Rh blood group system#Rh factor

The Rh blood group system has two sets of nomenclature: one developed by Ronald Fisher and R. R. Race, the other by {{visible anchor|Wiener}}. The two systems reflect different theories of inheritance. The Fisher–Race system, which is currently more common, uses the CDE nomenclature. This system is based on the theory that a separate gene controls the product of each corresponding antigen (e.g., a "D gene" produces D antigen, and so on). However, the d gene was hypothetical, not actual.

The Wiener system uses the Rh–Hr nomenclature. This system is based on the theory that there is one gene at a single locus on each of the two copies of chromosome 1, each contributing to production of multiple antigens. In this theory, a gene R₁ is supposed to give rise to the "blood factors" Rh₀, rh′, and rh″ (corresponding to modern nomenclature of the D, C, and E antigens) and the gene r to produce hr′ and hr″ (corresponding to modern nomenclature of the c and e antigens).{{Cite journal |vauthors = Weiner AS |author-link=Alexander S. Weiner |title=Genetics and Nomenclature of the Rh–Hr Blood Types |journal=Antonie van Leeuwenhoek |volume=15 |issue=1 |pages=17–28 |date=1 February 1949 |issn=0003-6072 |doi=10.1007/BF02062626 |s2cid=35680084 }}

Notations of the two theories are used interchangeably in blood banking (e.g., Rho(D) meaning RhD positive). Wiener's notation is more complex and cumbersome for routine use. Because it is simpler to explain, the Fisher–Race theory has become more widely used.{{citation needed|date=December 2021}}

DNA testing has shown that both are partially correct: There are in fact two linked genes, the RHD gene which produces a single immune specificity (anti-D) and the RHCE gene with multiple specificities (anti-C, anti-c, anti-E, anti-e). Thus, Wiener's postulate that a gene could have multiple specificities (something many did not give credence to originally) has been proved to be correct. On the other hand, Wiener's theory that there is only one gene has proved to be incorrect, as has the Fisher–Race theory that there are three genes, rather than the two. The CDE notation used in the Fisher–Race nomenclature is sometimes rearranged to DCE to more accurately represent the co-location of the C and E encoding on the RhCE gene, and to make interpretation easier.{{citation needed|date=December 2021}}

The proteins which carry the Rh antigens are transmembrane proteins, whose structure suggests that they are ion channels.{{cite web |url=https://www.ncbi.nlm.nih.gov/gv/mhc/xslcgi.cgi?cmd=bgmut/systems_info&system=rh |title=dbRBC – Blood Group Antigen Gene Mutation Database |publisher=www.ncbi.nlm.nih.gov |access-date=2010-06-15 |url-status=live |archive-url=https://web.archive.org/web/20110213004536/https://www.ncbi.nlm.nih.gov/gv/mhc/xslcgi.cgi?cmd=bgmut%2Fsystems_info&system=rh |archive-date=2011-02-13 }} The main antigens are D, C, E, c and e, which are encoded by two adjacent gene loci, the RHD gene which encodes the RhD protein with the D antigen (and variants){{cite web|title=RHD Rh blood group, D antigen [Homo sapiens] – Gene Result|url=https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=6007|access-date=2010-06-15|publisher=nlm.nih.gov}} and the RHCE gene which encodes the RhCE protein with the C, E, c and e antigens (and variants).{{cite web |url=https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6006&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum |title=RHCE Rh blood group, CcEe antigens [Homo sapiens] – Gene Result |publisher=nlm.nih.gov |access-date=2010-06-15 |url-status=live |archive-url=https://web.archive.org/web/20100320100830/https://www.ncbi.nlm.nih.gov/sites/entrez?db=gene |archive-date=2010-03-20 }} There is no d antigen. Lowercase "d" indicates the absence of the D antigen (the gene is usually deleted or otherwise nonfunctional).{{citation needed|date=December 2021}}

File:Rh blood cells drawing.svg

Rh phenotypes are readily identified through the presence or absence of the Rh surface antigens. As can be seen in the table below, most of the Rh phenotypes can be produced by several different Rh genotypes. The exact genotype of any individual can only be identified by DNA analysis. Regarding patient treatment, only the phenotype is usually of any clinical significance to ensure a patient is not exposed to an antigen they are likely to develop antibodies against. A probable genotype may be speculated on, based upon the statistical distributions of genotypes in the patient's place of origin.{{citation needed|date=December 2021}}

R₀ (cDe or Dce) is today most common in Africa. The allele was thus often assumed in early blood group analyses to have been typical of populations on the continent, particularly in areas below the Sahara. Ottensooser et al. (1963) suggested that high R₀ frequencies were likely characteristic of the ancient Judean Jews, who had emigrated from Egypt prior to their dispersal throughout the Mediterranean Basin and Europe{{cite journal |vauthors = Ottensooser F, Leon N, Sato M, Saldanha PH |title = Blood groups of a population of Ashkenazi Jews in Brazil |journal = American Journal of Physical Anthropology |volume = 21 |issue = 1 |pages = 41–8 |date = March 1963 |pmid = 13940710 |doi = 10.1002/ajpa.1330210106 |s2cid = 21795826 }} on the basis of high R₀ percentages among Sephardi and Ashkenazi Jews compared to native European populations and the relative genetic isolation of Ashkenazim. However, more recent studies have found R₀ frequencies as low as 24.3% among some Afroasiatic-speaking groups in the Horn of Africa,{{cite journal| vauthors = Harrison GA, Küchemann CF, Moore MA, Boyce AJ, Baju T, Mourant AE, Godber MJ, Glasgow BG, Kopeć AC, Tills D, Clegg EJ |title=The effects of altitudinal variation in Ethiopian populations|journal=Philosophical Transactions of the Royal Society of London B: Biological Sciences|date=1969|volume=256|issue=805|pages=147–182|doi=10.1098/rstb.1969.0040|bibcode=1969RSPTB.256..147H|doi-access=}} as well as higher R₀ frequencies among certain other Afroasiatic speakers in North Africa (37.3%){{cite journal| vauthors = Metri AA, Sidi-Yakhlef A, Biémont C, Saidi M, Chaif O, Ouraghi SA |title=A genetic study of nine populations from the region of Tlemcen in Western Algeria: a comparative analysis on the Mediterranean scale |journal=Anthropological Science |date=2012 |volume=120 |issue=3 |pages=209–216 |url=https://www.jstage.jst.go.jp/article/ase/120/3/120_120618/_html |access-date=28 August 2017 |doi=10.1537/ase.120618 |url-status=live |archive-url=https://web.archive.org/web/20170829120702/https://www.jstage.jst.go.jp/article/ase/120/3/120_120618/_html |archive-date=29 August 2017 |doi-access=free}} and among some Palestinians in the Levant (30.4%).{{cite journal |vauthors = El-Wahhab Skaik YA |title = The Rh allele frequencies in Gaza city in Palestine |journal = Asian Journal of Transfusion Science |volume = 5 |issue = 2 |pages = 150–2 |date = July 2011 |pmid = 21897594 |pmc = 3159245 |doi = 10.4103/0973-6247.83241 |doi-access = free }} On the contrary, at a frequency of 47.2% of the population of Basque country having the lack of the D antigen, these people display the highest frequency of the Rh negative phenotype.{{cite journal |vauthors = Flores-Bello A, Mas-Ponte D, Rosu ME, Bosch E, Calafell F, Comas D |title = Sequence diversity of the Rh blood group system in Basques |journal = European Journal of Human Genetics |volume = 26 |issue = 12 |pages = 1859–1866 |date = December 2018 |pmid = 30089826 |pmc = 6244411 |doi = 10.1038/s41431-018-0232-1 }}

• Figures taken from a study performed in 1948 on a sample of 2000 people in the United Kingdom.{{cite journal |vauthors = Race RR, Mourant AE |title = The Rh chromosome frequencies in England |journal = Blood |volume = 3 |issue = 6 |pages = 689–95 |date = June 1948 |pmid = 18860341 |doi = 10.1182/blood.V3.6.689.689 |doi-access = free }}

Rh antibodies are Immunoglobulin G (IgG) antibodies which are acquired through exposure to Rh-positive blood (generally either through pregnancy or transfusion of blood products). The D antigen is the most immunogenic of all the non-ABO antigens. Approximately 80% of individuals who are D-negative and exposed to a single D-positive unit will produce an anti-D antibody. The percentage of alloimmunization is significantly reduced in patients who are actively exsanguinating.{{cite book | veditors = Roback JR , Combs MR , Grossman BJ , Hillyer CD | title = AABB Technical Manual | edition = 16th | location = Bethesda | publisher = AABB Press | date = 2008 }}

All Rh antibodies except D display dosage (antibody reacts more strongly with red cells homozygous for an antigen than cells heterozygous for the antigen (EE stronger reaction vs Ee)).

If anti-E is detected, the presence of anti-c should be strongly suspected (due to combined genetic inheritance). It is therefore common to select c-negative and E-negative blood for transfusion patients who have an anti-E and lack the c antigen (in general, a patient will not produce antibodies against their own antigens). Anti-c is a common cause of delayed hemolytic transfusion reactions.

{{Main|Hemolytic disease of the newborn}}

The hemolytic condition occurs when there is an incompatibility between the blood types of the mother and fetus. There is also potential incompatibility if the mother is Rh negative, and the father is positive. When the mother conceives for the first time, with a positive child, she will become extremely sensitive. When any incompatibility is detected when she conceives the second time in less than two years then, the mother often receives an injection at 28 weeks' gestation and at birth to avoid the development of antibodies towards the fetus. If not given, then the baby will be dead and must be aborted. These terms do not indicate which specific antigen-antibody incompatibility is implicated. The disorder in the fetus due to Rh D incompatibility is known as erythroblastosis fetalis.

• Hemolytic comes from two words: "hema" (blood) and "lysis" (solution) or breaking down of red blood cells
• Erythroblastosis refers to the making of immature red blood cells
• Fetalis refers to the fetus.

When the condition is caused by the Rh D antigen-antibody incompatibility, it is called Rh D Hemolytic disease of the newborn or Rh disease. Here, sensitization to Rh D antigens (usually by feto-maternal transfusion during pregnancy) may lead to the production of maternal IgG anti-D antibodies which can pass through the placenta. This is of particular importance to D negative females at or below childbearing age, because any subsequent pregnancy may be affected by the Rh D hemolytic disease of the newborn if the baby is D positive. The vast majority of Rh disease is preventable in modern antenatal care by injections of IgG anti-D antibodies (Rho(D) Immune Globulin). The incidence of Rh disease is mathematically related to the frequency of D negative individuals in a population, so Rh disease is rare in old-stock populations of Africa and the eastern half of Asia, and the Indigenous peoples of Oceania and the Americas, but more common in other genetic groups, most especially Western Europeans, but also other West Eurasians, and to a lesser degree, native Siberians, as well as those of mixed-race with a significant or dominant descent from those (e.g. the vast majority of Latin Americans and Central Asians).

• Symptoms and signs in the fetus:
• Enlarged liver, spleen, or heart and fluid buildup in the fetus' abdomen seen via ultrasound.
• Symptoms and signs in the newborn:
• Anemia that creates the newborn's pallor (pale appearance).
• Jaundice or yellow discoloration of the newborn's skin, sclera or mucous membrane. This may be evident right after birth or after 24–48 hours after birth. This is caused by bilirubin (one of the end products of red blood cell destruction).
• Enlargement of the newborn's liver and spleen.
• The newborn may have severe edema of the entire body.
• Dyspnea (difficulty breathing)

Rh disease only occurs in human fetuses, however a similar disease called Neonatal isoerythrolysis (NI) can be observed in animal species of newborn horses, mules, pigs, cats, cattle, and dogs.  What differs between Rh disease and NI is the pathogenesis of hemolysis between human fetuses and the animal species.  With human mothers, the maternal antibodies are formed from the sensitization of foreign antigens of her unborn fetus's red blood cells passing through the placenta causing hemolysis before birth. With other animals, however, these maternal antibodies are not passed through the placenta, but through colostrum.  The newborn animal is without NI but soon develops hemolytic anemia after initial ingestion of its mother's colostrum that contain antibodies that can be absorbed through the newborn's intestines and are incompatible to its red blood cell antigen. After 48 hours of birth, the newborn may be allowed to nurse from its mother as her antibodies can no longer be absorbed through the neonate's intestines.  Because the most active newborn animals consume the most colostrum, they may be the ones who are most affected by the blood incompatibility of antigen and antibody.{{Cite book |vauthors = Cotter S |title=Hematology |series = Quick Look Series |publisher=Teton NewMedia |date=June 1, 2001 |isbn=978-1893441361 |edition=1st |pages=32–33}}

{{See also|Blood_type_distribution_by_country#ABO_and_Rh_distribution_by_country}}

According to a comprehensive study, the worldwide frequency of Rh-positive and Rh-negative blood types is approximately 94% and 6%, respectively. The same study concluded that the share of the population with Rh-negative blood type is set to fall further in the future primarily due to low population growth in Europe.{{Cite web |url=https://www.rhesusnegative.net/themission/bloodtypefrequencies/ |title=Blood Type Frequencies by Country including the Rh Factor | work = Rhesus Negative}} The frequency of Rh factor blood types and the RhD neg allele gene differs in various populations.{{citation needed|date=December 2021}}

File:Rh blood inheritance.svg for Rh factor inheritance. This square specifically shows two heterozygous Rh positive parents and the possible genotypes/phenotypes the offspring could have.]]

The D antigen is inherited as one gene (RHD) (on the short arm of the first chromosome, p36.13–p34.3) with various alleles. Typically, Rhesus positive people have an intact RHD gene while negative people lack the gene (or have mutations in it). However, there are exceptions: for instance, Japanese and black Africans may have an intact gene that is not expressed or only at very low levels.{{cite journal |vauthors = Avent ND, Reid ME |title = The Rh blood group system: a review |journal = Blood |volume = 95 |issue = 2 |pages = 375–387 |date = January 2000 |pmid = 10627438 |doi = 10.1182/blood.V95.2.375 |s2cid = 13803474 }} The gene codes for the RhD protein on the red blood cell membrane. D− individuals who lack a functional RHD gene do not produce the D antigen and may be immunized by D+ blood.{{citation needed|date=December 2021}}

The D antigen is a dominant trait. If both of a child's parents are Rh negative, the child will definitely be Rh negative. Otherwise, the child may be Rh positive or Rh negative, depending on the parents' specific genotypes.{{cite web |title=ABO inheritance patterns |url=https://www.transfusion.com.au/?q=node/77 |work=Inheritance patterns of blood groups |publisher=Australian Red Cross Blood Service |access-date=30 October 2013 |url-status=live |archive-url=https://web.archive.org/web/20131101224734/https://www.transfusion.com.au/?q=node%2F77 |archive-date=1 November 2013 }}

The epitopes for the next 4 most common Rh antigens, C, c, E and e are expressed on the highly similar RhCE protein that is genetically encoded in the RHCE gene, also found on chromosome 1. It has been shown that the RHD gene arose by duplication of the RHCE gene during primate evolution. Mice have just one RH gene.{{cite journal |vauthors = Wagner FF, Flegel WA |title = RHCE represents the ancestral RH position, while RHD is the duplicated gene |journal = Blood |volume = 99 |issue = 6 |pages = 2272–3 |date = March 2002 |pmid = 11902138 |doi = 10.1182/blood-2001-12-0153 |doi-access = free }}

The RHAG gene, which is responsible for encoding Rh-associated glycoprotein (RhAG), is found on chromosome 6a.

The polypeptides produced from the RHD and RHCE genes form a complex on the red blood cell membrane with the Rh-associated glycoprotein.{{cite book | vauthors = Mais DD |title=Quick Compendium of Clinical Pathology |date=2007 | location = Chicago |publisher=American Society for Clinical Pathology Press |isbn=978-0-89189-567-1 |edition=2nd}}

{{Pfam box

|Name=Blood group Rh C/E/D polypeptide

|InterPro=IPR002229

|TCDB=1.A.11

}}

On the basis of structural homology it has been proposed that the product of RHD gene, the RhD protein, is a membrane transport protein of uncertain specificity (CO₂ or NH₃) and unknown physiological role.{{cite journal |vauthors = Kustu S, Inwood W |title = Biological gas channels for NH3 and {{CO2}}: evidence that Rh (Rhesus) proteins are {{CO2}} channels |journal = Transfusion Clinique et Biologique |volume = 13 |issue = 1–2 |pages = 103–10 |year = 2006 |pmid = 16563833 |doi = 10.1016/j.tracli.2006.03.001 }}{{cite journal |vauthors = Biver S, Scohy S, Szpirer J, Szpirer C, André B, Marini AM |title = Physiological role of the putative ammonium transporter RhCG in the mouse |journal = Transfusion Clinique et Biologique |volume = 13 |issue = 1–2 |pages = 167–8 |year = 2006 |pmid = 16564721 |doi = 10.1016/j.tracli.2006.03.003 }} The three-dimensional structure of the related RHCG protein and biochemical analysis of the RhD protein complex indicates that the RhD protein is one of three subunits of an ammonia transporter.{{cite journal |vauthors = Gruswitz F, Chaudhary S, Ho JD, Schlessinger A, Pezeshki B, Ho CM, Sali A, Westhoff CM, Stroud RM |title = Function of human Rh based on structure of RhCG at 2.1 A |journal = Proceedings of the National Academy of Sciences of the United States of America |volume = 107 |issue = 21 |pages = 9638–43 |date = May 2010 |pmid = 20457942 |pmc = 2906887 |doi = 10.1073/pnas.1003587107 |bibcode = 2010PNAS..107.9638G |doi-access = free }}{{cite journal |vauthors = Westhoff CM |title = The structure and function of the Rh antigen complex |journal = Seminars in Hematology |volume = 44 |issue = 1 |pages = 42–50 |date = January 2007 |pmid = 17198846 |pmc = 1831834 |doi = 10.1053/j.seminhematol.2006.09.010 }} Three recent studies{{cite journal |vauthors = Novotná M, Havlícek J, Smith AP, Kolbeková P, Skallová A, Klose J, Gasová Z, Písacka M, Sechovská M, Flegr J |title = Toxoplasma and reaction time: role of toxoplasmosis in the origin, preservation and geographical distribution of Rh blood group polymorphism |journal = Parasitology |volume = 135 |issue = 11 |pages = 1253–61 |date = September 2008 |pmid = 18752708 |doi = 10.1017/S003118200800485X |s2cid = 5956901 }}{{cite journal |vauthors = Flegr J, Novotná M, Lindová J, Havlícek J |title = Neurophysiological effect of the Rh factor. Protective role of the RhD molecule against Toxoplasma-induced impairment of reaction times in women |journal = Neuro Endocrinology Letters |volume = 29 |issue = 4 |pages = 475–81 |date = August 2008 |pmid = 18766148 |url = https://natur.cuni.cz/flegr/pdf/rh2.pdf }}{{cite journal |vauthors = Flegr J, Klose J, Novotná M, Berenreitterová M, Havlícek J |title = Increased incidence of traffic accidents in Toxoplasma-infected military drivers and protective effect RhD molecule revealed by a large-scale prospective cohort study |journal = BMC Infectious Diseases |volume = 9 |pages = 72 |date = May 2009 |pmid = 19470165 |pmc = 2692860 |doi = 10.1186/1471-2334-9-72 |doi-access = free }} have reported a protective effect of the RhD-positive phenotype, especially RhD heterozygosity, against the negative effect of latent toxoplasmosis on psychomotor performance in infected subjects. RhD-negative compared to RhD-positive subjects without anamnestic titres of anti-Toxoplasma antibodies have shorter reaction times in tests of simple reaction times. And conversely, RhD-negative subjects with anamnestic titres (i.e. with latent toxoplasmosis) exhibited much longer reaction times than their RhD-positive counterparts. The published data suggested that only the protection of RhD-positive heterozygotes was long term in nature; the protection of RhD-positive homozygotes decreased with duration of the infection while the performance of RhD-negative homozygotes decreased immediately after the infection. The overall change in reaction times was always larger in the RhD-negative group than in the RhD-positive.{{citation needed|date=December 2021}}

{{cleanup section|reason=Should not be here, where we deal with the non-glycosylated variable Rh antigens; there should be an article describing all the Rh proteins (including RHAG, RHBG, RHCG, RHD, RHCE]], and that's where it should go.|date=January 2024}}

Rh-like proteins can be found even in species other than vertebrates (which have red blood cells) – worms, bacteria, and algae. All these Rh proteins have the same biochemical function of transporting {{CO2}}, differing slightly in their amino acid sequences. The Rh family as a whole is related to ammonia transporters (Amt). In C. elegans worms, disruption of the Rh1 gene causes growth defects under high {{CO2}} levels. Chlamydomonas reinhardtii algae fail to grow rapidly if its Rh gene is knocked down. Although in vitro evidence shows the Rh complex is capable of moving ammonia, its disruption does not cause growth defects under modified ammonia levels.{{cite journal |vauthors = Ji Q, Hashmi S, Liu Z, Zhang J, Chen Y, Huang CH |title = CeRh1 (rhr-1) is a dominant Rhesus gene essential for embryonic development and hypodermal function in Caenorhabditis elegans |journal = Proceedings of the National Academy of Sciences of the United States of America |volume = 103 |issue = 15 |pages = 5881–5886 |date = April 2006 |pmid = 16595629 |pmc = 1458667 |doi = 10.1073/pnas.0600901103 |bibcode = 2006PNAS..103.5881J |doi-access = free }}{{Cite web |date=2018-11-20 |title=What is Rhesus factor? Did we get it from Rhesus monkeys? Can Rh factor be found in other animals? |url=https://www.thetech.org/ask-a-geneticist/rhesus-factor-evolution | vauthors = Cho SE |access-date=2022-10-24 |website=The Tech Interactive |language=en}}

For a long time, the origin of RHD polymorphism was an evolutionary enigma.{{cite journal |vauthors = Haldane JB |title=Selection against heterozygosis in Man. |journal= Annals of Eugenics|volume=11 |pages=333–340 |year=1942 |doi=10.1111/j.1469-1809.1941.tb02297.x|doi-access=free }}{{cite journal |vauthors=Fisher RA, Race RR, Taylor GL |title=Mutation and the Rhesus reaction |journal=Nature |volume=153 |issue=3873 |pages=106 |year=1944 |doi=10.1038/153106b0|bibcode=1944Natur.153..106F |s2cid=2104065 |doi-access=free }}{{cite journal |vauthors = Li CC |title=Is the Rh facing a crossroad? A critique of the compensation effect. |journal=Am Nat |volume=87 |issue=835 |pages=257–261 |year=1953 |doi=10.1086/281782 |bibcode=1953ANat...87..257L |s2cid=84715943 }} Before the advent of modern medicine, the carriers of the rarer allele (e.g. RhD-negative women in a population of RhD positives or RhD-positive men in a population of RhD negatives) were at a disadvantage as some of their children (RhD-positive children born to preimmunised RhD-negative mothers) were at a higher risk of fetal or newborn death or health impairment from hemolytic disease.

Natural selection aside, the RHD-RHCE region is structurally predisposed to many mutations seen in humans, since the pair arose by gene duplication and remain similar enough for unequal crossing over to occur. In addition to the case where D is deleted, crossover can also produce a single gene mixing exons from both RHD and RHCE, forming the majority of partial D types.{{rp|323}}

In serologic testing, D positive blood is easily identified. Units that are D negative are often retested to rule out a weaker reaction. This was previously referred to as D^u, which has been replaced.{{cite book |vauthors = Brecher ME |title=Technical Manual |publisher=American Association of Blood Banks |location=Bethesda MD |year=2005 |isbn=978-1-56395-196-1 |edition=15th}}{{rp|322}} By definition, weak D phenotype is characterized by negative reaction with anti-D reagent at immediate spin (IS), negative reaction after 37 °C incubation, and positive reaction at anti-human globulin (AHG) phase. Weak D phenotype can occur in several ways. In some cases, this phenotype occurs because of an altered surface protein that is more common in people of European descent. An inheritable form also occurs, as a result of a weakened form of the R0 gene. Weak D may also occur as "C in trans", whereby a C gene is present on the opposite chromosome to a D gene (as in the combination R0r', or "Dce/dCe"). The testing is difficult, since using different anti-D reagents, especially the older polyclonal reagents, may give different results.

The practical implication of this is that people with this sub-phenotype will have a product labeled as "D positive" when donating blood. When receiving blood, they are sometimes typed as a "D negative", though this is the subject of some debate. Most "Weak D" patients can receive "D positive" blood without complications.{{rp|323}} However, it is important to correctly identify the ones that have to be considered D+ or D−. This is important, since most blood banks have a limited supply of "D negative" blood and the correct transfusion is clinically relevant. In this respect, genotyping of blood groups has much simplified this detection of the various variants in the Rh blood group system.

It is important to differentiate weak D (due to a quantitative difference in the D antigen) from partial D (due to a qualitative difference in the D antigen). Simply put, the weak D phenotype is due to a reduced number of D antigens on a red blood cell. In contrast, the partial D phenotype is due to an alteration in D-epitopes. Thus, in partial D, the number of D antigens is not reduced but the protein structure is altered. These individuals, if alloimmunized to D, can produce an anti-D antibody. Therefore, partial D patients who are donating blood should be labeled as D-positive but, if receiving blood, they should be labeled as D-negative and receive D-negative units.

In the past, partial D was called 'D mosaic' or 'D variant.' Different partial D phenotypes are defined by different D epitopes on the outer surface of the red blood cell membrane. More than 30 different partial D phenotypes have been described.

Rh_null individuals have no Rh antigens (no Rh or RhAG) on their red blood cells.{{cite journal |vauthors = Cartron JP |title = RH blood group system and molecular basis of Rh-deficiency |journal = Baillière's Best Practice & Research. Clinical Haematology |volume = 12 |issue = 4 |pages = 655–89 |date = December 1999 |pmid = 10895258 |doi = 10.1053/beha.1999.0047 }} This rare condition has been called "Golden Blood".{{Cite web|url=https://curiosity.com/topics/rhnull-the-rarest-blood-type-on-earth-has-been-called-the-golden-blood-curiosity/ |archive-url=https://web.archive.org/web/20191205020106/https://curiosity.com/topics/rhnull-the-rarest-blood-type-on-earth-has-been-called-the-golden-blood-curiosity/ |archive-date=5 December 2019 |title=Rhnull, the Rarest Blood Type on Earth, Has Been Called the "Golden Blood"|website=Curiosity.com|access-date=2019-06-05| vauthors = Faletto J |date=9 September 2017}} As a consequence of Rh antigen absence, Rh_null red blood cells also lack LW and Fy5 and show weak expression of S, s, and U antigens.

Red blood cells lacking Rh/RhAG proteins have structural abnormalities (such as stomatocytosis) and cell membrane defects that can result in hemolytic anemia.

The first Rh_null blood was discovered in an Aboriginal Australian woman, in 1961.{{Cite journal | vauthors = Hasekura H, Boettcher B | title = Family study of the original Australian Rh-null. | journal = Proceedings of the Japan Academy | date = 1970 | volume = 46 | issue = 7 | pages = 733–737 | via = J-STAGE |url=https://www.jstage.jst.go.jp/article/pjab1945/46/7/46_7_733/_pdf }} Only 43 individuals have been reported to have it worldwide. Only nine active donors have been reported. Its properties make it attractive in numerous medical applications, but scarcity makes it expensive to transport and acquire.{{Cite web | vauthors = Bailey P |date=2014-10-21 |title=The man with the golden blood |url=https://medium.com/mosaic-science/the-man-with-the-golden-blood-b91e8c8c22d9 |access-date=2024-09-22 |website=Mosaic science |language=en}}

As of 2023, over 50 antigens have been described in the Rh group system; among those described here, the D, C, c, E and e antigens are the most important. The others are much less frequently encountered or are rarely clinically significant. Each is given a number, though the highest assigned number (CEVF or RH61 according to the ISBT terminology) is not an accurate reflection of the antigens encountered since many (e.g. Rh38) have been combined, reassigned to other groups, or otherwise removed.{{rp|324}}

Some of the other Rh "antigens" are f ("ce", RH6), Ce (RH7), C^w (RH8), C^x (RH9), V (RH10), E^w (RH11), G (RH12), Tar (RH40), VS (RH20), D^w (RH23), and CE (RH22). Some of these groups, including f, Ce and CE, describe grouping of some existing groups. Others, like V, describe an epitope created by some other mutation on the RHD and RHCE genes. V in particular is caused by a mutation on RHCE.{{cite web | vauthors = Meunier D, Peng S, Clarke G | location = Ottawa | publisher = Canadian Blood Services | date = 25 September 2019 |title=Rh System: Anti-V |url=https://professionaleducation.blood.ca/en/transfusion/bonnes-pratiques/pratiques-suivre-en-matiere-de-serologie/rh-system-anti-v |archive-url=https://web.archive.org/web/20201107185212/https://professionaleducation.blood.ca/en/transfusion/best-practices/serological-best-practices/rh-system-anti-v |archive-date=7 November 2020 |website=Professional Education |language=en }}

The term "Rh" was originally an abbreviation of "Rhesus factor". It was discovered in 1939 by Karl Landsteiner and Alexander S. Wiener, who, at the time, believed it to be a similar antigen found in rhesus macaque red blood cells. It was subsequently discovered that the human factor is not identical to the rhesus monkey factor, but by then, "Rhesus Group" and like terms were already in widespread, worldwide use. Thus, notwithstanding it is a misnomer, the term survives (e.g., rhesus blood group system and the obsolete terms rhesus factor, rhesus positive, and rhesus negative – all three of which actually refer specifically and only to the Rh D factor and are thus misleading when unmodified). Contemporary practice is to use "Rh" as a term of art instead of "Rhesus" (e.g., "Rh Group", "Rh factors", "Rh D", etc.).

The significance of their discovery was not immediately apparent and was only realized in 1940, after subsequent findings by Philip Levine and Rufus Stetson.{{cite journal |vauthors = Landsteiner K, Wiener AS |year=1940 |title=An Agglutinable Factor in Human Blood Recognized by Immune Sera for Rhesus Blood |journal=Exp Biol Med (Maywood) |volume=43 |issue=1 |pages=223 |doi=10.3181/00379727-43-11151 |s2cid=58298368 }} The serum that led to the discovery was produced by immunizing rabbits with red blood cells from a rhesus macaque. The antigen that induced this immunization was designated by them as Rh factor to indicate that rhesus blood had been used for the production of the serum.{{cite journal |vauthors = Landsteiner K, Wiener AS |title = Studies on an agglutinogen (Rh) in human blood reacting with anti-rhesus sera and with human isoantibodies |journal = The Journal of Experimental Medicine |volume = 74 |issue = 4 |pages = 309–20 |date = September 1941 |pmid = 19871137 |pmc = 2135190 |doi = 10.1084/jem.74.4.309 }}

In 1939, Phillip Levine and Rufus Stetson published in a first case report the clinical consequences of non-recognized Rh factor, hemolytic transfusion reaction, and hemolytic disease of the newborn in its most severe form.{{cite journal |vauthors=Levine P, Stetson RE |title=An unusual case of intragroup agglutination |journal=JAMA |volume=113 |issue= 2|pages=126–7 |year=1939 |doi=10.1001/jama.1939.72800270002007a}} It was recognized that the serum of the reported woman agglutinated with red blood cells of about 80% of the people although the then known blood groups, in particular ABO were matched. No name was given to this agglutinin when described. In 1940, Landsteiner and Wiener made the connection to their earlier discovery, reporting a serum that also reacted with about 85% of different human red blood cells.{{cite journal |vauthors=Landsteiner K, Wiener AS |title=An agglutinable factor in human blood recognized by immune sera for rhesus blood |journal=Proc Soc Exp Biol Med |volume=43 |pages=223–4 |year=1940 |doi=10.3181/00379727-43-11151|s2cid=58298368 }}

In 1941, Group O: a patient in Irvington, New Jersey, US, delivered a normal{{clarify|date=July 2023}} infant in 1931; this pregnancy was followed by a long period of sterility. The second pregnancy (April, 1941) resulted in an infant with icterus gravis.{{cite journal| vauthors = Levine P, Burnham L, Katzin E, Vogel P |date=December 1941|title=The role of iso-immunization in the pathogenesis of erythroblastosis fetalis |journal=American Journal of Obstetrics and Gynecology|language=en|volume=42|issue=6|pages=925–937|doi=10.1016/S0002-9378(41)90260-0|issn=0002-9378}} In May 1941, the third anti-Rh serum (M.S.) of Group O became available.

Based on the serologic similarities, 'Rh factor' was later also used for antigens, and anti-Rh for antibodies, found in humans such as those previously described by Levine and Stetson. Although differences between these two sera were shown already in 1942 and clearly demonstrated in 1963, the already widely used term "Rh" was kept for the clinically described human antibodies which are different from the ones related to the rhesus monkey. This real factor found in rhesus macaque was classified in the Landsteiner-Weiner antigen system (antigen LW, antibody anti-LW) in honor of the discoverers.{{cite journal |vauthors = Avent ND, Reid ME |title = The Rh blood group system: a review |journal = Blood |volume = 95 |issue = 2 |pages = 375–87 |date = January 2000 |pmid = 10627438 |doi = 10.1182/blood.V95.2.375 |s2cid = 13803474 }}{{cite journal |vauthors = Scott ML |title = The complexities of the Rh system |journal = Vox Sanguinis |volume = 87 |issue = Suppl. 1 |pages = 58–62 |date = July 2004 |pmid = 15200606 |doi = 10.1111/j.1741-6892.2004.00431.x |s2cid = 42103877 }}

It was recognized that the Rh factor was just one in a system of various antigens. Based on different models of genetic inheritance, two different terminologies were developed; both of them are still in use.

The clinical significance of this highly immunizing D antigen (i.e., Rh factor) was soon realized. Some keystones were to recognize its importance for blood transfusion (including reliable diagnostic tests), hemolytic disease of the newborn (including exchange transfusion), and very importantly the prevention of it by screening and prophylaxis.

The discovery of cell-free fetal DNA in maternal circulation by Holzgrieve et al. led to the noninvasive genotyping of fetal Rh genes in many countries.

{{reflist}}

• [https://www.ncbi.nlm.nih.gov/gv/mhc/xslcgi.cgi?cmd=bgmut/systems_info&system=rh Rh] at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH

{{transfusion medicine}}

Category:Blood

Category:Blood antigen systems

Category:Transfusion medicine