org/web/20201018220741/https://okazu.yuricon.com/2004/11/05/yuri-anime-re-cutie-honey/ |archive-date=October 18, 2020 |date=November 5, 2004 |url-status=live |df=mdy-all }} In the final episode, "Humanity's Volume," Natsuko cares for Cutie after a fight with a guardian of the Panther Claw. After she gives Cutie a pep talk, raising her spirits, Cutie embraces her, saying she "loves" her, and they have a tender moment before beginning the major battle.{{cite web |author= |title=Characters |url=http://www.gainax.co.jp/anime/re_cutie/story.html |website=Re: Cutie Honey official website |publisher=LATERNA, Toei Video, Gainax, Towani |access-date=June 30, 2020 |archive-url=https://web.archive.org/web/20050206214238/http://www.gainax.co.jp/anime/re_cutie/story.html |archive-date=February 6, 2005 |date=February 6, 2005 |url-status=live |df=mdy-all }} At the close of the episode, Cutie and Natsuko work together in a detective agency, fighting evildoers.

| rowspan="2"|Japan

Natsuko Aki

rowspan="3"|2004–2005

| Gankutsuou: The Count of Monte Cristo

| October 5, 2004

| Franz d'Épinay

| Bisexual

| Franz, a friend of Albert and "strong subordinate",{{cite web|url=http://www.gankutsuou.com/world/original.html|title=The Original Story|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20080421031649fw_/http://www.gankutsuou.com/world/original.html|website=Gankutsuou|publisher=Mahiro Media|archive-date=April 21, 2008|url-status=live|language=ja}} is engaged to a woman named Valentine for political reasons though he does not love her. He instead heavily implies throughout the series that he has strong feelings for someone else and it is later revealed his feelings are towards his best friend Albert,{{cite web|url=http://www.gankutsuou.com/world/character/character03.html|title=Characters--Franz d'Épinay|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20080423204501fw_/http://www.gankutsuou.com/world/character/character03.html|archive-date=April 23, 2008|website=Gankutsuou|publisher=Mahiro Media|url-status=live|language=ja}}{{cite web |last1=Douglass, Jr. |first1=Todd |title=Gankutsuou: Count of Monte Cristo - The Complete Series |url=https://www.dvdtalk.com/reviews/37180/gankutsuou-count-of-monte-cristo-the-complete-series/ |website=DVD Talk |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20151027200429/http://www.dvdtalk.com/reviews/37180/gankutsuou-count-of-monte-cristo-the-complete-series/ |archive-date=October 27, 2015 |date=April 28, 2009 |url-status=live |df=mdy-all }} who is traveling with him.{{cite web |last1=Høgset |first1=Stig |title=Gankutsuou: The Count of Monte Cristo [Review] |url=https://www.themanime.org/viewreview.php?id=864 |website=THEM Anime Reviews |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20190307133901/https://www.themanime.org/viewreview.php?id=864 |archive-date=March 7, 2019 |date=2006 |url-status=live |df=mdy-all }} However, he did show some attraction to women and did consider sleeping with a woman in episode 1, "At Journey's End, We Meet."

| Japan

rowspan="2"|My-HiME

| rowspan="2"|September 30, 2004

| Natsuki Kuga

| rowspan="2"|Lesbian

| In the carnival, it was revealed that Shizuru is Natsuki's most important person but because of Shizuru's psychotic rampage Natsuki began to doubt her feelings, as before that time, their love was only implied.{{cite web |last1=Friedman |first1=Erica |title=Yuri Anime: My Zhime (Mai Otome), Volume 1 (English) |url=http://okazu.yuricon.com/2007/08/31/yuri-anime-my-zhime-mai-otome-volume-1-english/ |website=Okazu |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20150930201118/http://okazu.yuricon.com/2007/08/31/yuri-anime-my-zhime-mai-otome-volume-1-english/ |archive-date=September 30, 2015 |date=August 31, 2007 |url-status=live |df=mdy-all }}{{cite web |last1=Friedman |first1=Erica |title=Yuri Anime: Mai Otome |url=http://okazu.yuricon.com/2006/04/07/yuri-anime-mai-otome/ |website=Okazu |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20160727145305/http://okazu.yuricon.com/2006/04/07/yuri-anime-mai-otome/ |archive-date=July 27, 2016 |date=April 7, 2006 |url-status=live |df=mdy-all }} Natsuki also loves Shizuru back.{{cite web|url=https://www.sunrise-inc.co.jp/my-hime/web/pv/15.html|title=第15話『天翔けるﾐ☆ 女子高生』|trans-title=Episode 15 "A High School Girl Soaring" |website=[舞-HiME] 風華学園オフィシャルWeb - STORY|publisher=Sunrise|date=January 13, 2004|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20080327200647/http://www.sunrise-inc.co.jp/my-hime/web/pv/15.html|archive-date=March 27, 2008|url-status=live}} Kuga's voice actress Saeko Chiba said that by the end of the series she had "opened her heart more and more...[and] accepted people properly without knowing it."{{cite web|url=https://www.sunrise-inc.co.jp/my-hime/web/character/index.html|title=Character|website=Mai-HiME|publisher=Sunrise|date=2004|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20210502153541/https://www.sunrise-inc.co.jp/my-hime/web/character/index.html|archive-date=May 2, 2021|url-status=live}}

| rowspan="2"|Japan

Shizuru Fujino

| She harbors a secret obsessive infatuation with Natsuki Kuga and they remove themselves from the tournament by killing each other, but are resurrected in the final episode.{{cite web|url=https://www.sunrise-inc.co.jp/my-hime/web/pv/26pre.html|title=第26話『shining☆days』>> 予告編を見る|trans-title=Episode 26 "shining ☆ days" >> Watch the trailer |website=[舞-HiME] 風華学園オフィシャルWeb - STORY|publisher=Sunrise|date=March 31, 2005|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20071219123944/http://www.sunrise-inc.co.jp/my-hime/web/pv/26pre.html|archive-date=December 19, 2007|url-status=live}}{{cite web|url=https://www.sunrise-inc.co.jp/my-hime/web/pv/26.html|title=第26話『shining☆days』|trans-title=Episode 26 "shining ☆ days" |website=[舞-HiME] 風華学園オフィシャルWeb - STORY|publisher=Sunrise|date=March 31, 2005|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20071219120514/http://www.sunrise-inc.co.jp/my-hime/web/pv/26.html|archive-date=December 19, 2007|url-status=live}} Natsuki accepts Shizuru's feelings after giving Takeda a letter explaining her rejection.{{cite web |last1=Friedman |first1=Erica |title=Mai Otome Zwei OAV Anime |url=http://okazu.yuricon.com/2007/01/10/mai-otome-zwei-oav-anime/ |website=Okazu |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20170706140449/http://okazu.yuricon.com/2007/01/10/mai-otome-zwei-oav-anime/ |archive-date=July 6, 2017 |date=January 10, 2007 |url-status=live |df=mdy-all }}{{cite web|last=Martin|first=Theron|url=https://www.animenewsnetwork.com/review/my-hime/dvd-7|title=My-HiME DVD 7 - Special Edition + Artbox [Review]|website=Anime News Network|date=May 11, 2007|archive-url=https://web.archive.org/web/20181112030533/https://www.animenewsnetwork.com/review/my-hime/dvd-7|archive-date=November 12, 2018|url-status=live|access-date=August 28, 2021}} The voice actress for Fujino, Naomi Shindo wondered if the audience could sympathize with the character, calling her an "unpleasant woman," and wondered how the feelings in the character's heart would change people.{{cite web|url=https://www.sunrise-inc.co.jp/my-hime/web/character/chara/shizuru.html|title=[舞-HiME] 風華学園オフィシャルWeb - 本校在籍者|trans-title=[Mai-HiME] Fuka Gakuen Official Web-Our school enrolled |website=[舞-HiME] 風華学園オフィシャルWeb - STORY|publisher=Sunrise|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20210417113222/https://www.sunrise-inc.co.jp/my-hime/web/character/chara/shizuru.html|archive-date=April 17, 2021|url-status=live}}

rowspan="4"|2004–2006

| Kyo Kara Maoh!

| April 3, 2004

|Wolfram von Bielefeld

| Gay

| In another dimension called Shin Makoku, love between the same gender is not rare at all. Wolfram became Shibuya Yuuri's accidental fiancé after Yuuri slaps him on his left cheek for insulting his mother which actually is a traditional proposal amongst the nobles in Shin Makoku.{{cite web|url=http://www9.nhk.or.jp/anime/mao/ch_wolfram.html|title=NHKアニメワールド：今日からマ王！|trans-title=NHK Anime World: Maou from today!|website=Kyo Kara Maoh! official website|publisher=NHK|archive-url=https://web.archive.org/web/20120704044721fw_/http://www9.nhk.or.jp/anime/mao/ch_wolfram.html|archive-date=July 4, 2012|url-status=live}}{{cite web |last1=Santos |first1=Carlo |title=Kyo Kara Maoh! DVD 1 [Review] |url=https://www.animenewsnetwork.com/review/kyo-kara-maoh/dvd-1 |website=Anime News Network |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20190315201442/https://www.animenewsnetwork.com/review/kyo-kara-maoh/dvd-1 |archive-date=March 15, 2019 |date=March 15, 2019 |url-status=live |df=mdy-all }}

| Japan

rowspan="3"|W.I.T.C.H.

| December 18, 2004

| Irma

| rowspan="2"|Possibly lesbian

| In April 2016, Greg Weisman, the producer of Season 2, said that she is a lesbian, only interested in "guys that are obviously out of reach," trying to be straight, but by doing this, she is "guaranteeing that she won't have to actually wind up with a guy" and when she comes close, she "comes close to landing one of them, she bolts."{{Cite web|last=Weisman|first=Greg|url=https://www.s8.org/gargoyles/askgreg/search.php?qid=20761|title=EXALT writes...|website=A Station Eight Fan Web Site |date=April 18, 2016|language=en|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20201026151518/https://www.s8.org/gargoyles/askgreg/search.php?qid=20761|archive-date=October 26, 2020|url-status=live}} Even so, he left it open to interpretation, saying that this doesn't make it "impossible for her to be straight or bisexual or whatever."

| rowspan="3"|United States

February 12, 2005

| Nerissa

| She was a lover of Cassidy, who killed Cassidy when both were in a romantic relationship with one another, according to Weisman. Both also began a relationship, in Weisman's words, after spending a lot of time together as teammates, and said that she "loved and mourned Cassidy."{{Cite web|last=Weisman|first=Greg|url=https://www.s8.org/gargoyles/askgreg/search.php?qid=20761|title=Hazecrisp writes...|website=A Station Eight Fan Web Site |date=January 20, 2017|language=en|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20201026151518/https://www.s8.org/gargoyles/askgreg/search.php?qid=20761|archive-date=October 26, 2020|url-status=live}}{{Cite web|last=Weisman|first=Greg|url=https://www.s8.org/gargoyles/askgreg/search.php?qid=18329|title=Akino writes...|website=A Station Eight Fan Web Site |date=April 18, 2013|language=en|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20170828053454/http://www.s8.org/gargoyles/askgreg/search.php?qid=18329|archive-date=August 28, 2017|url-status=live}} Even so, Weisman did not correct a fan when they described Nerissa as bisexual.

August 21, 2006

| Cassidy

| Lesbian

| Weisman described Cassidy as a lesbian, said that Cassidy and Nerissa were lovers, and told a fan that they were in a relationship when Nerissa killed Cassidy.{{Cite web|last=Weisman|first=Greg|url=https://www.s8.org/gargoyles/askgreg/search.php?qid=20652|title=Anonymous writes...|website=A Station Eight Fan Web Site|date=February 12, 2016|language=en|access-date=October 8, 2021|archive-url=https://web.archive.org/web/20160805015123/https://www.s8.org/gargoyles/askgreg/search.php?qid=20652|archive-date=August 5, 2016|url-status=live}}

rowspan="4"|2004–2007

| rowspan="4"|Drawn Together

| rowspan="4" | October 27, 2004

| Ling-Ling

| rowspan="2"|Bisexual

|Ling-Ling, an Asian trading-card mini-monster,{{cite web |last1=Oldenburg |first1=Ann |title='Drawn': Animated raunch meets reality TV |url=https://usatoday30.usatoday.com/life/television/news/2004-10-26-drawn-together-main_x.htm |website=USA Today |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20160420091550/http://usatoday30.usatoday.com/life/television/news/2004-10-26-drawn-together-main_x.htm |archive-date=April 20, 2016 |date=October 27, 2004 |url-status=live |df=mdy-all }}{{cite web |last1=Heffernan |first1=Virginia |title=Cartoon Goal: Parody of Self-Parody |url=https://www.nytimes.com/2004/10/27/arts/television/cartoon-goal-parody-of-selfparody.html |website=New York Times |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20171030094211/http://www.nytimes.com/2004/10/27/arts/television/cartoon-goal-parody-of-selfparody.html |archive-date=October 30, 2017 |date=October 27, 2004 |url-status=live |df=mdy-all }} is identified as bisexual by a parody of The Terminator in the episode "Wooldoor Sockbat's Giggle-Wiggle Funny Tickle Non-Traditional Progressive Multicultural Roundtable!"{{cite journal |last1=Misits |first1=Éva |date=2006 |title='Just when you thought racism couldn't get any more racismer': The Treatment of Stereotypes in Drawn Together |url=https://americanaejournal.hu/vol2no2/misits |journal=Americana: e-Journal of American Studies in Hungary |volume=2 |issue=2 |access-date=July 17, 2020}}

| rowspan="4"|United States

Foxxy Love

| Foxxy, a "sexy mystery-solver," has relations with both men and women, but preferably with the former. She makes out with Princess Clara in the show's first episode, and has a brief BDSM relationship with Captain Hero in another episode, "Requiem for a Reality Show."

Xandir P. Whifflebottom

| Gay

| Xandir is a homosexual and effeminate video game platformer. He is thought to be straight when on a "never-ending quest to save his girlfriend" but is revealed to be gay, as first shown in the episode "Gay Bash" when the Wood Beast, a creature which bites off the arms of homosexuals, bites off his arm. He is also labeled as a "gay video game adventurer" in the first episode. He is a parody of the video game heroes such as Link from The Legend of Zelda series and in another episode, "Xandir and Tim, Sitting in a Tree," Xandir has an affair with Captain Hero's gay alter ego, Tim Tommerson. Xandir is voiced by gay actor Jack Plotnick.

Captain Leslie Hero

| Pansexual

| Captain Hero, a lecherous parody of Superman and other superheroes, is a "brawny classic cartoon superhero" who is shown to be sexually aroused, due to his erectile dysfunction, by an array of paraphilias like necrophilia and will have sex with anything and anyone. He and Foxxy Love form a brief BDSM relationship in one episode, "Requiem for a Reality Show," and as his gay alter ego Tim Tommerson, has an affair with Xandir in another episode, "Xandir and Tim, Sitting in a Tree."

rowspan="3"|2004–2009

| bro'Town

| September 22, 2004

| Brother Ken

| Fa'afafine

| Brother Ken is the principal of the school and is fa'afafine,{{cite web |url=https://teara.govt.nz/en/artwork/28877/brother-ken-brotown |title=Brother Ken, bro'Town (1st of 3) |last=Schmidt |first=Johanna |year=2021 |orig-year=2011 |website=Te Ara: The Encyclopedia of New Zealand |publisher=New Zealand Ministry for Culture and Heritage |language=en |access-date=October 8, 2021 |url-status=live |archive-url=https://web.archive.org/web/20190208065054/https://teara.govt.nz/en/artwork/28877/brother-ken-brotown |archive-date=February 8, 2019 }} a person in Samoa, American Samoa and the Samoan diaspora who identifies themselves as having a third gender or non-binary role, and is an integral part of Samoan culture. Those who are fa'afafine have behaviour which ranges from extravagantly feminine to conventionally masculine.{{Cite journal | doi = 10.1007/s10508-006-9055-1| pmid = 16909317| title = A Retrospective Study of Childhood Gender-Atypical Behavior in Samoan Faʻafafine| journal = Archives of Sexual Behavior| volume = 35| issue = 6| pages = 659–66| year = 2006| last1 = Bartlett | first1 = N. H. | last2 = Vasey | first2 = P. L. | s2cid = 22812712}} Because the concept does not readily translate, when the series was broadcast on Adult Swim Latin America, a decision was made not to translate Samoan words and just present them as part of the "cultural journey".{{cite book|last1=Johnson|first1=Derek|last2=Kompare|first2=Derek|last3=Santo|first3=Avi|title=Making Media Work: Cultures of Management in the Entertainment Industries|url=https://books.google.com/books?id=fhToAwAAQBAJ&pg=PT57|access-date=January 1, 2015|date=August 1, 2014|publisher=NYU Press|isbn=9780814764558|pages=57–59}}

| New Zealand

rowspan="2"|Maria-sama ga Miteru

| January 21, 2004

| Suguru Kashiwagi

| Gay

| In the anime, Kashiwagi, who is student council president of an all-boys school named Hanadera Academy, is said to be engaged to Sachiko Ogasawara,{{cite web|url=http://www.gokigenyou.com/2nd/chara05.htm|title=その他|trans-title=Others|publisher=Oyuki Konno/Shueisha|website=Lillian Jogakuen|archive-url=https://web.archive.org/web/20060719035616/http://www.gokigenyou.com/2nd/chara05.htm|archive-date=July 19, 2006|url-status=dead|quote=He is a third-year student at Hanadera Gakuin High School and a student council president. He played the role of a prince in the Yamayurikai play "Cinderella" at the school festival. He is also a cousin of Shoko and a fiancé.}} but is not interested in her, drawing her anger. The official site for the series said that Kashiwagi nicknames Yuki Fukuzawa "Yukichi" and says he is "strangely fond of him."{{cite web|url=http://www.tv-tokyo.co.jp/anime/mariasama_haru/character/yu_ki.html|title=ふくざわ　ゆうき　花寺学院２年生|trans-title=Fukuzawa Yuki, 2nd year student at Hanadera Academy|website=Konno Oyuki/Shueisha, Yamayuri Association|publisher=TV Tokyo|archive-url=https://web.archive.org/web/20121028103240fw_/http://www.tv-tokyo.co.jp/anime/mariasama_haru/character/yu_ki.html|archive-date=October 28, 2012}} In the manga and novels it is confirmed that Kashiwagi is gay because he is interested in Yuki, the brother of Yumi, who is the sœur of Sachiko.{{cite web|last=Friedman|first=Erica|url=https://okazu.yuricon.com/2004/02/06/maria-sama-ga-miteru-thoughts-on-the-first-story-arc/|title=Maria-sama ga Miteru: Thoughts on the first story arc|website=Okazu|date=February 6, 2004|access-date=April 17, 2025|archive-url=https://web.archive.org/web/20250125042025/https://okazu.yuricon.com/2004/02/06/maria-sama-ga-miteru-thoughts-on-the-first-story-arc/|archive-date=January 25, 2025|url-status=live}}

| rowspan="2"|Japan

January 29, 2007

| Kintarou Arisugawa

| Trans woman

| Also known as Alice, she is enrolled in Hanadera Academy, and her inner female identity is at odds with her male appearance.{{cite web|url=http://www.gokigenyou.com/chara05.htm|title=■ 生徒紹介|trans-title=■ Student Introduction|publisher=Oyuki Konno/Shueisha|website=Lillian Jogakuen|archive-url=https://web.archive.org/web/20070904200141/http://www.gokigenyou.com/chara05.htm|archive-date=September 4, 2007|url-status=live|quote=Secretary of the student council of Hanadera Gakuin. called alice. A man with such a cute appearance that he calls himself a different gender.}} As such, she is overjoyed at the decision by Sachiko to swap the genders of those students playing in a school play. She later runs for a secretary position on the Student Council of Lillian Girls' Academy, as noted in the light novel.{{cite web|last=Friedman|first=Erica|url=https://okazu.yuricon.com/2013/06/09/light-novel-oshaka-sama-mo-miteru-kyoki-ippou-%e3%81%8a%e9%87%88%e8%bf%a6%e6%a7%98%e3%82%82%e3%81%bf%e3%81%a6%e3%82%8b-%e6%bd%94%e3%81%8d%e4%b8%80%e7%a5%a8/|title=Light Novel: Oshaka-sama mo Miteru ~ Kyoki Ippou (お釈迦様もみてる潔き一票)|website=Okazu|date=June 13, 2013|access-date=April 17, 2025|archive-url=https://web.archive.org/web/20190525052334/http://okazu.yuricon.com/2013/06/09/light-novel-oshaka-sama-mo-miteru-kyoki-ippou-%e3%81%8a%e9%87%88%e8%bf%a6%e6%a7%98%e3%82%82%e3%81%bf%e3%81%a6%e3%82%8b-%e6%bd%94%e3%81%8d%e4%b8%80%e7%a5%a8/|archive-date=May 25, 2019|url-status=live}}

2004—2010

| 6teen

| December 21, 2006

| Connie

| Lesbian

| A drummer in Wyatt's band who appears in a few episodes ("Snow Job," "Selling Out To The Burger Man," and "Role Reversal"). She goes with Jean to the Sadie Hawkins Day Dance.{{cite web|last=White|first=Abbey|url=https://www.insider.com/why-tv-lacks-lgbtq-inclusion-according-to-experts-2021-6|title=TV animators were forced to scrap LGBTQ-inclusive storylines due to a culture of fear. Experts say fans are changing that.|website=Insider|date=June 15, 2021|access-date=June 16, 2021|archive-url=https://archive.today/20210616194517/https://www.insider.com/why-tv-lacks-lgbtq-inclusion-according-to-experts-2021-6|archive-date=June 16, 2021|url-status=live}}{{efn|Jean is the third gay character to be featured on the show, she's the only one who's outright stated to be so, with the others being a possibly gay cowboy and Kevin as gay.}}

| Canada

2004—2012

| Bleach

| March 22, 2005

| Sui Feng

| Lesbian

| Sui, also known as Soifon and born with the name of Fēng Shāolíng, is the commander of the Onmitsukidō militia.{{cite web |last1=Kemner |first1=Louis |title=Bleach: 10 Things You Didn't Know About Captain Soi Fon |url=https://www.cbr.com/bleach-captain-soi-fon-facts-trivia/ |website=CBR |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20191215135603/https://www.cbr.com/bleach-captain-soi-fon-facts-trivia/ |archive-date=December 15, 2019 |date=November 5, 2019 |url-status=live |df=mdy-all}} During the series she has two partners she works with: a woman, Yoruichi Shihōin, who she mentored, and a man, Marechiyo Ōmaeda. However, she only shows affection toward Yoruichi, calling her "so noble, so beautiful and so terrifyingly strong" in the manga,Bleach manga; Chapter 159, page 9.{{better source needed|date=July 2024}} and remains protective of her, while Yoruichi flirts with her,{{cite web |last1=Necessary |first1=Terra |title=9 Queer Anime Couples Everyone Wishes Were Real |url=https://www.pride.com/geek/2019/11/21/9-queer-anime-couples-everyone-wishes-were-real |website=pride.com |publisher=Pride Media |access-date=June 30, 2020 |archive-url=https://web.archive.org/web/20200204224226/https://www.pride.com/geek/2019/11/21/9-queer-anime-couples-everyone-wishes-were-real |archive-date=February 4, 2020 |date=November 21, 2019 |url-status=live |df=mdy-all}} in episodes like "The Past Chapter Begins! The Truth from 110 Years Ago", As such, she is distressed when Yoruichi disappears, works to rebuild the Onmitsukidō militia.Bleach Official Character Book Souls, page 196.{{better source needed|date=July 2024}} and later fights with her face-to-face in the episodes "The Strongest Shinigami! Ultimate Confrontation Between Teacher and Students" and "Supersonic Battle! Determine the Goddess of Battle."{{cite web |last1=Kemner |first1=Louis |title=Bleach: 5 Characters Captain Soi Fon Can Beat (& 5 She'd Lose To) |url=https://www.cbr.com/bleach-captain-soi-characters-win-lose-fight/ |website=CBR |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20200412122604/https://www.cbr.com/bleach-captain-soi-characters-win-lose-fight/ |archive-date=April 12, 2020 |date=April 8, 2020 |url-status=live |df=mdy-all}} Sui later breaks down after hearing Yoruichi saying that she feels that Sui disappointed, betrayed, and abandoned her, and refuses to fight Yoruichi anymore,{{cite web |last1=Kemner |first1=Louis |title=Bleach: 10 Essential Facts About Yoruichi Shihoin |url=https://www.cbr.com/bleach-yoruichi-shihoin-facts-trivia/ |website=CBR |access-date=July 17, 2020 |archive-url=https://web.archive.org/web/20200630033441/https://www.cbr.com/bleach-yoruichi-shihoin-facts-trivia/ |archive-date=June 30, 2020 |date=February 3, 2020 |url-status=live |df=mdy-all}} as shown in the episode "Senbonzakura, Crushed! Zangetsu Thrusts through the Sky," and they become friends again, ultimately.

| Japan

rowspan="5"|2004–2018

| rowspan="5"|The Venture Bros.

| September 11, 2004

| Colonel Horace Gentleman

| rowspan="3"|Gay

| According to the creators of the show, Colonel Gentleman was always, openly, and proudly gay.{{cite web| url=https://www.huffpost.com/entry/the-venture-bros-talk-gay-characters_n_1659006|title='Venture Bros.' Co-Creators From Adult Swim Talk Gay Characters, The Upcoming Season And Gay Sex|work=Huffington Post|date=July 11, 2012|access-date=March 16, 2020|first=Kevin|last=Burra|archive-url=https://web.archive.org/web/20200412013714/https://www.huffpost.com/entry/the-venture-bros-talk-gay-characters_n_1659006|archive-date=April 12, 2020}} He has an ex-wife, Mz. Quymn, and a former lover, Kiki, shown in the episode "Past Tense," who he lived with at his home in Tangiers, Morocco for years.

| rowspan="4"|United States

September 3, 2006

| The Alchemist

| rowspan="2"|The Alchemist and Shore Leave are in an on-again, off-again relationship, shown in episodes such as "Fallen Arches." According to the show's creators, The Alchemist is gay in a way that is "just incidental" while Shore Leave is a very "openly swishy queer proud guy." They both appeared in The Venture Bros.: Radiant Is the Blood of the Baboon Heart.

June 25, 2008

| Shore Leave

July 9, 2006

| Hunter Gathers

| Genderqueer, genderfluid, or trans woman

| Brock Samson's most frequent commanding officer at the OSI. In the first episode the then-colonel Gathers is featured in, Brock is assigned the task of killing him for going AWOL; when he tracks Hunter down, Brock finds "he" has undergone sex reassignment surgery and now considers her a woman, who is therefore ineligible to be assassinated under the OSI's "no women, no children" policy.{{cite web |last1=Carroll |first1=Larry|title=The Venture Bros.: "Assassinanny 911" Review |website=IGN |url=https://www.ign.com/articles/2006/07/12/the-venture-bros-assassinanny-911-review |access-date=December 12, 2024 |date=July 12, 2006 }} Gathers later detransitions before officially returning to the OSI and being promoted to general. Although the fact that Hunter had wanted the sex change for over a decade is made abundantly clear, it is also revealed that the sex change was leveraged for the purpose of going undercover, adding ambiguity to Hunter's precise relationship to their gender. After detransitioning, masculine terms are once again used to refer to Gathers.

July 6, 2008

| Virginia "Ginnie" Dunne

| Lesbian or bisexual

| Dr. Quymn's very masculine bodyguard, a reported "man-hater."{{cite web |last1=Lowe |first1=Scott |title=The Venture Bros.: "Dr. Quymn, Medicine Woman" Review |website=IGN |url=https://www.ign.com/articles/2008/07/08/the-venture-bros-dr-quymn-medicine-woman-review |access-date=July 10, 2020 |archive-url=https://web.archive.org/web/20140419092814/https://www.ign.com/articles/2008/07/08/the-venture-bros-dr-quymn-medicine-woman-review |archive-date=April 19, 2014 |date=July 8, 2008 |url-status=live}} In the episode "Dr. Quymn, Medicine Woman," she is shown to be a lesbian{{cite web |last1=Reed |first1=Phil |title=The Venture Bros.: Dr. Quymn, Medicine Woman |website=Noise to Signal |url=http://noisetosignal.org/tv/2008/07/the-venture-bros-dr-quymn-medicine-woman.php.html |access-date=July 10, 2020 |archive-url=https://web.archive.org/web/20200604174530/http://noisetosignal.org/tv/2008/07/the-venture-bros-dr-quymn-medicine-woman.php.html |archive-date=June 4, 2020 |date=July 7, 2008 |url-status=live}}{{better source needed|date=July 2024}} and refers to herself as such through innuendo, including apparent attempts to "convert" the heterosexual Dr. Quymn. Although she claims to be attracted only to women, she is shown to have bisexual tendencies, teasing and nearly having sex with Brock Samson; prior to this, she suggests Brock was the first "real man" she had ever met and implies he may be the first man she had ever been attracted to.

rowspan="2"|2004—present

| rowspan="2"|Peppa Pig

| September 15, 2021

| Dr. Polar Bear

| rowspan="2"|Lesbian

| rowspan="2"|Dr Polar Bear and Mummy Polar Bear are the mothers of Penny Polar Bear.{{cite web|url=https://www.npr.org/2022/09/07/1121476913/thomas-and-friends-tank-engine-peppa-autistic-same-sex|title=Thomas the Tank Engine and Peppa Pig welcome friends with autism and 2 mommies|last1=Chappell|first1=Bill|website=NPR|date=September 7, 2022|access-date=September 7, 2022}}

| rowspan="2"|United Kingdom

November 19, 2021

| Mummy Polar Bear

Notes

References

=Citations=

=Sources=

{{cite report |ref={{sfnref|University of the Pacific|2004}} |first=Michele |last=Gibney |date=2004 |title=Homosexuality in Fushigi Yuugi and Gravitation: An investigation into the cultural background of homosexuality in Japanese animation |url=https://works.bepress.com/michele_gibney/28/ |publisher=University of the Pacific |access-date=July 13, 2020 }}

Category:2000s animated television series

Category:2000s-related lists

Category:Lists of LGBTQ-related television shows

Category:Lists of animated series

Category:2000 in LGBTQ history

Category:2001 in LGBTQ history

Category:2002 in LGBTQ history

Category:2003 in LGBTQ history

Category:2004 in LGBTQ history

Category:2000 in television

Category:2001 in television

Category:2002 in television